Autologous Bone Marrow Aspirate Therapy for Skin Tissue Engineering and Tissue Regeneration.


:Introduction: Chronic wounds that are difficult to heal are physical and financial burden to the sufferer and a challenge to the treating physician. Objective: The main purpose of this study was to develop a novel method of using bone marrow (BM) aspirate, either cultured or fresh, as early solution for healing of chronic wounds and further reduce infection and tissue necrosis. Approach: In this case-control study conducted on 75 patients with chronic wounds, 50 patients with aspirate or cultured BM were used as cases and 25 with only daily saline dressings were used as controls. Results: Autologous BM aspirate either as fresh or cultured even without identification, isolation, and selective application of stem cells achieved significant reduction in the wound surface area when compared with control group. Innovation: The application of cultured BM cells had significantly higher percentage reduction of wound size compared to freshly applied BM aspirate; this reflects a high importance of stem cell therapy. Conclusion: The acceptability of the procedure among the patients was highly encouraging. The entire procedure was safe and without any complication.


Advances in wound care


Gupta GJ,Karki K,Jain P,Saxena AK




Has Abstract


2017-04-01 00:00:00














  • The Wound Healing Index for Predicting Venous Leg Ulcer Outcome.

    abstract::Objective: To develop a venous leg ulcer (VLU) risk stratification system for use in research and clinical practice. Approach: U.S. Wound Registry data were examined retrospectively and assigned an outcome. Bivariate analysis identified significant variables (p < 0.05) that were used to create a multivariable logistic...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Fife CE,Horn SD

    更新日期:2020-02-01 00:00:00

  • The Wound Microbiome: Modern Approaches to Examining the Role of Microorganisms in Impaired Chronic Wound Healing.

    abstract::Significance: Bacterial burden is believed to play a significant role in impaired wound healing of chronic wounds and the development of infection-related complications. The standard of care in the clinic relies upon cultivation-dependent methods to identify microorganisms. These assays are biased toward microorganism...

    journal_title:Advances in wound care

    pub_type: 评审


    authors: Misic AM,Gardner SE,Grice EA

    更新日期:2014-07-01 00:00:00

  • Absorption and Safety of Topically Applied Timolol for Treatment of Chronic Cutaneous Wounds.

    abstract::Objective: There are no safety or absorption studies to guide topical timolol therapy for treatment of chronic wounds. This study was undertaken to address this gap. Approach: A prospective, observational, cross-sectional comparative study of timolol plasma levels in patients after topical administration to a chronic ...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Gallegos AC,Davis MJ,Tchanque-Fossuo CN,West K,Eisentrout-Melton A,Peavy TR,Dixon RW,Patel RP,Dahle SE,Isseroff RR

    更新日期:2019-11-01 00:00:00

  • Safety and Feasibility of Intermittent Electrical Stimulation for the Prevention of Deep Tissue Injury.

    abstract::Objective: To investigate the safety, feasibility, and acceptability of a novel treatment, intermittent electrical stimulation (IES), for preventing deep tissue injury (DTI) in different healthcare settings. Approach: Testing was conducted in an acute rehabilitation unit of a general hospital, a tertiary rehabilitatio...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Ahmetović A,Mushahwar VK,Sommer R,Schnepf D,Kawasaki L,Warwaruk-Rogers R,Barlott T,Chong SL,Isaacson G,Kim S,Ferguson-Pell M,Stein RB,Ho C,Dukelow S,Chan KM

    更新日期:2015-03-01 00:00:00

  • PEGylated Platelet-Free Blood Plasma-Based Hydrogels for Full-Thickness Wound Regeneration.

    abstract::Objective: To develop a cost-effective and clinically usable therapy to treat full-thickness skin injuries. We accomplished this by preparing a viscoelastic hydrogel using polyethylene glycol (PEG)-modified platelet-free plasma (PEGylated PFP) combined with human adipose-derived stem cells (ASCs). Approach: PEGylated ...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Natesan S,Stone R,Coronado RE,Wrice NL,Kowalczewski AC,Zamora DO,Christy RJ

    更新日期:2019-07-01 00:00:00

  • In Situ Forming Nutritional and Temperature Sensitive Scaffold Improves the Esthetic Outcomes of Meshed Split-Thickness Skin Grafts in a Porcine Model.

    abstract::Objective: Full-thickness burn wounds require immediate coverage, and the primary clinical approaches comprise of skin allografts and autografts. The use of allografts is often temporary due to the antigenicity of allografts. In contrast, the availability of skin autografts may be limited in large burn injuries. In su...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Pourghadiri A,Alnojeidi H,Jalili R,Kilani RT,Nabai L,Ghahary A

    更新日期:2020-05-28 00:00:00

  • Cutaneous Manifestations of COVID-19: A Systematic Review.

    abstract::Objective: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently a pandemic. Although pulmonary health has been the primary focus of studies during the early days of COVID-19, development of a comprehensive understanding of this emergent disease requi...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Singh H,Kaur H,Singh K,Sen CK

    更新日期:2021-02-01 00:00:00

  • Chemokine Involvement in Fetal and Adult Wound Healing.

    abstract::Significance: Fetal wounds heal with a regenerative phenotype that is indistinguishable from surrounding skin with restored skin integrity. Compared to this benchmark, all postnatal wound healing is impaired and characterized by scar formation. The biologic basis of the fetal regenerative phenotype can serve as a road...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: Balaji S,Watson CL,Ranjan R,King A,Bollyky PL,Keswani SG

    更新日期:2015-11-01 00:00:00

  • Integrins in Wound Healing.

    abstract::Significance: Regulation of cell adhesions during tissue repair is fundamentally important for cell migration, proliferation, and protein production. All cells interact with extracellular matrix proteins with cell surface integrin receptors that convey signals from the environment into the nucleus, regulating gene exp...

    journal_title:Advances in wound care

    pub_type: 评审


    authors: Koivisto L,Heino J,Häkkinen L,Larjava H

    更新日期:2014-12-01 00:00:00

  • Association Between Microbial Bioburden and Healing Outcomes in Venous Leg Ulcers: A Review of the Evidence.

    abstract::Significance: Venous leg ulcers (VLUs) are susceptible to microbial invasion, and serious complications can result without the timely control of infection. Diagnosis of wound infection is primarily based on subjective clinical characteristics and patient-reported symptoms, and the treatment with antimicrobials has not...

    journal_title:Advances in wound care

    pub_type: 评审


    authors: Tuttle MS

    更新日期:2015-01-01 00:00:00

  • Wound Healing Finally Enters the Age of Molecular Diagnostic Medicine.

    abstract:BACKGROUND:Many wounds are difficult to heal because of the large, complex community of microbes present within the wound. THE PROBLEM:Classical laboratory culture methods do not provide an accurate picture of the microbial interactions or representation of microorganisms within a wound. There is an inherent bias in d...

    journal_title:Advances in wound care

    pub_type: 评审


    authors: Tatum OL,Dowd SE

    更新日期:2012-06-01 00:00:00

  • Breast Reconstruction After Radiotherapy.

    abstract::It was once common practice in the treatment of breast cancer for total mastectomy and axillary lymph node dissection to be conventionally performed. However, breast-conserving surgery is increasingly being performed with marked improvement in a multidisciplinary treatment approach, including surgery, chemotherapy, ir...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Yoshimoto H,Hamuy R

    更新日期:2014-01-01 00:00:00

  • Adjustability and Adaptability Are Critical Characteristics of Pediatric Support Surfaces.

    abstract::Significance: Pressure ulcers (PUs) in newborns and children are remarkably different from those in adults, both in their possible causal factors and in the etiology and biomechanical pathways for tissue damage. Recent Advances: Pediatric muscle and fat tissue structures are overall softer than those of adults, making...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: Levy A,Kopplin K,Gefen A

    更新日期:2015-10-01 00:00:00

  • An Improved Humanized Mouse Model for Excisional Wound Healing Using Double Transgenic Mice.

    abstract::Objective: Splinting full-thickness cutaneous wounds in mice has allowed for a humanized model of wound healing. Delineating the epithelial edge and assessing time to closure of these healing wounds via macroscopic visualization have remained a challenge. Approach: Double transgenic mice were created by crossbreeding ...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Hu MS,Cheng J,Borrelli MR,Leavitt T,Walmsley GG,Zielins ER,Hong WX,Cheung ATM,Duscher D,Maan ZN,Irizarry DM,Stephan B,Parsa FD,Wan DC,Gurtner GC,Lorenz HP,Longaker MT

    更新日期:2018-01-01 00:00:00

  • Effect of Flightless I Expression on Epidermal Stem Cell Niche During Wound Repair.

    abstract::Objective: Activation of epidermal stem cells (EpSCs) from their quiescent niche is an integral component of wound reepithelialization and involves Wnt/β-catenin (β-Cat) signaling and remodeling of the actin cytoskeleton. The aim of this study was to investigate the effect of Flightless I (Flii), a cytoskeletal protei...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Yang GN,Strudwick XL,Bonder C,Kopecki Z,Cowin AJ

    更新日期:2020-04-01 00:00:00

  • Toll-Like Receptor Signaling in Burn Wound Healing and Scarring.

    abstract::Significance: Damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) emanate from burn-injured tissue and enter systemic circulation. Locally and systemically, they activate pattern-recognition receptors, including toll-like receptors (TLRs), to stimulate cytokine secretion, wh...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: D'Arpa P,Leung KP

    更新日期:2017-10-01 00:00:00

  • Hypoxic Culturing Enhances the Wound-Healing Potential of Adipose-Derived Stem Cells.

    abstract:BACKGROUND:Adipose tissue is one of the richest sources of mesenchymal stem cells that exhibit an outstanding ability to regenerate skin. THE PROBLEM:Although the anatomical sites of adipose-derived stem cells (ASCs) in the body are relatively oxygen-deficient (i.e., 1%-5% oxygen content), ASCs are usually cultured un...

    journal_title:Advances in wound care

    pub_type: 评审


    authors: Kim WS,Sung JH

    更新日期:2012-08-01 00:00:00

  • Adipose-Derived Regenerative Cell Therapy for Burn Wound Healing: A Comparison of Two Delivery Methods.

    abstract::Objective: The use of noncultured autologous stromal vascular fraction or clinical grade adipose-derived regenerative cells (ADRCs) is a promising strategy to promote wound healing and tissue repair. Nevertheless, issues regarding the optimal mode of administration remain unclear. The purpose of this study was to comp...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Foubert P,Gonzalez AD,Teodosescu S,Berard F,Doyle-Eisele M,Yekkala K,Tenenhaus M,Fraser JK

    更新日期:2016-07-01 00:00:00

  • Full-Thickness Thermal Injury Delays Wound Closure in a Murine Model.

    abstract::Objective: The contemporary treatment of a full-thickness burn consists of early eschar excision followed by immediate closure of the open wound using autologous skin. However, most animal models study burn wound healing with the persistence of the burn eschar. Our goal is to characterize a murine model of burn eschar...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Wu JC,Rose LF,Christy RJ,Leung KP,Chan RK

    更新日期:2015-02-01 00:00:00

  • Therapeutic Applications of Extracellular Matrix.

    abstract::Chronic and hard-to-heal wounds are a tremendous burden on our healthcare system and impair the quality of life for millions of people. An emerging focus of regenerative medicine is the development of natural biomaterials that can stimulate tissue formation or repair by recreating the functional and structural propert...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Hocking DC

    更新日期:2015-08-01 00:00:00

  • Instillation Negative Pressure Wound Therapy: A Role for Infected LVAD Salvage.

    abstract::Objective: To determine the utility of instillation negative pressure wound therapy (NPWT) in achieving eradication of infection and definitive wound closure in patients with infected left ventricular assist device (LVAD). Approach: A retrospective review was performed in a series of patients with infected and exposed...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Hodson T,West JM,Poteet SJ,Lee PH,Valerio IL

    更新日期:2019-03-01 00:00:00

  • Tight Skin 2 Mice Exhibit Delayed Wound Healing Caused by Increased Elastic Fibers in Fibrotic Skin.

    abstract::Rationale: The Tight Skin 2 (Tsk2) mouse model of systemic sclerosis (SSc) has many features of human disease, including tight skin, excessive collagen deposition, alterations in the extracellular matrix (ECM), increased elastic fibers, and occurrence of antinuclear antibodies with age. A tight skin phenotype is obser...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Long KB,Burgwin CM,Huneke R,Artlett CM,Blankenhorn EP

    更新日期:2014-09-01 00:00:00

  • Medicare Coverage: You Cannot Play the Game If You Do Not Know the Rules.

    abstract::WOUND CARE STAKEHOLDERS SHOULD REMEMBER THAT MEDICARE REIMBURSEMENT REQUIRES THREE PARTS: a relevant code, a published Medicare payment rate, and positive coverage or coverage based upon medical necessity. Qualified healthcare professionals, scientists, and manufacturers should establish a monthly routine, where they ...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Schaum KD

    更新日期:2013-12-01 00:00:00

  • In Vitro and Ex Vivo Models for Functional Testing of Therapeutic Anti-scarring Drug Targets in Keloids.

    abstract::Significance: Keloids are benign fibro-proliferative raised dermal lesions that spread beyond the original borders of the wound, continue to grow, rarely regress, and are the most common in pigmented individuals after an abnormal wound healing response. The current treatment failure and respective challenges involved ...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: Sharma JR,Lebeko M,Kidzeru EB,Khumalo NP,Bayat A

    更新日期:2019-12-01 00:00:00

  • Current Advancements and Strategies in Tissue Engineering for Wound Healing: A Comprehensive Review.

    abstract::Significance: With an aging population leading to an increase in diabetes and associated cutaneous wounds, there is a pressing clinical need to improve wound-healing therapies. Recent Advances: Tissue engineering approaches for wound healing and skin regeneration have been developed over the past few decades. A review...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: Ho J,Walsh C,Yue D,Dardik A,Cheema U

    更新日期:2017-06-01 00:00:00

  • Skin Biomechanics and miRNA Expression Following Chronic UVB Irradiation.

    abstract::Objective: Exposure to ultraviolet (UV) light from the sun is known to accelerate the skin aging process and leads to significant alterations in skin biomechanics; however, the molecular mechanisms by which chronic UVB affects biomechanical properties of the skin have not been well described. Approach: A murine model ...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Blackstone BN,Wilgus TA,Roy S,Wulff BC,Powell HM

    更新日期:2020-03-01 00:00:00

  • Proteases and Delayed Wound Healing.

    abstract:SIGNIFICANCE:Proteases and their inhibitors contribute to the balance between extracellular matrix (ECM) degradation and deposition, creating an equilibrium that is essential for the timely and coordinated healing of cutaneous wounds. However, when this balance is disrupted, wounds are led into a state of chronicity ch...

    journal_title:Advances in wound care

    pub_type: 评审


    authors: McCarty SM,Percival SL

    更新日期:2013-10-01 00:00:00

  • The Management of Combat Wounds: The British Military Experience.

    abstract::The concept of the military wound is not an easy entity to define as the wounds seen in conflict can be of many types: those caused by recognized or improvised weapon systems may have similarities to civilian wounds as well as the wounds soldiers sustain outside of battle. This article will focus on the current treatm...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: Jeffery SL

    更新日期:2016-10-01 00:00:00

  • Three-Dimensional Printing and Cell Therapy for Wound Repair.

    abstract::Significance: Skin tissue damage is a major challenge and a burden on healthcare systems, from burns and other trauma to diabetes and vascular disease. Although the biological complexities are relatively well understood, appropriate repair mechanisms are scarce. Three-dimensional bioprinting is a layer-based approach ...

    journal_title:Advances in wound care

    pub_type: 杂志文章,评审


    authors: van Kogelenberg S,Yue Z,Dinoro JN,Baker CS,Wallace GG

    更新日期:2018-05-01 00:00:00

  • An Introduction to Functional Genomics and Systems Biology.

    abstract:OBJECTIVE:Over the past decade, the development of high-throughput technologies for DNA and protein analysis has revolutionized the ways in which cells can be studied. Within a relatively short time frame, research has changed from studying individual genes and proteins to analyzing entire genomes and proteomes. APPRO...

    journal_title:Advances in wound care

    pub_type: 杂志文章


    authors: Bunnik EM,Le Roch KG

    更新日期:2013-11-01 00:00:00