Abstract：
:Ovarian tissue cryopreservation is considered as a useful option to preserve fertility for cancer patients. This study purposed to evaluate the change of telomere length and senescence markers during human ovarian tissue cryopreservation. Ovarian tissues were obtained from women who underwent benign ovarian surgery in the gynecology research unit of a university hospital with prior consent and IRB approval. DNA was extracted from the ovarian tissues using a DNeasy tissue kit and telomere lengths in the DNA samples were determined by real time PCR before and after cryopreservation. All tissues were stained with hematoxylin-eosin and subjected to immunohistochemistry and TUNEL assays. Other senescence markers, including p53, p16, p21, and phospho-pRb proteins, were evaluated using western blot analysis. Ovarian tissues were collected from ten patients and prepared for slow freezing with the same size of diameter 4 mm and 1 mm thickness. Mean age of patients was 26.7 ± 6.2 years (range, 16-34 years), and ovarian tissues were cryopreserved and thawed 4 weeks after cryopreservation. The mean telomere length was significantly decreased after cryopreservation (9.57 ± 1.47 bp vs. 8.34 ± 1.83 bp, p = 0.001). Western blot analysis revealed that p53, p16, and p21 proteins increased and phospho-pRb protein expression decreased after ovarian tissue cryopreservation. Ovarian tissue cryopreservation and transplantation is regarded as one of promising options for fertility preservation. However, clinicians and researchers should be aware of possible irreversible DNA changes such as shortening of telomere length and alterations of other senescence markers in human ovarian tissues.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kim B,Ryu KJ,Lee S,Kim Tdoi
10.1038/s41598-021-81973-3subject
Has Abstractpub_date
2021-01-26 00:00:00pages
2238issue
1issn
2045-2322pii
10.1038/s41598-021-81973-3journal_volume
11pub_type
杂志文章abstract：:By moving the alkoxy group on the spacer from position 5 to position 4 to add another protocol for the algorithm for intermolecular and intramolecular hydrogen bonding interactions, highly regioselectivity of noncovalent synthesis of the hydrazide-based cyclic hexamers was achieved: one out of thirteen possible isomer...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01059
更新日期：2013-01-01 00:00:00
abstract：:Aditoprim (ADP) is a newly developed antibacterial agent in veterinary medicine. The metabolism and disposition of ADP in swine, broilers, carp and rats were investigated by using a radio tracer method combined with a radioactivity detector and a liquid chromatography/ion trap/time-of-flight mass spectrometry. After a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20370
更新日期：2016-02-03 00:00:00
abstract：:Experts have the superior cognitive capability of quickly understanding complex information in their domain; however, little is known about the neural processes underlying this ability. Here, using a board game named shogi (Japanese chess), we investigated the brain activity in expert players that was involved in thei...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05894
更新日期：2014-07-31 00:00:00
abstract：:Ethoxyquin was recently identified as a neuroprotective compound against toxic neuropathies and efficacy was demonstrated against paclitaxel-induced neurotoxicity in vivo. In this study we examined the efficacy of ethoxyquin in preventing neurotoxicity of cisplatin in rodent models of chemotherapy-induced peripheral n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28861
更新日期：2016-06-28 00:00:00
abstract：:The human foot and ankle system is equipped with structures that can produce mechanical work through elastic (e.g., Achilles tendon, plantar fascia) or viscoelastic (e.g., heel pad) mechanisms, or by active muscle contractions. Yet, quantifying the work distribution among various subsections of the foot and ankle can ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15218-7
更新日期：2017-11-13 00:00:00
abstract：:Hurricanes are a persistent socio-economic hazard for countries situated in and around the Main Development Region (MDR) of Atlantic tropical cyclones. Climate-model simulations have attributed their interdecadal variability to changes in solar and volcanic activity, Saharan dust flux, anthropogenic greenhouse gas and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12838
更新日期：2015-08-05 00:00:00
abstract：:Spatially-resolved neutron powder diffraction with a gauge volume of 2 × 2 × 20 mm(3) has been applied as an in situ method to probe the lithium concentration in the graphite anode of different Li-ion cells of 18650-type in charged state. Structural studies performed in combination with electrochemical measurements an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18380
更新日期：2015-12-18 00:00:00
abstract：:Cartilage engineering that combines competent seeding cells and a compatible scaffold is increasingly gaining popularity and is potentially useful for the treatment of various bone and cartilage diseases. Intensive efforts have been made by researchers to improve the viability and functionality of seeding cells of eng...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26617
更新日期：2016-05-25 00:00:00
abstract：:To identify novel phenotypic associations related to Cytochrome P450 Family 2 Subfamily A Member 6 (CYP2A6), we investigated the human phenome in a total of 11,271 individuals. Initially, we conducted a phenome-wide association study in 3,401 nicotine-exposed elderly subjects considering 358 phenotypic traits. We iden...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01098-4
更新日期：2017-04-21 00:00:00
abstract：:Knockin (KI) mouse carrying a point mutation has been an invaluable tool for disease modeling and analysis. Genome editing technologies using the CRISPR/Cas system has emerged as an alternative way to create KI mice. However, if the mice carry nucleotide insertions and/or deletions (InDels) in other genes, which could...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34703
更新日期：2016-10-04 00:00:00
abstract：:Early treatment of single sided deafness in children has been recommended to protect from neurodevelopmental preference for the better hearing ear and from social and educational deficits. A fairly homogeneous group of five young children (≤3.6 years of age) with normal right sided hearing who received a cochlear impl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17129-z
更新日期：2017-12-04 00:00:00
abstract：:In this study, we examined the possible mechanisms of trehalose (Tre) in improving copper-stress (Cu-stress) tolerance in rice seedlings. Our findings indicated that pretreatment of rice seedlings with Tre enhanced the endogenous Tre level and significantly mitigated the toxic effects of excessive Cu on photosynthesis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11433
更新日期：2015-06-15 00:00:00
abstract：:The gliotransmitter glutamate released from astrocytes can modulate neuronal firing by activating neuronal N-methyl-D-aspartic acid (NMDA) receptors. This enables astrocytic glutamate(AG) to be involved in neuronal physiological and pathological functions. Based on empirical results and classical neuron-glial "tripart...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32343
更新日期：2016-08-30 00:00:00
abstract：:The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individual...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14925
更新日期：2015-10-08 00:00:00
abstract：:There is not a clear consensus regarding the optimal treatment of locally advanced pancreatic disease. There is a potential role for neoadjuvant therapy to treat micrometastatic disease with chemotherapy, as well as for the treatment of local disease with radiotherapy. We evaluated the safety and efficacy of induction...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45845
更新日期：2017-04-05 00:00:00
abstract：:Patients with advanced ovarian cancer develop recurrence despite initial treatment response to standard treatment of surgery and intravenous/intraperitoneal (IP) chemotherapy, partly due to a limited peritoneal exposure time of chemotherapeutics. Paclitaxel-loaded genipin-crosslinked gelatin microspheres (PTX-GP-MS) a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51419-y
更新日期：2019-10-16 00:00:00
abstract：:Inorganic elements are important components of medicinal herbs, and provide valuable experimental evidence for the quality evaluation and control of traditional Chinese medicine (TCM). In this study, to investigate the relationship between the inorganic elemental fingerprint and geographical origin identification of c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12933-z
更新日期：2017-10-03 00:00:00
abstract：:An ultra-fast physical random number generator is demonstrated utilizing a photonic integrated device based broadband chaotic source with a simple post data processing method. The compact chaotic source is implemented by using a monolithic integrated dual-mode amplified feedback laser (AFL) with self-injection, where ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45900
更新日期：2017-04-04 00:00:00
abstract：:Local and global changes associated with anthropogenic activities are impacting marine and terrestrial ecosystems. Macroalgae, especially habitat-forming species like kelp, play critical roles in temperate coastal ecosystems. However, their abundance and distribution patterns have been negatively affected by warming i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60104-4
更新日期：2020-02-21 00:00:00
abstract：:The study aimed to explore the epidemiology and clinical characteristics of chronic osteomyelitis observed in a northern China hospital. Clinical data of 255 patients with chronic osteomyelitis from January 2007 to January 2014 were collected and analyzed, including general information, disease data, treatment and fol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33106-6
更新日期：2018-10-05 00:00:00
abstract：:Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59988-z
更新日期：2020-02-20 00:00:00
abstract：:To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary for regulation of gene technology, an efficient method with high specificity and rapidity was developed for the cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set of four primers was designed usin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04912
更新日期：2014-05-09 00:00:00
abstract：:Dysregulation in acetylation homeostasis has been implicated in the pathogenesis of the amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disorder. It is known that the acetylation of transcriptional factors regulates their activity. The acetylation state of NF-kB RelA has been found to dictate the neurop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30659-4
更新日期：2018-08-27 00:00:00
abstract：:Nuclear magnetic resonance (NMR) is a fundamental research tool that is widely used in many fields. Despite its powerful applications, unfortunately the low sensitivity of conventional NMR makes it difficult to study thin film or nano-sized samples. In this work, we report the first NMR spectrum obtained from general ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03189
更新日期：2013-11-12 00:00:00
abstract：:Structural isomers, molecules having the same chemical formula but with atoms bonded in different order, are hard to identify using conventional spectroscopy and mass spectrometry. They exhibit virtually indistinguishable mass spectra when ionized by electrons. Laser mass spectrometry based on photoionization of the i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22055-9
更新日期：2018-02-28 00:00:00
abstract：:Wss1 is a DNA-protein crosslinks (DPCs) repair protein, which is responsible for degradation of the protein components in DPCs. In this investigation, crystal structure of the protease domain from saccharomyces cerevisiae Wss1 (ScWss1) was solved and was compared with the known crystal structure of Schizosaccharomyces...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08834-w
更新日期：2017-08-15 00:00:00
abstract：:Ten to twenty percent of the hepatocellular carcinoma (HCC) patients fulfilling the Milan criteria (MC) recurred within three years after orthotopic liver transplantation (OLT). We therefore utilize a training cohort to develop an improved prognostic model for predicting the recurrence in these patients. By univariate...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep44036
更新日期：2017-03-09 00:00:00
abstract：:Replacing precious and nondurable Pt catalysts with cheap materials is a key issue for commercialization of fuel cells. In the case of oxygen reduction reaction (ORR) catalysts for direct methanol fuel cell (DMFC), the methanol tolerance is also an important concern. Here, we develop AlN nanowires with diameters of ab...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06013
更新日期：2014-08-11 00:00:00
abstract：:We investigated in this paper a novel bilayer composite obtained by sol-gel and spin coating of the ferroelectric 0.92Na0.5Bi0.5TiO3-0.08BaTiO3 (abbreviated as BNT-BT0.08) and ferromagnetic CoFe2O4 phases, for miniature low-frequency magnetic sensors and piezoelectric sensors. This heterostructure, deposited on Si-Pt ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36232-3
更新日期：2018-12-14 00:00:00
abstract：:After the accident at the Fukushima Daiichi Nuclear Power Plant run by Tokyo Electric Power Company in 2011, breast milk samples obtained from volunteers living in Fukushima and neighboring prefectures were examined and small amounts of I-131 (2.2-36.3 Bq/kg) were detected in some samples. In this work, the I-131 conc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12426
更新日期：2015-07-22 00:00:00
