Abstract:
:Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state. Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts. However, such grafts may not be readily available, and procedural complications may result in undesired outcomes. Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts. In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo. First, scaffolds embedded with cells were characterized for cell morphology, viability, and attachment. The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro. Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture. Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone. These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jahan K,Manickam G,Tabrizian M,Murshed Mdoi
10.1038/s41598-020-67886-7subject
Has Abstractpub_date
2020-07-14 00:00:00pages
11603issue
1issn
2045-2322pii
10.1038/s41598-020-67886-7journal_volume
10pub_type
杂志文章abstract::Autonomic modulation and cardiorespiratory variables are influenced by numerous factors, including anthropometric variables. We investigated autonomic recovery following aerobic exercise in healthy men with different waist-stature ratio (WSR) values. The study was conducted with 52 healthy men aged 18 to 30 years, div...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-018-34246-5
更新日期:2018-10-31 00:00:00
abstract::Starch is the main storage carbohydrate in higher plants. Although several enzymes and regulators for starch biosynthesis have been characterized, a complete regulatory network for starch synthesis in cereal seeds remains elusive. Here, we report the identification and characterization of the rice Brittle1 (OsBT1) gen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40124
更新日期:2017-01-05 00:00:00
abstract::Caffeine modifies vascular/cardiac contractility. Embryonic exposure to caffeine altered cardiac functions in offspring. This study determined chronic influence of prenatal caffeine on vessel functions in offspring. Pregnant Sprague-Dawley rats (5-month-old) were exposed to high dose of caffeine, their offspring (5-mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13225
更新日期:2015-08-17 00:00:00
abstract::A large amount of energy, perhaps twice the total amount of all other hydrocarbon reserves combined, is trapped within gas hydrate deposits. Despite emerging as a potential energy source for the world over the next several hundred years and one of the key factors in causing future climate change, gas hydrate is poorly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06717-8
更新日期:2017-07-25 00:00:00
abstract::De novo mutations (DNMs), including germinal and postzygotic mutations (PZMs), are a strong source of causality for Autism Spectrum Disorder (ASD). However, the biological processes involved behind them remain unexplored. Our aim was to detect DNMs (germinal and PZMs) in a Spanish ASD cohort (360 trios) and to explore...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79412-w
更新日期:2021-01-11 00:00:00
abstract::L-Ascorbic acid (vitamin C, AA) exhibits anti-cancer effects with high-dose treatment through the generation of reactive oxygen species (ROS) and selective damage to cancer cells. The anti-cancer effects of L-ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29386-7
更新日期:2018-07-27 00:00:00
abstract::Priming improves an organism's performance upon a future stress. To test whether cold priming supports protection in spring and how it is affected by cold acclimation, we compared seven Arabidopsis accessions with different cold acclimation potentials in the field and in the greenhouse for growth, photosynthetic perfo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44055
更新日期:2017-03-09 00:00:00
abstract::MicroRNA is responsible for the fine-tuning of fundamental cellular activities and human disease development. The altered availability of microRNAs, target mRNAs, and other types of endogenous RNAs competing for microRNA interactions reflects the dynamic and conditional property of microRNA-mediated gene regulation th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13470-5
更新日期:2017-10-17 00:00:00
abstract::Soil microorganisms play a crucial role in the biogeochemical cycling of nutrient elements and maintaining soil health. We aimed to investigate the response of bacteria communities to organic farming over different crops (rice, tea and vegetable) along the middle and lower reaches of the Yangtze River of China. Compar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35046
更新日期:2016-10-11 00:00:00
abstract::Zinc oxy-selenide Zn(O,Se) is a novel material, that can replace the toxic CdS buffer layer in thin film solar cells and other optoelectronic devices. In this paper a systematic study of the structural, optical and electrical properties of Zn(O,Se) layers, grown by pulsed laser deposition under 50 mTorr of nitrogen ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54008-1
更新日期:2019-11-25 00:00:00
abstract::The brain-derived neurotrophic factor BDNF plays a critical role in neuronal development and the induction of L-LTP at glutamatergic synapses in several brain regions. However, the cellular and molecular mechanisms underlying these BDNF effects have not been firmly established. Using in vitro cultures of cortical neur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14778
更新日期:2015-10-06 00:00:00
abstract::Human risk-taking attitudes can be influenced by two logically equivalent but descriptively different frames, termed the framing effect. The classic hypothetical vignette-based task (Asian disease problem) and a recently developed reward-based gambling task have been widely used to assess individual differences in the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30071
更新日期:2016-07-20 00:00:00
abstract::Dirac-like surface states on surfaces of topological insulators have a chiral spin structure with spin locked to momentum, which is interesting in physics and may also have important applications in spintronics. In this work, by measuring the tunable helicity-dependent photocurrent (HDP), we present an identification ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04889
更新日期:2014-05-08 00:00:00
abstract::Carotenoids are anti-oxidative agents. Human skin and eyes contain specific carotenoid species known to prevent various pathologies caused by oxidative stress. We quantified skin and eye carotenoid levels and investigated their potential correlation in a population including 985 Japanese patients and staff members of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45751-6
更新日期:2019-06-27 00:00:00
abstract::Patients with liver cirrhosis are susceptible to infections with high short-term mortalities. One CD97-related EGF-TM7 molecule, EMR2 (EGF-like molecule containing mucin-like hormone receptor 2), had been shown to regulate human neutrophil function, potentiate systemic inflammation. Nevertheless, EMR2 could also suppr...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep38250
更新日期:2016-12-01 00:00:00
abstract::Hexane, dichloromethane and methanol extracts of the roots of Piper sarmentosum Roxb. were screened for toxicity towards Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Plodia interpunctella (Hübner) and the hexane extract exhibited the highest mortality percentage. Bioassay-guided fractionation of the hexane e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12898-z
更新日期:2017-10-03 00:00:00
abstract::As an emerging sterilization technology, cold atmospheric plasma offers a dry, non-thermal, rapid process that is minimally damaging to a majority of substrates. However, the mechanisms by which plasma interacts with living cells are poorly understood and the plasma generation apparatuses are complex and resource-inte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35166-0
更新日期:2018-11-12 00:00:00
abstract::Proton pump inhibitors (PPIs) belong to the most common medication in geriatric medicine. They are known to reduce osteoclast activity and to delay fracture healing in young adult mice. Because differentiation and proliferation in fracture healing as well as pharmacologic actions of drugs markedly differ in the elderl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79605-3
更新日期:2020-12-23 00:00:00
abstract::Pancreatic adenocarcinoma upregulated factor (PAUF) is a ligand of toll-like receptors (TLRs) and has been reported to be involved in pancreatic tumor development. However, the significance of PAUF expression in epithelial ovarian cancer remains unclear. We aimed to investigate the possible clinical significance of PA...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30582-8
更新日期:2018-08-15 00:00:00
abstract::Hyperspectral imaging (HSI) is being explored in endoscopy as a tool to extract biochemical information that may improve contrast for early cancer detection in the gastrointestinal tract. Motion artefacts during medical endoscopy have traditionally limited HSI application, however, recent developments in the field hav...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60574-6
更新日期:2020-03-03 00:00:00
abstract::Regulatory T cells (Tregs) are a double-edged regulator of the immune system. Aberrations of Tregs correlate with pathogenesis of inflammatory, autoimmune and neoplastic disorders. Phenotypically and functionally distinct subsets of Tregs have been identified in humans and mice on the basis of their extensive portfoli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50065-8
更新日期:2019-09-17 00:00:00
abstract::The human β-defensin-1 (HBD1) is an antimicrobial peptide constitutively expressed by epithelial cells at mucosal surfaces. In addition to its microbicidal properties, the loss of HBD1 expression in several cancers suggests that it may also have an anti-tumor activity. Here, we investigated the link between HBD1 expre...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36387-z
更新日期:2018-12-21 00:00:00
abstract::Leukemia is the most commonly diagnosed childhood cancer, although its etiology is still largely unknown. Growing evidence supports a role for infection in the etiology of acute lymphocytic leukemia (ALL), and the involvement of the immune system suggests that vaccination may also play a role. However, the findings pr...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-017-16067-0
更新日期:2017-11-22 00:00:00
abstract::Genotyping-by-sequencing (GBS) or restriction-site associated DNA marker sequencing (RAD-seq) is a practical and cost-effective method for analysing large genomes from high diversity species. This method of sequencing, coupled with methylation-sensitive enzymes (often referred to as methylation-sensitive restriction e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19655-w
更新日期:2018-02-01 00:00:00
abstract::BST-2/tetherin is an interferon-inducible antiviral protein that blocks the release of various enveloped viruses, including HIV-1. Hepatitis B virus (HBV), a major cause of liver disease, belongs to the Hepadnaviridae family of enveloped DNA viruses. Whether BST-2 regulates HBV production is largely unknown. In this r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11736
更新日期:2015-06-29 00:00:00
abstract::The 2b protein of Cucumber mosaic virus has a role in nearly all steps of the viral cycle including cell-to-cell movement, symptom induction and suppression of antiviral RNA silencing. Previous studies demonstrated the presence of 2b protein in the nucleus and in cytoplasm as well. Phosphorylation site of 2b protein i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13870-7
更新日期:2017-10-18 00:00:00
abstract::Sepsis is an infection-induced systemic inflammatory syndrome and a major cause of death for critically ill patients. Here, we examined whether the absence of Sprouty-related EVH1-domain-containing protein 2 (Spred2), a negative regulator of the Ras/Raf/ERK/MAPK pathway, influences host defense against polymicrobial s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13204-7
更新日期:2017-10-09 00:00:00
abstract::Magnesium-based thermoelectric materials (Mg2X, X = Si, Ge, Sn) have received considerable attention due to their availability, low toxicity, and reasonably good thermoelectric performance. The synthesis of these materials with high purity is challenging, however, due to the reactive nature and high vapour pressure of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04348-7
更新日期:2017-06-21 00:00:00
abstract::We study the impact of the direction of magnetic flux on the electron motion in GaAs/InAs core/shell nanowires. At small tilt angles, when the magnetic field is aligned nearly parallel to the nanowire axis, we observe Aharonov-Bohm type h/e flux periodic magnetoconductance oscillations. These are attributed to transpo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24573
更新日期:2016-04-19 00:00:00
abstract::A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-32121-x
更新日期:2018-10-16 00:00:00