Abstract:
:Antimicrobial peptides (AMPs) are an important part of the human innate immune system for protection against bacterial infections, however the AMPs display varying degrees of activity against Staphylococcus aureus. Previously, we showed that inactivation of the ATP synthase sensitizes S. aureus towards the AMP antibiotic class of polymyxins. Here we wondered if the ATP synthase similarly is needed for tolerance towards various human AMPs, including human β-defensins (hBD1-4), LL-37 and histatin 5. Importantly, we find that the ATP synthase mutant (atpA) is more susceptible to killing by hBD4, hBD2, LL-37 and histatin 5 than wild type cells, while no changes in susceptibility was detected for hBD3 and hBD1. Administration of the ATP synthase inhibitor, resveratrol, sensitizes S. aureus towards hBD4-mediated killing. Neutrophils rely on AMPs and reactive oxygen molecules to eliminate bacteria and the atpA mutant is more susceptible to killing by neutrophils than the WT, even when the oxidative burst is inhibited.These results show that the staphylococcal ATP synthase enhance tolerance of S. aureus towards some human AMPs and this indicates that inhibition of the ATP synthase may be explored as a new therapeutic strategy that sensitizes S. aureus to naturally occurring AMPs of the innate immune system.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Liu L,Beck C,Nøhr-Meldgaard K,Peschel A,Kretschmer D,Ingmer H,Vestergaard Mdoi
10.1038/s41598-020-68146-4subject
Has Abstractpub_date
2020-07-09 00:00:00pages
11391issue
1issn
2045-2322pii
10.1038/s41598-020-68146-4journal_volume
10pub_type
杂志文章abstract::TET aminopeptidases are dodecameric particles shared in the three life domains involved in various biological processes, from carbon source provider in archaea to eye-pressure regulation in humans. Each subunit contains a dinuclear metal site (M1 and M2) responsible for the enzyme catalytic activity. However, the role...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20876
更新日期:2016-02-08 00:00:00
abstract::We aimed to investigate the changes of olfaction of major depressive disorder (MDD) before and after medical treatment, and to preliminarily scrutinize the association between the olfactory function and the severity of depressive symptoms, response inhibition, and emotional responding. Forty-eight medicine-naïve MDD p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63416-7
更新日期:2020-04-14 00:00:00
abstract::Mucosal melanomas are primary malignant neoplasias originated from melanocytes within mucous membranes in any part of mucosal surface lining, more commonly, in the nasal cavity and accessory sinuses, oral cavity, lips, pharynx, vulvar, vaginal, cervix and anorectal mucosa. Epidemiology data regarding mucosal melanomas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57253-6
更新日期:2020-01-16 00:00:00
abstract::Miscanthus is a promising lignocellulosic bioenergy crop for bioethanol production. To identify candidate genes and regulation networks involved in secondary cell wall (SCW) development in Miscanthus, we performed de novo transcriptome analysis of a developing internode. According to the histological and in-situ histo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08690-8
更新日期:2017-08-22 00:00:00
abstract::Nine new bicyclic cembranoids, sarcophytrols M-U(1-9), were isolated from the South China Sea soft coral Sarcophyton trocheliophorum as minor components, along with one known related cembranoid 10. Their structures were elucidated by detailed spectroscopic analysis and chemical conversion. The chemical structures of t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46584
更新日期:2017-04-21 00:00:00
abstract::Phaeocystis globosa, one of the most typical red tide-forming species, is usually mixed in the food composition of rotifers. To explore how rotifers respond by adjusting life history strategy when feeding on different quality foods, we exposed the rotifer Brachionus plicatilis to cultures with 100% Chlorella, a mixtur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15863-y
更新日期:2017-11-13 00:00:00
abstract::We introduce "sense, track and separate" approach for the removal of Hg(2+) ion from aqueous media using highly ordered and magnetic mesoporous ferrosilicate nanocages functionalised with rhodamine fluorophore derivative. These functionalised materials offer both fluorescent and magnetic properties in a single system ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21820
更新日期:2016-02-25 00:00:00
abstract::Dissecting the complex molecular interplay between the host plant and invading virus improves our understanding of the mechanisms underlying viral pathogenesis. In this study, immunoprecipitation together with the mass spectrometry analysis revealed that the heat shock protein 70 (Hsp70) family homolog, Hsc70-2, was c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22778-9
更新日期:2018-03-14 00:00:00
abstract::Duchenne muscular dystrophy, one of the most common lethal genetic disorders, is caused by mutations in the DMD gene and a lack of dystrophin protein. In most DMD patients and animal models, sporadic dystrophin-positive muscle fibres, called revertant fibres (RFs), are observed in otherwise dystrophin-negative backgro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38371
更新日期:2016-12-07 00:00:00
abstract::Cytometric studies utilizing flow cytometry or multi-well culture plate fluorometry are often limited by a deficit in temporal resolution and a lack of single cell consideration. Unfortunately, many cellular processes, including signaling, motility, and molecular transport, occur transiently over relatively short peri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05117
更新日期:2014-05-30 00:00:00
abstract::Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) phenotype in cattle, sheep, dog and human. However, there has not been reported significant muscle phenotypes in pigs in association with MSTN mutations. Pigs...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14435
更新日期:2015-09-24 00:00:00
abstract::A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24599
更新日期:2016-04-19 00:00:00
abstract::Influenza virus evolves constantly in an unpredictable fashion, making it necessary to vaccinate people annually for effective prevention and control of influenza. In general, however, during the first wave of an influenza outbreak caused by a newly emerging virus strain, influenza morbidity and mortality have been ob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28729
更新日期:2016-06-29 00:00:00
abstract::The association of fasting glucose with the risk of death according to sex and age remains unclear, and insufficient information is available on sex- and age-specific glucose concentrations within ethnic groups. This study analyzed a sample of 12,455,361 Korean adults who participated in health examinations during 200...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08498-6
更新日期:2017-08-15 00:00:00
abstract::A micro-boat self-propelled by a camphor engine, carrying seed crystals of FeCl3, promoted the evolution of chemical gardens when placed on the surface of aqueous solutions of potassium hexacyanoferrate. Inverse chemical gardens (growing from the top downward) were observed. The growth of the "inverse" chemical garden...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04337-w
更新日期:2017-06-21 00:00:00
abstract::We report on a highly efficient magneto-actuated cancer cell apoptosis method using a biaxial pulsed magnetic field configuration, which maximizes the induced magnetic torque. The light transmissivity dynamics show that the biaxial magnetic field configuration can actuate the magnetic nanoparticles with higher respons...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11279-w
更新日期:2017-09-07 00:00:00
abstract::Regulatory T-cells (Tregs) play a pivotal role in maintaining peripheral tolerance. Increasing Treg numbers/functions has been shown to ameliorate autoimmune diseases. However, common Treg expansion approaches use T-Cell Receptor (TCR)-mediated stimulation which also causes proliferation of effector T-cells (Teff). To...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39751
更新日期:2017-01-03 00:00:00
abstract::Male infertility due to spermatogenesis defects affects millions of men worldwide. However, the genetic etiology of the vast majority remains unclear. Here we describe three men with primary infertility due to multiple morphological abnormalities of the sperm flagella (MMAF) from two unrelated Han Chinese families. We...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-019-52436-7
更新日期:2019-11-01 00:00:00
abstract::Coastal wetlands are important carbon sinks globally, but their ability to store carbon hinges on their nitrogen (N) supply and N uptake dynamics of dominant plant species. In terrestrial ecosystems, uptake of nitrate (NO3-) and ammonium (NH4+) through roots can strongly influence N acquisition rates and their respons...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23349-8
更新日期:2018-03-29 00:00:00
abstract::The overlap between pain and reward processing pathways leds researchers to hypothesize that there are interactions between them in the human brain. Two hypotheses have been proposed. The "competition hypothesis" posits that reward can reduce pain-related neural activity and vice versa. The "salience hypothesis" sugge...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26426
更新日期:2016-05-19 00:00:00
abstract::Finding overlapping communities of complex networks remains a challenge in network science. To address this challenge, one of the widely used approaches is finding the communities of links by optimizing the objective function, partition density. In this study, we show that partition density suffers from inverse resolu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12432-1
更新日期:2017-09-29 00:00:00
abstract::The crystallization characteristics in supercooled liquid Zn during isothermal relaxation were investigated using molecular dynamics simulations by adopting the cluster-type index method (CTIM) and the tracing method. Results showed that the crystallization process undergo three different stages. The size of the criti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31653
更新日期:2016-08-16 00:00:00
abstract::Self-healing is a capacity observed in most biological systems in which the healing processes are autonomously triggered after the damage. Inspired by this natural behavior, researchers believed that a synthetic material possessing similar self-recovery capability could also be developed. Albeit various intrinsic self...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30804
更新日期:2016-08-22 00:00:00
abstract::Nontuberculous mycobacteria (NTM) are environmental bacteria increasingly associated to public health problems. In water systems, free-living amoebae (FLA) feed on bacteria by phagocytosis, but several bacteria, including many NTM, are resistant to this predation. Thus, FLA can be seen as a training ground for pathoge...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46270
更新日期:2017-04-10 00:00:00
abstract::De novo uridine-diphosphate-N-acetylglucosamine (UDP-GlcNAc) biosynthesis requires glucose, glutamine, acetyl-CoA and uridine, however GlcNAc salvaged from glycoconjugate turnover and dietary sources also makes a significant contribution to the intracellular pool. Herein we ask whether dietary GlcNAc regulates nutrien...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23043
更新日期:2016-03-14 00:00:00
abstract::Neurite growth is controlled by a complex molecular signaling network that regulates filamentous actin (F-actin) dynamics at the growth cone. The evolutionarily conserved ezrin, radixin, and moesin family of proteins tether F-actin to the cell membrane when phosphorylated at a conserved threonine residue and modulate ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20755-w
更新日期:2018-02-07 00:00:00
abstract::Broadband acoustic absorbers with thin thickness are highly desired in practical situations such as architectural acoustics, yet it is still challenging to achieve high absorption by using structure with limited thickness. Here we report the theoretical optimal design, numerical simulation and experimental demonstrati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67688-x
更新日期:2020-07-01 00:00:00
abstract::Quantifying stochastic processes is essential to understand many natural phenomena, particularly in biology, including the cell-fate decision in developmental processes as well as the genesis and progression of cancers. While various attempts have been made to construct potential landscape in high dimensional systems ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15889-2
更新日期:2017-11-17 00:00:00
abstract::The study utilizes autofluorescence spectroscopy (AFS) along with multivariate spectral analysis for differentiating various stages of hepatic fibrosis. AFS has recently emerged as an efficient tool for evaluating the variations in different endogenous flurophores. In this study, the potential of AFS for differentiati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29370-1
更新日期:2018-07-20 00:00:00
abstract::Marine sponges are early-branching, filter-feeding metazoans that usually host complex microbiomes comprised of several, currently uncultivatable symbiotic lineages. Here, we use a low-carbon based strategy to cultivate low-abundance bacteria from Spongia officinalis. This approach favoured the growth of Alphaproteoba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38737-x
更新日期:2019-02-13 00:00:00