Abstract:
:Increased glucose uptake and aerobic glycolysis are striking features of many cancers. These features have led to many techniques for screening and diagnosis, but many are expensive, less feasible or have harmful side-effects. Here, we report a sensitive 1H/2H NMR method to measure the kinetics of lactate isotopomer and HDO production using a deuterated tracer. To test this hypothesis, HUH-7 hepatocellular carcinoma and AML12 normal hepatocytes were incubated with [2H7]glucose. 1H/2H NMR data were recorded for cell media as a function of incubation time. The efflux rate of lactate-CH3, lactate-CH2D and lactate-CHD2 was calculated as 0.0033, 0.0071, and 0.0.012 µmol/106cells/min respectively. Differential production of lactate isotopomers was due to deuterium loss during glycolysis. Glucose uptake and HDO production by HUH-7 cells showed a strong correlation, indicating that monitoring the HDO production could be a diagnostic feature in cancers. Deuterium mass balance of [2H7]glucose uptake to 2H-lactate and HDO production is quantitatively matched, suggesting increasing HDO signal could be used to diagnose Warburg (cancer) metabolism. Measuring the kinetics of lactate isotopomer and HDO production by 1H and 2H MR respectively are highly sensitive. Increased T1 of 2H-lactate isotopomers indicates inversion/saturation recovery methods may be a simple means of generating metabolism-based contrast.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mahar R,Donabedian PL,Merritt MEdoi
10.1038/s41598-020-65839-8subject
Has Abstractpub_date
2020-06-01 00:00:00pages
8885issue
1issn
2045-2322pii
10.1038/s41598-020-65839-8journal_volume
10pub_type
杂志文章abstract::Expression profiling of multiple microRNAs (miRNAs) generally provides valuable information for understanding various biological processes. Thus, it is necessary to develop a sensitive and accurate miRNA assay suitable for multiplexing. Isothermal exponential amplification reaction (EXPAR) has received significant int...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11895-6
更新日期:2017-09-12 00:00:00
abstract::A mathematical model has been developed to examine the magneto hydrodynamic micropolar nanofluid flow with buoyancy effects. Flow analysis is carried out in the presence of nonlinear thermal radiation and dual stratification. The impact of binary chemical reaction with Arrhenius activation energy is also considered. A...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13140-6
更新日期:2017-10-10 00:00:00
abstract::Most male lepidopterans produce fertile eupyrene sperm and non-fertile apyrene sperm, both of which are transferred to the female in a spermatophore during mating. Apyrene sperm outnumbers eupyrene sperm and both sperm types migrate from the bursa copulatrix to the spermatheca after mating. While eupyrene sperm are ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77683-x
更新日期:2020-12-01 00:00:00
abstract::Coherent B2-ordered NiAl-type precipitates have been used to reinforce solid-solution body-centered-cubic iron for high-temperature application in fossil-energy power plants. In this study, we investigate the stability of nano-sized precipitates in a NiAl-strengthened ferritic alloy at 700-950 °C using ultra-small ang...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16081
更新日期:2015-11-05 00:00:00
abstract::We present a combined geomorphological and biochronological study aimed at providing age constraints to the deposits forming a wide paleo-surface in the coastal area of the Tyrrhenian Sea, south of Anzio promontory (central Italy). We review the faunal assemblage recovered in Campoverde, evidencing the occurrence of t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36111-x
更新日期:2018-12-13 00:00:00
abstract::Activity-regulated cytoskeleton-associated protein (Arc; also known as Arg3.1) is an immediate early gene product that is transcribed in dendritic spines and, to date, has been best characterized as a positive regulator of AMPAR endocytosis during long-term depression (LTD) through interaction with endocytic proteins....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32821-4
更新日期:2018-10-02 00:00:00
abstract::Savant syndrome is a phenomenon whereby individuals with cognitive impairments have one or more outstanding abilities, inconsistent with their general intellectual functioning. Approximately 50% of savant individuals have autism spectrum disorder (ASD), and 10-30% of people with ASD have savant skills. To shed additio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59209-7
更新日期:2020-02-10 00:00:00
abstract::2,5-Dimethylfuran (DMF) is a type of attractive sustainable green energy for diesel engines that is designed to reduce soot emission. This study investigated the effect of fuel properties on the macroscopic and microscopic spray characteristics of four test blends under injection pressures of 90, 120 and 150 MPa and a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58119-y
更新日期:2020-01-29 00:00:00
abstract::Demand for clinical decision support systems in medicine and self-diagnostic symptom checkers has substantially increased in recent years. Existing platforms rely on knowledge bases manually compiled through a labor-intensive process or automatically derived using simple pairwise statistics. This study explored an aut...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05778-z
更新日期:2017-07-20 00:00:00
abstract::The relative effects of internal fixation strategies for intertrochanteric fracture after operation remain uncertain. We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) to address this important issue. We searched PubMed, EMBASE and CENTRAL for RCTs that compared different intern...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析,评审
doi:10.1038/srep18195
更新日期:2015-12-11 00:00:00
abstract::Recent severe European droughts raise the vital question: are we already experiencing measurable changes in drought likelihood that agree with climate change projections? The plethora of drought definitions compounds this question, requiring instead that we ask: how have various types of drought changed, how do these ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14283-2
更新日期:2017-10-25 00:00:00
abstract::Nanomedicine development currently suffers from a lack of efficient tools to predict pharmacokinetic behavior without relying upon testing in large numbers of animals, impacting success rates and development costs. This work presents dendPoint, the first in silico model to predict the intravenous pharmacokinetics of d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51789-3
更新日期:2019-10-29 00:00:00
abstract::This study presents analysis of four chimney samples in terms of glycerol dialkyl glycerol tetraether lipids (GDGTs), representing different growing stages of sulfide chimneys at the Deyin hydrothermal field, the southern mid-Atlantic ridge. The modified Bligh-Dyer method was used for lipid extraction and purification...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26166-1
更新日期:2018-05-23 00:00:00
abstract::Many insects possess the ability to detect fine fluctuations in the environmental CO2 concentration. In herbivorous species, plant-emitted CO2, in combination with other sensory cues, affect many behaviors including foraging and oviposition. In contrast to the comprehensive knowledge obtained on the insect olfactory p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76918-1
更新日期:2020-11-16 00:00:00
abstract::The aim of this study was to determine the association of residual feed intake (RFI) with growth performance, blood metabolic parameters, and body composition factors in growing lambs. Individual body weight (BW) and dry matter intake (DMI) were determined in 137 male Hu lambs that were given a pellet feed four times ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13042-7
更新日期:2017-10-04 00:00:00
abstract::The aim of this study was to estimate substitution rate and imprints of natural selection on parvovirus B19 genotype 1. Studied datasets included 137 near complete coding B19 genomes (positions 665 to 4851) for phylogenetic and substitution rate analysis and 146 and 214 partial genomes for selection analyses in open r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35759
更新日期:2016-10-24 00:00:00
abstract::The pathophysiological mechanism involved in side effects of radiation therapy, and especially the role of the endothelium remains unclear. Previous results showed that plasminogen activator inhibitor-type 1 (PAI-1) contributes to radiation-induced intestinal injury and suggested that this role could be driven by an e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15738
更新日期:2015-10-29 00:00:00
abstract::The mosquito, Aedes baisasi, which inhabits brackish mangrove swamps, is known to feed on fish. However, its host assemblage has not been investigated at the species level. We amplified and sequenced the cytochrome oxidase subunit I barcoding regions as well as some other regions from blood-fed females to identify hos...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40509-6
更新日期:2019-03-08 00:00:00
abstract::The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39118
更新日期:2016-12-20 00:00:00
abstract::Ferritin has gained significant attention as a potential reporter gene for in vivo imaging by magnetic resonance imaging (MRI). However, due to the ferritin ferrihydrite core, the relaxivity and sensitivity for detection of native ferritin is relatively low. We report here on a novel chimeric magneto-ferritin reporter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26550
更新日期:2016-05-23 00:00:00
abstract::The purpose of this paper is to develop a lightweight apron that will be used for shielding low intensity radiation in medical imaging radiography room and to apply it to a custom-made effective shielding. The quality of existing aprons made for protecting our bodies from direct radiation are improved so that they are...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27721
更新日期:2016-07-27 00:00:00
abstract::This corrects the article DOI: 10.1038/srep44888. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep46881
更新日期:2017-08-29 00:00:00
abstract::Air-core magnetometers are amongst the most commonly used magnetic field detectors in biomedical instruments. They offer excellent sensitivity, low fabrication complexity and a robust, cost-effective solution. However, air-core magnetometers must be tailored to the specific application to achieve high sensitivity, whi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02099-z
更新日期:2017-05-23 00:00:00
abstract::Cervical sagittal alignment is considered to have a special role in the pathogenesis and evaluation of cervical diseases. Previous studies have demonstrated that cervical sagittal alignment is correlated with thoracolumbar and pelvic alignments. As the direct anatomical structures connect to the cervical region, howev...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33287-0
更新日期:2018-10-18 00:00:00
abstract::Clinical differentiation between cystic lesions of endodontic and non-endodontic origin is of importance because correct diagnosis may affect treatment decision making. Most radicular cysts are treated with conservative approaches and, therefore, are not surgically removed. The objective of this study was to determine...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71029-3
更新日期:2020-08-25 00:00:00
abstract::It is now well established that invasive plants may induce drifts in the quantity and/or quality of floral resources. They are then often pointed out as a potential driver of bee decline. However, their impact on bee population remains quite unclear and still controversial, as bee responses are highly variable among s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16054-5
更新日期:2017-11-24 00:00:00
abstract::Sub-retinal fluid (SRF) has been discussed as a protective factor against macular atrophy in eyes with neovascular age-related macular degeneration (nAMD).To gauge the impact of SRF on macular atrophy, a database of 310 nAMD eyes was screened for eyes manifesting an SRF-only phenotype under treat & extend anti-VEGF tr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64901-9
更新日期:2020-05-15 00:00:00
abstract::Endovascular methods have been increasingly applied in treating cervicocranial artery dissection (CCAD). Anti-thrombotic therapy, which is used in non-interventional care of CCAD patients, has differential effects in East Asian patients. Therefore, we aimed to compare the clinical outcomes of endovascular versus non-i...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析,评审
doi:10.1038/srep10474
更新日期:2015-05-20 00:00:00
abstract::Arctic river watersheds are important components of the global climate system and show an amplified response to climate change. Here, we characterize origins and transformations of dissolved organic matter (DOM) in five major Arctic rivers (Kolyma, Lena, Yenisei, Ob, Mackenzie) over 3 years with seasonal sampling peri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12729-1
更新日期:2017-10-19 00:00:00
abstract::The intravenous, intramuscular or intraperitoneal administration of water solubilized graphene nanoparticles for biomedical applications will result in their interaction with the hematological components and vasculature. Herein, we have investigated the effects of dextran functionalized graphene nanoplatelets (GNP-Dex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02584
更新日期:2013-01-01 00:00:00