Abstract:
BACKGROUND:The pathogenesis of myasthenia gravis (MG) depends upon T and B cells, as well as complement and cytokines. The activation of functional subpopulations of T and B cells is the basis of the immune response. However, the activation levels of T and B cell subsets remain unclear in the pathogenesis of MG. Here, we evaluated the proportions of T and B cell subsets and related cytokines in ocular MG (oMG) patients and generalized MG (gMG) patients, and analyzed the potential roles of T and B cell subsets in the pathogenesis of oMG. METHODS:In total, 16 patients with oMG, 31 patients with gMG, and 20 healthy controls (HCs) were included in this study. Peripheral blood mononuclear cells (PBMCs) were separated from venous blood via density centrifugation. The percentages of CD3+CD4+ T cells, CD3+CD8+ T cells, CD4+CD25+CD127- regulatory T cells (Tregs), CD19+CD27+CD38- memory B cells, CD19+CD24hiCD27+ regulatory B cells (Bregs), CD19+CD38+CD138+ plasma cells, and CD19+CD40+ B cells in PBMCs were detected by flow cytometry. The levels of interleukin (IL)-2, IL-4, IL-6, IL-10, IL-17, and interferon (IFN)-γ in serum were detected by enzyme linked immunosorbent assay (ELISA). Differences in T and B cell subsets and related cytokines were compared among the three groups of participants. RESULTS:The proportions of CD19+CD27+CD38- memory B cells were significantly higher in the oMG and gMG groups than in the HC group (P = 0.004; P < 0.001), whereas the proportion of CD19+CD27+CD38- memory B cells was significantly lower in the oMG group than in the gMG group (P < 0.001). Moreover, the proportion of CD19+CD24hiCD27+ Bregs was significantly higher in the oMG group than in the gMG and HC groups (P = 0.001). The proportion of CD4+CD25+CD127- Tregs was significantly lower in the gMG group than in the oMG and HC groups (P < 0.001). Finally, the level of serum IL-10 was significantly higher in the oMG group than in the gMG and HC groups (P < 0.05). Compared with the HC group, serum IL-2 levels were significantly increased in the oMG and gMG groups (P = 0.016; P = 0.002). DISCUSSION:The reduced ratios of Tregs and Bregs may contribute to the progression of oMG to gMG, and the increased proportion of memory B cells may be closely related to the progression of oMG. IL-2 and IL-10 are important in the development of oMG.
journal_name
Int Immunopharmacoljournal_title
International immunopharmacologyauthors
Hu Y,Wang J,Rao J,Xu X,Cheng Y,Yan L,Wu Y,Wu N,Wu Xdoi
10.1016/j.intimp.2019.106130subject
Has Abstractpub_date
2020-03-01 00:00:00pages
106130eissn
1567-5769issn
1878-1705pii
S1567-5769(19)31378-5journal_volume
80pub_type
杂志文章abstract::New strategies that stimulate cell-mediated immunity (CMI) against tumors and inhibit regulatory T cells are needed to improve the outcome of cancer immunotherapy. The aim of this study was to enhance the anti-tumor immunity of gp96 vaccine through naloxone administration. Therefore, we used BALB/c mouse model of fibr...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2009.08.008
更新日期:2009-11-01 00:00:00
abstract::Cutaneous toxicities are the commonest side effects in patients with cancer treated using epidermal growth factor receptor inhibitors such as erlotinib. For patients with such toxicities, there is a lack of safe, effective pharmacological agents. Here we established a skin toxicity model and investigated the preventiv...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.01.023
更新日期:2019-04-01 00:00:00
abstract::Deciphering the molecular mechanisms of amyloid pathology and glial cell-mediated neuroinflammation, offers a novel avenue for therapeutic intervention against neurodegeneration. Recent findings demonstrate a crucial link between activation of glycogen synthase kinase-3β (GSK-3β), amyloid deposition and a neuroinflamm...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.02.053
更新日期:2019-05-01 00:00:00
abstract::Paraquat is one of the most extensively used herbicides and has high toxicity for humans and animals. However, there is no effective treatment for paraquat poisoning. The aim of the present study was to evaluate the effects of chloroquine on paraquat-induced lung injury in mice. Mice received a single intraperitoneal ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2017.02.020
更新日期:2017-05-01 00:00:00
abstract::The presence of thrombin-cleaved form of osteopontin well correlated with various inflammatory disease activities in not only rodents, but also humans. We previously demonstrated that the blocking of the interaction of a cryptic epitope within osteopontin, which is exposed by thrombin cleavage, with its integrins by s...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2007.06.009
更新日期:2007-11-01 00:00:00
abstract::The purpose of this review is to collate current knowledge and recent advances in molecular mechanism behind the immediate type hypersensitivity of foods. Food allergy is a growing concern of human health in developed as well as developing countries now days. Food allergic reactions are mostly IgE mediated and also kn...
journal_title:International immunopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.intimp.2012.05.018
更新日期:2012-08-01 00:00:00
abstract:AIM:Due to the significant increase in the antimicrobial resistance of Acinetobacter baumannii (A. baumannii), new drugs to block the progression of infection are strongly needed. Epigallocatechin-3-gallate (EGCG), a major component of green tea, has exhibited potential activity against A. baumannii in vitro. The aim o...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.107346
更新日期:2021-01-04 00:00:00
abstract::Visfatin plays an important role in inflammatory and metabolic conditions. In this study, the effects of septic stress on the serum, white-adipose-tissue (WAT), and liver visfatin levels of male and female rats were examined. Both gonadally intact (sham) and ovariectomized (OVX) female rats were used in order to evalu...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2016.04.002
更新日期:2016-06-01 00:00:00
abstract::Interleukin (IL)-35 plays an important role in the pathogenesis of rheumatoid arthritis (RA), which is characterized by tumor necrosis factor (TNF)-α activated bone loss beginning early and persisting over time. The aim of this study was to explore the effects and signaling pathway of IL-35 on osteoblasts differentiat...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105810
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Spontaneous abortion is a common disease in human pregnancy. Increasing evidence suggests that proper function of trophoblasts and immune balance of the maternal-fetal interface are crucial for successful pregnancy. Macrophages are involved in the maternal-fetal immune microenvironment. However, mechanisms a...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106981
更新日期:2020-11-01 00:00:00
abstract::Clematichinenoside AR (AR), a major active ingredient extracted from traditional Chinese herb Clematis chinensis Osbeck, has been demonstrated to possess anti-inflammatory and immune-modulatory activities in the treatment of experimental rheumatoid arthritis (RA). The therapeutic potential of AR was supposed to be clo...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.04.010
更新日期:2019-10-01 00:00:00
abstract::While imiquimod (IMQ) has been widely used in dermatology, its side effect manifested as dermatitis couldn't be ignored. However, the underlying mechanism has not been fully understood. Considering the clinical features of IMQ-related dermatitis similar to pseudo-allergic reaction and the presence of large numbers of ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106258
更新日期:2020-04-01 00:00:00
abstract::Isodeoxyelephantopin (IDET) has been identified as an anti-tumor natural constituent whose anti-tumor activity and mechanism have been widely investigated. Since the occurrence and development of cancer usually accompany with inflammation, and tumor signaling shares many components with inflammation signaling, the age...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106528
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:This meta-analysis was performed to evaluate the efficacy of ulinastatin (UTI) and thymosin α1 (Tα1) based immunomodulatory strategy in sepsis patients. METHODS:A systematic search was made of MEDLINE, Cochrane, ISI Web of Science and SCOPUS databases. Randomized clinical trials on treatment of sepsis with t...
journal_title:International immunopharmacology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.intimp.2015.10.026
更新日期:2015-12-01 00:00:00
abstract:UNLABELLED:A prospective study was performed to evaluate low dose Anti-Thymocyte Globulin (ATG) and Rituximab along with high dose Sirolimus as induction agents for reducing the incidence of acute rejection in renal transplantation. 66 patients who were to undergo live renal transplantation were divided into the low ri...
journal_title:International immunopharmacology
pub_type: 临床试验,杂志文章
doi:10.1016/j.intimp.2006.09.013
更新日期:2006-12-20 00:00:00
abstract::Recombinant immunotoxins are chimeric proteins composed of a targeting peptide that binds to a specific tumor antigen and a toxin protein killing target cells. Recombinant immunotoxin exhibits potent cancer inhibiting effects both in vivo and in vitro. However, the non-specific toxicity causes severe syndromes limitin...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.11.039
更新日期:2019-01-01 00:00:00
abstract::Compared with freshly isolated peripheral blood natural killer (NK) cells, the YT and NK-92 cell lines are characterized by elevated cytolytic activity. The molecular mechanisms underlying the rapid proliferation and enhanced lytic activity of NK cell lines are poorly understood. Investigation of these cell lines reve...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2004.12.016
更新日期:2005-05-01 00:00:00
abstract::Inflammation plays an important role in the pathogenesis of cerebral ischemia. Syringin (SYR) is an active substance isolated from Acanthopanax senticosus plants, and possesses anti-inflammatory and neuroprotective properties. However, its effects on cerebral ischemic injury, as well as the underlying molecular events...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.107268
更新日期:2021-01-01 00:00:00
abstract::The current study focused on the pharmacodynamic activity components of Gentianopsis paludosa against ulcerative colitis (UC) fibrosis including symptoms of intestinal diarrhea and inflammatory. Trinitro-benzene-sulfonic acid induced UC model rats were gavaged with gradient polarity extracts respectively from ethanol-...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2017.01.001
更新日期:2017-05-01 00:00:00
abstract::Anti-citrullinated protein antibodies (ACPAs) are a key serological marker of rheumatoid arthritis (RA). To investigate whether miR-let7a downregulated ACPA-induced macrophage activation and arthritis, miR-let7a levels were assessed in the synovial fluid from patients with RA or osteoarthritis (OA). In addition, expre...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2017.08.001
更新日期:2017-10-01 00:00:00
abstract::Recent studies on the immunosuppressive effects of ultraviolet radiation (UVR) and the related resistance to infections in rodents and humans are presented. The waveband dependency of trans-to-cis isomerisation of urocanic acid in the stratum corneum and the role of DNA damage in UVR-induced erythema and immunosuppres...
journal_title:International immunopharmacology
pub_type: 杂志文章,评审
doi:10.1016/s1567-5769(01)00178-3
更新日期:2002-02-01 00:00:00
abstract::Glabridin has multiple pharmacological activities including anti-microbial, anti-atherosclerotic, anti-nephritic, anti-inflammatory and cardiovascular protective activities. In this study, we investigated the effect of glabridin on dendritic cells, which play an essential role in innate and adaptive immune responses. ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2010.06.025
更新日期:2010-10-01 00:00:00
abstract::Extracellular adenosine triphosphate (ATP) is sequentially dephosphorylated by two ectoenzymes: CD39/nucleotidase triphosphate dephosphorylase (ENTPD) and CD73/5'-ectonucleotidase (5'-NT). Adenosine, its notable metabolite, may elicit potent anti-inflammatory responses. We examined whether the CD39-adenosinergic axis ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2013.07.014
更新日期:2013-11-01 00:00:00
abstract:AIMS:Immune mediated liver injury includes activation of different immune pathways that requires various modalities to control their consequences. The current study involves evaluation of retinoic acid (RA) modulatory effects on immune responses induced in concanavalin A (ConA) model of acute hepatitis. MAIN METHODS:M...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105808
更新日期:2019-10-01 00:00:00
abstract::This report sought to establish the mechanistic role of sirtuin-1 (Sirt1), a NAD+-dependent deacetylase in the modulation of primary biliary cholangitis (PBC) pathogenesis. 64 PBC patients (diagnosed based on practice guidelines for American Association for the Study of Liver Diseases) and 60 healthy controls were inc...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106386
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:Mesenchymal Stem Cells (MSCs) can interact with and modulate the functions of all immune cells, suppressing both the innate and adaptive immune responses. Currently, most of the in vitro studies which have led to the description of MSC properties have resulted from MSC culture in the presence of fetal bovine se...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.106172
更新日期:2020-02-01 00:00:00
abstract::B1 kinin receptor (B1R) is up-regulated by endotoxins and thus may represent a therapeutic target in sepsis. We investigated the expression and role of B1R and B2R in the acute phase of lipopolysaccharide (LPS)-induced endotoxin shock in C57BL/6 mice (WT) and B1R and B2R knock out mice (B1KO, B2KO). B1R mRNA was enhan...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2007.08.008
更新日期:2008-02-01 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is a severe complication of systemic lupus erythematosus (SLE), with unclear etiopathogenesis. We evaluated the role of macrophage migration inhibitory factor (MIF), which has been implicated in idiopathic pulmonary hypertension (PH), in SLE-associated PAH. Circulating MIF was mea...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105874
更新日期:2019-11-01 00:00:00
abstract::We can now declare that the clinical goal of immune intervention as a therapeutic strategy for neoplastic, infectious, autoimmune and inflammatory diseases, has been achieved and in many instances obtained regulatory approval. Although, interest in and optimism for this approach has fluctuated, in the last 20years, im...
journal_title:International immunopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.intimp.2016.02.025
更新日期:2016-08-01 00:00:00
abstract::The medical lead, so-called Cumaside, was created on the basis of triterpene oligoglycosides from the Far-Eastern edible sea cucumber (holothurian) Cucumaria japonica and its immunomodulatory properties were studied. The haemolytic activity of Cumaside was significantly reduced in comparison with original glycosides d...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2006.01.017
更新日期:2006-07-01 00:00:00