Abstract:
:Cytometric studies utilizing flow cytometry or multi-well culture plate fluorometry are often limited by a deficit in temporal resolution and a lack of single cell consideration. Unfortunately, many cellular processes, including signaling, motility, and molecular transport, occur transiently over relatively short periods of time and at different magnitudes between cells. Here we demonstrate the multitrap nanophysiometer (MTNP), a low-volume microfluidic platform housing an array of cell traps, as an effective tool that can be used to study individual unattached cells over time with precise control over the intercellular microenvironment. We show how the MTNP platform can be used for hematologic cancer cell characterization by measuring single T cell levels of CRAC channel modulation, non-translational motility, and ABC-transporter inhibition via a calcein-AM efflux assay. The transporter data indicate that Jurkat T cells exposed to indomethacin continue to accumulate fluorescent calcein for over 60 minutes after calcein-AM is removed from the extracellular space.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Byrd TF 4th,Hoang LT,Kim EG,Pfister ME,Werner EM,Arndt SE,Chamberlain JW,Hughey JJ,Nguyen BA,Schneibel EJ,Wertz LL,Whitfield JS,Wikswo JP,Seale KTdoi
10.1038/srep05117subject
Has Abstractpub_date
2014-05-30 00:00:00pages
5117issn
2045-2322pii
srep05117journal_volume
4pub_type
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