High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.


:Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.


Int J Mol Sci


Wójcik M,Telzerow A,Quax WJ,Boersma YL




Has Abstract


2015-10-20 00:00:00












  • Metallothioneins: Emerging Modulators in Immunity and Infection.

    abstract::Metallothioneins (MTs) are a family of metal-binding proteins virtually expressed in all organisms including prokaryotes, lower eukaryotes, invertebrates and mammals. These proteins regulate homeostasis of zinc (Zn) and copper (Cu), mitigate heavy metal poisoning, and alleviate superoxide stress. In recent years, MTs ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Subramanian Vignesh K,Deepe GS Jr

    更新日期:2017-10-23 00:00:00

  • Chromosomal Signatures Corroborate the Phylogenetic Relationships within Akodontini (Rodentia, Sigmodontinae).

    abstract::Comparative chromosome-painting analysis among highly rearranged karyotypes of Sigmodontinae rodents (Rodentia, Cricetidae) detects conserved syntenic blocks, which are proposed as chromosomal signatures and can be used as phylogenetic markers. In the Akodontini tribe, the molecular topology (Cytb and/or IRBP) shows f...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Oliveira da Silva W,Malcher SM,Pereira AL,Pieczarka JC,Ferguson-Smith MA,O'Brien PCM,Mendes-Oliveira AC,Geise L,Nagamachi CY

    更新日期:2020-03-31 00:00:00

  • Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects-In Vivo Studies on Rabbits.

    abstract::Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leachin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Krok-Borkowicz M,Reczyńska K,Rumian Ł,Menaszek E,Orzelski M,Malisz P,Silmanowicz P,Dobrzyński P,Pamuła E

    更新日期:2020-10-13 00:00:00

  • Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice.

    abstract::The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP) indu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Madokoro Y,Yoshino Y,Kato D,Sato T,Mizuno M,Kanamori T,Shimazawa M,Hida H,Hara H,Yoshida M,Borlongan CV,Ojika K,Matsukawa N

    更新日期:2019-10-28 00:00:00

  • Frontiers in Dissecting and Managing Brassica Diseases: From Reference-Based RGA Candidate Identification to Building Pan-RGAomes.

    abstract::The Brassica genus contains abundant economically important vegetable and oilseed crops, which are under threat of diseases caused by fungal, bacterial and viral pathogens. Resistance gene analogues (RGAs) are associated with quantitative and qualitative disease resistance and the identification of candidate RGAs asso...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zhang Y,Thomas W,Bayer PE,Edwards D,Batley J

    更新日期:2020-11-25 00:00:00

  • Natural products as a source for treating neglected parasitic diseases.

    abstract::Infectious diseases caused by parasites are a major threat for the entire mankind, especially in the tropics. More than 1 billion people world-wide are directly exposed to tropical parasites such as the causative agents of trypanosomiasis, leishmaniasis, schistosomiasis, lymphatic filariasis and onchocerciasis, which ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ndjonka D,Rapado LN,Silber AM,Liebau E,Wrenger C

    更新日期:2013-02-06 00:00:00

  • L-Dopa-Decarboxylase (DDC) Is a Positive Prognosticator for Breast Cancer Patients and Epinephrine Regulates Breast Cancer Cell (MCF7 and T47D) Growth In Vitro According to Their Different Expression of Gi- Protein- Coupled Receptors.

    abstract::A coherence between thyroid dysfunction and breast cancer incidence exists. Thyroid hormone metabolites bind to TAAR1 (trace amine-associated receptor 1) and through that modulate the serotonergic and dopaminergic system. Catecholamines themselves are synthesized by the L-dopa decarboxylase (DDC). The aim of our study...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Tremmel E,Kuhn C,Kaltofen T,Vilsmaier T,Mayr D,Mahner S,Ditsch N,Jeschke U,Vattai A

    更新日期:2020-12-15 00:00:00

  • CX3CR1 Deficiency Attenuates DNFB-Induced Contact Hypersensitivity Through Skewed Polarization Towards M2 Phenotype in Macrophages.

    abstract::CX3CL1 can function as both an adhesion molecule and a chemokine for CX3CR1+ cells, such as T cells, monocytes, and NK cells. Recent studies have demonstrated that CX3CL1-CX3CR1 interaction is associated with the development of various inflammatory skin diseases. In this study, we examined CX3CR1 involvement in 2,4-di...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Otobe S,Hisamoto T,Miyagaki T,Morimura S,Suga H,Sugaya M,Sato S

    更新日期:2020-10-07 00:00:00

  • RNA Chaperone Function of a Universal Stress Protein in Arabidopsis Confers Enhanced Cold Stress Tolerance in Plants.

    abstract::The physiological function of Arabidopsis thaliana universal stress protein (AtUSP) in plant has remained unclear. Thus, we report here the functional role of the Arabidopsis universal stress protein, AtUSP (At3g53990). To determine how AtUSP affects physiological responses towards cold stress, AtUSP overexpression (A...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Melencion SMB,Chi YH,Pham TT,Paeng SK,Wi SD,Lee C,Ryu SW,Koo SS,Lee SY

    更新日期:2017-11-27 00:00:00

  • Chronic Kidney Disease and Exposure to Nephrotoxic Metals.

    abstract::Chronic kidney disease (CKD) is a common progressive disease that is typically characterized by the permanent loss of functional nephrons. As injured nephrons become sclerotic and die, the remaining healthy nephrons undergo numerous structural, molecular, and functional changes in an attempt to compensate for the loss...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Orr SE,Bridges CC

    更新日期:2017-05-12 00:00:00

  • Endometrial Stromal Cells Circulate in the Bloodstream of Women with Endometriosis: A Pilot Study.

    abstract::Endometriosis is characterized by the presence of endometrial tissue outside the uterus. While endometriotic tissue is commonly localized in the pelvic cavity, it can also be found in distant sites, including the brain. The origin and pathophysiology of tissue migration is poorly understood; retrograde menstruation is...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Vallvé-Juanico J,López-Gil C,Ballesteros A,Santamaria X

    更新日期:2019-07-31 00:00:00

  • Conversion of Isocyanide to Amine in The Presence of Water and Hg(II) Ions: Kinetics and Mechanism as Detected by Fluorescence Spectroscopy and Mass Spectrometry.

    abstract::Aromatic isocyanides including isocyanonaphthalene derivatives have been proven to be very effective fluorescent sensors for the quantification of Hg(II) ions in water. Thus, the reaction of 1,5-isocyanoaminonaphthalene (1,5-ICAN), which is one of the most important members of this family, with water and HgCl2 as the ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Adamoczky A,Nagy L,Nagy M,Zsuga M,Kéki S

    更新日期:2020-08-04 00:00:00

  • The Interaction of the Senescent and Adjacent Breast Cancer Cells Promotes the Metastasis of Heterogeneous Breast Cancer Cells through Notch Signaling.

    abstract::Chemotherapy is one of the most common strategies for tumor treatment but often associated with post-therapy tumor recurrence. While chemotherapeutic drugs are known to induce tumor cell senescence, the roles and mechanisms of senescence in tumor recurrence remain unclear. In this study, we used doxorubicin to induce ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zhang N,Ji J,Zhou D,Liu X,Zhang X,Liu Y,Xiang W,Wang M,Zhang L,Wang G,Huang B,Lu J,Zhang Y

    更新日期:2021-01-15 00:00:00

  • Therapeutic Potential of Antimicrobial Peptides in Polymicrobial Biofilm-Associated Infections.

    abstract::It is widely recognized that many chronic infections of the human body have a polymicrobial etiology. These include diabetic foot ulcer infections, lung infections in cystic fibrosis patients, periodontitis, otitis, urinary tract infections and even a proportion of systemic infections. The treatment of mixed infection...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Batoni G,Maisetta G,Esin S

    更新日期:2021-01-06 00:00:00

  • Diverse Actions of Astrocytes in GABAergic Signaling.

    abstract::An imbalance of excitatory and inhibitory neurotransmission leading to over excitation plays a crucial role in generating seizures, while enhancing GABAergic mechanisms are critical in terminating seizures. In recent years, it has been reported in many studies that astrocytes are deeply involved in synaptic transmissi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Ishibashi M,Egawa K,Fukuda A

    更新日期:2019-06-18 00:00:00

  • Effect of temperature on the physico-chemical properties of a room temperature ionic liquid (1-Methyl-3-pentylimidazolium hexafluorophosphate) with polyethylene glycol oligomer.

    abstract::A systematic study of the effect of composition on the thermo-physical properties of the binary mixtures of 1-methyl-3-pentyl imidazolium hexafluorophosphate [MPI][PF(6)] with poly(ethylene glycol) (PEG) [M(w) = 400] is presented. The excess molar volume, refractive index deviation, viscosity deviation, and surface te...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wu TY,Chen BK,Hao L,Peng YC,Sun IW

    更新日期:2011-01-01 00:00:00

  • Inhibition of NADPH Oxidase Activation by Apocynin Rescues Seizure-Induced Reduction of Adult Hippocampal Neurogenesis.

    abstract::Apocynin, also known as acetovanillone, is a natural organic compound structurally related to vanillin. Apocynin is known to be an inhibitor of NADPH (Nicotinamide adenine dinucleotide phosphate) oxidase activity and is highly effective in suppressing the production of superoxide. The neuroprotective effects of apocyn...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lee SH,Choi BY,Kho AR,Jeong JH,Hong DK,Kang DH,Kang BS,Song HK,Choi HC,Suh SW

    更新日期:2018-10-09 00:00:00

  • Investigating ionic effects applied to water based organocatalysed aldol reactions.

    abstract::Saturated aqueous solutions of various common salts were examined for their effect on aqueous aldol reactions catalysted by a highly active C(2)-symmetric diprolinamide organocatalyst developed in our laboratory. With respect to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde, deionised water was alwa...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Delaney JP,Henderson LC

    更新日期:2011-01-01 00:00:00

  • Function and Clinical Implications of Long Non-Coding RNAs in Melanoma.

    abstract::Metastatic melanoma is the most deadly type of skin cancer. Despite the success of immunotherapy and targeted agents, the majority of patients experience disease recurrence upon treatment and die due to their disease. Long non-coding RNAs (lncRNAs) are a new subclass of non-protein coding RNAs involved in (epigenetic)...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Richtig G,Ehall B,Richtig E,Aigelsreiter A,Gutschner T,Pichler M

    更新日期:2017-03-28 00:00:00

  • Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis.

    abstract::The homeostatic control of lipid metabolism is essential for many fundamental physiological processes. A deep understanding of its regulatory mechanisms is pivotal to unravel prospective physiopathological factors and to identify novel molecular targets that could be employed to design promising therapies in the manag...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Tonini C,Colardo M,Colella B,Di Bartolomeo S,Berardinelli F,Caretti G,Pallottini V,Segatto M

    更新日期:2020-02-14 00:00:00

  • Flexible NAD+ Binding in Deoxyhypusine Synthase Reflects the Dynamic Hypusine Modification of Translation Factor IF5A.

    abstract::The eukaryotic and archaeal translation factor IF5A requires a post-translational hypusine modification, which is catalyzed by deoxyhypusine synthase (DHS) at a single lysine residue of IF5A with NAD+ and spermidine as cofactors, followed by hydroxylation to form hypusine. While human DHS catalyzed reactions have been...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Chen M,Gai Z,Okada C,Ye Y,Yu J,Yao M

    更新日期:2020-07-31 00:00:00

  • Central Apolipoprotein A-IV Stimulates Thermogenesis in Brown Adipose Tissue.

    abstract::Stimulation of thermogenesis in brown adipose tissue (BAT) could have far-reaching health benefits in combatting obesity and obesity-related complications. Apolipoprotein A-IV (ApoA-IV), produced by the gut and the brain in the presence of dietary lipids, is a well-known short-term satiating protein. While our previou...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Pence S,LaRussa Z,Shen Z,Liu M,Coschigano KT,Shi H,Lo CC

    更新日期:2021-01-27 00:00:00

  • Aspidosperma pyrifolium Has Anti-Inflammatory Properties: An Experimental Study in Mice with Peritonitis Induced by Tityus serrulatus Venom or Carrageenan.

    abstract::Scorpions of the genus Tityus are responsible for the majority of envenomation in Brazil, the Tityus serrulatus species being the most common and dangerous in South America. In this approach, we have investigated the ability of the aqueous extract from the leaves of Aspidosperma pyrifolium in reducing carrageenan-indu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Souza Lima MCJ,Oliveira Bitencourt MA,Furtado AA,Torres-Rêgo M,Siqueira EMDS,Oliveira RM,Oliveira Rocha HA,Ferreira Rocha KB,Silva-Júnior AAD,Zucolotto SM,Fernandes-Pedrosa MF

    更新日期:2017-11-11 00:00:00

  • Syntenin: PDZ Protein Regulating Signaling Pathways and Cellular Functions.

    abstract::Syntenin is an adaptor-like molecule that has two adjacent tandem postsynaptic density protein 95/Discs large protein/Zonula occludens 1 (PDZ) domains. The PDZ domains of syntenin recognize multiple peptide motifs with low to moderate affinity. Many reports have indicated interactions between syntenin and a plethora o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Shimada T,Yasuda S,Sugiura H,Yamagata K

    更新日期:2019-08-26 00:00:00

  • Progranulin and Its Related MicroRNAs after Status Epilepticus: Possible Mechanisms of Neuroprotection.

    abstract::The current knowledge about neuroprotective mechanisms in humans after status epilepticus is scarce. One reason is the difficulty to measure possible mediators of these neuroprotective mechanisms. The dawn of microRNA detection in the cerebrospinal fluid (CSF) and the recent advancements in measuring proteins in the C...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Körtvelyessy P,Huchtemann T,Heinze HJ,Bittner DM

    更新日期:2017-02-24 00:00:00

  • Genome-Wide Identification and Analysis of Polygalacturonase Genes in Solanum lycopersicum.

    abstract::Polygalacturonase (PG), a large hydrolase family in plants, is involved in pectin disassembly of the cell wall in plants. The present study aims to characterize PG genes and investigate their expression patterns in Solanum lycopersicum. We identified 54 PG genes in the tomato genome and compared their amino acid seque...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ke X,Wang H,Li Y,Zhu B,Zang Y,He Y,Cao J,Zhu Z,Yu Y

    更新日期:2018-08-04 00:00:00

  • Cancer Vaccine Immunotherapy with RNA-Loaded Liposomes.

    abstract::Cancer vaccines may be harnessed to incite immunity against poorly immunogenic tumors, however they have failed in therapeutic settings. Poor antigenicity coupled with systemic and intratumoral immune suppression have been significant drawbacks. RNA encoding for tumor associated or specific epitopes can serve as a mor...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Sayour EJ,Mendez-Gomez HR,Mitchell DA

    更新日期:2018-09-23 00:00:00

  • Intestinal Permeability and Cellular Antioxidant Activity of Phenolic Compounds from Mango (Mangifera indica cv. Ataulfo) Peels.

    abstract::Mango (Mangifera indica cv. Ataulfo) peel contains bound phenolics that may be released by alkaline or acid hydrolysis and may be converted into less complex molecules. Free phenolics from mango cv. Ataulfo peel were obtained using a methanolic extraction, and their cellular antioxidant activity (CAA) and permeability...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Pacheco-Ordaz R,Antunes-Ricardo M,Gutiérrez-Uribe JA,González-Aguilar GA

    更新日期:2018-02-08 00:00:00

  • Structural Basis of Native CXCL7 Monomer Binding to CXCR2 Receptor N-Domain and Glycosaminoglycan Heparin.

    abstract::CXCL7, a chemokine highly expressed in platelets, orchestrates neutrophil recruitment during thrombosis and related pathophysiological processes by interacting with CXCR2 receptor and sulfated glycosaminoglycans (GAG). CXCL7 exists as monomers and dimers, and dimerization (~50 μM) and CXCR2 binding (~10 nM) constants ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Brown AJ,Sepuru KM,Rajarathnam K

    更新日期:2017-02-26 00:00:00

  • Recent Understandings of Biology, Prophylaxis and Treatment Strategies for Hypertrophic Scars and Keloids.

    abstract::Hypertrophic scars and keloids are fibroproliferative disorders that may arise after any deep cutaneous injury caused by trauma, burns, surgery, etc. Hypertrophic scars and keloids are cosmetically problematic, and in combination with functional problems such as contractures and subjective symptoms including pruritus,...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,meta分析,评审


    authors: Lee HJ,Jang YJ

    更新日期:2018-03-02 00:00:00