msgbsR: An R package for analysing methylation-sensitive restriction enzyme sequencing data.

Abstract:

:Genotyping-by-sequencing (GBS) or restriction-site associated DNA marker sequencing (RAD-seq) is a practical and cost-effective method for analysing large genomes from high diversity species. This method of sequencing, coupled with methylation-sensitive enzymes (often referred to as methylation-sensitive restriction enzyme sequencing or MRE-seq), is an effective tool to study DNA methylation in parts of the genome that are inaccessible in other sequencing techniques or are not annotated in microarray technologies. Current software tools do not fulfil all methylation-sensitive restriction sequencing assays for determining differences in DNA methylation between samples. To fill this computational need, we present msgbsR, an R package that contains tools for the analysis of methylation-sensitive restriction enzyme sequencing experiments. msgbsR can be used to identify and quantify read counts at methylated sites directly from alignment files (BAM files) and enables verification of restriction enzyme cut sites with the correct recognition sequence of the individual enzyme. In addition, msgbsR assesses DNA methylation based on read coverage, similar to RNA sequencing experiments, rather than methylation proportion and is a useful tool in analysing differential methylation on large populations. The package is fully documented and available freely online as a Bioconductor package ( https://bioconductor.org/packages/release/bioc/html/msgbsR.html ).

journal_name

Sci Rep

journal_title

Scientific reports

authors

Mayne BT,Leemaqz SY,Buckberry S,Rodriguez Lopez CM,Roberts CT,Bianco-Miotto T,Breen J

doi

10.1038/s41598-018-19655-w

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

2190

issue

1

issn

2045-2322

pii

10.1038/s41598-018-19655-w

journal_volume

8

pub_type

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