Abstract:
:Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wójcik M,Telzerow A,Quax WJ,Boersma YLdoi
10.3390/ijms161024918subject
Has Abstractpub_date
2015-10-20 00:00:00pages
24918-45issue
10issn
1422-0067pii
ijms161024918journal_volume
16pub_type
杂志文章,评审abstract::High levels of docosahexaenoic acid (DHA) in the phospholipids of mammalian brain have generated increasing interest in the search for its role in regulating brain functions. Recent studies have provided evidence for enhanced protective effects when DHA is administered in combination with phytochemicals, such as querc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20040932
更新日期:2019-02-21 00:00:00
abstract::Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose catalytic activities are regulated by interactions with cyclins and CDK inhibitors (CKIs). CDKs are key regulatory enzymes involved in cell proliferation through regulating cell-cycle checkpoints and transcriptional events in response to extracellular ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21061960
更新日期:2020-03-13 00:00:00
abstract::Exceptionally long-lived individuals (ELLI) who are the focus of many healthy longevity studies around the globe are now being studied in Israel. The Israeli Multi-Ethnic Centenarian Study (IMECS) cohort is utilized here for assessment of various DNA methylation clocks. Thorough phenotypic characterization and whole b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
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更新日期:2020-01-17 00:00:00
abstract::Abstract: Cancer heterogeneity and progression are subject to complex interactions between neoplastic cells and their microenvironment, including the immune system. Although glioblastomas (GBMs) are classified as 'cold tumours' with very little lymphocyte infiltration, they can contain up to 30-40% of tumour-associate...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030689
更新日期:2020-01-21 00:00:00
abstract::Helicobacter pylori is the most abundant bacterium in the gastric epithelium, and its presence has been associated with the risk of developing gastric cancer. As of 15 years ago, no other bacteria were associated with gastric epithelial colonization; but thanks to new methodologies, many other non-H. pylori bacteria h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2018-05-03 00:00:00
abstract::NRF2 (Nuclear factor Erythroid 2-related Factor 2) signaling is impaired in Friedreich's Ataxia (FRDA), an autosomal recessive disease characterized by progressive nervous system damage and degeneration of nerve fibers in the spinal cord and peripheral nerves. The loss of frataxin in patients results in iron sulfur cl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205211
更新日期:2019-10-21 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2021-01-09 00:00:00
abstract::Molecular characterization of unsequenced plant species with complex genomes is now possible by genotyping-by-sequencing (GBS) using recent next generation sequencing technologies. This study represents the first use of GBS application to sample genome-wide variants of crested wheatgrass [Agropyron cristatum (L.) Gaer...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092587
更新日期:2018-08-31 00:00:00
abstract::Biologically active chemical compounds may provide remedies for several diseases. Meanwhile, Machine Learning techniques applied to Drug Discovery, which are cheaper and faster than wet-lab experiments, have the capability to more effectively identify molecules with the expected pharmacological activity. Therefore, it...
journal_title:International journal of molecular sciences
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms20246116
更新日期:2019-12-04 00:00:00
abstract::Monoterpenoids are a diverse class of natural products and contribute to the important varietal aroma of certain Vitis vinifera grape cultivars. Among the typical monoterpenoids, linalool exists in almost all grape varieties. A gene coding for a nerolidol/linalool (NES/LINS) synthase was evaluated in the role of linal...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151221992
更新日期:2014-12-01 00:00:00
abstract::The Wnt/β-catenin pathway plays an essential role in the tumorigenesis of colorectal cancer. T-cell factor-4 (TCF4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors, and dysregulation of β-catenin is decisive for the initiation and progression of colorectal cancer. However, the rol...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160510411
更新日期:2015-05-07 00:00:00
abstract::The deletion of Mecp2, the gene encoding methyl-CpG-binding protein 2, causes severe breathing defects and developmental anomalies in mammals. In Mecp2-null mice, impaired GABAergic neurotransmission is demonstrated at the early stage of life. GABAergic dysfunction in neurons in the rostral ventrolateral medulla (RVLM...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205177
更新日期:2019-10-18 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19061569
更新日期:2018-05-24 00:00:00
abstract::Lung squamous cell carcinoma (LUSC) has a poor prognosis, in part due to poor therapeutic response and limited therapeutic alternatives. Lichens are symbiotic organisms, producing a variety of substances with multiple biological activities. (+)-Usnic acid, an important biologically active metabolite of lichens, has be...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030876
更新日期:2020-01-29 00:00:00
abstract::Efficient charge transport has been observed in iodine-doped, iodide-based room-temperature ionic liquids, yielding high ionic conductivity. To elucidate preferred mechanistic pathways for the iodide ( I - )-to-triiodide ( I 3 - ) exchange reactions, we have performed 10 ns reactive molecular-dynamics calculations in ...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms20051123
更新日期:2019-03-05 00:00:00
abstract::Leaf senescence is an important agronomic trait that affects both crop yield and quality. In this study, we characterized a premature leaf senescence mutant of wheat (Triticum aestivum L.) obtained by ethylmethane sulfonate (EMS) mutagenesis, named m68. Genetic analysis showed that the leaf senescence phenotype of m68...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030782
更新日期:2018-03-10 00:00:00
abstract::The development of neural circuits is a complex process that relies on the proper navigation of axons through their environment to their appropriate targets. While axon-environment and axon-target interactions have long been known as essential for circuit formation, communication between axons themselves has only more...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21145170
更新日期:2020-07-21 00:00:00
abstract::Osteosarcoma (OS) is the most common type of primary bone tumor. Currently, there are limited treatment options for metastatic OS. Alpha-ketoglutarate (AKG), i.e., a multifunctional intermediate of the Krebs cycle, is one of the central metabolic regulators of tumor fate and plays an important role in cancerogenesis a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249406
更新日期:2020-12-10 00:00:00
abstract::The impact of polyunsaturated fatty acid (PUFA) supplementation on phospholipase D (PLD) trafficking and activity in mast cells was investigated. The enrichment of mast cells with different PUFA including α-linolenic acid (LNA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), linoleic acid (LA) or arachidonic...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms14059005
更新日期:2013-04-25 00:00:00
abstract::This review summarizes available data on argan fruit botany, geographical distribution, traditional uses, environmental interest, socioeconomic role, phytochemistry, as well as health beneficial effects and examination of future prospects. In particular, ethnomedical uses of argan fruits are carried out throughout Mor...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms18112277
更新日期:2017-10-30 00:00:00
abstract::The recognition of cell surface glycans by lectins may be critical for the innate and adaptive immune responses. ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing TLR2 N-glycans and induces Th1 immunity. We recently demonstrated that ArtinM stimulated C...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms18071400
更新日期:2017-06-30 00:00:00
abstract::Chronic kidney disease (CKD) is associated with atherogenic dyslipidemia. Our aim was firstly to investigate patterns of fatty acids (FA) composition through various stages of CKD, and secondly, to evaluate the effect of CKD-specific FA disturbances on the expression of genes related to lipid metabolism at a cellular ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102470
更新日期:2019-05-18 00:00:00
abstract::As the worldwide application of nanomaterials in commercial products increases every year, various nanoparticles from industry might present possible risks to aquatic systems and human health. Presently, there are many unknowns about the toxic effects of nanomaterials, especially because the unique physicochemical pro...
journal_title:International journal of molecular sciences
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更新日期:2020-04-20 00:00:00
abstract::Oxymatrine (OMT) is a strong immunosuppressive agent that has been used in the clinic for many years. In the present study, by using plaque inhibition, luciferase reporter plasmids, qRT-PCR, western blotting, and ELISA assays, we have investigated the effect and mechanism of OMT on influenza A virus (IAV) replication ...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms19040965
更新日期:2018-03-23 00:00:00
abstract::Congenital dyserythropoietic anemia type II (CDA II) is a hypo-productive anemia defined by ineffective erythropoiesis through maturation arrest of erythroid precursors. CDA II is an autosomal recessive disorder due to loss-of-function mutations in SEC23B. Currently, management of patients with CDA II is based on tran...
journal_title:International journal of molecular sciences
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更新日期:2020-08-04 00:00:00
abstract::The emergence of the novel coronavirus in Wuhan, China, which causes severe respiratory tract infections in humans (COVID-19), has become a global health concern. Most coronaviruses infect animals but can evolve into strains that cross the species barrier and infect humans. At the present, there is no single specific ...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms21072272
更新日期:2020-03-25 00:00:00
abstract::Cardiovascular diseases (CVDs) are the leading causes of death worldwide. CVD pathophysiology is often characterized by increased stiffening of the heart muscle due to fibrosis, thus resulting in diminished cardiac function. Fibrosis can be caused by increased oxidative stress and inflammation, which is strongly linke...
journal_title:International journal of molecular sciences
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abstract::Spinal sarcopenia is a complex and multifactorial disorder associated with a loss of strength, increased frailty, and increased risks of fractures and falls. In addition, spinal sarcopenia has been associated with lumbar spine disorders and osteoporosis, which renders making decisions on treatment modalities difficult...
journal_title:International journal of molecular sciences
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更新日期:2020-04-24 00:00:00
abstract::Trichoderma reesei is a biotechnologically important filamentous fungus with the remarkable ability to secrete large amounts of enzymes, whose production is strongly affected by both the carbon and nitrogen sources. While the carbon metabolism regulators are extensively studied, the regulation of enzyme production by ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20174100
更新日期:2019-08-22 00:00:00