Abstract:
:GenBank® (https://www.ncbi.nlm.nih.gov/genbank/) is a comprehensive, public database that contains 9.9 trillion base pairs from over 2.1 billion nucleotide sequences for 478 000 formally described species. Daily data exchange with the European Nucleotide Archive and the DNA Data Bank of Japan ensures worldwide coverage. Recent updates include new resources for data from the SARS-CoV-2 virus, updates to the NCBI Submission Portal and associated submission wizards for dengue and SARS-CoV-2 viruses, new taxonomy queries for viruses and prokaryotes, and simplified submission processes for EST and GSS sequences.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sayers EW,Cavanaugh M,Clark K,Pruitt KD,Schoch CL,Sherry ST,Karsch-Mizrachi Idoi
10.1093/nar/gkaa1023subject
Has Abstractpub_date
2021-01-08 00:00:00pages
D92-D96issue
D1eissn
0305-1048issn
1362-4962pii
5983623journal_volume
49pub_type
杂志文章abstract::Two enzymatic pathways are involved in the inhibitory effects of double-stranded (ds)RNA on protein synthesis in cell extracts derived from interferon-treated human fibroblasts or HeLa cells, an oligonucleotide polymerase that synthesizes (2'-5')An from ATP and a protein kinase that phosphorylates the alpha subunit of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.19.4939
更新日期:1981-10-10 00:00:00
abstract::Repression of cellular reprogramming in germ cells is critical to maintaining cell fate and fertility. When germ cells mis-express somatic genes they can be directly converted into other cell types, resulting in loss of totipotency and reproductive potential. Identifying the molecular mechanisms that coordinate these ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa174
更新日期:2020-05-07 00:00:00
abstract::The accuracy of a homology model based on the structure of a distant relative or other topologically equivalent protein is primarily limited by the quality of the alignment. Here we describe a systematic approach for sequence-to-structure alignment, called 'K*Sync', in which alignments are generated by dynamic program...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl480
更新日期:2006-01-01 00:00:00
abstract::Activation of the transcription factor NF-kappaB is critical for the tumor necrosis factor-alpha (TNF-alpha)-induced inflammatory response. Here we report the complete gene expression profile from activated microvascular endothelial cells emphasizing the direct contribution of the NF-kappaB pathway. Human microvascula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki836
更新日期:2005-09-21 00:00:00
abstract::PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.22.10669
更新日期:1988-11-25 00:00:00
abstract::NRPS-PKS is web-based software for analysing large multi-enzymatic, multi-domain megasynthases that are involved in the biosynthesis of pharmaceutically important natural products such as cyclosporin, rifamycin and erythromycin. NRPS-PKS has been developed based on a comprehensive analysis of the sequence and structur...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh359
更新日期:2004-07-01 00:00:00
abstract::Bacterial RNase III plays important roles in the processing and degradation of RNA transcripts. A major goal is to identify the cleavage targets of this endoribonuclease at a transcriptome-wide scale and delineate its in vivo cleavage rules. Here we applied to Escherichia coli grown to either exponential or stationary...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky684
更新日期:2018-11-02 00:00:00
abstract::High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1210
更新日期:2016-03-18 00:00:00
abstract::The TWIST1 bHLH transcription factor controls embryonic development and cancer processes. Although molecular and genetic analyses have provided a wealth of data on the role of bHLH transcription factors, very little is known on the molecular mechanisms underlying their binding affinity to the E-box sequence of the pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw334
更新日期:2016-06-20 00:00:00
abstract::A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.19.4395
更新日期:1998-10-01 00:00:00
abstract::LINE1s occupy 17% of the human genome and are its only active autonomous mobile DNA. L1s are also responsible for genomic insertion of processed pseudogenes and >1 million non-autonomous retrotransposons (Alus and SVAs). These elements have significant effects on gene organization and expression. Despite the importanc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt512
更新日期:2013-08-01 00:00:00
abstract::The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1344
更新日期:2011-05-01 00:00:00
abstract::Not I linking clones contain sequences flanking Not I recognition sites and were previously shown to be tightly associated with CpG islands and genes. To directly assess the value of Not I clones in genome research, high density grids with 50 000 Not I linking clones originating from six representative Not I linking l...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.7.1635
更新日期:2000-04-01 00:00:00
abstract::We report here the discovery of HMBP, a protein in nuclei of human T-helper lymphocytes and other human cell types, which binds with enhanced affinity to a promoter element in the HIV-1 long terminal repeat when that element is methylated at CpGs, the target site of the human DNA methyltransferase. This promoter eleme...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.24.5786
更新日期:1993-12-11 00:00:00
abstract::An endo-exonuclease has been purified from cultured monkey (CV-1) cells. The enzyme which was purified to near homogeneity to be a 65 kDa monomeric protein. The single-strand DNase activity is endonucleolytic and nonprocessive, whereas the double-strand DNase activity is exonucleolytic and processive. The enzyme was a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.16.4355
更新日期:1992-08-25 00:00:00
abstract::DNA methylation is the best-studied epigenetic modification and describes the conversion of cytosine to 5-methylcytosine. The importance of this phenomenon is that aberrant promoter hypermethylation is a common occurrence in cancer and is frequently associated with gene silencing. Various techniques are currently avai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnj017
更新日期:2006-02-07 00:00:00
abstract::Adaptation of eucaryotic cDNA to heterologous expression was studied by mutating the translation initiation (TI) region upstream (mTI) and downstream (MTI) of the start codon. In the mTI subregion the 8 bases flanking the invariant Shine-Dalgarno motif GG-AG were mutagenized exhaustively, while the MTI subregion was s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.12.3127
更新日期:1992-06-25 00:00:00
abstract::The U1 snRNP-specific 70K protein is one of the few snRNP proteins from higher eukaryotic cells that is phosphorylated in vivo (1,2). Immunoaffinity purified spliceosomal snRNPs (U1, U2, U5, and U4/U6) were tested for their ability to phosphorylate in vitro the U1-specific 70K protein. An snRNP-associated kinase activ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.12.2815
更新日期:1993-06-25 00:00:00
abstract::A recently reported cDNA clone coding for human promyelocytic L apoferritin, shows some differences with a liver L apoferritin cDNA. We have investigated if these differences are due to the expression of different genes or to an alternative transcription of an unique gene. In this paper we report data suggesting that ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.7.2863
更新日期:1986-04-11 00:00:00
abstract::Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.3.915
更新日期:1979-03-01 00:00:00
abstract::Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.11.2077
更新日期:1975-11-01 00:00:00
abstract::Ribonuclease MRP is an endonuclease, related to RNase P, which functions in eukaryotic pre-rRNA processing. In Saccharomyces cerevisiae, RNase MRP comprises an RNA subunit and ten proteins. To improve our understanding of subunit roles and enzyme architecture, we have examined protein-protein and protein-RNA interacti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm553
更新日期:2007-01-01 00:00:00
abstract::Alternative first exons diversify the transcriptomes of eukaryotes by producing variants of the 5' Untranslated Regions (5'UTRs) and N-terminal coding sequences. Accurate transcriptome-wide detection of alternative first exons typically requires specialized experimental approaches that are designed to identify the 5' ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky053
更新日期:2018-05-04 00:00:00
abstract::The DNA-binding specificity and affinity of the dimeric human transcription factor (TF) STAT1, were assessed by total internal reflectance fluorescence protein-binding microarrays (TIRF-PBM) to evaluate the effects of protein phosphorylation, higher-order polymerization and small-molecule inhibition. Active, phosphory...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1085
更新日期:2013-01-01 00:00:00
abstract::Homologous recombination is essential for the preservation of genome stability, thereby preventing cancer. The recombination protein RAD51 drives DNA strand exchange, which requires the assembly, rearrangement and disassembly of a RAD51 filament on DNA, coupled to ATP binding and hydrolysis. This process is facilitate...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr295
更新日期:2011-08-01 00:00:00
abstract::In the past year, DDBJ (http://www.ddbj.nig.ac.jp) collected and released 1,956,826 entries or 1,741,313,111 bases. The released data include approximately 90,000 ESTs and cDNAs of Macaca fascicularis, and 280 million bases of mouse GSS. In addition to the data collection, we have indexed the submitted data to the Int...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj111
更新日期:2006-01-01 00:00:00
abstract::Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn624
更新日期:2008-11-01 00:00:00
abstract::The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenoty...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.1.166
更新日期:1996-01-01 00:00:00
abstract::In most bacteria, two tRNAs decode the four arginine CGN codons. One tRNA harboring a wobble inosine (tRNA(Arg)ICG) reads the CGU, CGC and CGA codons, whereas a second tRNA harboring a wobble cytidine (tRNA(Arg)CCG) reads the remaining CGG codon. The reduced genomes of Mycoplasmas and other Mollicutes lack the gene en...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt356
更新日期:2013-07-01 00:00:00
abstract::Two models have been proposed for triggering release of the lagging strand polymerase at the replication fork, enabling cycling to the primer for the next Okazaki fragment--either collision with the 5'-end of the preceding fragment (collision model) or synthesis of a new primer by primase (signaling model). Specific p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1098
更新日期:2014-02-01 00:00:00