Abstract:
:The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure of the CR from Xenopus laevis NPC at average resolutions of 5.5-7.9 Å, with local resolutions reaching 4.5 Å. Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components. The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits, forming a circular scaffold. Within each CR subunit, the Nup358-containing region wraps around the stems of both Y complexes, likely stabilizing the scaffold. Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighboring Y complex. The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC. These previously uncharacterized structural features reveal insights into NPC assembly.
journal_name
Cell Resjournal_title
Cell researchauthors
Huang G,Zhang Y,Zhu X,Zeng C,Wang Q,Zhou Q,Tao Q,Liu M,Lei J,Yan C,Shi Ydoi
10.1038/s41422-020-0319-4subject
Has Abstractpub_date
2020-06-01 00:00:00pages
520-531issue
6eissn
1001-0602issn
1748-7838pii
10.1038/s41422-020-0319-4journal_volume
30pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract::MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that target protein-coding mRNAs at the post-transcriptional level. Our previous studies suggest that mir-21 functions as an oncogene and has a role in tumorigenesis, in part through regulation of the tumor suppressor gene tropomyosin 1 (TPM1)...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2008.24
更新日期:2008-03-01 00:00:00
abstract::Apoptosis is a complex process involving a large array of genes and mutation of any of these genes may lead to malignancy formation. Re-acquirement of FasL by tumor cells may enable them to evade the surveillance of immune system and thus contributes to the growth of tumor. Apart from traditional therapies, inducing a...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290060
更新日期:2001-03-01 00:00:00
abstract::DNA methylation and demethylation regulate many crucial biological processes in mammals and are linked to many diseases. Active DNA demethylation is believed to be catalyzed by TET proteins and a putative DNA decarboxylase that may share some similarities in sequence, structure and catalytic mechanism with isoorotate ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2013.107
更新日期:2013-11-01 00:00:00
abstract::A paracrine regulation was recently proposed in human embryonic stem cells (hESCs) grown in mouse embryonic fibroblast (MEF)-conditioned media (MEF-CM), where hESCs spontaneously differentiate into autologous fibroblast-like cells to maintain culture homeostasis by producing TGF-beta and insulin-like growth factor-II ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2009.35
更新日期:2009-06-01 00:00:00
abstract::DNA damage can, but does not always, induce cell death. While several pathways linking DNA damage signals to mitochondria-dependent and -independent death machineries have been elucidated, the connectivity of these pathways is subject to regulation by multiple other factors that are not well understood. We have propos...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2007.110
更新日期:2008-01-01 00:00:00
abstract::Pluripotent stem cells (PSCs) harbor constitutive DNA replication stress during their rapid proliferation and the consequent genome instability hampers their applications in regenerative medicine. It is therefore important to understand the regulatory mechanisms of replication stress response in PSCs. Here, we report ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.139
更新日期:2018-01-01 00:00:00
abstract::Toll-Like Receptors (TLRs) play critical roles in the early innate immune response to invading pathogens by sensing microorganisms; a number of accessory molecules have been shown to assist microbial recognition by TLRs. In a recent paper in Cell Research, Yang et al. demonstrate that Mex3B is associated with TLR3 in ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.29
更新日期:2016-04-01 00:00:00
abstract::SRC-3/AIB1 (steroid receptor coactivator 3/amplified in breast cancer 1) is an authentic oncogene that contributes to the development of drug resistance and poor disease-free survival in cancer patients. Autophagy is also an important cell death mechanism that has tumor suppressor function. In this study, we identifie...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2012.44
更新日期:2012-06-01 00:00:00
abstract::Achieving increased grain productivity has long been the overriding focus of cereal breeding programs. The ideotype approach has been used to improve rice yield potential at the International Rice Research Institute and in China. However, the genetic basis of yield-related traits in rice remains unclear. Here, we show...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.98
更新日期:2017-09-01 00:00:00
abstract::Breast cancer is a heterogeneous disease. In particular, triple-negative breast cancer (TNBC) comprises various molecular subgroups with unclear identities and currently has few targeted treatment options. Our previous study identified protein C receptor (Procr) as a surface marker on mammary stem cells (MaSCs) locate...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-019-0225-9
更新日期:2019-10-01 00:00:00
abstract::One of the two X chromosomes in cells of female mammals is transcriptionally silenced in a process known as X chromosome inactivation (XCI). Initiation of XCI is regulated by the ubiquitin ligase Rnf12/RLIM, but the mechanisms by which Rnf12/RLIM mediates this process has been a mystery. A recent study by Gontan et al...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2012.98
更新日期:2012-11-01 00:00:00
abstract::Inflammasomes are multi-component signaling complexes critical to the initiation of pyroptotic cell death in response to invading pathogens and cellular damage. A number of innate immune receptors have been reported to serve as inflammasome sensors. Activation of these sensors leads to the proteolytic activation of ca...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-020-0295-8
更新日期:2020-04-01 00:00:00
abstract::In vivo genome editing represents a powerful strategy for both understanding basic biology and treating inherited diseases. However, it remains a challenge to develop universal and efficient in vivo genome-editing tools for tissues that comprise diverse cell types in either a dividing or non-dividing state. Here, we d...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-019-0213-0
更新日期:2019-10-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Cell research
pub_type: 已发布勘误
doi:10.1038/s41422-019-0262-4
更新日期:2020-02-01 00:00:00
abstract::Fishes, the biggest and most diverse community in vertebrates are good experimental models for studies of cell and developmental biology by many favorable characteristics. Nuclear transplantation in fish has been thoroughly studied in China since 1960s. Fish nuclei of embryonic cells from different genera were transpl...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290032
更新日期:2000-03-01 00:00:00
abstract::Ferroptosis is an iron-dependent form of regulated necrosis. It is implicated in various human diseases, including ischemic organ damage and cancer. Here, we report the crucial role of autophagy, particularly autophagic degradation of cellular iron storage proteins (a process known as ferritinophagy), in ferroptosis. ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.95
更新日期:2016-09-01 00:00:00
abstract::Upon encountering the antigen (Ag), the immune system can either develop a specific immune response or enter a specific state of unresponsiveness, tolerance. The response of B cells to their specific Ag can be activation and proliferation, leading to the immune response, or anergy and activation-induced cell death (AI...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290047
更新日期:2000-09-01 00:00:00
abstract::Technologies allowing for specific regulation of endogenous genes are valuable for the study of gene functions and have great potential in therapeutics. We created the CRISPR-on system, a two-component transcriptional activator consisting of a nuclease-dead Cas9 (dCas9) protein fused with a transcriptional activation ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2013.122
更新日期:2013-10-01 00:00:00
abstract::Postzygotic single-nucleotide mutations (pSNMs) have been studied in cancer and a few other overgrowth human disorders at whole-genome scale and found to play critical roles. However, in clinically unremarkable individuals, pSNMs have never been identified at whole-genome scale largely due to technical difficulties an...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2014.131
更新日期:2014-11-01 00:00:00
abstract::The authors apologized for the spelling mistake of author name for Zhengyu Ju. The correct form is Zhenyu Ju. We apologize for any hassle that may have been caused by our mistake. ...
journal_title:Cell research
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41422-018-0063-1
更新日期:2018-08-01 00:00:00
abstract::In order to analyze the mechanism of immuno-modulation by LPS on murine peritoneal suppressor macrophages, we have, using RNase protection assay, checked the changes of mRNA expression pattern of several cytokine genes during the immuno-modulation. It has been found that, after treating peritoneal suppressor macrophag...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.1998.32
更新日期:1998-12-01 00:00:00
abstract::Aging is associated with many complex diseases. Reliable prediction of the aging process is important for assessing the risks of aging-associated diseases. However, despite intense research, so far there is no reliable aging marker. Here we addressed this problem by examining whether human 3D facial imaging features c...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2015.36
更新日期:2015-05-01 00:00:00
abstract::Wnt/β-catenin signaling is essential for the initiation of dorsal-ventral patterning during vertebrate embryogenesis. Maternal β-catenin accumulates in dorsal marginal nuclei during cleavage stages, but its critical target genes essential for dorsalization are silent until mid-blastula transition (MBT). Here, we find ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.141
更新日期:2017-02-01 00:00:00
abstract::Various systems by using electric pulse, calcium, or polyethylene glycol have been developed in the past decade for the in vitro fusion of plant gametes. These in vitro systems provide a new way to study the fertilization mechanisms of plants. In this study, we developed a bovine serum albumin (BSA)-mediated fusion sy...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290343
更新日期:2005-09-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Cell research
pub_type: 已发布勘误
doi:10.1038/s41422-020-0311-z
更新日期:2020-06-01 00:00:00
abstract::The family members of the mitogen-activated protein (MAP) kinases mediate a wide variety of cellular behaviors in response to extracellular stimuli. One of the four main sub-groups, the p38 group of MAP kinases, serve as a nexus for signal transduction and play a vital role in numerous biological processes. In this re...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290257
更新日期:2005-01-01 00:00:00
abstract::Parthenogenetic embryonic stem (pES) cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. However, the pluripotency of pES cells and the expression profiles of paternally expressed imprinted genes have not been fully explored. In this study, three mouse...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2007.70
更新日期:2007-09-01 00:00:00
abstract::The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin, is a central cell growth regulating kinase that forms large molecular complexes in all eukaryotic cells. A paper recently published in Science reports the architecture of mTOR complex 1 (mTORC1) and provides molecular insights...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.10
更新日期:2016-03-01 00:00:00
abstract::Tumor necrosis factor (TNF) family ligands play essential roles in regulating a variety of cellular processes including proliferation, differentiation and survival. Expression of Drosophila TNF ortholog Eiger (Egr) induces JNK-dependent cell death, while the roles of caspases in this process remain elusive. To further...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.135
更新日期:2012-02-01 00:00:00
abstract::Leaf morphogenesis requires the establishment of adaxial-abaxial polarity after primordium initiation from the shoot apical meristem (SAM). Several families of transcription factors are known to play critical roles in promoting adaxial or abaxial leaf fate. Recently, post-transcriptional gene silencing pathways have b...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2007.45
更新日期:2007-06-01 00:00:00