Abstract：
:Salmonella typhimurium cells were subjected to pulsed electric field (PEF) treatment at 25 kV/cm for 0-4 ms to investigate the effect of PEF on the cytoplasmic membrane lipids and oxidative injury of cells. Results indicated that PEF treatment induced a decrease of membrane fluidity of Salmonella typhimurium (S. typhimuriumi), possibly due to the alterations of fatty acid biosynthesis-associated gene expressions (down-regulation of cfa and fabA gene expressions and the up-regulation of fabD gene expression), which, in turn, modified the composition of membrane lipid (decrease in the content ratio of unsaturated fatty acids to saturated fatty acids). In addition, oxidative injury induced by PEF treatment was associated with an increase in the content of malondialdehyde. The up-regulation of cytochrome bo oxidase gene expressions (cyoA, cyoB, and cyoC) indicated that membrane damage was induced by PEF treatment, which was related to the repairing mechanism of alleviating the oxidative injury caused by PEF treatment. Based on these results, we achieved better understanding of microbial injury induced by PEF, suggesting that micro-organisms tend to decrease membrane fluidity in response to PEF treatment and, thus, a greater membrane fluidity might improve the efficiency of PEF treatment to inactivate micro-organisms.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Yun O,Zeng XA,Brennan CS,Han Zdoi
10.3390/ijms17081374subject
Has Abstractpub_date
2016-08-22 00:00:00issue
8issn
1422-0067pii
ijms17081374journal_volume
17pub_type
杂志文章abstract：:Atrial fibrillation (AF) is a type of sustained arrhythmia in humans often characterized by devastating alterations to the cardiac conduction system as well as the structure of the atria. AF can lead to decreased cardiac function, heart failure, and other complications. Long non-coding RNAs (lncRNAs) have been shown t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2018-10-24 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2015-08-20 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-04-04 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010170
更新日期：2019-12-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-09-02 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19072029
更新日期：2018-07-12 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072465
更新日期：2020-04-02 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020395
更新日期：2017-02-13 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114110
更新日期：2020-06-09 00:00:00
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journal_title：International journal of molecular sciences
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doi：10.3390/ijms19051527
更新日期：2018-05-21 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20020425
更新日期：2019-01-19 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15023287
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journal_title：International journal of molecular sciences
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更新日期：2020-09-26 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2018-12-24 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2018-05-10 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18040714
更新日期：2017-03-28 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2016-10-27 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151120117
更新日期：2014-11-05 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-08-19 00:00:00
