Abstract:
:Objective: To develop a cost-effective and clinically usable therapy to treat full-thickness skin injuries. We accomplished this by preparing a viscoelastic hydrogel using polyethylene glycol (PEG)-modified platelet-free plasma (PEGylated PFP) combined with human adipose-derived stem cells (ASCs). Approach: PEGylated PFP hydrogels were prepared by polymerizing the liquid mixture of PEG and PFP±ASCs and gelled either by adding calcium chloride (CaCl2) or thrombin. Rheological and in vitro studies were performed to assess viscoelasticity and the ability of hydrogels to direct ASCs toward a vasculogenic phenotype, respectively. Finally, a pilot study evaluated the efficacy of hydrogels±ASCs using an athymic rat full-thickness skin wound model. Results: Hydrogels prepared within the range of 11 to 27 mM for CaCl2 or 5 to 12.5 U/mL for thrombin exhibited a storage modulus of ∼62 to 87 Pa and ∼47 to 92 Pa, respectively. The PEGylated PFP hydrogels directed ASCs to form network-like structures resembling vasculature, with a fourfold increase in perivascular specific genes that were confirmed by immunofluorescent staining. Hydrogels combined with ASCs exhibited an increase in blood vessel density when applied to excisional rat wounds compared with those treated with hydrogels (110.3 vs. 95.6 BV/mm2; p < 0.05). Furthermore, ASCs were identified in the perivascular region associated with newly forming blood vessels. Innovation: This study demonstrates that PFP modified with PEG along with ASCs can be used to prepare cost-effective stable hydrogels, at the bed-side, to treat extensive skin wounds. Conclusion: These results indicate that PEGylated plasma-based hydrogels combined with ASCs may be a potential regenerative therapy for full-thickness skin wounds.
journal_name
Adv Wound Care (New Rochelle)journal_title
Advances in wound careauthors
Natesan S,Stone R,Coronado RE,Wrice NL,Kowalczewski AC,Zamora DO,Christy RJdoi
10.1089/wound.2018.0844subject
Has Abstractpub_date
2019-07-01 00:00:00pages
323-340issue
7eissn
2162-1918issn
2162-1934pii
10.1089/wound.2018.0844journal_volume
8pub_type
杂志文章abstract::Objective: Full-thickness burn wounds require immediate coverage, and the primary clinical approaches comprise of skin allografts and autografts. The use of allografts is often temporary due to the antigenicity of allografts. In contrast, the availability of skin autografts may be limited in large burn injuries. In su...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.1108
更新日期:2020-05-28 00:00:00
abstract::Introduction: Chronic wounds that are difficult to heal are physical and financial burden to the sufferer and a challenge to the treating physician. Objective: The main purpose of this study was to develop a novel method of using bone marrow (BM) aspirate, either cultured or fresh, as early solution for healing of chr...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2016.0704
更新日期:2017-04-01 00:00:00
abstract::Objective: To develop a venous leg ulcer (VLU) risk stratification system for use in research and clinical practice. Approach: U.S. Wound Registry data were examined retrospectively and assigned an outcome. Bivariate analysis identified significant variables (p < 0.05) that were used to create a multivariable logistic...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.1038
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:Over the past decade, the development of high-throughput technologies for DNA and protein analysis has revolutionized the ways in which cells can be studied. Within a relatively short time frame, research has changed from studying individual genes and proteins to analyzing entire genomes and proteomes. APPRO...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2012.0379
更新日期:2013-11-01 00:00:00
abstract::Significance: Fetal wounds heal with a regenerative phenotype that is indistinguishable from surrounding skin with restored skin integrity. Compared to this benchmark, all postnatal wound healing is impaired and characterized by scar formation. The biologic basis of the fetal regenerative phenotype can serve as a road...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2014.0564
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:Oxygen is vital for healing wounds. It is intricately involved in numerous biological processes including cell proliferation, angiogenesis, and protein synthesis, which are required for restoration of tissue function and integrity. Adequate wound tissue oxygenation can trigger healing responses and favorably...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0319
更新日期:2012-12-01 00:00:00
abstract::Significance: Keloids are benign fibro-proliferative raised dermal lesions that spread beyond the original borders of the wound, continue to grow, rarely regress, and are the most common in pigmented individuals after an abnormal wound healing response. The current treatment failure and respective challenges involved ...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2019.1040
更新日期:2019-12-01 00:00:00
abstract::The concept of the military wound is not an easy entity to define as the wounds seen in conflict can be of many types: those caused by recognized or improvised weapon systems may have similarities to civilian wounds as well as the wounds soldiers sustain outside of battle. This article will focus on the current treatm...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2015.0653
更新日期:2016-10-01 00:00:00
abstract:SIGNIFICANCE:Re-establishment of a functional vascular network is a critical component of successful wound repair. One of the most potent pro-angiogenic agents is vascular endothelial growth factor (VEGF), which, from a basic science and pre-clinical perspective, seems ideal for the therapeutic stimulation of blood ves...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0348
更新日期:2013-04-01 00:00:00
abstract::Rationale: The Tight Skin 2 (Tsk2) mouse model of systemic sclerosis (SSc) has many features of human disease, including tight skin, excessive collagen deposition, alterations in the extracellular matrix (ECM), increased elastic fibers, and occurrence of antinuclear antibodies with age. A tight skin phenotype is obser...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2014.0529
更新日期:2014-09-01 00:00:00
abstract:: Objective: In recent years, reticulated open-cell foam-based closed-incision negative pressure therapy (ROCF-ciNPT) has shown effectiveness in management of various postoperative incisions. These dressings consist of a skin interface layer that absorbs fluid from the skin ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.0997
更新日期:2020-08-01 00:00:00
abstract::Significance: Revealing the basic mechanisms in the healing process and then regulating these processes for faster healing or to avoid negative outcomes such as infection or scarring are fundamental to wound research. The normal healing process is basically known, but to thoroughly understand the very complex aspects ...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0502
更新日期:2014-03-01 00:00:00
abstract::Significance: Regulation of cell adhesions during tissue repair is fundamentally important for cell migration, proliferation, and protein production. All cells interact with extracellular matrix proteins with cell surface integrin receptors that convey signals from the environment into the nucleus, regulating gene exp...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0436
更新日期:2014-12-01 00:00:00
abstract::Objective: The use of noncultured autologous stromal vascular fraction or clinical grade adipose-derived regenerative cells (ADRCs) is a promising strategy to promote wound healing and tissue repair. Nevertheless, issues regarding the optimal mode of administration remain unclear. The purpose of this study was to comp...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2015.0672
更新日期:2016-07-01 00:00:00
abstract::Objective: To merge scholarly activity into the curriculum developed for medical students electing a rotation in wound care and/or dermatology. Approach: The authors adapted the unique wound care curriculum developed for medical student rotators and residents to incorporate structured scholarly projects, opportunities...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2013.0504
更新日期:2014-05-01 00:00:00
abstract:SIGNIFICANCE:Scarring continues to present a significant clinical problem. Wound contraction leads to scarring and is mediated by myofibroblasts and contractile forces across the wound bed. Contracture formation can have a significant impact on the quality of life of the patient, particularly where function and appeara...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0378
更新日期:2013-05-01 00:00:00
abstract::Significance: During wound healing of the skin, keratinocytes should move over while still adhering to their underlying matrix. Thus, mechanistic insights into the wound-healing process require an understanding of the forms and functions of keratinocyte matrix adhesions, specifically focal contacts and hemidesmosomes,...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0489
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Many wounds are difficult to heal because of the large, complex community of microbes present within the wound. THE PROBLEM:Classical laboratory culture methods do not provide an accurate picture of the microbial interactions or representation of microorganisms within a wound. There is an inherent bias in d...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0303
更新日期:2012-06-01 00:00:00
abstract::Significance: Chronic skin ulcers, including venous, diabetic, and pressure ulcers, constitute a major health care burden, affecting 2-6 million people in the United States alone, with projected increases in incidence owing to the aging population and rising epidemic of diabetes. The ulcers are often accompanied by pa...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2018.0866
更新日期:2019-04-01 00:00:00
abstract::Objective: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently a pandemic. Although pulmonary health has been the primary focus of studies during the early days of COVID-19, development of a comprehensive understanding of this emergent disease requi...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2020.1309
更新日期:2021-02-01 00:00:00
abstract:SIGNIFICANCE:Proteases and their inhibitors contribute to the balance between extracellular matrix (ECM) degradation and deposition, creating an equilibrium that is essential for the timely and coordinated healing of cutaneous wounds. However, when this balance is disrupted, wounds are led into a state of chronicity ch...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0370
更新日期:2013-10-01 00:00:00
abstract::Chronic and hard-to-heal wounds are a tremendous burden on our healthcare system and impair the quality of life for millions of people. An emerging focus of regenerative medicine is the development of natural biomaterials that can stimulate tissue formation or repair by recreating the functional and structural propert...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2015.0652
更新日期:2015-08-01 00:00:00
abstract::Objective: To investigate the safety, feasibility, and acceptability of a novel treatment, intermittent electrical stimulation (IES), for preventing deep tissue injury (DTI) in different healthcare settings. Approach: Testing was conducted in an acute rehabilitation unit of a general hospital, a tertiary rehabilitatio...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2014.0569
更新日期:2015-03-01 00:00:00
abstract::Objective: Splinting full-thickness cutaneous wounds in mice has allowed for a humanized model of wound healing. Delineating the epithelial edge and assessing time to closure of these healing wounds via macroscopic visualization have remained a challenge. Approach: Double transgenic mice were created by crossbreeding ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2017.0772
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Adipose tissue is one of the richest sources of mesenchymal stem cells that exhibit an outstanding ability to regenerate skin. THE PROBLEM:Although the anatomical sites of adipose-derived stem cells (ASCs) in the body are relatively oxygen-deficient (i.e., 1%-5% oxygen content), ASCs are usually cultured un...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0312
更新日期:2012-08-01 00:00:00
abstract::Microtubules (MTs) are intracellular polymers that provide structure to the cell, serve as railways for intracellular transport, and regulate many cellular activities, including cell migration. The dynamicity and function of the MT cytoskeleton are determined in large part by its regulatory proteins, including the rec...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0827
更新日期:2019-03-01 00:00:00
abstract::Objective: To determine the utility of instillation negative pressure wound therapy (NPWT) in achieving eradication of infection and definitive wound closure in patients with infected left ventricular assist device (LVAD). Approach: A retrospective review was performed in a series of patients with infected and exposed...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0832
更新日期:2019-03-01 00:00:00
abstract::Objective: To investigate the caring burden and its determinants of primary informal caregiver of patients with chronic wound. Approach: A prospective cross-sectional study of 132 pairs of chronic wound care recipients and their informal caregivers was included. The characteristics of patients and their informal careg...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0873
更新日期:2019-09-01 00:00:00
abstract::Significance: Venous leg ulcers (VLUs) are susceptible to microbial invasion, and serious complications can result without the timely control of infection. Diagnosis of wound infection is primarily based on subjective clinical characteristics and patient-reported symptoms, and the treatment with antimicrobials has not...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2014.0535
更新日期:2015-01-01 00:00:00
abstract::Objective: To inform on the interim results of the Remede d'Or study, which is a prospective, multicenter, single-blind, randomized, controlled clinical study on the safety and efficacy of RMD-G1, a topical carbopol-based hydrogel with a fibronectin matrix whose active pharmaceutical ingredient is erythropoietin (EPO)...
journal_title:Advances in wound care
pub_type: 新闻
doi:10.1089/wound.2018.0808
更新日期:2019-10-01 00:00:00