Abstract:
:Immunoglobulin genes contain a conserved eight base sequence element 5' to the site of transcription initiation. This octamer can serve as a site for the binding of nuclear proteins which are presumably involved in the cell type specific expression of this family of genes. In studying the binding of nuclear proteins to this conserved sequence element, we have detected a protein interaction that involves, in addition to the octamer, nucleotides which are immediately upstream. We have characterized this additional contact as a sequence specific interaction with a heptameric sequence element (CTCATGA) that is conserved among Ig heavy chain promoters. Protein binding to the heptamer is unique in that it is dependent upon the proximity and orientation of, as well as protein interaction with, the conserved octamer.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Landolfi NF,Yin XM,Capra JD,Tucker PWdoi
10.1093/nar/16.12.5503subject
Has Abstractpub_date
1988-06-24 00:00:00pages
5503-14issue
12eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::An unexpectedly high number of regulatory RNAs have been recently discovered that fine-tune the function of genes at all levels of expression. We employed Genomic SELEX, a method to identify protein-binding RNAs encoded in the genome, to search for further regulatory RNAs in Escherichia coli. We used the global regula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq032
更新日期:2010-06-01 00:00:00
abstract::Protein domains are subunits of proteins that recur throughout the protein world. There are many definitions attempting to capture the essence of a protein domain, and several systems that identify protein domains and classify them into families. EVEREST, recently described in Portugaly et al. (2006) BMC Bioinformatic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl850
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.7.2407
更新日期:1977-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt961
更新日期:2014-01-01 00:00:00
abstract::A 34 base-pair (bp) fragment spanning sequences -154 to -120 of the promoter of the CHO1 gene (structural gene for phosphatidylserine synthase) from the yeast Saccharomyces cerevisiae has been shown to place transcription of a promoter-less Escherichia coli lacZ gene under control of the phospholipid precursors inosit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.6.1411
更新日期:1992-03-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.18.3706
更新日期:1994-09-11 00:00:00
abstract::The YaeJ protein is a codon-independent release factor with peptidyl-tRNA hydrolysis (PTH) activity, and functions as a stalled-ribosome rescue factor in Escherichia coli. To identify residues required for YaeJ function, we performed mutational analysis for in vitro PTH activity towards rescue of ribosomes stalled on ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1280
更新日期:2014-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.23.9707
更新日期:1987-12-10 00:00:00
abstract::Prion proteins (PrPs) cause prion diseases, such as bovine spongiform encephalopathy. The conversion of a normal cellular form (PrP(C)) of PrP into an abnormal form (PrP(Sc)) is thought to be associated with the pathogenesis. An RNA aptamer that tightly binds to and stabilizes PrP(C) is expected to block this conversi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1132
更新日期:2013-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz900
更新日期:2019-12-02 00:00:00
abstract::Nucleoside 3'-phosphoramidite and chlorophosphite reagents have been found to react with the lactam function of guanine. This reaction caused unsatisfactory results when oligodeoxyribonucleotides containing a large number of guanine bases were prepared in an automated solid phase synthesizer. The guanine modification ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.18.6447
更新日期:1985-09-25 00:00:00
abstract::The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease in insulin levels occurs due to a decrease in insulin translation. Since regulation at the translational level is generally mediated through RNA-binding ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky867
更新日期:2018-12-14 00:00:00
abstract::The 2'-5' oligoadenylate synthetases form a well conserved family of interferon induced proteins, presumably present throughout the mammalian class. Using the Expressed Sequence Tag databases, we have identified a novel member of this family. This protein, which we named p59 2'-5' oligoadenylate synthetase-like protei...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.18.4121
更新日期:1998-09-15 00:00:00
abstract::Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin). Import/processing assays indicate that D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg272
更新日期:2003-03-15 00:00:00
abstract::The HIV RT and Protease Sequence Database is an online relational database that catalogs evolutionary and drug-related human immunodeficiency virus (HIV) reverse transcriptase (RT) and protease sequence variation (http://hivdb.stanford.edu). The database contains a compilation of nearly all published HIV RT and protea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.346
更新日期:2000-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1163
更新日期:2015-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf369
更新日期:2002-06-15 00:00:00
abstract::Telomeres at the ends of eukaryotic chromosomes are essential for genome integrality and stability. In order to identify genes that sustain telomere maintenance independently of telomerase recruitment, we have exploited the phenotype of over-long telomeres in the cells that express Cdc13-Est2 fusion protein, and exami...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1150
更新日期:2020-12-16 00:00:00
abstract::An investigation of the mobility of short duplexes of DNA -octamers and decamers-on polyacrylamide gels is presented, showing that molecules containing less than one helical turn exhibit sequence dependent mobilities. Analysis of chains with different sequences indicates that any arrangement of two or more adjacent A....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.14.6803
更新日期:1988-07-25 00:00:00
abstract::A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with its cognate and non cognate yeast tRNAs. It was shown that, when limited amounts of tRNA (either cognate or non cognate) a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.7.2421
更新日期:1977-07-01 00:00:00
abstract::Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuri...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1983-11-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.2.825
更新日期:1987-01-26 00:00:00
abstract::According to the recruitment model of transcriptional activation, an activator helps initiate transcription by bringing the RNA polymerase to a specific location on the DNA through interaction with components of the transcriptional machinery. However, it is difficult to isolate and define the activities of specific ac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1227
更新日期:2010-04-01 00:00:00
abstract::Nam8, a component of yeast U1 snRNP, is optional for mitotic growth but required during meiosis, because Nam8 collaborates with Mer1 to promote splicing of essential meiotic mRNAs AMA1, MER2 and MER3. Here, we identify SPO22 and PCH2 as novel targets of Nam8-dependent meiotic splicing. Whereas SPO22 splicing is co-dep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1328
更新日期:2011-04-01 00:00:00
abstract::Dlx homeobox genes are first expressed in embryonic retina at E11.5. The Dlx1/Dlx2 null retina has a reduced ganglion cell layer (GCL), with loss of late-born differentiated retinal ganglion cells (RGCs) due to increased apoptosis. TrkB signaling is proposed to regulate the dynamics of RGC apoptosis throughout develop...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1099
更新日期:2008-02-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) that have no protein-coding capacity make up a large proportion of the transcriptome of various species. Many lncRNAs are expressed within the animal central nervous system in spatial- and temporal-specific patterns, indicating that lncRNAs play important roles in cellular processes, neu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks943
更新日期:2012-12-01 00:00:00
abstract::Biological networks play increasingly important roles in omics data integration and systems biology. Over the past decade, many excellent tools have been developed to support creation, analysis and visualization of biological networks. However, important limitations remain: most tools are standalone programs, the majo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky510
更新日期:2018-07-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks056
更新日期:2012-05-01 00:00:00
abstract::Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered liga...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl176
更新日期:2006-04-28 00:00:00