Abstract:
:Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) phenotype in cattle, sheep, dog and human. However, there has not been reported significant muscle phenotypes in pigs in association with MSTN mutations. Pigs are an important source of meat production, as well as serve as a preferred animal model for the studies of human disease. To study the impacts of MSTN mutations on skeletal muscle growth in pigs, we generated MSTN-mutant Meishan pigs with no marker gene via zinc finger nucleases (ZFN) technology. The MSTN-mutant pigs developed and grew normally, had increased muscle mass with decreased fat accumulation compared with wild type pigs, and homozygote MSTN mutant (MSTN(-/-)) pigs had apparent DM phenotype, and individual muscle mass increased by 100% over their wild-type controls (MSTN(+/+)) at eight months of age as a result of myofiber hyperplasia. Interestingly, 20% MSTN-mutant pigs had one extra thoracic vertebra. The MSTN-mutant pigs will not only offer a way of fast genetic improvement of lean meat for local fat-type indigenous pig breeds, but also serve as an important large animal model for biomedical studies of musculoskeletal formation, development and diseases.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Qian L,Tang M,Yang J,Wang Q,Cai C,Jiang S,Li H,Jiang K,Gao P,Ma D,Chen Y,An X,Li K,Cui Wdoi
10.1038/srep14435subject
Has Abstractpub_date
2015-09-24 00:00:00pages
14435issn
2045-2322pii
srep14435journal_volume
5pub_type
杂志文章abstract::Extant Crocodylia are exceptional because they employ almost the full range of quadrupedal footfall patterns ("gaits") used by mammals; including asymmetrical gaits such as galloping and bounding. Perhaps this capacity evolved in stem Crocodylomorpha, during the Triassic when taxa were smaller, terrestrial, and long-l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55768-6
更新日期:2019-12-17 00:00:00
abstract::High-throughput RNA sequencing is a powerful tool that allows us to perform gene prediction and analyze tissue-specific overexpression of genes, but also at species level comparisons can be performed, although in a more restricted manner. In the present study complete liver transcriptomes of five tropical bat species ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42560-9
更新日期:2019-04-17 00:00:00
abstract::Recently, detecting biological particles by analyzing their mechanical properties has attracted increasing attention. To detect and identify different bioparticles and estimate their dimensions, a mechanical nanosensor is introduced in this paper. To attract particles, numerous parts of the substrate are coated with d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60296-9
更新日期:2020-02-25 00:00:00
abstract::Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08463-3
更新日期:2017-08-10 00:00:00
abstract::Chloroplast biotechnology is a route for novel crop metabolic engineering. The potential bio-confinement of transgenes, the high protein expression and the possibility to organize genes into operons represent considerable advantages that make chloroplasts valuable targets in agricultural biotechnology. In the last 3 d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78237-x
更新日期:2020-12-03 00:00:00
abstract::Reactive oxygen and nitrogen species (RONS), e.g. generated by cold physical plasma (CPP) or photodynamic therapy, interfere with redox signaling pathways of mammalian cells, inducing downstream consequences spanning from migratory impairment to apoptotic cell death. However, the more austere impact of RONS on cancer ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75514-7
更新日期:2020-10-29 00:00:00
abstract::The cause and source of the heat released from Earth's interior have not yet been determined. Some research groups have proposed that the heat is supplied by radioactive decay or by a nuclear georeactor. Here we postulate that the generation of heat is the result of three-body nuclear fusion of deuterons confined in h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37740
更新日期:2016-11-23 00:00:00
abstract::Formation-free multi-level resistive switching characteristics by using 10 nm-thick polycrystalline GeOx film in a simple W/GeOx/W structure and understanding of switching mechanism through redox reaction in H2O2/sarcosine sensing (or changing Ge°/Ge4+ oxidation states under external bias) have been reported for the f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11657-4
更新日期:2017-09-11 00:00:00
abstract::Mosquito larvae continuously encounter microbes in their aquatic environment, which serve as food and play a critical role in successful development. In previous work, we isolated a Chromobacterium sp. (C.sp_P) with larvicidal activity from the midgut of dengue vector Aedes mosquitoes in Panama. In this study, we foun...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26680-2
更新日期:2018-05-29 00:00:00
abstract::Despite the importance of urban trees, their growth reaction to climate change and to the urban heat island effect has not yet been investigated with an international scope. While we are well informed about forest growth under recent conditions, it is unclear if this knowledge can be simply transferred to urban enviro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14831-w
更新日期:2017-11-13 00:00:00
abstract::Screening and in silico modeling are critical activities for the reduction of experimental costs. They also speed up research notably and strengthen the theoretical framework, thus allowing researchers to numerically quantify the importance of a particular subset of information. For example, in fields such as cancer a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33911-z
更新日期:2018-10-24 00:00:00
abstract::Dendritic spines are the postsynaptic sites of most excitatory synapses in the brain, and spine enlargement and shrinkage give rise to long-term potentiation and depression of synapses, respectively. Because spine structural plasticity is accompanied by remodeling of actin scaffolds, we hypothesized that the filamento...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32897
更新日期:2016-09-06 00:00:00
abstract::Nucleic acid amplification tests have become a common method for diagnosis of STIs due to their improved sensitivity over immunoassays and traditional culture-based methods. Isothermal nucleic acid amplification methods offer significant advantages over polymerase chain reaction (PCR) because they do not require sophi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20487
更新日期:2016-02-03 00:00:00
abstract::The majority of oligomeric proteins form clusters which have rotational or dihedral symmetry. Despite the many advantages of symmetric packing, protein oligomers are only nearly symmetric, and the origin of this phenomenon is still in need to be fully explored. Here we apply near-symmetry analyses by the Continuous Sy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65097-8
更新日期:2020-05-20 00:00:00
abstract::Lipoprotein-associated phospholipase A2 (Lp-PLA2) levels are associated with the development of atherosclerosis. We aimed to assess the genetic determinants of Lp-PLA2 activity and mass by genotyping multiple polymorphisms in PLA2G7, the gene encoding Lp-PLA2, among 1258 participants from the Chinese Multi-provincial ...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/srep37465
更新日期:2016-12-01 00:00:00
abstract::SOCS2 is a pleiotropic E3 ligase. Its deficiency is associated with gigantism and organismal lethality upon inflammatory challenge. However, mechanistic understanding of SOCS2 function is dismal due to our unawareness of its protein substrates. We performed a mass spectrometry based proteomic profiling upon SOCS2 depl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42800
更新日期:2017-02-20 00:00:00
abstract::Unexpectedly large ocean waves or 'rogues' are sometimes claimed to be the cause of damage to ships at sea and to offshore structures. While wind-driven wave models are capable of predicting the average characteristics of waves, the maximum height of rogues that may occur is yet unknown. Rogues form in the open ocean ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44124
更新日期:2017-03-08 00:00:00
abstract::The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves. To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54360-2
更新日期:2019-11-28 00:00:00
abstract::It is highly possible that copy number variations (CNVs) in susceptible regions have effects on chronic obstructive pulmonary disease (COPD) development, while long noncoding RNA (lncRNAs) have been shown to cause COPD. We hypothesized that the common CNV, named nsv823469 located on 6p22.1, and covering lncRNAs (major...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40060
更新日期:2017-01-12 00:00:00
abstract::Changes associated with cognitive function in the high-fat high-carbohydrate diet-induced prediabetes animal model and effect of exercise remain unclear. Rats were randomly assigned to the following groups (n = 6): non-diabetic (ND), prediabetic (PD), intermittent exercising PD (PD + IE) and regular exercising PD (PD ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72098-0
更新日期:2020-09-14 00:00:00
abstract::When one liquid is introduced into another immiscible one, it ultimately fragments due to hydrodynamic instability. In contrast to neck pinch-off without external actuation, the viscous two-fluid system subjected to an oscillatory flow demonstrates higher efficiency in breaking fluid threads. However, the underlying d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31436
更新日期:2016-08-11 00:00:00
abstract::Current preclinical screening methods do not adequately detect cardiotoxicity. Using human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs), more physiologically relevant preclinical or patient-specific screening to detect potential cardiotoxic effects of drug candidates may be possible. However, one of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11817
更新日期:2015-07-03 00:00:00
abstract::The microbial community during fermented vegetable production has a large impact on the quality of the final products. Lactic acid bacteria have been well-studied in such processes, but knowledge about the roles of non-lactic acid bacteria is limited. This study aimed to provide useful knowledge about the relationship...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81105-x
更新日期:2021-01-19 00:00:00
abstract::We aimed to assess the ankle-brachial index (ABI) in patients with Graves' disease. In the cross-sectional assessments, 81 patients with drug-naïve Graves' disease and 235 with euthyroidism were enrolled. ABI and vascular cell adhesion molecule-1 (VCAM-1) levels were assessed. In the prospective follow-up, 32 patients...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74267-7
更新日期:2020-10-13 00:00:00
abstract::Although semantic processing has traditionally been associated with brain responses maximal at 350-400 ms, recent studies reported that words of different semantic types elicit topographically distinct brain responses substantially earlier, at 100-200 ms. These earlier responses have, however, been achieved using insu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01928
更新日期:2013-01-01 00:00:00
abstract::Daily music experience involves synchronizing movements in time with a perceived periodic beat. It has been established for over a century that beat synchronization is less stable for the visual than for the auditory modality. This auditory advantage of beat synchronization gives rise to the hypotheses that the neural...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11974
更新日期:2015-07-07 00:00:00
abstract::Sufficient tissue oxygenation is required for regular brain function; thus oxygen supply must be tightly regulated to avoid hypoxia and irreversible cell damage. If hypoxia occurs the transcription factor complex hypoxia-inducible factor (HIF) will accumulate and coordinate adaptation of cells to hypoxia. However, eve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75838-4
更新日期:2020-11-05 00:00:00
abstract::Natural competence for genetic transformation refers to the natural ability of various bacteria to take up exogenous DNA from their surroundings and to incorporate internalized genetic information into their genomes. By promoting bacterial diversification and adaptability, this process represents a major driving force...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47423-x
更新日期:2019-07-30 00:00:00
abstract::Despite major advances in targeted melanoma therapies, drug resistance limits their efficacy. Long noncoding RNAs (lncRNAs) are transcriptome elements that do not encode proteins but are important regulatory molecules. LncRNAs have been implicated in cancer development and response to different therapeutics and are th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27643-3
更新日期:2018-07-19 00:00:00
abstract::Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an accurate and fast method to measure gene expression. Reproducibility of the analyses is the main limitation of RT-qPCR experiments. Galaxy is an open, web-based, genomic workbench for a reproducible, transparent, and accessible scie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53155-9
更新日期:2019-11-26 00:00:00