High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.


:Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.


Int J Mol Sci


Wójcik M,Telzerow A,Quax WJ,Boersma YL




Has Abstract


2015-10-20 00:00:00












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    abstract::Grape hyacinth (Muscari spp.) is a popular ornamental plant with bulbous flowers noted for their rich blue color. Muscari species have been thought to accumulate delphinidin and cyanidin rather than pelargonidin-type anthocyanins because their dihydroflavonol 4-reductase (DFR) does not efficiently reduce dihydrokaempf...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Liu H,Lou Q,Ma J,Su B,Gao Z,Liu Y

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  • PLGA biodegradable nanoparticles containing perphenazine or chlorpromazine hydrochloride: effect of formulation and release.

    abstract::In our study, poly(dl-lactide-co-glycolide) (PLGA) nanoparticles loaded with perphenazine (PPH) and chlorpromazine hydrochloride (CPZ-HCl) were formulated by emulsion solvent evaporation technique. The effect of various processing variables, including PLGA concentration, theoretical drug loading, poly(vinyl alcohol) (...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Halayqa M,Domańska U

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  • The Opposite Effect of c-Jun Transcription Factor on Apolipoprotein E Gene Regulation in Hepatocytes and Macrophages.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Trusca VG,Fuior EV,Kardassis D,Simionescu M,Gafencu AV

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    abstract::Understanding how cell organelles and compartments communicate with each other has always been an important field of knowledge widely explored by many researchers. However, despite years of investigations, one point-and perhaps the only point that many agree on-is that our knowledge about cellular-signaling pathways s...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Mielecki J,Gawroński P,Karpiński S

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  • Comparative Proteomic Analysis of Wild-Type Physcomitrella Patens and an OPDA-Deficient Physcomitrella Patens Mutant with Disrupted PpAOS1 and PpAOS2 Genes after Wounding.

    abstract::Wounding is a serious environmental stress in plants. Oxylipins such as jasmonic acid play an important role in defense against wounding. Mechanisms to adapt to wounding have been investigated in vascular plants; however, those mechanisms in nonvascular plants remain elusive. To examine the response to wounding in Phy...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Luo W,Komatsu S,Abe T,Matsuura H,Takahashi K

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  • Nobiletin Inhibits Angiogenesis by Regulating Src/FAK/STAT3-Mediated Signaling through PXN in ER⁺ Breast Cancer Cells.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sp N,Kang DY,Joung YH,Park JH,Kim WS,Lee HK,Song KD,Park YM,Yang YM

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    abstract::Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang DC,Jiang CH,Zhang LN,Chen L,Zhang XY,Guo JH

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  • SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang YY,Chiang HS,Cheng CY,Wu YN,Lin YC,Liu HC,Tsai WK,Chen YL,Lin YH

    更新日期:2017-12-29 00:00:00

  • Age- and Brain Region-Specific Changes of Glucose Metabolic Disorder, Learning, and Memory Dysfunction in Early Alzheimer's Disease Assessed in APP/PS1 Transgenic Mice Using 18F-FDG-PET.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li XY,Men WW,Zhu H,Lei JF,Zuo FX,Wang ZJ,Zhu ZH,Bao XJ,Wang RZ

    更新日期:2016-10-18 00:00:00

  • The EGFR-ERK/JNK-CCL20 Pathway in Scratched Keratinocytes May Underpin Koebnerization in Psoriasis Patients.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Furue K,Ito T,Tanaka Y,Hashimoto-Hachiya A,Takemura M,Murata M,Kido-Nakahara M,Tsuji G,Nakahara T,Furue M

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  • Generation of bladder urothelium from human pluripotent stem cells under chemically defined serum- and feeder-free system.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kang M,Kim HH,Han YM

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  • Human DNA Virus Exploitation of the MAPK-ERK Cascade.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: DuShane JK,Maginnis MS

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    pub_type: 杂志文章


    authors: Xu P,Zhang M,Qian P,Li J,Wang X,Wu Y

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    pub_type: 杂志文章


    authors: Kwak DH,Moussavou G,Lee JH,Heo SY,Ko K,Hwang KA,Jekal SJ,Choo YK

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


    authors: Keller S,Schmidt MHH

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Rinsma NF,Farré R,Troost FJ,Elizalde M,Keszthelyi D,Helyes Z,Masclee AA,Conchillo JM

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Niesyto K,Neugebauer D

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Solich J,Kuśmider M,Faron-Górecka A,Pabian P,Dziedzicka-Wasylewska M

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ko MH,Hsieh YL,Hsieh ST,Tseng TJ

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  • Highly Sensitive and Specific Molecular Test for Mutations in the Diagnosis of Thyroid Nodules: A Prospective Study of BRAF-Prevalent Population.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Cho YY,Park SY,Shin JH,Oh YL,Choe JH,Kim JH,Kim JS,Yim HS,Kim YL,Ki CS,Kim TH,Chung JH,Kim SW

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Guo F,Shan Z,Yu J,Xu G,Zhang Z

    更新日期:2020-08-09 00:00:00

  • Metabolomics in Sleep, Insomnia and Sleep Apnea.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Humer E,Pieh C,Brandmayr G

    更新日期:2020-09-30 00:00:00

  • Pathophysiology of Type 2 Diabetes Mellitus.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Galicia-Garcia U,Benito-Vicente A,Jebari S,Larrea-Sebal A,Siddiqi H,Uribe KB,Ostolaza H,Martín C

    更新日期:2020-08-30 00:00:00

  • Thrombin Upregulates PAI-1 and Mesothelial-Mesenchymal Transition Through PAR-1 and Contributes to Tuberculous Pleural Fibrosis.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Hsieh CY,Sheu JR,Yang CH,Chen WL,Tsai JH,Chung CL

    更新日期:2019-10-13 00:00:00

  • Isoviolanthin Extracted from Dendrobium officinale Reverses TGF-β1-Mediated Epithelial⁻Mesenchymal Transition in Hepatocellular Carcinoma Cells via Deactivating the TGF-β/Smad and PI3K/Akt/mTOR Signaling Pathways.

    abstract::Dendrobium officinale is a precious medicinal herb and health food, and its pharmacological actions have been studied and proved. However, the mechanisms by which its active flavonoid glycosides affect epithelial⁻mesenchymal transition (EMT) in hepatocellular carcinoma (HCC) cells, such as HepG2 and Bel-7402 cells, ha...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Xing S,Yu W,Zhang X,Luo Y,Lei Z,Huang D,Lin J,Huang Y,Huang S,Nong F,Zhou C,Wei G

    更新日期:2018-05-23 00:00:00

  • Mitochondrial Carriers for Aspartate, Glutamate and Other Amino Acids: A Review.

    abstract::Members of the mitochondrial carrier (MC) protein family transport various molecules across the mitochondrial inner membrane to interlink steps of metabolic pathways and biochemical processes that take place in different compartments; i.e., are localized partly inside and outside the mitochondrial matrix. MC substrate...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Monné M,Vozza A,Lasorsa FM,Porcelli V,Palmieri F

    更新日期:2019-09-10 00:00:00

  • Ectopic Expression of Testis Germ Cell Proteins in Cancer and Its Potential Role in Genomic Instability.

    abstract::Genomic instability is a hallmark of human cancer and an enabling factor for the genetic alterations that drive cancer development. The processes involved in genomic instability resemble those of meiosis, where genetic material is interchanged between homologous chromosomes. In most types of human cancer, epigenetic c...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Nielsen AY,Gjerstorff MF

    更新日期:2016-06-06 00:00:00

  • Mesenchymal Stem Cell Secretome as an Emerging Cell-Free Alternative for Improving Wound Repair.

    abstract::The use of mesenchymal stem cells (MSC) for the treatment of cutaneous wounds is currently of enormous interest. However, the broad translation of cell therapies into clinical use is hampered by their efficacy, safety, manufacturing and cost. MSCs release a broad repertoire of trophic factors and immunomodulatory cyto...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Ahangar P,Mills SJ,Cowin AJ

    更新日期:2020-09-24 00:00:00