Imiquimod-related dermatitis is mainly mediated by mast cell degranulation via Mas-related G-protein coupled receptor B2.


:While imiquimod (IMQ) has been widely used in dermatology, its side effect manifested as dermatitis couldn't be ignored. However, the underlying mechanism has not been fully understood. Considering the clinical features of IMQ-related dermatitis similar to pseudo-allergic reaction and the presence of large numbers of mast cell in tissues treated with IMQ, the possibility that IMQ-related dermatitis mediated by mast cell-specific Mas-related G protein-coupled receptor X2 (MRGPRX2) should be addressed. To investigate the role of MRGPRX2 in vivo, MrgprB2, the mice homology of human MRGPRX2, was detected in IMQ-induced dermatitis mouse model. Histopathological changes including mast cell degranulation and footpad swelling were assayed in wild-type and MrgprB2-/- mice. The results showed that IMQ application induced dermatitis and footpad swelling with inflammatory cells infiltration plus mast cell activation in the skin of wild-type mice but reduced significantly in MrgprB2-/- mice. Further, compared to wild-type mice, serum histamine and inflammatory cytokine levels were compromised in MrgprB2-/- mice treated with IMQ, while the serum IgE level didn't change significantly. In vitro studies, levels of mediators released from murine peritoneal mast cells (MPMCs) after IMQ treatment were increased in a dose-dependent manner, which were much mild in MPMCs from MrgprB2-/- mice. Intracellular Ca2+ concentration was increased in a dose dependent manner after IMQ treatment both in MrgprB2-HEK293 and MRGPRX2-HEK293 cells. Moreover, β-hexosaminidase released after IMQ treatment was blocked by siRNA directed at the MRGPRX2 receptor in LAD2 cells. In summary, MrgprB2 /MRGPRX2 mediate mast cell activation and participate in IMQ-related dermatitis.


Int Immunopharmacol


Hao Y,Peng B,Che D,Zheng Y,Kong S,Liu R,Shi J,Han H,Wang J,Cao J,Zhang Y,Gao J,He L,Geng S




Has Abstract


2020-04-01 00:00:00












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    abstract::This study aims to explore the protective effects and mechanisms of ulinastatin (UTI), which is a urinary trypsin inhibitor, of the renal interstitial fibrosis of rats subjected to unilateral ureteral obstruction (UUO). A total of 36 male Wistar rats were divided in random into three groups, namely, the sham operation...

    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Ning XH,Ge XF,Cui Y,An HX

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  • Anemoside B4 prevents acute ulcerative colitis through inhibiting of TLR4/NF-κB/MAPK signaling pathway.

    abstract::Anemoside B4 (B4) is a compound extracted from Pulsatilla chinensis(P. chinensis). Pharmacological studies have proved that it has certain anti-inflammatory activity. Acute ulcerative colitis (ulcerative colitis) is a non-specific inflammatory disease whose pathogenesis is not completely known, and there is no effecti...

    journal_title:International immunopharmacology

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  • LOX-1 and TLR4 affect each other and regulate the generation of ROS in A. fumigatus keratitis.

    abstract:PURPOSE:To explore the relationship between LOX-1 and TLR4 in Aspergillus fumigatus (A. fumigatus) keratitis. To determine LOX-1 and TLR4 can affect each other and regulate inflammation through regulation of the generation of reactive oxygen species (ROS) in A. fumigatus keratitis. METHODS:The cornea and abdominal cav...

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    pub_type: 杂志文章


    authors: Gao X,Zhao G,Li C,Lin J,Jiang N,Wang Q,Hu L,Xu Q,Peng X,He K,Zhu G

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    abstract::Bothrops snake venoms contain biologically active components, including L-amino acid oxidases (LAAO) that induce significant leukocyte accumulation at inflammatory sites characterized by early neutrophil infiltration. As it remains unclear how snake venoms modulate neutrophil activation and chemokine production, here ...

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    authors: Pereira-Crott LS,Casare-Ogasawara TM,Ambrosio L,Chaim LFP,de Morais FR,Cintra ACO,Canicoba NC,Tucci LFF,Torqueti MR,Sampaio SV,Marzocchi-Machado CM,Castro FA

    更新日期:2020-03-01 00:00:00

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    authors: He JM,Chen SC,Li RP,Yuan LX,Bao JM,Guo ML

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    authors: Haupt M,Kauschke V,Sender J,Kampschulte M,Kovtun A,Dürselen L,Heiss C,Lips KS

    更新日期:2015-11-01 00:00:00

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    更新日期:2019-09-01 00:00:00

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    authors: Yun CH,Jeon YJ,Yang Y,Ju HR,Han SH

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    pub_type: 杂志文章


    authors: Savion S,Gidon-Dabush S,Fein A,Torchinsky A,Toder V

    更新日期:2004-10-01 00:00:00

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    authors: Carvalho MUWB,Vendramini P,Kubo CA,Soreiro-Pereira PV,de Albuquerque RS,Antunes E,Condino-Neto A

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    pub_type: 杂志文章


    authors: Son CG,Shin JW,Cho JH,Cho CK,Yun CH,Chung W,Han SH

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    journal_title:International immunopharmacology

    pub_type: 杂志文章


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    更新日期:2006-11-01 00:00:00

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    更新日期:2010-04-01 00:00:00

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    abstract::Effects of a topical corticosteroid drug, diflucortolone valerate, on the mRNA expressions for four CC- and four CXC-chemokines, which have been reported to be associated with recruitment of different kinds of proinflammatory and inflammatory cells, were investigated by RT-PCR in mice with 2,4,6-trinitrochlorobenzene ...

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    pub_type: 杂志文章


    authors: Mitsui G,Hirano T,Niwano Y,Mitsui K,Ohara O,Yanagihara S,Kato M

    更新日期:2004-01-01 00:00:00

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    abstract::Cellular and molecular events in vascular smooth muscle cells (VSMC) from Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were investigated. SHR-derived VSMC showed increased proliferative capacity and MAP kinase levels in comparison with WKY-derived VSMC. Flow cytometry analysis revealed that progre...

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    更新日期:2009-07-01 00:00:00

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    authors: Ma QY,Huang DY,Zhang HJ,Chen J,Miller W,Chen XF

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    abstract::Acute graft-versus-host disease (aGVHD) is a major complication following transplantation, limiting the success of this therapy. Chitinase 3-like-1 (CHI3L1), a member of the glycosyl hydrolase 18 family, plays a critical role in bacterial infections, allergic disease and a variety of malignancies. Here, we investigate...

    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Li Z,Gu J,Liu J,Zhu Q,Lu H,Lu Y,Rao J,Lu L,Wang X

    更新日期:2017-05-01 00:00:00

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    abstract::Psoriasis is a chronic inflammatory skin disease featured by excessive proliferation of keratinocytes, clearly defined round erythema and dry, scaly plaques, long-term inflammatory cells infiltration in skin lesions. However, the physiopathological mechanism of psoriasis is still not clearly understood. Neuropeptides,...

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    pub_type: 杂志文章,评审


    authors: Zhang Y,Zhang H,Jiang B,Yan S,Lu J

    更新日期:2020-10-01 00:00:00

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    abstract::Flavonoids are a class of compounds that exist in nature with the structure of 2-phenyl-chromone. In Chinese traditional medicine, herbal drugs containing flavonoids are widely used for the treatment of inflammation, cardiovascular disease, tumor and so on. In this study, we investigated the anti-inflammatory effect a...

    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Xu J,Jia YY,Chen SR,Ye JT,Bu XZ,Hu Y,Ma YZ,Guo JL,Liu PQ

    更新日期:2013-04-01 00:00:00

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    abstract::A lipoteichoic acid-related molecule (OK-PSA) isolated from OK-432, a penicillin-killed Streptococcus pyogenes, is a potent inducer of Th1 cytokines, and elicits anti-cancer effect in tumor-bearing mice. Toll-like receptor (TLR) 4 is a member of the recently identified toll-like receptor family of proteins that has be...

    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Okamoto M,Oshikawa T,Ohe G,Nishikawa H,Furuichi S,Tano T,Moriya Y,Saito M,Sato M

    更新日期:2001-09-01 00:00:00

  • Absolute reduction of regulatory T cells and regulatory effect of short-term and low-dose IL-2 in polymyositis or dermatomyositis.

    abstract:AIM:The study aimed to investigate the changes in peripherallymphocyte and CD4+T subsets and to observe the regulatory effect of low-dose interleukin-2 (ld-IL2) on these cells in polymyositis or dermatomyositis (PM/DM). METHODS:Lymphocyte subsets (CD3+T, CD4+T, CD8+T, B and natural killer (NK) cells), CD4+T subsets (T...

    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Feng M,Guo H,Zhang C,Wang Y,Liang Z,Zhao X,Qin Y,Wu Y,Liu G,Gao C,Luo J

    更新日期:2019-12-01 00:00:00

  • miR-181a regulates Th17 cells distribution via up-regulated BCL-2 in primary biliary cholangitis.

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    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Song Y,Yang H,Jiang K,Wang BM,Lin R

    更新日期:2018-11-01 00:00:00

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    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Xie C,Jingjing W,Li X,Zeng F,Ma L,Li C,Wei Z,Peng A,Chen L

    更新日期:2014-08-01 00:00:00

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    abstract::Inflammation occurs as a result of acute trauma, invasion of the host by different pathogens, pathogen-associated molecular patterns (PAMPs) or chronic cellular stress generating damage-associated molecular patterns (DAMPs). Thus inflammation may occur under both sterile inflammatory conditions including certain cance...

    journal_title:International immunopharmacology

    pub_type: 杂志文章,评审


    authors: Kumar V

    更新日期:2019-08-01 00:00:00

  • Anti-neuroinflammatory effect of aurantiamide acetate from the marine fungus Aspergillus sp. SF-5921: inhibition of NF-κB and MAPK pathways in lipopolysaccharide-induced mouse BV2 microglial cells.

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    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Yoon CS,Kim DC,Lee DS,Kim KS,Ko W,Sohn JH,Yim JH,Kim YC,Oh H

    更新日期:2014-12-01 00:00:00

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    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Liu X,Chen N,Lin S,Liu M

    更新日期:2016-01-01 00:00:00

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    abstract::The frequency of different subsets of CD4(+)CXCR5(+) TFH cells and serum cytokine levels were analyzed in a total of 14 patients with newly diagnosed hepatitis B virus-associated membranous nephropathy (HBV-MN), 12 individuals with immune-tolerant HBV infection (HBV-IT) and 12 healthy controls (HC). Serum cytokine lev...

    journal_title:International immunopharmacology

    pub_type: 杂志文章


    authors: Liu Y,Zhao P,Qu Z,Ayana DA,Jiang Y

    更新日期:2014-09-01 00:00:00