Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.

abstract：:The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3'-->5' helicase and 3'-->5' exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functi...

journal_title：Nucleic acids research

authors： Karmakar P,Snowden CM,Ramsden DA,Bohr VA

更新日期：2002-08-15 00:00:00

abstract：:Mammalian antibody switch regions (∼1500 bp) are composed of a series of closely neighboring G4-capable sequences. Whereas numerous structural and genome-wide analyses of roles for minimal G4s in transcriptional regulation have been reported, Long G4-capable regions (LG4s)-like those at antibody switch regions-remain ...

journal_title：Nucleic acids research

authors： Williams JD,Houserova D,Johnson BR,Dyniewski B,Berroyer A,French H,Barchie AA,Bilbrey DD,Demeis JD,Ghee KR,Hughes AG,Kreitz NW,McInnis CH,Pudner SC,Reeves MN,Stahly AN,Turcu A,Watters BC,Daly GT,Langley RJ,Gillesp

更新日期：2020-06-19 00:00:00

abstract：:The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...

journal_title：Nucleic acids research

authors： Méjean V,Devedjian JC,Rives I,Alloing G,Claverys JP

更新日期：1991-10-25 00:00:00

abstract：:We describe the further development of a widely used package of DNA/protein sequence analysis programs (1). Important revisions have been made based on user experience, and new features, multi-user capability, and a set of large scale homology programs have been added. The programs are very user friendly, economical o...

journal_title：Nucleic acids research

authors： Pustell J,Kafatos FC

更新日期：1984-01-11 00:00:00

abstract：:The webPDBinder (http://pdbinder.bio.uniroma2.it/PDBinder) is a web server for the identification of small ligand-binding sites in a protein structure. webPDBinder searches a protein structure against a library of known binding sites and a collection of control non-binding pockets. The number of similarities identifie...

journal_title：Nucleic acids research

authors： Bianchi V,Mangone I,Ferrè F,Helmer-Citterich M,Ausiello G

更新日期：2013-07-01 00:00:00

abstract：:For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a heterologous mammalian ge...

journal_title：Nucleic acids research

authors： Adachi N,Ikeda H,Kikuchi A

更新日期：1994-10-11 00:00:00

abstract：:A mutant at the carboxyl end of the terminal protein, p3, of phage phi 29 DNA has been constructed by inserting an containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a phage phi 29 DNA fragment coding for all but the last five amino acids of protein p3. The activ...

journal_title：Nucleic acids research

authors： Mellado RP,Salas M

更新日期：1983-11-11 00:00:00

abstract：:The behaviour of highly purified bacterial expressed rat O6-methylguanine-DNA methyltransferase (MGMT) towards the repair of CGCm6GAGCTCGCG and CGCe6GAGCTCGCG (km6G/ke6G = 1.45, where k is the second order repair rate constant determined, m6G and e6G are O6-methyl and O6-ethylguanine) is similar to that of E. coli 39k...

journal_title：Nucleic acids research

authors： Liem LK,Lim A,Li BF

更新日期：1994-05-11 00:00:00

abstract：:A computer program, BRUCE, was developed for the identification of transfer-messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNA(Ala)-like secondary structure surrounding a short sequence encoding the tag peptide. In the 57 completely sequenced bacterial genomes where tmRNA genes ...

journal_title：Nucleic acids research

authors： Laslett D,Canback B,Andersson S

更新日期：2002-08-01 00:00:00

abstract：:Messenger RNAs homologous to a cDNA clone (pTOM 13) derived from a ripe-tomato-specific cDNA library are expressed during tomato fruit ripening and after the wounding of leaf and green fruit material. Both responses involve the synthesis of the hormone ethylene. Accumulation of the pTOM 13--homologous RNA during ripen...

journal_title：Nucleic acids research

authors： Holdsworth MJ,Bird CR,Ray J,Schuch W,Grierson D

更新日期：1987-01-26 00:00:00

abstract：:The tight-binding I12-X86 lac repressor binds to non-operator DNA in a sequence-specific fashion. Using the DNA of the E. coli I gene we have investigated these sequence-specific interactions and compared them to the operator binding of wild-type repressor. The specific, non-operator DNA interactions are sensitive to ...

journal_title：Nucleic acids research

authors： Schmitz A,Galas DJ

更新日期：1980-02-11 00:00:00

abstract：:The sigma(54) factor associates with core RNA polymerase (RNAP) to form a holoenzyme that is unable to initiate transcription unless acted on by an activator protein. sigma(54) is closely involved in many steps of activator-dependent transcription, such as core RNAP binding, promoter recognition, activator interaction...

journal_title：Nucleic acids research

authors： Xiao Y,Wigneshweraraj SR,Weinzierl R,Wang YP,Buck M

更新日期：2009-07-01 00:00:00

abstract：:As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...

journal_title：Nucleic acids research

authors： Yoshimura K,Rosenfeld MA,Nakamura H,Scherer EM,Pavirani A,Lecocq JP,Crystal RG

更新日期：1992-06-25 00:00:00

abstract：:The control of messenger RNA (mRNA) function by micro RNAs (miRNAs) in animal cells requires the GW182 protein. GW182 is recruited to the miRNA repression complex via interaction with Argonaute protein, and functions downstream to repress protein synthesis. Interaction with Argonaute is mediated by GW/WG repeats, whic...

journal_title：Nucleic acids research

authors： Chekulaeva M,Parker R,Filipowicz W

更新日期：2010-10-01 00:00:00

abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...

journal_title：Nucleic acids research

authors： Liu R,Iadevaia V,Averous J,Taylor PM,Zhang Z,Proud CG

更新日期：2014-04-01 00:00:00

abstract：:Nucleic acids possess the unique property of being enzymatically amplifiable, and have therefore been a popular choice for the combinatorial selection of functional sequences, such as aptamers or ribozymes. However, amplification typically requires known sequence segments that serve as primer binding sites, which can ...

journal_title：Nucleic acids research

authors： Wiesmayr A,Fournier P,Jäschke A

更新日期：2012-05-01 00:00:00

abstract：:The Gene Expression Atlas (http://www.ebi.ac.uk/gxa) is an added-value database providing information about gene expression in different cell types, organism parts, developmental stages, disease states, sample treatments and other biological/experimental conditions. The content of this database derives from curation, ...

journal_title：Nucleic acids research

authors： Kapushesky M,Emam I,Holloway E,Kurnosov P,Zorin A,Malone J,Rustici G,Williams E,Parkinson H,Brazma A

更新日期：2010-01-01 00:00:00

abstract：:Potential pathways that could account for observed rapid rates of transcription reinitiation were explored. A nuclear extract system was established in which reinitiation rates were observed to be kinetically facilitated and in which the rate was sensitive to TATA box mutation. Kinetic facilitation of functional compl...

journal_title：Nucleic acids research

authors： Yean D,Gralla JD

更新日期：1999-02-01 00:00:00

abstract：:Algae are a diverse, polyphyletic group of photosynthetic eukaryotes spanning nearly all eukaryotic lineages of life and collectively responsible for ∼50% of photosynthesis on Earth. Sequenced algal genomes, critical to understanding their complex biology, are growing in number and require efficient tools for analysis...

journal_title：Nucleic acids research

authors： Grigoriev IV,Hayes RD,Calhoun S,Kamel B,Wang A,Ahrendt S,Dusheyko S,Nikitin R,Mondo SJ,Salamov A,Shabalov I,Kuo A

更新日期：2021-01-08 00:00:00

abstract：:This laboratory has established in previous studies that Pmel 17, a gene expressed specifically in melanocytes, maps near the silver coat color locus (si/si) on mouse chromosome 10. In the current study, we have focused on determining whether or not the si allele carries a mutation in Pmel 17. Pmel 17 cDNA clones, iso...

journal_title：Nucleic acids research

authors： Kwon BS,Halaban R,Ponnazhagan S,Kim K,Chintamaneni C,Bennett D,Pickard RT

更新日期：1995-01-11 00:00:00

abstract：:Targeted differentiation of human induced pluripotent stem cells (hiPSCs) using only chemicals would have value-added clinical potential in the regeneration of complex cell types including cardiomyocytes. Despite the availability of several chemical inhibitors targeting proteins involved in signaling pathways, no bioa...

journal_title：Nucleic acids research

authors： Taniguchi J,Pandian GN,Hidaka T,Hashiya K,Bando T,Kim KK,Sugiyama H

更新日期：2017-09-19 00:00:00

abstract：:Mitochondrial DNA (mtDNA) resides in a high ROS environment and suffers more mutations than its nuclear counterpart. Increasing evidence suggests that mtDNA mutations are not the results of direct oxidative damage, rather are caused, at least in part, by DNA replication errors. To understand how the mtDNA replicase, P...

journal_title：Nucleic acids research

authors： Anderson AP,Luo X,Russell W,Yin YW

更新日期：2020-01-24 00:00:00

abstract：:By successively assembling genetic parts such as BioBrick according to grammatical models, complex genetic constructs composed of dozens of functional blocks can be built. However, usually every category of genetic parts includes a few or many parts. With increasing quantity of genetic parts, the process of assembling...

journal_title：Nucleic acids research

authors： Fang G,Zhang S,Dong Y

更新日期：2017-12-15 00:00:00

abstract：:We have sequenced the 0.7-kb-long fragment of Drosophila DNA which ensures the autonomous replication of plasmids in yeast. Deletion mapping has shown the ARS element to consist of at least two domains: the core having the consensus 11-bp sequence TAAATATAAAT and the enhancer which is no more than 90 bp long and is lo...

journal_title：Nucleic acids research

authors： Gragerov AI,Danilevskaya ON,Didichenko SA,Kaverina EN

更新日期：1988-02-11 00:00:00

abstract：:This paper describes a method for predicting a supersecondary structural motif, beta-hairpins, in a protein sequence. The method was trained and tested on a set of 5102 hairpins and 5131 non-hairpins, obtained from a non-redundant dataset of 2880 proteins using the DSSP and PROMOTIF programs. Two machine-learning tech...

journal_title：Nucleic acids research

authors： Kumar M,Bhasin M,Natt NK,Raghava GP

更新日期：2005-07-01 00:00:00

abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...

journal_title：Nucleic acids research

authors： Wang XC,Wilson SC,Hammond MC

更新日期：2016-09-30 00:00:00

abstract：:Sequence-specific binding of proteins to their DNA targets involves a complex spectrum of processes that often induce DNA conformational variation in the bound complex. The forces imposed by protein binding that cause the helical deformations are intimately interrelated and difficult to parse or rank in importance. To...

journal_title：Nucleic acids research

authors： Grant BN,Dourlain EM,Araneda JN,Throneberry ML,McFail-Isom LA

更新日期：2013-08-01 00:00:00

abstract：:Reversion of T type cytoplasmic male sterility (cmsT) to fertility is correlated with sequence changes in a 1.5 kb AvaI fragment. This 1.5 kb AvaI fragment is composed of 5' flanking sequences of ATPase subunit 6, one complete open reading frame (ORF 13) and part of the another (ORF 25). The sequence of the 1.5 kb Ava...

journal_title：Nucleic acids research

authors： Qin J,Fauron CM,Brettell RI,Milhous M,Abbott AG

更新日期：1987-08-11 00:00:00

abstract：:A flow-injection (FI) device is combined, through the use of a low-volume (4 microl) flow cell, with an ultrasensitive surface plasmon resonance (SPR) spectrometer equipped with a bi-cell photodiode detector. The application of this novel FI-SPR device for sequence-specific ultratrace analysis of oligodeoxynucleotides...

journal_title：Nucleic acids research

authors： Song F,Zhou F,Wang J,Tao N,Lin J,Vellanoweth RL,Morquecho Y,Wheeler-Laidman J

更新日期：2002-07-15 00:00:00

abstract：:The Bovine Genome Database (BGD; http://BovineGenome.org) strives to improve annotation of the bovine genome and to integrate the genome sequence with other genomics data. BGD includes GBrowse genome browsers, the Apollo Annotation Editor, a quantitative trait loci (QTL) viewer, BLAST databases and gene pages. Genome ...

journal_title：Nucleic acids research

authors： Childers CP,Reese JT,Sundaram JP,Vile DC,Dickens CM,Childs KL,Salih H,Bennett AK,Hagen DE,Adelson DL,Elsik CG

更新日期：2011-01-01 00:00:00