High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.

Abstract:

:Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.

journal_name

Int J Mol Sci

authors

Wójcik M,Telzerow A,Quax WJ,Boersma YL

doi

10.3390/ijms161024918

subject

Has Abstract

pub_date

2015-10-20 00:00:00

pages

24918-45

issue

10

issn

1422-0067

pii

ijms161024918

journal_volume

16

pub_type

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