Abstract:
:The tachykinin peptide substance P (SP) is expressed by many interneurons and some projection neurons in the superficial dorsal horn of the spinal cord. We have recently shown that SP-expressing excitatory interneurons in lamina II correspond largely to a morphological class known as radial cells. However, little is known about their function, or their synaptic connectivity. Here we use a modification of the Brainbow technique to define the excitatory synaptic input to SP radial cells. We show that around half of their excitatory synapses (identified by expression of Homer) are from boutons with VGLUT2, which are likely to originate mainly from local interneurons. The remaining synapses presumably include primary afferents, which generally have very low levels of VGLUT2. Our results also suggest that the SP cells are preferentially innervated by a population of excitatory interneurons defined by expression of green fluorescent protein under control of the gene for gastrin-releasing peptide, and that they receive sparser input from other types of excitatory interneuron. We show that around 40% of lamina I projection neurons express Tac1, the gene encoding substance P. Finally, we show that silencing Tac1-expressing cells in the dorsal horn results in a significant reduction in reflex responses to cold and radiant heat, but does not affect withdrawal to von Frey hairs, or chloroquine-evoked itch.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Polgár E,Bell AM,Gutierrez-Mecinas M,Dickie AC,Akar O,Costreie M,Watanabe M,Todd AJdoi
10.1016/j.neuroscience.2020.06.038subject
Has Abstractpub_date
2020-12-01 00:00:00pages
113-125eissn
0306-4522issn
1873-7544pii
S0306-4522(20)30417-6journal_volume
450pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Many epidemiological studies support the notion that people who drink alcohol also smoke cigarettes and vice versa thereby suggesting a possible functional interaction between these two most widely used psychoactive substances. We have earlier demonstrated that direct intracerebellar (ICB) microinfusion of nicotine do...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.08.046
更新日期:2008-11-11 00:00:00
abstract::Emerging evidence indicates that the expression of trophic factors in the brain is regulated in an activity-dependent manner, which suggests an involvement of trophic factors in events controlled by input activity. We have investigated the possibility that visual sensory input impacts the expression of basic fibroblas...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00243-7
更新日期:1999-01-01 00:00:00
abstract::The participation of excitatory neurotransmitter systems in the basal ganglia in the initiation and propagation of limbic seizures induced by pilocarpine has been investigated in the rat. Limbic seizures (electrographic and motor) occur in rats receiving bilateral microinjections into the entopeduncular nucleus of 12....
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90187-x
更新日期:1988-12-01 00:00:00
abstract::The aim of the present work was to determine whether paraventricular neurons possess functional acetylcholine nicotinic receptors. Using infrared videomicroscopy and differential interference contrast optics, we performed whole-cell recordings in hypothalamic slices containing the paraventricular nucleus. Acetylcholin...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00536-x
更新日期:2002-01-01 00:00:00
abstract::An in vitro slice preparation of combined hippocampus and entorhinal cortex from adult rats was used to study the modalities of generation and propagation, as well as the pharmacological properties of the epileptiform activity induced by the muscarinic agonist pilocarpine (10 microM). Simultaneous field potentials rec...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00534-x
更新日期:1996-05-01 00:00:00
abstract::Antisera produced in rabbits against pure fractions of cholinergic vesicles from Narcine brasiliensis were used to study cholinergic organelles in rat motor neurons. The indirect immunofluorescence method was used on perfusion-fixed material. The rats were surgically sympathectomized to remove sympathetic adrenergic a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90314-8
更新日期:1985-02-01 00:00:00
abstract::Small (SK) and intermediate (IK) conductance calcium-activated potassium channels are candidate ion channels for the regulation of excitability in nociceptive neurones. We have used unique peptide-directed antisera to describe the immunocytochemical distribution of the known isoforms of these ion channels in dorsal ro...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.09.062
更新日期:2005-01-01 00:00:00
abstract::In mice carrying the weaver mutation there is a spontaneous degeneration of dopaminergic neurons that is heterogeneous among cell groups: nigrostriatal neurons are more affected than mesolimbic neurons, while involvement of the mesocortical system is controversial. We questioned whether the pattern of cell loss in mes...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90232-1
更新日期:1994-07-01 00:00:00
abstract::The hippocampus plays an important role in the formation of new memories and spatial navigation. Recently, growing evidence supports the view that it is also involved in addiction to opiates and other drugs. Theoretical and experimental studies suggest that hippocampal neural-network oscillations at specific frequenci...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.09.052
更新日期:2010-12-15 00:00:00
abstract::Early gestation is a neurodevelopmental period that is especially vulnerable to environmental insult and one in which neurogenesis features prominently. Prenatal perturbation during early gestation has been linked to neuropsychiatric illnesses such as autism and schizophrenia, and severe environmental insult during th...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.005
更新日期:2020-08-21 00:00:00
abstract::In the present study, we first investigated the effect of single homocysteine administration on consolidation of short- and long-term memories of inhibitory avoidance task in Wistar rats. We also measured brain-derived neurotrophic factor levels in the hippocampus and parietal cortex of rats. The influence of pretreat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.07.023
更新日期:2009-11-10 00:00:00
abstract::In order to identify the specific targets of the septohippocampal projection in the rat, horseradish peroxidase localization at the electron microscopic level was used. Following injections of free horseradish peroxidase into the medial septum, sections of the dorsal hippocampal formation were reacted with diaminobenz...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90210-5
更新日期:1983-11-01 00:00:00
abstract::Tissue injury results in several changes in spinal cord neurons that contribute to hyperalgesia arising from the injured tissue. In models of unilateral hindpaw inflammation, changes in the neurochemistry and electrophysiology of dorsal horn neurons ipsilateral, and to a much lesser extent contralateral, to the inflam...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90290-i
更新日期:1992-12-01 00:00:00
abstract::Mental function has as its cerebral basis a specific dynamic structure. In particular, cortical and limbic areas involved in "higher brain functions" such as learning, memory, perception, self-awareness and consciousness continuously need to be self-adjusted even after development is completed. By this lifelong self-o...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/s0306-4522(00)00516-9
更新日期:2001-01-01 00:00:00
abstract::Both [3H]clonidine and [3H]idazoxan bind to alpha 2 adrenoceptors. The former also labels imidazoline receptors, and the latter non-adrenergic idazoxan binding sites. In order to investigate whether the imidazoline receptors and non-adrenergic idazoxan binding sites are identical, we compared the binding characteristi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90179-1
更新日期:1994-04-01 00:00:00
abstract::Hypoxia evokes a regulated decrease in the body core temperature, which response is mediated, at least in part, by noradrenaline (NA) and nitric oxide (NO) in the rostromedial preoptic area (POA) of the hypothalamus. In the accompanying paper, it was shown that glutamatergic activation of the lateral POA also evokes h...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.09.033
更新日期:2012-12-13 00:00:00
abstract::An electropharmacological analysis of the type(s) of calcium channel controlling neurotransmitter release in preganglionic sympathetic nerve terminals in the guinea-pig anterior pelvic ganglion has been carried out. Conventional intracellular recording techniques were used to record excitatory postsynaptic potentials ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00286-9
更新日期:1999-01-01 00:00:00
abstract::The granular layer of the cerebellar cortex consists of densely packed neuronal cells, classified into granule cells and large interneurons. In this study, we provide a comparative survey of large granular layer interneurons in the adult rat cerebellum based on both morphological and neurochemical criteria. To this en...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00058-6
更新日期:2001-01-01 00:00:00
abstract::We investigated computer models of a single thalamocortical neuron to assess the interaction of intrinsic voltage-sensitive channels and cortical synaptic input in producing the range of oscillation frequencies observed in these cells in vivo. A morphologically detailed model with Hodgkin-Huxley-like ion channels demo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)83006-5
更新日期:1996-02-01 00:00:00
abstract::Application of N-methyl-D-aspartate on to the dendrites of hippocampal granule cells dramatically decreased prodynorphin messenger RNA levels in the affected cells while increasing proenkephalin messenger RNA levels. Sin-1 molsidomine (an agent which releases nitric oxide) and 8-bromo-cyclic GMP were similarly effecti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90423-5
更新日期:1994-08-01 00:00:00
abstract::Transthyretin is the major thyroxine-binding protein in the plasma of rodents, and the main thyroxine-binding protein in the cerebrospinal fluid of both rodents and humans. The choroid plexus synthesizes transthyretin and secretes it to the cerebrospinal fluid. Although it was suggested that transthyretin might play a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00228-2
更新日期:2002-01-01 00:00:00
abstract::Population-based studies have demonstrated that children with a history of febrile seizure (FS) perform better than age-matched controls at hippocampus-dependent memory tasks. Here, we report that FSs induce two distinct structural reorganizations in the hippocampus and bidirectionally modify future learning abilities...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.01.011
更新日期:2016-03-24 00:00:00
abstract::Far from disproving the model of mind functioning proposed by psychoanalysis, the recent advances in neuropsychiatrical research confirmed the crucial ideas of Sigmund Freud. The hypothesis that the origin of mental illnesses lies in the impossibility for a subject to erase the long-term effects of a remote adverse ev...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2004.09.032
更新日期:2005-01-01 00:00:00
abstract::The deep cerebellar nuclei (DCN) are the final integrative units of the cerebellar network. The strongest single afferent to the DCN is formed by GABAergic Purkinje neuron axons whose synapses constitute the majority of all synapses in the DCN, with their action strongly regulating the intrinsic activity of their targ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.07.060
更新日期:2008-10-15 00:00:00
abstract::Intracellular stimulation of single propriospinal axons evoked excitatory postsynaptic potentials (EPSPs) in lumbar motoneurons. Mean EPSP amplitudes differed by two orders of magnitude when measured in different connections. After analyzing the distribution of mean amplitudes of 47 single-fiber EPSPs, two populations...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00267-6
更新日期:2001-01-01 00:00:00
abstract::GABA is the main inhibitory neurotransmitter of the CNS. It is well established that activation of GABA(A) receptors favors sleep. Three generations of hypnotics are based on these GABA(A) receptor-mediated inhibitory processes. The first and second generation of hypnotics (barbiturates and benzodiazepines respectivel...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/s0306-4522(02)00034-9
更新日期:2002-01-01 00:00:00
abstract::Synaptic vesicle protein 2 is an integral synaptic vesicle membrane glycoprotein which is present in all synapses for which it has been examined. We used an anti-synaptic vesicle protein 2 monoclonal antibody to examine synaptic vesicle protein 2 localization in the developing hamster retinofugal pathway. From postnat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00548-9
更新日期:1997-04-01 00:00:00
abstract::Alzheimer's disease (AD), the most prominent form of dementia in elderly, is a yet incurable degenerative neurological illness characterized by memory loss. Here, we used an AD rat model to investigate the in vivo efficacy of caprospinol, a disease-modifying steroid developed on the concept that reduced synthesis of 2...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.10.033
更新日期:2010-01-20 00:00:00
abstract::Diabetic peripheral neuropathy is a common complication of diabetes mellitus, and a significant proportion of individuals suffer debilitating pain that significantly affects their quality of life. Unfortunately, symptomatic treatment options have limited efficacy, and often carry significant risk of systemic adverse e...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.10.065
更新日期:2015-01-29 00:00:00
abstract::Mast cells are known for their participation in immediate and, more recently, delayed hypersensitivity reactions. They have been found in the meninges and certain brain areas where they are strictly perivascular, in close apposition to neurons, and they are activated by direct nerve stimulation or by neuropeptides. In...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90030-j
更新日期:1993-12-01 00:00:00