Abstract：
:Normalization procedures for the qRT-PCR analysis of miRNA in biological samples are recommended to reduce the variability caused by pre-analytical factors. Since there is no universal standardized normalization strategy for miRNA qRT-PCR studies, we conducted a throughout study to evaluate a panel of small non-coding RNAs (sncRNAs) as reference gene candidate for biomarker studies in serum samples of patients with hepatocellular carcinoma (HCC). Five sncRNAs (miR-1280, miR-1275, SNORD-116, SNORD-68, and U6) were chosen as candidate of reference genes. This study included 122 patients with HCC and was organized into a "pilot phase" consisting of 20 serum samples of HCC patients, and a "validation phase" of 102 patients. Expression level of these candidates were analyzed by qRT-PCR. Assessment of gene stability was performed using four different integrative platforms (geNorm NormFinder, Bestkeeper, and the Delta Ct method). To determine the gene stability during the follow-up of the patient, we extend the analysis of the validation cohort at T1 (1 month after treatment) and T2 (6 month after treatment). MiR-1280 was identified as the most stably expressed reference gene in both pilot and validation phase also during the follow-up. MiR-1280 appears a reliable reference gene candidate in biomarker studies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Pratama MY,Cavalletto L,Tiribelli C,Chemello L,Pascut Ddoi
10.1038/s41598-020-59682-0subject
Has Abstractpub_date
2020-02-21 00:00:00pages
3128issue
1issn
2045-2322pii
10.1038/s41598-020-59682-0journal_volume
10pub_type
杂志文章abstract：:Amorphous silicon oxycarbide (SiOC) is of great technological interest. However, its atomic-level structure is not well understood. Using density functional theory calculations, we show that the clustering tendency of C atoms in SiOC is extremely sensitive to hydrogen (H): without H, the C-C interaction is attractive,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13051
更新日期：2015-08-13 00:00:00
abstract：:Ultrafine-grained die-upset Nd-Fe-B magnets are of importance because they provide a wide researching space to redesign the textured structures. Here is presented a route to obtain a new die-upset magnet with substantially improved magnetic properties. After experiencing the optimized heat treatment, both the coercivi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38335
更新日期：2016-12-06 00:00:00
abstract：:The fungal communities of different soil compartments in mangrove ecosystem are poorly studied. We sequenced the internal transcribed spacer (ITS) regions to characterize the fungal communities in Avicennia marina root-associated soils (rhizosphere and pneumatophore) and bulk soil compartments. The rhizosphere but not...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09281-3
更新日期：2017-08-17 00:00:00
abstract：:Searching, defined for the purpose of the present study as the displacement of an individual to locate resources, is a fundamental behavior of all mobile organisms. In humans this behavior underlies many aspects of everyday life, involving cognitive processes such as sustained attention, memory and inhibition. We expl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19664
更新日期：2016-01-25 00:00:00
abstract：:The intensity of a nonlinear terahertz (THz) source is primarily given by its spectral density. In this letter, we introduce triangular Selinium (Se) as a novel THz emitter and show numerically its superior properties to the currently used crystals for intense THz generation. The excellent phase matching enables the a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08059
更新日期：2015-01-27 00:00:00
abstract：:Charge noise is critical in the performance of gate-controlled quantum dots (QDs). Such information is not yet available for QDs made out of the new material graphene, where both substrate and edge states are known to have important effects. Here we show the 1/f noise for a microscopic graphene QD is substantially lar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08142
更新日期：2015-01-30 00:00:00
abstract：:Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41397
更新日期：2017-02-07 00:00:00
abstract：:In music, the perception of pitch is governed largely by its tonal function given the preceding harmonic structure of the music. While behavioral research has advanced our understanding of the perceptual representation of musical pitch, relatively little is known about its representational structure in the brain. Usin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19222-3
更新日期：2018-01-16 00:00:00
abstract：:A subwoofer generates the lowest frequency range in loudspeaker systems. Subwoofers are used in audio systems for live concerts, movie theatres, home theatres, gaming consoles, cars, etc. During the last decades, numerical simulations have emerged as a cost- and time-efficient complement to traditional experiments in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80092-9
更新日期：2021-01-08 00:00:00
abstract：:Metastatic colorectal cancer is the fourth most common cause of cancer death. Current options in palliation such as hyperthermic intraperitoneal chemotherapy (HIPEC) present severe side effects. Recent research efforts suggested the therapeutic use of oxidant-enriched liquid using cold physical plasma. To investigate ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37169-3
更新日期：2019-01-24 00:00:00
abstract：:Inherited retinal dystrophy (IRD) patients often experience photophobia. However, its mechanism has not been elucidated. This study aimed to investigate the main wavelength of light causing photophobia in IRD and difference among patients with different phenotypes. Forty-seven retinitis pigmentosa (RP) and 22 cone-rod...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71707-2
更新日期：2020-09-09 00:00:00
abstract：:Pericytes regulate vessel stability and pericyte dysfunction contributes to retinopathies, stroke, and cancer. Here we define Notch as a key regulator of pericyte function during angiogenesis. In Notch1(+/-); Notch3(-/-) mice, combined deficiency of Notch1 and Notch3 altered pericyte interaction with the endothelium a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16449
更新日期：2015-11-13 00:00:00
abstract：:Delay embedding-a method for reconstructing dynamical systems by delay coordinates-is widely used to forecast nonlinear time series as a model-free approach. When multivariate time series are observed, several existing frameworks can be applied to yield a single forecast combining multiple forecasts derived from vario...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57255-4
更新日期：2020-01-20 00:00:00
abstract：:Alterations in macronutrient intake can have profound effects on energy intake and whole-body metabolism. For example, reducing protein intake increases energy expenditure, increases insulin sensitivity and decreases body weight in rodents. Fibroblast growth factor 21 (FGF21) signaling in the brain is necessary for th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76593-2
更新日期：2020-11-11 00:00:00
abstract：:Alzheimer's disease (AD) is characterized by excessive production and deposition of amyloid-beta (Aβ) proteins as well as synapse dysfunction and loss. While soluble Aβ oligomers (AβOs) have deleterious effects on synapse function and reduce synapse number, the underlying molecular mechanisms are not well understood. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42548
更新日期：2017-02-13 00:00:00
abstract：:Recently, minocycline, a tetracycline antibiotic, has been reported to improve symptoms of psychiatric disorders and to facilitate sober decision-making in healthy human subjects. Here we show that minocycline also reduces the risk of the 'honey trap' during an economic exchange. Males tend to cooperate with physicall...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01685
更新日期：2013-01-01 00:00:00
abstract：:Mixtures of B4C, α-AlB12 and B powders were reactively spark plasma sintered at 1800 °C. Crystalline and amorphous boron powders were used. Samples were tested for their impact behavior by the Split Hopkinson Pressure Bar method. When the ratio R = B4C/α-AlB12 ≥ 1.3 for a constant B-amount, the major phase in the samp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55888-z
更新日期：2019-12-20 00:00:00
abstract：:Optimal mental workload plays a key role in driving performance. Thus, driver-assisting systems that automatically adapt to a drivers current mental workload via brain-computer interfacing might greatly contribute to traffic safety. To design economic brain computer interfaces that do not compromise driver comfort, it...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80477-w
更新日期：2021-01-12 00:00:00
abstract：:Compromised activity of the DNA repair enzymes may raise the risk of a number of cancers. We analyzed polymorphisms in the Xeroderma Pigmentosum, Complementation Group C (XPC) gene for their correlation with urinary bladder cancer. Ala499Val and Lys939Gln polymorphisms were genotyped in 234 urinary bladder cancer case...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep27018
更新日期：2016-06-01 00:00:00
abstract：:Undulator based synchrotron light sources and Free Electron Lasers (FELs) are valuable modern probes of matter with high temporal and spatial resolution. Laser Plasma Accelerators (LPAs), delivering GeV electron beams in few centimeters, are good candidates for future compact light sources. However the barriers set by...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55209-4
更新日期：2019-12-13 00:00:00
abstract：:It is critical to understand how variations in chemical composition in surface seawater (SSW) affect the chemistry of marine atmospheric aerosols. We investigated the sea-to-air transfer of dissolved organic carbon (DOC) via cruise measurements of both ambient aerosols and SSW in the Oyashio and its coastal regions, t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32864-7
更新日期：2018-10-05 00:00:00
abstract：:Streptomyces coelicolor is a Gram-positive microorganism often used as a model of physiological and morphological differentiation in streptomycetes, prolific producers of secondary metabolites with important biological activities. In the present study, we analysed Streptomyces coelicolor growth and differentiation in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32027-8
更新日期：2018-09-12 00:00:00
abstract：:Apparently mundane, amorphous nanostructures of carbon have optical properties which are as exotic as their crystalline counterparts. In this work we demonstrate a simple and inexpensive mechano-chemical method to prepare bulk quantities of self-passivated, amorphous carbon dots. Like the graphene quantum dots, the wa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28021-9
更新日期：2018-06-27 00:00:00
abstract：:Peatland ecosystem services include drinking water provision, flood mitigation, habitat provision and carbon sequestration. Dissolved organic carbon (DOC) removal is a key treatment process for the supply of potable water downstream from peat-dominated catchments. A transition from peat-forming Sphagnum moss to vascul...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36751
更新日期：2016-11-18 00:00:00
abstract：:Magnetic skyrmions are promising candidates for the next generation of spintronic devices due to their small size and topologically protected structure. One challenge for using these magnetic states in applications lies on controlling the nucleation process and stabilization that usually requires an external force. He...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40705-4
更新日期：2019-03-11 00:00:00
abstract：:Fire blight, caused by the enterobacterium Erwinia amylovora, is a destructive disease, which can affect most members of the Rosaceae family. Since no significant genomic differences have been found by others to explain differences in virulence, we used here a gel-based proteomic approach to elucidate mechanisms and k...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30064-x
更新日期：2018-08-03 00:00:00
abstract：:The human foot and ankle system is equipped with structures that can produce mechanical work through elastic (e.g., Achilles tendon, plantar fascia) or viscoelastic (e.g., heel pad) mechanisms, or by active muscle contractions. Yet, quantifying the work distribution among various subsections of the foot and ankle can ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15218-7
更新日期：2017-11-13 00:00:00
abstract：:The importance of microbial natural products has been widely demonstrated in the search for new antibiotics. However, the functional role of microbial metabolites in nature remains to be deciphered. Several natural products are known to mediate microbial interactions through metabolic exchange. One approach to investi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05532-5
更新日期：2017-07-14 00:00:00
abstract：:Organic-inorganic hybrid perovskite materials have recently been identified as a promising light absorber for solar cells. In the efficient solar cells, the perovskite active layer has generally been fabricated by either vapor deposition or two-step sequential deposition process. Herein, electrochemically deposited Pb...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15889
更新日期：2015-10-29 00:00:00
abstract：:The Salicornia europaea L. (SE) plant is a halophyte that has been widely consumed as a seasoned vegetable, and it has been recently reported to counteract chronic diseases related to oxidative and inflammatory stress. In this study, we performed an initial phytochemical analysis with in vitro biochemical tests and ch...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25381-0
更新日期：2018-05-08 00:00:00
