Abstract:
:Legionella pneumophila is a bacterial pathogen that utilises a Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential to the recruitment and delivery of effectors to the T4S machinery is the membrane-embedded T4 coupling complex (T4CC). Here, we purify an intact T4CC from the Legionella membrane. It contains the DotL ATPase, the DotM and DotN proteins, the chaperone module IcmSW, and two previously uncharacterised proteins, DotY and DotZ. The atomic resolution structure reveals a DotLMNYZ hetero-pentameric core from which the flexible IcmSW module protrudes. Six of these hetero-pentameric complexes may assemble into a 1.6-MDa hexameric nanomachine, forming an inner membrane channel for effectors to pass through. Analysis of multiple cryo EM maps, further modelling and mutagenesis provide working models for the mechanism for binding and delivery of two essential classes of Legionella effectors, depending on IcmSW or DotM, respectively.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Meir A,Macé K,Lukoyanova N,Chetrit D,Hospenthal MK,Redzej A,Roy C,Waksman Gdoi
10.1038/s41467-020-16681-zsubject
Has Abstractpub_date
2020-06-08 00:00:00pages
2864issue
1issn
2041-1723pii
10.1038/s41467-020-16681-zjournal_volume
11pub_type
杂志文章abstract::Metal-halide perovskites have been widely investigated in the photovoltaic sector due to their promising optoelectronic properties and inexpensive fabrication techniques based on solution processing. Here we report the development of inorganic CsPbBr3-based photoanodes for direct photoelectrochemical oxygen evolution ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10124-0
更新日期:2019-05-08 00:00:00
abstract::Puberty is regulated by epigenetic mechanisms and is highly sensitive to metabolic and nutritional cues. However, the epigenetic pathways mediating the effects of nutrition and obesity on pubertal timing are unknown. Here, we identify Sirtuin 1 (SIRT1), a fuel-sensing deacetylase, as a molecule that restrains female p...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06459-9
更新日期:2018-10-10 00:00:00
abstract::The main challenge for gaining biological insights from genetic associations is identifying which genes and pathways explain the associations. Here we present DEPICT, an integrative tool that employs predicted gene functions to systematically prioritize the most likely causal genes at associated loci, highlight enrich...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6890
更新日期:2015-01-19 00:00:00
abstract::Oxygen vacancies, especially their distribution, are directly coupled to the electromagnetic properties of oxides and related emergent functionalities that have implications for device applications. Here using a homoepitaxial strontium titanate thin film, we demonstrate a controlled manipulation of the oxygen vacancy ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00710-5
更新日期:2017-09-20 00:00:00
abstract::Van der Waals materials and their heterostructures offer a versatile platform for studying a variety of quantum transport phenomena due to their unique crystalline properties and the exceptional ability in tuning their electronic spectrum. However, most experiments are limited to devices that have lateral dimensions o...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11379-3
更新日期:2019-07-26 00:00:00
abstract::Fire emissions generate air pollutants ozone (O3) and aerosols that influence the land carbon cycle. Surface O3 damages vegetation photosynthesis through stomatal uptake, while aerosols influence photosynthesis by increasing diffuse radiation. Here we combine several state-of-the-art models and multiple measurement da...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07921-4
更新日期:2018-12-21 00:00:00
abstract::The cost, size and availability of electron accelerators are dominated by the achievable accelerating gradient. Conventional high-brightness radio-frequency accelerating structures operate with 30-50 MeV m(-1) gradients. Electron accelerators driven with optical or infrared sources have demonstrated accelerating gradi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9486
更新日期:2015-10-06 00:00:00
abstract::Individual cells in clonal populations often respond differently to environmental changes; for binary phenotypes, such as cell death, this can be measured as a fractional response. These types of responses have been attributed to cell-intrinsic stochastic processes and variable abundances of biochemical constituents, ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09275-x
更新日期:2019-03-21 00:00:00
abstract::Zero-energy particles (such as Majorana fermions) are newly predicted quasiparticles and are expected to play an important role in fault-tolerant quantum computation. In conventional Hermitian quantum systems, however, such zero states are vulnerable and even become vanishing if couplings with surroundings are of the ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03822-8
更新日期:2018-04-03 00:00:00
abstract::Integrin αVβ8, which like αVβ6 functions to activate TGF-βs, is atypical. Its β8 subunit binds to a distinctive cytoskeleton adaptor and does not exhibit large changes in conformation upon binding to ligand. Here, crystal structures, hydrogen-deuterium exchange dynamics, and affinity measurements on mutants are used t...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13248-5
更新日期:2019-12-02 00:00:00
abstract::The diversity in our genome is crucial to understanding the demographic history of worldwide populations. However, we have yet to know whether subtle genetic differences within a population can be disentangled, or whether they have an impact on complex traits. Here we apply dimensionality reduction methods (PCA, t-SNE...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15194-z
更新日期:2020-03-26 00:00:00
abstract::Traditionally, molecular assembly pathways for viruses are inferred from high resolution structures of purified stable intermediates, low resolution images of cell sections and genetic approaches. Here, we directly visualise an unsuspected 'single shelled' intermediate for a mammalian orthoreovirus in cryo-preserved i...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18243-9
更新日期:2020-09-07 00:00:00
abstract::In adult skin epidermis and the epithelium lining the esophagus cells are constantly shed from the tissue surface and replaced by cell division. Tracking genetically labelled cells in transgenic mice has given insight into cell behavior, but conflicting models appear consistent with the results. Here, we use an additi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15258-0
更新日期:2020-03-18 00:00:00
abstract::Adaptive behavior requires integrating prior with current information to anticipate upcoming events. Brain structures related to this computation should bring relevant signals from the recent past into the present. Here we report that rats can integrate the most recent prior information with sensory information, there...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14823
更新日期:2017-03-24 00:00:00
abstract::Intracellular trafficking of organelles, driven by kinesin-1 stepping along microtubules, underpins essential cellular processes. In absence of other proteins on the microtubule surface, kinesin-1 performs micron-long runs. Under crowding conditions, however, kinesin-1 motility is drastically impeded. It is thus uncle...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16972-5
更新日期:2020-06-19 00:00:00
abstract::The plausibility of any model in science comes from the extent of its interconnections to other models that are grounded in different premises and reasoning. Focusing research on paradoxes in those models, logic whereby they appear to generate unacceptable conclusions from seemingly indisputable premises, helps find t...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07274-y
更新日期:2018-12-12 00:00:00
abstract::A primary function of the midbrain stimulus selection network is to compute the highest-priority location for attention and gaze. Here we report the contribution of a specific cholinergic circuit to this computation. We functionally disconnected the tegmental cholinergic nucleus isthmi pars parvocellularis (Ipc) from ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13472
更新日期:2016-11-17 00:00:00
abstract::Biodegradable polyesters with various tacticities have been synthesized by means of stereoselective ring-opening polymerization of racemic lactide and β-lactones but with limited side-chain groups. However, stereoselective synthesis of functional polyesters remains challenging from O-carboxyanhydrides that have abunda...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03879-5
更新日期:2018-04-19 00:00:00
abstract::Clustered regularly-interspaced palindromic repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful to identify genetic regulators. Applying CRISPR screens to interrogate functional elements in noncoding regions requires generating sgRNA libraries that are densely covering,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11178
更新日期:2016-03-30 00:00:00
abstract::Chaperone-mediated autophagy (CMA) is a selective mechanism for the degradation of soluble proteins in lysosomes. CMA contributes to cellular quality control and is activated as part of the cellular response to different stressors. Defective CMA has been identified in ageing and different age-related diseases. Until n...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1393
更新日期:2011-07-12 00:00:00
abstract::Fusion proteins containing blue-light-activable protein domains possess great potential as molecular switches in cell signalling. This has recently been impressively demonstrated by connecting the light oxygen voltage LOV2-Jα-protein domain of A. sativa (AsLOV2-Jα) with the Rac1-GTPase, responsible for regulating the ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1121
更新日期:2010-11-16 00:00:00
abstract::The Unc-51 like autophagy activating kinase 1 (ULK1) complex plays a central role in the initiation stage of autophagy. However, the function of ULK1 in the late stage of autophagy is unknown. Here, we report that ULK1, a central kinase of the ULK1 complex involved in autophagy initiation, promotes autophagosome-lysos...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05449-1
更新日期:2018-08-28 00:00:00
abstract::Exploitation of drug-drug synergism and allostery could yield superior therapies by capitalizing on the immensely diverse, but highly specific, potential associated with the biological macromolecular landscape. Here we describe a drug-drug synergy mediated by allosteric cross-talk in chromatin, whereby the binding of ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14860
更新日期:2017-03-30 00:00:00
abstract::Island systems generally have fewer species than continental areas due to their small size and geographical isolation. Low island diversity reduces the possibility of exportation of island lineages and island systems are not thought to have a major influence on the build-up of continental diversity. However, the view ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9538
更新日期:2015-10-05 00:00:00
abstract::How are memories transferred from short-term to long-term storage? Systems-level memory consolidation is thought to be dependent on the coordinated interplay of cortical slow waves, thalamo-cortical sleep spindles and hippocampal ripple oscillations. However, it is currently unclear how the selective interaction of th...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11444-x
更新日期:2019-08-08 00:00:00
abstract::Sirtuins regulate numerous important biological processes in mammals, including various age-associated pathophysiologies. However, whether sirtuins are critical to control ageing and longevity in mammals has been controversial. Recent studies have demonstrated critical roles of sirtuins in the brain, especially the hy...
journal_title:Nature communications
pub_type: 杂志文章,评审
doi:10.1038/ncomms5211
更新日期:2014-06-26 00:00:00
abstract::The intestinal microbiome is a unique ecosystem and an essential mediator of metabolism and obesity in mammals. However, studies investigating the impact of the diet on the establishment of the gut microbiome early in life are generally lacking, and most notably so in primate models. Here we report that a high-fat mat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4889
更新日期:2014-05-20 00:00:00
abstract::The vertebrate mineralized skeleton is known to have first emerged as an exoskeleton that extensively covered the fossil jawless fish. The evolutionary origin of this exoskeleton has long been attributed to the emergence of the neural crest, but experimental evaluation for this is still poor. Here we determine the emb...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2643
更新日期:2013-01-01 00:00:00
abstract::The development of drug resistance, the prime cause of failure in cancer therapy, is commonly explained by the selection of resistant mutant cancer cells. However, dynamic non-genetic heterogeneity of clonal cell populations continuously produces metastable phenotypic variants (persisters), some of which represent ste...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3467
更新日期:2013-01-01 00:00:00
abstract::Coalescence is an essential phenomenon that governs the equilibrium behaviour in a variety of systems from intercellular transport to planetary formation. In this report, we study coalescence pathways of circularly shaped two-dimensional colloidal membranes, which are one rod-length-thick liquid-like monolayers of ali...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4063
更新日期:2014-01-01 00:00:00