Abstract:
:TRAF-interacting protein with a forkhead-associated (FHA) domain (TIFA), originally identified as an adaptor protein of TRAF6, has recently been shown to be involved in innate immunity, induced by a pathogen-associated molecular pattern (PAMP). ADP-β-D-manno-heptose, a newly identified PAMP, binds to alpha-kinase 1 (ALPK1) and activates its kinase activity to phosphorylate TIFA. Phosphorylation triggers TIFA oligomerisation and formation of a subsequent TIFA-TRAF6 oligomeric complex for ubiquitination of TRAF6, eventually leading to NF-κB activation. However, the structural basis of TIFA-dependent TRAF6 signalling, especially oligomer formation of the TIFA-TRAF6 complex remains unknown. In the present study, we determined the crystal structures of mouse TIFA and two TIFA mutants-Thr9 mutated to either Asp or Glu to mimic the phosphorylation state-to obtain the structural information for oligomer formation of the TIFA-TRAF6 complex. Crystal structures show the dimer formation of mouse TIFA to be similar to that of human TIFA, which was previously reported. This dimeric structure is consistent with the solution structure obtained from small angle X-ray scattering analysis. In addition to the structural analysis, we examined the molecular assembly of TIFA and the TIFA-TRAF6 complex by size-exclusion chromatography, and suggested a model for the TIFA-TRAF6 signalling complex.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nakamura T,Hashikawa C,Okabe K,Yokote Y,Chirifu M,Toma-Fukai S,Nakamura N,Matsuo M,Kamikariya M,Okamoto Y,Gohda J,Akiyama T,Semba K,Ikemizu S,Otsuka M,Inoue JI,Yamagata Ydoi
10.1038/s41598-020-61972-6subject
Has Abstractpub_date
2020-03-20 00:00:00pages
5152issue
1issn
2045-2322pii
10.1038/s41598-020-61972-6journal_volume
10pub_type
杂志文章abstract::There is increasing evidence that extracellular cGMP modulates glutamatergic neurotransmission and some forms of learning. However, the underlying mechanisms remain unknown. We proposed the hypotheses that extracellular cGMP may regulate membrane expression of AMPA receptors. To do this extracellular cGMP should act o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18024-3
更新日期:2017-12-15 00:00:00
abstract::In spite of much work on topological insulators (TIs), systematic experiments for TI/TI heterostructures remain absent. We grow a high quality heterostructure containing single quintuple layer (QL) of Bi2Se3 on 19 QLs of Bi2Te3 and compare its transport properties with 20 QLs Bi2Se3 and 20 QLs Bi2Te3. All three films ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03060
更新日期:2013-10-28 00:00:00
abstract::Metamagnetism occuring inside a ferromagnetic phase is peculiar. Therefore, Sr4Ru3O10, a T C = 105 K ferromagnet, has attracted much attention in recent years, because it develops a pronounced metamagnetic anomaly below T C for magnetic fields applied in the crystallographic ab-plane. The metamagnetic transition move...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03648-2
更新日期:2017-06-20 00:00:00
abstract::Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, revealed by increased accumul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02643-x
更新日期:2017-05-30 00:00:00
abstract::The Killari Earthquake (Moment magnitude 6.1) of September 30, 1993, occurred in the state of Maharashtra, India, has an epicenter (18°03' N, 76°33' E) located at ~ 40 km SSW of Killari Town. The ~ 125 km long basin of Tirna River, close to the Killari Town, currently occupies the area that has witnessed episodic intr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77542-9
更新日期:2020-11-25 00:00:00
abstract::With Motor imagery (MI), movements are mentally rehearsed without overt actions; this procedure has been adopted in motor rehabilitation, primarily in brain-damaged patients. Here we rather tested the clinical potentials of MI in purely orthopaedic patients who, by definition, should maximally benefit of mental exerci...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65820-5
更新日期:2020-06-02 00:00:00
abstract::Magnesium-based thermoelectric materials (Mg2X, X = Si, Ge, Sn) have received considerable attention due to their availability, low toxicity, and reasonably good thermoelectric performance. The synthesis of these materials with high purity is challenging, however, due to the reactive nature and high vapour pressure of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04348-7
更新日期:2017-06-21 00:00:00
abstract::Amiodarone accumulates in the liver, where it increases x-ray attenuation due to its iodine content. We evaluated liver attenuation in patients treated and not treated with amiodarone using true-non-contrast (TNC) and virtual-non-contrast (VNC) images acquired with spectral-detector-CT (SDCT). 142 patients, of which 2...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64002-7
更新日期:2020-04-24 00:00:00
abstract::The interaction between roots and bacterial communities in halophytic species is poorly understood. Here, we used Jerusalem artichoke cultivar Nanyu 1 (NY-1) to characterise root distribution patterns and determine diversity and abundance of bacteria in the rhizosphere soil under variable salinity. Root growth was not...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20687
更新日期:2016-02-08 00:00:00
abstract::Multi-junction solar cells made by assembling semiconductor materials with different bandgap energies have hold the record conversion efficiencies for many years and are currently approaching 50%. Theoretical efficiency limits make use of optimum designs with the right lattice constant-bandgap energy combination, whic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04321-4
更新日期:2017-06-21 00:00:00
abstract::Advances in genomics have the potential to revolutionize clinical diagnostics. Here, we examine the microbiome of vitreous (intraocular body fluid) from patients who developed endophthalmitis following cataract surgery or intravitreal injection. Endophthalmitis is an inflammation of the intraocular cavity and can lead...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22416-4
更新日期:2018-03-07 00:00:00
abstract::Haze is a serious issue in China with increasing concerns, and understanding the factors driving decadal-scale variations in haze occurrence is relevant for government policymaking. Using a comprehensive observational haze dataset, we demonstrate notable decadal fluctuations in the number of haze days (HD) during wint...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27424
更新日期:2016-06-10 00:00:00
abstract::Previous studies have demonstrated that patients with posttraumatic stress disorder (PTSD) caused by different types of trauma may show divergence in epidemiology, clinical manifestation and treatment outcome. However, it is still unclear whether this divergence has neuroanatomic correlates in PTSD brains. To elucidat...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析,评审
doi:10.1038/srep33748
更新日期:2016-09-21 00:00:00
abstract::Active video games (exergames) may provide short-term increase in energy expenditure. We explored the effects of gender and prior experience on aerobic and anaerobic energy systems contributions, and the activity profiles of 40 participants playing with a swimming exergame. We recorded oxygen consumption and assessed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05583-8
更新日期:2017-07-12 00:00:00
abstract::Finding overlapping communities of complex networks remains a challenge in network science. To address this challenge, one of the widely used approaches is finding the communities of links by optimizing the objective function, partition density. In this study, we show that partition density suffers from inverse resolu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12432-1
更新日期:2017-09-29 00:00:00
abstract::Nociceptive neurons play an essential role in pain sensation by transmitting painful stimuli to the central nervous system. However, investigations of nociceptive neuron biology have been hampered by the lack of accessibility of human nociceptive neurons. Here, we describe a system for efficiently guiding human embryo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16821
更新日期:2015-11-19 00:00:00
abstract::The exposure of aqueous solutions to atmospheric plasmas results in the generation of relatively long-lived secondary products such as hydrogen peroxide which are biologically active and have demonstrated anti-microbial and cytotoxic activity. The use of plasma-activated solutions in applications such as microbial dec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21464
更新日期:2016-02-24 00:00:00
abstract::Human aldose reductase (hAR, AKR1B1) has been explored as drug target since the 1980s for its implication in diabetic complications. An activated form of hAR was found in cells from diabetic patients, showing a reduced sensitivity to inhibitors in clinical trials, which may prevent its pharmacological use. Here we rep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39722-0
更新日期:2019-02-28 00:00:00
abstract::In this work, a novel technique to create positive-negative tunable liquid crystal lenses is proposed and experimentally demonstrated. This structure is based on two main elements, a transmission line acting as a voltage divider and concentric electrodes that distribute the voltage homogeneously across the active area...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67141-z
更新日期:2020-06-23 00:00:00
abstract::Supercapacitors with aqueous electrolytes and nanostructured composite electrodes are attractive because of their high charging-discharging speed, long cycle life, low environmental impact and wide commercial affordability. However, the energy capacity of aqueous supercapacitors is limited by the electrochemical windo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09854
更新日期:2015-04-21 00:00:00
abstract::The extraction and encoding of acoustical temporal regularities are fundamental for human cognitive auditory abilities such as speech or beat entrainment. Because the comparison of the neural sensitivity to temporal regularities between human and animals is fundamental to relate non-invasive measures of auditory proce...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16774-8
更新日期:2017-11-30 00:00:00
abstract::Quantum key distribution involving decoy-states is a significant application of quantum information. By using three-intensity decoy-states of single-photon-added coherent sources, we propose a practically realizable scheme on quantum key distribution which approaches very closely the ideal asymptotic case of an infini...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15130
更新日期:2015-10-14 00:00:00
abstract::A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. The amplification of 3' ends of cDNA is performed according to the modified classic 3' RACE techniques, therein the more efficient and effective oligo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19420
更新日期:2016-01-13 00:00:00
abstract::Present study aimed to estimate the incidence of recurrent SARS-CoV-2 RNA positivity after recovery from COVID-19 and to determine the factors associated with recurrent positivity. We searched the PubMed, MedRxiv, BioRxiv, the Cochrane Library, ClinicalTrials.gov, and the World Health Organization International Clinic...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-020-77739-y
更新日期:2020-11-26 00:00:00
abstract::Degradation of the biologically potent octapeptide angiotensin Ang II-(1-8) is mediated by the activities of several peptidases. The conversion of Ang II to the septapeptide Ang-(1-7) is of particular interest as the latter also confers organ protection. The conversion is catalyzed by angiotensin-converting enzyme 2 a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45473
更新日期:2017-04-05 00:00:00
abstract::According to the Source Monitoring Framework, the origin of a memory is remembered through the retrieval of specific features (e.g. perceptive, sensitive, affective signals). In two source discrimination tasks, we studied the ability of cuttlefish to remember the modality in which an item had been presented several ho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62335-x
更新日期:2020-03-25 00:00:00
abstract::Animals execute sensorimotor sequences to optimize performance of complex actions series. However, the sensory aspects of these sequences and their dynamic control are often poorly understood. We trained bats to fly to targets at different distances, and analysed their sensory behavior before and during flight to test...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00543-8
更新日期:2017-03-28 00:00:00
abstract::Invasive infections caused by drug-resistant bacteria are frequently responsible for fatal sepsis, morbidity and mortality rates. In this work, we propose a new methodology based on nanosecond high frequency electric field bursts, which enables successful eradication of bacteria in vivo. High frequency (15 kHz) 15-25 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32783-7
更新日期:2018-09-28 00:00:00
abstract::The Smacoviridae has recently been classified as a family of small circular single-stranded DNA viruses. An increasing number of smacovirus genomes have been identified exclusively in faecal matter of various vertebrate species and from insect body parts. However, the genetic diversity and host range of smacoviruses r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41358-z
更新日期:2019-04-08 00:00:00
abstract::Airway smooth muscle (ASM) cells exhibit plastic phenotypic behavior marked by reversible modulation and maturation between contractile and proliferative phenotypic states. Integrins are a class of transmembrane proteins that have been implicated as novel therapeutic targets for asthma treatment. We previously showed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46260-2
更新日期:2019-07-09 00:00:00