Fire blight host-pathogen interaction: proteome profiles of Erwinia amylovora infecting apple rootstocks.

Abstract:

:Fire blight, caused by the enterobacterium Erwinia amylovora, is a destructive disease, which can affect most members of the Rosaceae family. Since no significant genomic differences have been found by others to explain differences in virulence, we used here a gel-based proteomic approach to elucidate mechanisms and key players that allow the pathogen to survive, grow and multiply inside its host. Therefore, two strains with proven difference in virulence were grown under controlled conditions in vitro as well as in planta (infected apple rootstocks). Proteomic analysis including 2DE and mass spectrometry revealed that proteins involved in transcription regulation were more abundant in the in planta condition for both strains. In addition, genes involved in RNA processing were upregulated in planta for the highly virulent strain PFB5. Moreover, the upregulation of structural components of the F0F1-ATP synthase are major findings, giving important information on the infection strategy of this devastating pathogen. Overall, this research provides the first proteomic profile of E. amylovora during infection of apple rootstocks and insights into the response of the pathogen in interaction with its host.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Holtappels M,Noben JP,Van Dijck P,Valcke R

doi

10.1038/s41598-018-30064-x

subject

Has Abstract

pub_date

2018-08-03 00:00:00

pages

11689

issue

1

issn

2045-2322

pii

10.1038/s41598-018-30064-x

journal_volume

8

pub_type

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