Induction of the human sperm acrosome reaction by human oocytes.


:The acrosome reaction of human spermatozoa incubated in the presence or absence of vested human oocytes was investigated. All gametes were obtained from human in vitro fertilization (IVF) cases. Spermatozoa were collected after incubation in insemination medium only and following removal of the oocytes from insemination medium during the IVF procedure. After 16 hours of incubation 18.5% of the spermatozoa in insemination medium alone were acrosome-reacted compared to 31.5% for spermatozoa incubated in medium containing oocytes. The acrosome reaction of spermatozoa incubated with fertilized or unfertilized oocytes was also investigated. The percentage of acrosome reaction did not differ (P greater than 0.05) between the two groups (29.7% in the fertilized cases versus 30.7% in the unfertilized cases). Completion of oocyte nuclear maturation did not affect the proportion of acrosome-reacted spermatozoa observed with unfertilized eggs. A similar (P greater than 0.05) percentage of acrosome reacted spermatozoa were observed regardless of whether the unfertilized oocytes had (29%) or had not (35%) reached metaphase II. These findings indicate the acrosome reaction of human spermatozoa is enhanced in the presence of vested human oocytes. Furthermore, there is no apparent correlation between the percentage of the population of spermatozoa that acrosome react in the medium and the potential of an oocyte for fertilization.


Fertil Steril


Fertility and sterility


De Jonge CJ,Rawlins RG,Zaneveld LJ




Has Abstract


1988-12-01 00:00:00














  • Antibiotic prophylaxis for hysteroscopy evaluation of the uterine cavity.

    abstract:OBJECTIVE:To assess the prevalence of infectious complications and the protective effect of prophylactic antibiotic treatment after diagnostic office hysteroscopy in asymptomatic, infertile patients with normal results from transvaginal sonography. DESIGN:Recording of infectious complications after routine hysteroscop...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Kasius JC,Broekmans FJ,Fauser BC,Devroey P,Fatemi HM

    更新日期:2011-02-01 00:00:00

  • Growth factor expression by human oviduct and buffalo rat liver coculture cells.

    abstract:OBJECTIVE:To characterize growth factor gene expression by passaged coculture cell lines demonstrated to enhance in vitro pre-embryo growth. DESIGN:Ribonucleic acids isolated from the isthmus, ampullary, and fimbriae portions of the human oviduct, and from buffalo rat liver cell monolayers were subjected to Northern a...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Barmat LI,Worrilow KC,Paynton BV

    更新日期:1997-04-01 00:00:00

  • The role of the laboratory in the handling of epididymal sperm for assisted reproductive technologies.

    abstract::This report describes the historical experience of our laboratory in the handling and processing of epididymal sperm from men with congenital absence of the vas deferens. Three different methods of sperm preparation were used: swim-up or resuspension in the first group of patients treated, mini-Percoll in the second g...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Ord T,Marello E,Patrizio P,Balmaceda JP,Silber SJ,Asch RH

    更新日期:1992-05-01 00:00:00

  • Sperm recovering efficiency, a mathematical model, is designed to objectively evaluate semen processing techniques and methods.

    abstract:OBJECTIVE:To propose the sperm recovering efficiency (SRE) model, an equation that can be used to objectively quantify the technical skill of individual operators processing semen specimens in an andrology laboratory. Additionally, this equation can be used to compare different semen processing methods and protocols. T...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sanz E,Olivares R

    更新日期:2003-06-01 00:00:00

  • A common Asn680Ser polymorphism in the follicle-stimulating hormone receptor gene is not associated with ovarian response to gonadotropin stimulation in patients undergoing in vitro fertilization.

    abstract:OBJECTIVE:To assess the role of the variant p.Asn680Ser in the follicle-stimulating hormone receptor (FSHR) gene in determining ovarian response in patients undergoing in vitro fertilization (IVF) treatment. DESIGN:Prospective observational study. SETTING:Tertiary referral center for reproductive medicine. PATIENT(S...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Mohiyiddeen L,Newman WG,Cerra C,McBurney H,Mulugeta B,Roberts SA,Nardo LG

    更新日期:2013-01-01 00:00:00

  • Influence of subfertility and assisted reproductive technology treatment on mortality of women after delivery.

    abstract:OBJECTIVE:To compare incidence, risk factors, and etiology of women's deaths in fertile, subfertile, and undergoing assisted reproductive technology (ART) in the years after delivery. DESIGN:Retrospective cohort. SETTING:University hospital. PATIENT(S):Women who had delivered in Massachusetts. INTERVENTION(S):This ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Coddington CC,Gopal D,Cui X,Cabral H,Diop H,Stern JE

    更新日期:2020-03-01 00:00:00

  • Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis.

    abstract:OBJECTIVE:To examine the impact of a recent surgery on development of endometriosis-related adhesions in a chimeric model and to determine the therapeutic efficacy of pioglitazone (PIO). DESIGN:Human endometrial biopsies were maintained in E(2) with or without PIO for 24 h before intraperitoneal injection into immunoc...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Herington JL,Crispens MA,Carvalho-Macedo AC,Camargos AF,Lebovic DI,Bruner-Tran KL,Osteen KG

    更新日期:2011-03-15 00:00:00

  • Treatment of uterine leiomyomata with a luteinizing hormone-releasing hormone agonist: the possibility of nonsurgical management in selected perimenopausal women.

    abstract:OBJECTIVE:To evaluate the efficacy of luteinizing hormone-releasing hormone agonist (LH-RH-a) in the treatment of leiomyomata. DESIGN:A retrospective randomized trial. SETTING:Hospital department of obstetrics and gynecology. PATIENTS:Twenty-five women, ages 36 to 54 years with symptomatic uterine leiomyomata, were ...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Nakamura Y,Yoshimura Y,Yamada H,Ubukata Y,Ando M,Suzuki M

    更新日期:1991-05-01 00:00:00

  • Assessment of FSHR variants and antimüllerian hormone in infertility patients with a reduced ovarian response to gonadotropin stimulation.

    abstract:OBJECTIVE:To study women with a poor response to ovarian hormone stimulation, known as low responders. Genetic defects in the FSH receptor gene (FSHR) were analyzed as well as antimüllerian hormone (AMH) for ovarian reserve. DESIGN:Retrospective cohort study. SETTING:University hospital. PATIENT(S):Two hundred fifty...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Binder H,Strick R,Zaherdoust O,Dittrich R,Hamori M,Beckmann MW,Oppelt PG

    更新日期:2012-05-01 00:00:00

  • Uterine artery occlusion at myomectomy.

    abstract:OBJECTIVE:To demonstrate three approaches to uterine artery occlusion at time of myomectomy as a blood-sparing intraoperative technique. DESIGN:A step-by-step explanation of the procedure with surgical video footage. SETTING:Academic medical center. PATIENT(S):Patients undergoing laparoscopic myomectomy, for whom a ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Zakhari A,Sanders AP,Murji A

    更新日期:2019-05-01 00:00:00

  • Advanced surgical sperm recovery is a viable option for intracytoplasmic sperm injection in patients with obstructive or nonobstructive azoospermia.

    abstract:OBJECTIVE:To determine whether advanced sperm retrieval is appropriate in cases of obstructive and nonobstructive azoospermia. DESIGN:Prospective controlled study. SETTING:Tertiary care center. PATIENT(S):Men with obstructive and nonobstructive azoospermia, and their partners. INTERVENTION(S):Surgical sperm retriev...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Jaroudi K,Coskun S,Hollanders J,Al-Hassan S,Al-Sufayan H,Atared A,Merdad T

    更新日期:1999-09-01 00:00:00

  • Triplet tubal pregnancy treated by outpatient laparoscopic salpingostomy.

    abstract::To our knowledge, this represents the first case of a laparoscopically treated triplet EP and the first time that a double EP in the same tube was treated conservatively (with preservation of the tube). Multiple EPs may be more common than currently thought, and our report offers an alternative explanation for at leas...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Frishman GN,Steinhoff MM,Luciano AA

    更新日期:1990-11-01 00:00:00

  • Buoyant density and sedimentation dynamics of HIV-1 in two density-gradient media for semen processing.

    abstract:OBJECTIVE:To compare buoyant density and sedimentation kinetics of human immunodeficiency virus 1 (HIV-1) in two sperm-washing media, Percoll and Pureception. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):None. INTERVENTION(S):Buoyant density and sedimentation kinetics of HIV-1 particles (MOLT-4/...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kuji N,Yoshii T,Hamatani T,Hanabusa H,Yoshimura Y,Kato S

    更新日期:2008-11-01 00:00:00

  • Impairment of the developmental potential of frozen-thawed human zygotes obtained after intracytoplasmic sperm injection.

    abstract:OBJECTIVE:To evaluate the effects of cryopreservation on the survival, cleavage, and morphology of embryos and on the implantation and embryonic loss rates of human zygotes obtained after ICSI compared with frozen-thawed zygotes obtained after traditional IVF. A further objective was to evaluate the same parameters in ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Macas E,Imthurn B,Borsos M,Rosselli M,Maurer-Major E,Keller PJ

    更新日期:1998-04-01 00:00:00

  • Specific antibodies and immunoglobulins in the oviductal fluid of the rhesus monkey.

    abstract::Levels of specific antibodies against model antigens, immunoglobulins G and A and also albumin, in oviductal fluid were studied in the rhesus monkey during the periovulatory period. Animals were systemically or intravaginally immunized against T4 coliphages. Attempts to induce ovulation were made with human menopausal...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Yang SL,Schumacher GF,Broer KA,Holt JA

    更新日期:1983-03-01 00:00:00

  • The nitrofurans as sperm-immobilizing agents, their tissue toxicity, and their clinical application in vasectomy.

    abstract::Nitrofurantoin sodium and nitorfurazone, in certain critical concentrations, have the ability to immobilize spermatozoa. In this study, this effect was consisitent with both 5:1, and 10:1 dilutions. Histologic examination of the effects of these drugs as intraoperative vas irrigants revealed no apparent tissue toxicit...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Albert PS,Salerno RG,Kapoor SN,Davis JE

    更新日期:1975-06-01 00:00:00

  • Transvaginal ultrasonography combined with water-contrast in the rectum in the diagnosis of rectovaginal endometriosis infiltrating the bowel.

    abstract::This prospective study evaluated the efficacy of transvaginal ultrasonography combined with water-contrast in the rectum (RWC-TVS) in the diagnosis of rectal infiltration in 35 women with rectovaginal endometriosis; ultrasonographic findings were compared with surgery and histology. The sensitivity of RWC-TVS in ident...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Menada MV,Remorgida V,Abbamonte LH,Fulcheri E,Ragni N,Ferrero S

    更新日期:2008-03-01 00:00:00

  • Leyomiomatosis peritonealis disseminata associated with ovarian endometriosis in a patient submitted to hysteroscopic myomectomy.

    abstract:OBJECTIVE:To describe laparoscopic management of a case of leyomiomatosis peritonealis disseminata associated with ovarian endometriosis. DESIGN:Surgical video article. SETTING:Academic hospital. PATIENT:We present a case of a 30-year-old woman referred to our clinic for abdominal and pelvic pain and dyspareunia. A ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Mabrouk M,Arena A,Raimondo D,Parisotto M,Caprara G,Seracchioli R

    更新日期:2019-06-01 00:00:00

  • The single or dual administration of the gonadotropin-releasing hormone antagonist Cetrorelix in an in vitro fertilization-embryo transfer program.

    abstract:OBJECTIVE:To assess the ability of a GnRH antagonist (Cetrorelix, Asta Medica AG, Frankfurt, Germany) to prevent premature LH surges in an IVF-ET program using a simple protocol with one or two administrations. DESIGN:Controlled ovarian hyperstimulation was carried out in 17 women with three ampules a day of hMG, star...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Olivennes F,Fanchin R,Bouchard P,de Ziegler D,Taieb J,Selva J,Frydman R

    更新日期:1994-09-01 00:00:00

  • Potential chemoprotective effect of melatonin in cyclophosphamide- and cisplatin-induced testicular damage in rats.

    abstract:OBJECTIVE:To evaluate the effect of melatonin on cyclophosphamide (CP)- and cisplatin-induced testicular toxicity with use of sperm parameters and biochemical and histopathologic approaches. DESIGN:Experimental study. SETTING:Vakif Gureba Hospital, Istanbul, Turkey. ANIMALS:Six-week-old adult male Wistar albino rats...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Ilbey YO,Ozbek E,Simsek A,Otunctemur A,Cekmen M,Somay A

    更新日期:2009-09-01 00:00:00

  • Interleukin-8 gene and protein expression are up-regulated by interleukin-1beta in normal human ovarian cells and a granulosa tumor cell line.

    abstract:OBJECTIVE:To evaluate the expression, regulation, and role of interleukin (IL)-8 in human ovary. DESIGN:Prospective study. SETTING:University hospital. PATIENT(S):Sixteen premenopausal women. INTERVENTION:Follicular fluid and granulosa lutein cells (GLCs) were collected during IVF cycles. Ovarian stromal and theca ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Fujii A,Harada T,Yamauchi N,Iwabe T,Nishi Y,Yanase T,Nawata H,Terakawa N

    更新日期:2003-01-01 00:00:00

  • Reconstruction of the ovary containing large endometriomas by an extraovarian endosurgical technique.

    abstract:OBJECTIVE:To describe and evaluate the surgical treatment of large ovarian endometriomas by an extraovarian endosurgical technique. DESIGN:Prospective case series study. SETTING:University center and tertiary referral center for endoscopic surgery. PATIENTS:Patients with large ovarian endometriomas. INTERVENTION:A ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Brosens IA,Van Ballaer P,Puttemans P,Deprest J

    更新日期:1996-10-01 00:00:00

  • Testicular reserve of testosterone precursors in primary testicular failure.

    abstract::To explore the abnormal steroidogenesis in subjects with primary testicular failure, we measured serum levels of testosterone (T) and its precursors androstenedione (delta 4A) and 17-hydroxyprogesterone (17-OHP) in the basal state and after stimulation by human chorionic gonadotropin (hCG) for 4 hours (acute reserve) ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Glass AR,Vigersky RA

    更新日期:1982-07-01 00:00:00

  • BRCA mutation carriers show normal ovarian response in in vitro fertilization cycles.

    abstract:OBJECTIVE:To evaluate the association between carriage of BRCA1/2 mutations and ovarian performance, as demonstrated by in vitro fertilization (IVF) outcomes. DESIGN:Retrospective cohort study. SETTING:Two tertiary IVF centers. PATIENT(S):BRCA mutation carriers undergoing IVF for preimplantation genetic diagnosis (P...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究


    authors: Shapira M,Raanani H,Feldman B,Srebnik N,Dereck-Haim S,Manela D,Brenghausen M,Geva-Lerner L,Friedman E,Levi-Lahad E,Goldberg D,Perri T,Eldar-Geva T,Meirow D

    更新日期:2015-11-01 00:00:00

  • Reproductive performance of women after two tubal ectopic pregnancies.

    abstract::Twenty-four women who had undergone total salpingectomy due to ectopic pregnancy and who subsequently underwent a laparotomy for a second ectopic pregnancy in their opposite tube were treated by the author. Treatment consisted of linear salpingostomy (n = 20) and partial salpingectomy (n = 4). The intrauterine pregnan...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Tulandi T

    更新日期:1988-07-01 00:00:00

  • Thrombin activatable fibrinolysis inhibitor and clot lysis time in women with recurrent miscarriage associated with the antiphospholipid syndrome.

    abstract::Antiphospholipid syndrome patients with recurrent miscarriage have an impairment in fibrinolysis demonstrated by prolonged clot lysis time (CLT) that cannot be attributed to differences in thrombin activatable fibrinolysis inhibitor (TAFI) antigen levels. Patients with unexplained recurrent miscarriage have an impairm...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Martínez-Zamora MA,Creus M,Tassies D,Bové A,Reverter JC,Carmona F,Balasch J

    更新日期:2010-11-01 00:00:00

  • Cytomegalovirus (CMV) infection--related to male and/or female infertility factors?

    abstract:OBJECTIVE:To screen for cytomegalovirus (CMV) infection in male and female partners of subfertile couples and to analyze a potential relationship with clinically relevant parameters of male and female factor fertility. DESIGN:Prospective study. SETTING:Outpatient infertility clinic of a university-based hospital. PA...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Eggert-Kruse W,Reuland M,Johannsen W,Strowitzki T,Schlehofer JR

    更新日期:2009-01-01 00:00:00

  • Treatment of cervical mucus with lectins: effect on sperm migration.

    abstract::Treatment of midcycle human cervical mucus with the lectins Ulex europaeus agglutinin I (UE), wheat germ agglutinin (WGA), and peanut agglutinin (PA) did not alter the ability of spermatozoa to enter and migrate through the mucus. These lectins form glycoconjugates by binding to L-fucose (UE), N-acetylglucosamine and ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Snyder MG,Zaneveld LJ

    更新日期:1985-11-01 00:00:00

  • Assisted fertility using electroejaculation in men with spinal cord injury--a review of literature.

    abstract:OBJECTIVE:To review the effectiveness of rectal probe electroejaculation in terms of pregnancy in treatment of anejaculatory males due to spinal cord injury. The characteristics of electroejaculates also are examined. DATA IDENTIFICATION:All English language studies reporting pregnancies as a result of electroejaculat...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Chung PH,Yeko TR,Mayer JC,Sanford EJ,Maroulis GB

    更新日期:1995-07-01 00:00:00

  • Peritoneal ectopic lesions from women with endometriosis show abnormalities in progesterone-dependent glycan expression.

    abstract::Examination of 12 paired peritoneal ectopic and eutopic endometria for histochemical binding of Dolichos biflorus agglutinin, normally found in the mid-late secretory part of the cycle, showed a failure of lectin binding in 9 of 11 secretory-phase lesions although the eutopic specimens generally stained normally. This...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Jones CJ,Nardo LG,Litta P,Fazleabas AT

    更新日期:2009-04-01 00:00:00