Abstract:
:Reductive stress, which is due to a paradoxical excess of antioxidants such as reduced glutathione (GSH) and GSH-related enzymes associated with decreased oxidant levels, has emerged as a pathogenetic mechanism of myocardial damage in pathological conditions such as protein aggregation cardiomyopathy. Notably, in the aged heart a cardiomyopathy-like pathology occurs leading to myocardial dysfunction. Whether reductive stress, or instead its counterpart oxidative stress, is operative in the aged mammalian heart needs to be elucidated also for the different therapeutic implications of such redox stress conditions. In the present investigation, we assessed GSH and the specific enzymatic activities of γ-glutamylcysteine synthetase (γ-GCS), glutathione reductase (GSSG-Red) and selenium-dependent glutathione peroxidase (GSH-Px) as endogenous antioxidants, together with oxidized glutathione (GSSG) and the glutathione redox ratio (GSH/GSSG), in the aerobically perfused hearts of aged rabbits (about 4.5 years old) and young adult control rabbits (3-4 months old). We also assessed in the aged and control hearts H2O2 and catalytically active low molecular weight iron (LMWI) as oxidant forces, as well as fluorescent damage products of lipid peroxidation (FDPL) and protein carbonyls (PC) as biomarkers of lipid and protein oxidation. Moreover, the effects of 4.5 mM N-acetylcysteine (NAC) as reducing thiol antioxidant were studied on hemodynamic parameters and lipid peroxidation in the perfused hearts of the aged and control rabbits. The levels of GSH and of the GSH/GSSG ratio were lower, and those of GSSG higher, in the aged than in the control hearts. The aged hearts were also characterized by decreased activities of the antioxidant enzymes γ-GCS, GSSG-Red and GSH-Px, as well as by heightened levels of H2O2, LMWI, FDPL and PC, highlighting the occurrence of aging-dependent oxidative stress. Associated with such biochemical alterations, hemodynamic dysfunction occurred in the aged rabbit hearts, as evidenced by lowered developed pressure (DP) and enhanced end-diastolic pressure (EDP) with decreased coronary flow (CF). Remarkably, NAC administration significantly improved DP and EDP, and lowered lipid peroxidation, electively in the aged hearts. In conclusion, oxidative and not reductive stress is operative in the aged rabbit heart, whose hemodynamic dysfunction is improved by NAC together with reduction in myocardial lipid peroxidation.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Lapenna D,Ciofani G,Lelli Chiesa P,Porreca Edoi
10.1016/j.exger.2020.110871subject
Has Abstractpub_date
2020-02-06 00:00:00pages
110871eissn
0531-5565issn
1873-6815pii
S0531-5565(19)30738-7journal_volume
134pub_type
杂志文章abstract:BACKGROUND:Physical activity and sedentary behavior are modifiable risk factors for non-communicable disease and healthy ageing, however the majority of older adults remain insufficiently active. Digital behavior change interventions (DBCI) have the potential to reach many older adults to promote physical activity and ...
journal_title:Experimental gerontology
pub_type: 杂志文章,meta分析
doi:10.1016/j.exger.2019.02.020
更新日期:2019-06-01 00:00:00
abstract::Fatigue is a common complaint in older people. Laboratory-induced muscle fatigue has been found to affect physical functions in older populations but these protocols are rigorous and are unlikely to accurately reflect daily activities. This study used an ecological approach to determine the effects of a busy day on se...
journal_title:Experimental gerontology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.exger.2018.03.009
更新日期:2018-06-01 00:00:00
abstract::Neural correlates of placebo analgesia (PA) in patients with neurocognitive disorders have not yet been elucidated. The present study aimed to evaluate how and to what extent executive (dys)functions of the medial prefrontal cortex (MPFC) may be related to PA. To this end, twenty-three subjects complaining of differen...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2019.01.011
更新日期:2019-04-01 00:00:00
abstract::Accumulation of altered proteins is the most common molecular symptom of ageing. Altered proteins are also associated with many age-related pathologies. Altered proteins are continuously produced but are normally selectively degraded by cellular proteases; their accumulation during ageing may be explained by either or...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2006.03.004
更新日期:2006-05-01 00:00:00
abstract::Histopathological and autofluorescence investigations were carried out to study lipofuscin pigment accumulation in various age groups of mice. Qualitative studies revealed an increase of lipofuscin pigment accumulation in old animals. Quantitative studies showed a significant increase in the percentage of pigmented ne...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(82)90041-9
更新日期:1982-01-01 00:00:00
abstract::Given the increased concerns about the degenerative decline in the physical performance of the elderly, there is a need for developing effective strategies to suppress the age-related loss of skeletal muscle mass and functional capacity through a lifestyle intervention. This randomized controlled trial examined whethe...
journal_title:Experimental gerontology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.exger.2016.11.003
更新日期:2017-01-01 00:00:00
abstract::Mitochondrial proton leak may modulate reactive oxygen species (ROS) production and play a role in aging. The purpose of this study was to determine proton leak across the life span in skeletal mitochondria from calorie-restricted and UCP2/3 overexpressing mice. Proton leak in isolated mitochondria and markers of oxid...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2008.09.010
更新日期:2008-12-01 00:00:00
abstract::Apoptosis plays an important role in atrophy and sarcopenia in skeletal muscle. Recent evidence suggests that insufficient heat shock proteins (HSPs) may contribute to apoptosis and muscle wasting. In addition, long-term caloric restriction (CR) and lifelong wheel running exercise (WR) with CR provide significant prot...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2015.06.007
更新日期:2015-09-01 00:00:00
abstract::In order to establish the role of calcitonin (CT) in postmenopausal bone loss, we studied CT metabolism in 25 pre- and postmenopausal women. Postmenopausal women presented a highly significant reduction of CT basal levels compared to premenopausal females (p less than 0.01). Also, production rates of CT in osteoporoti...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(90)90072-a
更新日期:1990-01-01 00:00:00
abstract::Calorie restriction (CR) may affect glucose tolerance via modulation of the insulin action in skeletal muscle. The present study investigated the effect of CR initiated at middle age in rats bearing glucose intolerance, in comparison with CR at a younger age. Male F344 rats at 2.5 and 18months (mo) of age were fed ad ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2006.06.055
更新日期:2006-09-01 00:00:00
abstract::The interrelationship between muscle strength, motor unit (MU) number, and age is poorly understood, and in this study we sought to determine whether age-related differences in muscle strength are moderated by estimates of functioning MU number and size. Eighteen older adults (OA; 67 ± 1.20 years) and 24 young adults ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2013.06.008
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVES:There is a scarcity of data on the association between mild cognitive impairment (MCI) and falls, especially from low- and middle-income countries (LMICs) where 70% of all older adults reside. Thus, we investigated the association between MCI and fall-related injury among older adults residing in six LMICs (...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2020.111222
更新日期:2020-12-29 00:00:00
abstract::As a major intracellular source of reactive oxygen species (ROS), mitochondria are involved in aging and lifespan regulation. Using the yeast chronological aging model, researchers have identified conserved signaling pathways that affect lifespan by modulating mitochondrial functions. Caloric restriction and a genetic...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2011.08.007
更新日期:2011-11-01 00:00:00
abstract::Ethanol ingestion has been implicated in accidental hypothermia of the elderly, but there is no prior data on the relative sensitivity to alcohol of aged homeotherms that might account for disproportionate dysthermia in this age group. To assess their sensitivity, ethanol (0.5-2.0 g/kg) or H20 was given by gavage to s...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(83)90047-5
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND/AIM:Age-related metabolic diseases are often associated with low-grade inflammation. The aim of the present study was to investigate the role of the transcriptional co-activator PGC-1α in the potential beneficial effects of exercise training and/or resveratrol in the prevention of age-associated low-grade in...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2013.07.015
更新日期:2013-11-01 00:00:00
abstract::Age-related changes in cancer mortality risk are important for understanding the processes of disease and aging interaction. The extent to which these age changes differ by sex further contributes to this understanding but has not been well studied to date. We conducted a systematic examination of dynamics and heterog...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2012.06.009
更新日期:2012-09-01 00:00:00
abstract::Increased lifespan can be associated with greater resistance to many different stressors, most notably thermal stress. Such hormetic effects have also been found in C. elegans where short-term exposure to heat lengthens the lifespan. Genetic investigations have been carried out using mutation perturbations in a single...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2012.05.005
更新日期:2012-08-01 00:00:00
abstract::Previous studies have shown that adult mice increase cold-induced heat production as a result of repeated exposures to cold, but that aged mice do not. The objective of the present study was to investigate changes in heat loss during repeated cold exposures in adult and aged C57BL/6J mice. Mice were partially restrain...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(96)00131-3
更新日期:1997-05-01 00:00:00
abstract::Non-insulin dependent diabetes mellitus (NIDDM) has been associated with a number of physiological consequences including neuropathy, retinopathy and incidence of vascular disease. Recently, several authors reviewed studies that suggested that NIDDM is associated with cognitive impairments leading to a higher incidenc...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/s0531-5565(03)00153-0
更新日期:2003-09-01 00:00:00
abstract::The mechanisms responsible for age-associated hearing loss are still incompletely characterized. In this study, we used a murine model of age-dependent hearing loss and evaluated whether this condition is associated with vascular modifications of the structures of the inner ear. We used old C57BL/6J mice that are affe...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2004.06.003
更新日期:2004-08-01 00:00:00
abstract::Aging has a profound effect on the immune system, and both innate and adaptive arms of the immune system show functional decline with age. In response to infection with intracellular microorganisms, old animals mobilize decreased numbers of antigen-specific CD8+ T cells with reduced production of effector molecules an...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2018.01.011
更新日期:2018-05-01 00:00:00
abstract::Concanavalin-A (Con-A) reactivity was studied to identify the tissue-specificity of cells established from various normal human tissues. Cells were treated with Con-A-labelled human red blood cells (C-RBC). C-RBC was not absorbed on the cells derived from the bone marrow, skin and liver. Lung-derived fibroblast cells ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(83)90040-2
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVES:To examine the individual association between BMI and level of serum uric acid (SUA) among the very elderly Chinese population. METHODS:A survey was conducted on 870 long-lived subjects (aged ≥90years). Subjects were divided into four groups according to quartile of BMI (<16.6, 16.6-18.9, 18.9-21.1, ≥21.1kg...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2012.05.008
更新日期:2012-08-01 00:00:00
abstract::Understanding telomere biology is of utmost importance for aging and cancer research. An essential tool is the determination of telomere length, which traditionally is done by telomere restriction fragment analysis, a laborious and time consuming method. Therefore, large efforts have been made to establish alternative...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2005.09.013
更新日期:2006-02-01 00:00:00
abstract::The age-dependent modifications of both basal and lymphokine-induced natural killer (NK) activity were analyzed in Balb/c inbred mice by measuring either the percentage of specific lytic activity in a 51 Cr release assay or the percentage of cells capable of binding the YAC-1 target cells. The basal lytic activity of ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(89)90014-4
更新日期:1989-01-01 00:00:00
abstract::Changes associated with the age-related decline of physiological functions, and their relation with mortality rates, are thoroughly being investigated in the aging research field. We used the Mediterranean fruit fly Ceratitis capitata, largely studied by biodemographers, as a model for functional senescence studies. T...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2012.04.001
更新日期:2012-06-01 00:00:00
abstract::A mathematical model of cellular metabolism is used to relate the rates of cell division and waste production to the concentrations of oxygen and glucose in the medium in which a normal diploid cell culture is grown. The metabolic model in tandem with an earlier waste-content model based on the waste-product theory of...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(91)90073-u
更新日期:1991-01-01 00:00:00
abstract::Pioglitazone (Piog) activates peroxisome proliferator activated receptor-γ (PPARγ) and is widely used in clinic for the treatment of diabetes mellitus. PPARγ in various tissues has the essential regulatory role of multiple metabolic function, suggest that PPARγ signaling may contribute to aging processes. However, lit...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.12.002
更新日期:2018-02-01 00:00:00
abstract::A summary of the Eleventh International Symposium on the Neurobiology and Neuroendocrinology of Aging that was held in July 29-August 3 in Bregenz, Austria, is presented. Sixteen of the speakers who presented at the conference submitted review papers covering the topic of their presentation as well as an overview of t...
journal_title:Experimental gerontology
pub_type: 社论
doi:10.1016/j.exger.2013.01.003
更新日期:2013-07-01 00:00:00
abstract::A growing body of evidence indicates that dysregulation of cerebral biometals (Fe, Cu, Zn) and their interactions with APP and Abeta amyloid may contribute to the Alzheimer's amyloid pathology, and thus metal chelation could be a rational therapeutic approach for interdicting AD pathogenesis. However, poor target spec...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2004.08.016
更新日期:2004-11-01 00:00:00