Abstract:
:Melatonin, a pineal gland secretion, is an amphiphilic neurohormone involved in the biological and physiologic regulation of bodily functions. Numerous studies have shown the effects of melatonin on the release of gonadotropins and their actions at one or several levels of the hypothalamic-pituitary-gonadal axis. However, direct melatonin action on gonadotropin-releasing hormone (GnRH) neurons and its mechanism of action remain unclear. Here, plasma melatonin levels were measured and the effect of melatonin on GnRH neurons was assessed using brain slice patch clamp techniques. The plasma melatonin levels in prepubertal mice were higher than those in the adults. Melatonin itself did not change the firing activity of GnRH neurons. Interestingly, the kainate receptor-mediated responses but not the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)- and N-methyl-D-aspartic acid (NMDA)-induced responses were suppressed by melatonin in both the voltage clamp and current clamp modes. The inhibitory effects of the kainate-induced response by melatonin tended to increase with higher melatonin concentrations and persisted in the presence of tetrodotoxin, a voltage-sensitive Na+ channel blocker, or luzindole, a non-selective melatonin receptor antagonist. However, the response was completely abolished by pretreatment with pertussis toxin. These results suggest that melatonin can regulate GnRH neuronal activities in prepubertal mice by partially suppressing the excitatory signaling mediated by kainate receptors through pertussis toxin-sensitive G-protein-coupled receptors.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Rijal S,Cho DH,Park SA,Jang SH,Ábrahám IM,Han SKdoi
10.3390/ijms21175991subject
Has Abstractpub_date
2020-08-20 00:00:00issue
17issn
1422-0067pii
ijms21175991journal_volume
21pub_type
杂志文章abstract::The human cytochrome P450 (CYP) superfamily consisting of 57 functional genes is the most important group of Phase I drug metabolizing enzymes that oxidize a large number of xenobiotics and endogenous compounds, including therapeutic drugs and environmental toxicants. The CYP superfamily has been shown to expand itsel...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17071020
更新日期:2016-06-28 00:00:00
abstract::DNA G-quadruplexes (G4s) form in relevant genomic regions and intervene in several biological processes, including the modulation of oncogenes expression, and are potential anticancer drug targets. The human KRAS proto-oncogene promoter region contains guanine-rich sequences able to fold into G4 structures. Here, by u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010448
更新日期:2021-01-05 00:00:00
abstract::Over the past few decades, metal nanoparticles less than 100 nm in diameter have made a substantial impact across diverse biomedical applications, such as diagnostic and medical devices, for personalized healthcare practice. In particular, silver nanoparticles (AgNPs) have great potential in a broad range of applicati...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20040865
更新日期:2019-02-17 00:00:00
abstract::Insulin-resistance is one of the main factors responsible for the onset and progression of Metabolic Syndrome (MetS). Among all polyphenols, the effects of flavonoids and their main food sources on insulin sensitivity have been widely evaluated in molecular and clinical studies. The aim of this review is to analyse th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20092061
更新日期:2019-04-26 00:00:00
abstract::Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms161024918
更新日期:2015-10-20 00:00:00
abstract::The presence of calcium deposits in human lesions is largely used as imaging biomarkers of human diseases such as breast cancer. Indeed, the presence of micro- or macrocalcifications is frequently associated with the development of both benign and malignant lesions. Nevertheless, the molecular mechanisms involved in t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22010120
更新日期:2020-12-24 00:00:00
abstract::Estrogen receptor-α positive (ERα⁺) breast cancers represent 75% of all invasive breast cancer cases, while de novo or acquired resistance to ER-directed therapy is also on the rise. Numerous factors contribute to this phenomenon including the recently-reported ESR1 gene mutations such as Y537S, which amplifies co-act...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020579
更新日期:2018-02-15 00:00:00
abstract::Tanshinone IIA (Tan-IIA) is an extract from the widely used traditional Chinese medicine (TCM) Danshen (Salvia miltiorrhiza), and has been found to attenuate the proliferation of bladder cancer (BCa) cells (The IC50 were: 5637, 2.6 μg/mL; BFTC, 2 μg/mL; T24, 2.7 μg/mL, respectively.). However, the mechanism of the eff...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081616
更新日期:2017-07-25 00:00:00
abstract::We examined the effects of gut microbial catabolites of tryptophan on the aryl hydrocarbon receptor (AhR). Using a reporter gene assay, we show that all studied catabolites are low-potency agonists of human AhR. The efficacy of catabolites differed substantially, comprising agonists with no or low (i3-propionate, i3-a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072614
更新日期:2020-04-09 00:00:00
abstract::Topical or systemic administration of JAK inhibitors has been shown to be a new treatment modality for severe alopecia areata (AA). Some patients show a good response to JAK inhibitors, but frequently relapse after cessation of the treatment. There have been no guidelines about the indications and use of JAK inhibitor...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21145137
更新日期:2020-07-20 00:00:00
abstract::Antibodies are composed of structurally and functionally independent domains that can be used as building blocks to construct different types of chimeric protein-format molecules. However, the generally used genetic fusion and chemical approaches restrict the types of structures that can be formed and do not give an i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030711
更新日期:2020-01-21 00:00:00
abstract::The surface attachment properties of the Creutz-Taube ion, i.e., [(NH(3))(5)Ru(pyrazine)Ru(NH(3))(5)](5+), on both hydrophilic and hydrophobic types of surfaces were investigated using X-ray photoelectron spectroscopy (XPS). The results indicated that the Creutz-Taube ions only bound to hydrophilic surfaces, such as S...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10020533
更新日期:2009-02-01 00:00:00
abstract::Unfolded protein response (UPR) is a cytoprotective mechanism that alleviates the protein-folding burden in eukaryotic organisms. Moderate activation of UPR is required for maintaining endoplasmic reticulum (ER) homeostasis and profoundly contributes to tumorigenesis. Defects in UPR signaling are implicated in the att...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19071832
更新日期:2018-06-21 00:00:00
abstract::Patients suffering from liver cirrhosis are often complicated with the formation of portosystemic collateral vessels, which is associated with the progression of a splanchnic hyperdynamic circulatory state. Alleviating pathological angiogenesis has thus been proposed to be a feasible treatment strategy. Indole-3-carbi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20174161
更新日期:2019-08-26 00:00:00
abstract::Citral is well known for its antimicrobial, antifungal, and insecticidal activities. Natural sesquiterpene α-methylenelactones also exhibit a broad spectrum of biological activities. The aim of the study was to explore the effect of structural changes to citral molecules on citral behavior-modifying activity towards M...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218029
更新日期:2020-10-28 00:00:00
abstract::Osteosarcoma therapy might be moving toward nanotechnology-based drug delivery systems to reduce the cytotoxicity of antineoplastic drugs and improve their pharmacokinetics. In this paper, we present, for the first time, an extensive chemical and in vitro characterization of dual-loaded photo- and chemo-active keratin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113670
更新日期:2018-11-20 00:00:00
abstract::In order to supply new information on the antioxidant function of selected beverages for nutritionists and the general public, total phenolic contents of 51 kinds of herbal and tea infusions made in China were measured by the Folin-Ciocalteu method, and their antioxidant capacities were evaluated using ferric reducing...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12042112
更新日期:2011-01-01 00:00:00
abstract::Adenosine is a neuromodulator that has been involved in aging and neurodegenerative diseases as Alzheimer's disease (AD). In the present work, we analyzed the possible modulation of purine metabolites, 5'nucleotidase (5'NT) and adenosine deaminase (ADA) activities, and adenosine monophosphate (AMP)-activated protein k...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197300
更新日期:2020-10-02 00:00:00
abstract::Our previous study showed that a single lipopolysaccharide (LPS) treatment to neonatal rats could induce a long-lasting neuroinflammatory response and dopaminergic system injury late in life. This is evidenced by a sustained activation of microglia and elevated interleukin-1β (IL-1β) levels, as well as reduced tyrosin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16048635
更新日期:2015-04-17 00:00:00
abstract::West Nile virus (WNV), a mosquito-borne arbovirus, remains a major global health concern. In this study, we optimized PCR methods then assessed serially-collected whole blood (WB), urine (UR), saliva, and semen specimens from a large cohort of WNV-positive participants to evaluate the natural history of infection and ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20081934
更新日期:2019-04-19 00:00:00
abstract::Recent studies have shown that free fatty acids are associated with chronic inflammation, which may be involved in vascular injury. The intake of eicosapentaenoic acid (EPA) can decrease cardiovascular disease risks, but the protective mechanisms of EPA on endothelial cells remain unclear. In this study, primary human...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150610334
更新日期:2014-06-10 00:00:00
abstract::Salmonella typhimurium cells were subjected to pulsed electric field (PEF) treatment at 25 kV/cm for 0-4 ms to investigate the effect of PEF on the cytoplasmic membrane lipids and oxidative injury of cells. Results indicated that PEF treatment induced a decrease of membrane fluidity of Salmonella typhimurium (S. typhi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081374
更新日期:2016-08-22 00:00:00
abstract::Esterification of starch was carried out to expand the usefulness of starch for a myriad of industrial applications. Lipase B from Candida antarctica, immobilized on macroporous acrylic resin (Novozym 435), was used for starch esterification in two reaction systems: micro-solvent system and solvent-free system. The es...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13067226
更新日期:2012-01-01 00:00:00
abstract::Plants have been interacting with insects for several hundred million years, leading to complex defense approaches against various insect feeding strategies. Some defenses are constitutive while others are induced, although the insecticidal defense compound or protein classes are often similar. Insect herbivory induce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140510242
更新日期:2013-05-16 00:00:00
abstract::Several studies have demonstrated that basic fibroblast growth factor (bFGF) can induce neural differentiation of mesenchymal stem cells. In this study, we investigated the neural differentiation of muscle-derived stem cells (MDSCs) following treatment with bFGF and ethosuximide, a small molecule used as an anticonvul...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14046614
更新日期:2013-03-25 00:00:00
abstract::Benzimidazole derivatives have a diverse range of biological activities, including antiulcer, antihypertensive, antiviral, antifungal, anti-inflammatory, and anticancer. Despite these activities, previous studies have revealed that some of the derivatives can induce mutations. This study aimed to screen for potential ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184324
更新日期:2019-09-04 00:00:00
abstract::Breakthroughs in the development of programmable site-specific nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases (MNs), and most recently, the clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (including Cas9)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122565
更新日期:2017-11-29 00:00:00
abstract::Autoinflammatory diseases (AIDs) are heterogeneous disorders characterized by dysregulation in the inflammasome, a large intracellular multiprotein platform, leading to overproduction of interleukin-1(IL-1)β that plays a predominant pathogenic role in such diseases. Appropriate treatment is crucial, also considering t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20081898
更新日期:2019-04-17 00:00:00
abstract::Enzymatic activation of irinotecan (CPT-11) is due to carboxylesterase (CES), and its pharmacological behavior is influenced by drug resistance-related proteins. We previously reported that the clinical response and prognosis of metastatic colorectal cancer (mCRC) patients did not differ in tumors with different thymi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150915767
更新日期:2014-09-05 00:00:00
abstract::Mucopolysaccharidosis IVA (MPS IVA) is caused by a deficiency of the lysosomal enzyme N-acetylgalactosamine-6-sulfate sulfatase (GALNS). Conventional enzyme replacement therapy (ERT) is approved for MPS IVA. However, the fact that the infused enzyme cannot penetrate avascular lesions in cartilage leads to minimal impa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20174139
更新日期:2019-08-24 00:00:00