Abstract：
:Most species of the genus Bifidobacterium contain the gene cluster PFNA, which is presumably involved in the species-specific communication between bacteria and their hosts. The gene cluster PFNA consists of five genes including fn3, which codes for a protein containing two fibronectin type III domains. Each fibronectin domain contains sites similar to cytokine-binding sites of human receptors. Based on this finding we assumed that this protein would bind specifically to human cytokines in vitro. We cloned a fragment of the fn3 gene (1503 bp; 501 aa) containing two fibronectin domains, from the strain B. longum subsp. longum GT15. After cloning the fragment into the expression vector pET16b and expressing it in E. coli, the protein product was purified to a homogenous state for further analysis. Using the immunoferment method, we tested the purified fragment's ability to bind the following human cytokines: IL-1β, IL-6, IL-10, TNFα. We developed a sandwich ELISA system to detect any specific interactions between the purified protein and any of the studied cytokines. We found that the purified protein fragment only binds to TNFα.
journal_name
Anaerobejournal_title
Anaerobeauthors
Dyakov IN,Mavletova DA,Chernyshova IN,Snegireva NA,Gavrilova MV,Bushkova KK,Dyachkova MS,Alekseeva MG,Danilenko VNdoi
10.1016/j.anaerobe.2020.102247subject
Has Abstractpub_date
2020-10-01 00:00:00pages
102247eissn
1075-9964issn
1095-8274pii
S1075-9964(20)30103-7journal_volume
65pub_type
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