High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.


:Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.


Int J Mol Sci


Wójcik M,Telzerow A,Quax WJ,Boersma YL




Has Abstract


2015-10-20 00:00:00












  • Regulation of Dual-Specificity Phosphatase (DUSP) Ubiquitination and Protein Stability.

    abstract::Mitogen-activated protein kinases (MAPKs) are key regulators of signal transduction and cell responses. Abnormalities in MAPKs are associated with multiple diseases. Dual-specificity phosphatases (DUSPs) dephosphorylate many key signaling molecules, including MAPKs, leading to the regulation of duration, magnitude, or...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Chen HF,Chuang HC,Tan TH

    更新日期:2019-05-30 00:00:00

  • AMPA Receptor Auxiliary Proteins of the CKAMP Family.

    abstract::α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are assembled of four core subunits and several additional interacting proteins. Cystine-knot AMPA receptor-modulating proteins (CKAMPs) constitute a family of four proteins that influence the trafficking, subcellular localization and function of AM...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: von Engelhardt J

    更新日期:2019-03-22 00:00:00

  • Enteric Microbiota⁻Gut⁻Brain Axis from the Perspective of Nuclear Receptors.

    abstract::Nuclear receptors (NRs) play a key role in regulating virtually all body functions, thus maintaining a healthy operating body with all its complex systems. Recently, gut microbiota emerged as major factor contributing to the health of the whole organism. Enteric bacteria have multiple ways to influence their host and ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Duszka K,Wahli W

    更新日期:2018-07-28 00:00:00

  • Neuroprotective effects of sulforaphane on cholinergic neurons in mice with Alzheimer's disease-like lesions.

    abstract::Alzheimer's disease (AD) is a common neurodegenerative disease in elderly individuals, and effective therapies are unavailable. This study was designed to investigate the neuroprotective effects of sulforaphane (an activator of NF-E2-related factor 2) on mice with AD-like lesions induced by combined administration of ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zhang R,Zhang J,Fang L,Li X,Zhao Y,Shi W,An L

    更新日期:2014-08-18 00:00:00

  • Enhancement of immune activation activities of Spirulina maxima grown in deep-sea water.

    abstract::In this study, the immuno-modulatory and anticancer activities of marine algae, Spirulina maxima grown in deep-sea water (DSW), were investigated. It was found that the extract of S. maxima, cultured in DSW, effectively suppressed the expression of Bcl2 in A549 cells as well as inhibiting various human cancer cells wi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Choi WY,Kang DH,Lee HY

    更新日期:2013-06-06 00:00:00

  • Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee Melipona fasciculata.

    abstract::The stingless bee, Melipona fasciculata Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by M. fasciculata. In this context, the present study investigated the chemical comp...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lopes AJO,Vasconcelos CC,Pereira FAN,Silva RHM,Queiroz PFDS,Fernandes CV,Garcia JBS,Ramos RM,Rocha CQD,Lima STJRM,Cartágenes MDSS,Ribeiro MNS

    更新日期:2019-09-12 00:00:00

  • The Impact of Single- and Double-Strand DNA Breaks in Human Spermatozoa on Assisted Reproduction.

    abstract::Several cellular insults can result in sperm DNA fragmentation either on one or both DNA strands. Oxidative damage, premature interruption of the apoptotic process and defects in DNA compaction during spermatogenesis are the main mechanisms that cause DNA breaks in sperm. The two-tailed Comet assay is the only techniq...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Agarwal A,Barbăroșie C,Ambar R,Finelli R

    更新日期:2020-05-29 00:00:00

  • Proteomic Screening for Prediction and Design of Antimicrobial Peptides with AmpGram.

    abstract::Antimicrobial peptides (AMPs) are molecules widespread in all branches of the tree of life that participate in host defense and/or microbial competition. Due to their positive charge, hydrophobicity and amphipathicity, they preferentially disrupt negatively charged bacterial membranes. AMPs are considered an important...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Burdukiewicz M,Sidorczuk K,Rafacz D,Pietluch F,Chilimoniuk J,Rödiger S,Gagat P

    更新日期:2020-06-17 00:00:00

  • Repellent and Antifeedant Activities of Citral-Derived Lactones against the Peach Potato Aphid.

    abstract::Citral is well known for its antimicrobial, antifungal, and insecticidal activities. Natural sesquiterpene α-methylenelactones also exhibit a broad spectrum of biological activities. The aim of the study was to explore the effect of structural changes to citral molecules on citral behavior-modifying activity towards M...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dancewicz K,Szumny A,Wawrzeńczyk C,Gabryś B

    更新日期:2020-10-28 00:00:00

  • The between Now and Then of Lung Cancer Chemotherapy and Immunotherapy.

    abstract::Lung cancer is the most common cancer worldwide. Disappointingly, despite great effort in encouraging screening or, at least, a close surveillance of high-risk individuals, most of lung cancers are diagnosed when already surgically unresectable because of local advancement or metastasis. In these cases, the treatment ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Visconti R,Morra F,Guggino G,Celetti A

    更新日期:2017-06-27 00:00:00

  • Explaining the Microtubule Energy Balance: Contributions Due to Dipole Moments, Charges, van der Waals and Solvation Energy.

    abstract::Microtubules are the main components of mitotic spindles, and are the pillars of the cellular cytoskeleton. They perform most of their cellular functions by virtue of their unique dynamic instability processes which alternate between polymerization and depolymerization phases. This in turn is driven by a precise balan...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ayoub AT,Staelens M,Prunotto A,Deriu MA,Danani A,Klobukowski M,Tuszynski JA

    更新日期:2017-09-22 00:00:00

  • Slow Dissolution Kinetics of Model Peptide Fibrils.

    abstract::Understanding the kinetics of peptide self-assembly is important because of the involvement of peptide amyloid fibrils in several neurodegenerative diseases. In this paper, we have studied the dissolution kinetics of self-assembled model peptide fibrils after a dilution quench. Due to the low concentrations involved, ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Koder Hamid M,Rüter A,Kuczera S,Olsson U

    更新日期:2020-10-16 00:00:00

  • Physiological and proteomic responses of diploid and tetraploid black locust (Robinia pseudoacacia L.) subjected to salt stress.

    abstract::Tetraploid black locust (Robinia pseudoacacia L.) is adaptable to salt stress. Here, we compared morphological, physiological, ultrastructural, and proteomic traits of leaves in tetraploid black locust and its diploid relatives under salt stress. The results showed that diploid (2×) plants suffered from greater negati...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang Z,Wang M,Liu L,Meng F

    更新日期:2013-10-14 00:00:00

  • Hyaluronan-Loaded Liposomal Dexamethasone-Diclofenac Nanoparticles for Local Osteoarthritis Treatment.

    abstract::Osteoarthritis (OA) remains one of the common degenerative joint diseases and a major cause of pain and disability in older adult individuals. Oral administration of non-steroidal anti-inflammatory drugs (NSAIDs) (such as diclofenac, DIC) or intra-articular injected gluco-corticosteroids (such as dexamethasone, DEX) w...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Chang MC,Chiang PF,Kuo YJ,Peng CL,Chen KY,Chiang YC

    更新日期:2021-01-11 00:00:00

  • Administration of Zinc plus Cyclo-(His-Pro) Increases Hippocampal Neurogenesis in Rats during the Early Phase of Streptozotocin-Induced Diabetes.

    abstract::The effects of zinc supplementation on hippocampal neurogenesis in diabetes mellitus have not been studied. Herein, we investigated the effects of zinc plus cyclo-(His-Pro) (ZC) on neurogenesis occurring in the subgranular zone of dentate gyrus after streptozotocin (STZ)-induced diabetes. ZC (27 mg/kg) was administere...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Choi BY,Kim IY,Kim JH,Lee BE,Lee SH,Kho AR,Sohn M,Suh SW

    更新日期:2017-01-01 00:00:00

  • Structural Studies of Predicted Ligand Binding Sites and Molecular Docking Analysis of Slc2a4 as a Therapeutic Target for the Treatment of Cancer.

    abstract::Presently, many studies have focused on exploring in silico approaches in the identification and development of alternative therapy for the treatment and management of cancer. Solute carrier family-2-member-4-gene (Slc2a4) which encodes glucose transporter 4 protein (GLUT4), has been identified as a promising therapeu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Aruleba RT,Adekiya TA,Oyinloye BE,Kappo AP

    更新日期:2018-01-28 00:00:00

  • Exosomal Expression of CXCR4 Targets Cardioprotective Vesicles to Myocardial Infarction and Improves Outcome after Systemic Administration.

    abstract::Cell therapy has been evaluated to enhance heart function after injury. Delivered cells mostly act via paracrine mechanisms, including secreted growth factors, cytokines, and vesicles, such as exosomes (Exo). Intramyocardial injection of cardiac-resident progenitor cells (CPC)-derived Exo reduced scarring and improved...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ciullo A,Biemmi V,Milano G,Bolis S,Cervio E,Fertig ET,Gherghiceanu M,Moccetti T,Camici GG,Vassalli G,Barile L

    更新日期:2019-01-22 00:00:00

  • The Role of IgG4 in the Fine Tuning of Tolerance in IgE-Mediated Allergy and Cancer.

    abstract::Among the four immunoglobulin G (IgG) subclasses, IgG4 is the least represented in serum of a healthy human and it is considered an "odd" antibody. The IgG4 antibody has unique structural features that affect its biological function. These include the ability to undergo antigen-binding fragment (Fab)-arm exchange, to ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Bianchini R,Karagiannis SN,Jordakieva G,Jensen-Jarolim E

    更新日期:2020-07-16 00:00:00

  • Mitogen-Activated Protein Kinase Phosphatases (MKPs) in Fungal Signaling: Conservation, Function, and Regulation.

    abstract::Mitogen-activated protein kinases (MAPKs) are key mediators of signaling in fungi, participating in the response to diverse stresses and in developmental processes. Since the precise regulation of MAPKs is fundamental for cell physiology, fungi bear dual specificity phosphatases (DUSPs) that act as MAP kinase phosphat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: González-Rubio G,Fernández-Acero T,Martín H,Molina M

    更新日期:2019-04-05 00:00:00

  • Her2-Targeted Therapy Induces Autophagy in Esophageal Adenocarcinoma Cells.

    abstract::Esophageal adenocarcinoma (EAC) is a highly lethal cancer type with an overall poor survival rate. Twenty to thirty percent of EAC overexpress the human epidermal growth factor receptor 2 (Her2), a transmembrane receptor tyrosine kinase promoting cell growth and proliferation. Patients with Her2 overexpressing breast ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Janser FA,Adams O,Bütler V,Schläfli AM,Dislich B,Seiler CA,Kröll D,Langer R,Tschan MP

    更新日期:2018-10-08 00:00:00

  • Berteroin present in cruciferous vegetables exerts potent anti-inflammatory properties in murine macrophages and mouse skin.

    abstract::Berteroin (5-methylthiopentyl isothiocyanate) is a sulforaphane analog present in cruciferous vegetables, including Chinese cabbage, rucola salad leaves, and mustard oil. We examined whether berteroin exerts anti-inflammatory activities using lipopolysaccharide (LPS)-stimulated Raw 264.7 macrophages and 12-O-tetradeca...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Jung YJ,Jung JI,Cho HJ,Choi MS,Sung MK,Yu R,Kang YH,Park JH

    更新日期:2014-11-11 00:00:00

  • Consecutive Isoproterenol and Adenosine Treatment Confers Marked Protection against Reperfusion Injury in Adult but Not in Immature Heart: A Role for Glycogen.

    abstract::Consecutive treatment of adult rat heart with isoproterenol and adenosine (Iso/Aden), known to consecutively activate PKA/PKC signaling, is cardioprotective against ischemia and reperfusion (I/R). Whether this is cardioprotective in an immature heart is unknown. Langendorff-perfused hearts from adult and immature (60 ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lewis M,Szobi A,Balaska D,Khaliulin I,Adameova A,Griffiths E,Orchard CH,Suleiman MS

    更新日期:2018-02-07 00:00:00

  • Bacterial growth kinetics under a novel flexible methacrylate dressing serving as a drug delivery vehicle for antiseptics.

    abstract::A flexible methacrylate powder dressing (Altrazeal®) transforms into a wound contour conforming matrix once in contact with wound exudate. We hypothesised that it may also serve as a drug delivery vehicle for antiseptics. The antimicrobial efficacy and influence on bacterial growth kinetics in combination with three a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Forstner C,Leitgeb J,Schuster R,Dosch V,Kramer A,Cutting KF,Leaper DJ,Assadian O

    更新日期:2013-05-21 00:00:00

  • Hydrothermal Synthesis Au-Bi2Te3 Nanocomposite Thermoelectric Film with a Hierarchical Sub-Micron Antireflection Quasi-Periodic Structure.

    abstract::In this work, Au-Bi(2)Te(3) nanocomposite thermoelectric film with a hierarchical sub-micron antireflection quasi-periodic structure was synthesized via a low-temperature chemical route using Troides helena (Linnaeus) forewing (T_FW) as the biomimetic template. This method combines chemosynthesis with biomimetic techn...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Tian J,Zhang W,Zhang Y,Xue R,Wang Y,Zhang Z,Zhang D

    更新日期:2015-06-03 00:00:00

  • Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2.

    abstract::IKAROS, encoded by the IKZF1 gene, is a DNA-binding protein that functions as a tumor suppressor in T cell acute lymphoblastic leukemia (T-ALL). Recent studies have identified IKAROS's novel function in the epigenetic regulation of gene expression in T-ALL and uncovered many genes that are likely to be directly regula...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dovat E,Song C,Hu T,Rahman MA,Dhanyamraju PK,Klink M,Bogush D,Soliman M,Kane S,McGrath M,Ding Y,Desai D,Sharma A,Gowda C

    更新日期:2021-01-15 00:00:00

  • Global Transcriptome Analysis of Brown Adipose Tissue of Diet-Induced Obese Mice.

    abstract::Consumption of a high-fat diet (HFD) promotes the development of obesity, a disease resulting from an imbalance between energy intake and energy expenditure. Brown adipose tissue (BAT) has thermogenic capacity that burns calories to produce heat, and it is a potential target for the treatment and prevention of obesity...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Cao J,Zhu Q,Liu L,Glazier BJ,Hinkel BC,Liang C,Shi H

    更新日期:2018-04-06 00:00:00

  • Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels.

    abstract::Melatonin has long been recognized as a positive signaling molecule and potent antioxidant in plants, which alleviates damage caused by adverse conditions such as salt, cold, and heat stress. In this study, we found a paradoxical role for melatonin in abiotic stress responses. Suppression of the serotonin N-acetyltran...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Hwang OJ,Back K

    更新日期:2019-10-18 00:00:00

  • Combinatorial Drug Treatments Reveal Promising Anticytomegaloviral Profiles for Clinically Relevant Pharmaceutical Kinase Inhibitors (PKIs).

    abstract::Human cytomegalovirus (HCMV) is a human pathogenic herpesvirus associated with a variety of clinical symptoms. Current antiviral therapy is not always effective, so that improved drug classes and drug-targeting strategies are needed. Particularly host-directed antivirals, including pharmaceutical kinase inhibitors (PK...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wild M,Kicuntod J,Seyler L,Wangen C,Bertzbach LD,Conradie AM,Kaufer BB,Wagner S,Michel D,Eickhoff J,Tsogoeva SB,Bäuerle T,Hahn F,Marschall M

    更新日期:2021-01-08 00:00:00

  • Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels.

    abstract::Little is known about the interactions of single nucleotide polymorphisms (SNPs) and overweight/obesity on blood pressure levels. The present study was undertaken to detect 10 lipid-related gene SNPs and their interactions with overweight/obesity on blood pressure levels. Genotyping of ATP-binding cassette transporter...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,随机对照试验


    authors: Yin RX,Wu DF,Aung LH,Yan TT,Cao XL,Long XJ,Miao L,Liu WY,Zhang L,Li M

    更新日期:2012-01-01 00:00:00

  • Activation of Mechanistic Target of Rapamycin (mTOR) in Human Endothelial Cells Infected with Pathogenic Spotted Fever Group Rickettsiae.

    abstract::Attributed to the tropism for host microvascular endothelium lining the blood vessels, vascular inflammation and dysfunction represent salient features of rickettsial pathogenesis, yet the details of fundamentally important pathogen interactions with host endothelial cells (ECs) as the primary targets of infection rem...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sahni A,Narra HP,Sahni SK

    更新日期:2020-09-29 00:00:00