Abstract:
OBJECTIVE:Over the past decade, the development of high-throughput technologies for DNA and protein analysis has revolutionized the ways in which cells can be studied. Within a relatively short time frame, research has changed from studying individual genes and proteins to analyzing entire genomes and proteomes. APPROACH:In this article, we summarize the technologies and concepts that form the basis of this functional genomics approach. RESULTS:Microarray and next-generation sequencing technologies have allowed researchers to investigate many different aspects of the cell, including DNA mutations, histone modifications, DNA methylation, chromatin structure, transcription, and translation on a genome-wide level. In addition, mass spectrometry technologies have undergone significant development and currently enable us to globally profile protein levels, protein-protein interactions, post-translational protein modifications, and metabolites. INNOVATION AND CONCLUSION:The integration of information from the various processes that occur within a cell provides a more complete picture of how genes give rise to biological functions, and will ultimately help us to understand the biology of organisms, in both health and disease.
journal_name
Adv Wound Care (New Rochelle)journal_title
Advances in wound careauthors
Bunnik EM,Le Roch KGdoi
10.1089/wound.2012.0379subject
Has Abstractpub_date
2013-11-01 00:00:00pages
490-498issue
9eissn
2162-1918issn
2162-1934pii
10.1089/wound.2012.0379journal_volume
2pub_type
杂志文章abstract::Objective: Splinting full-thickness cutaneous wounds in mice has allowed for a humanized model of wound healing. Delineating the epithelial edge and assessing time to closure of these healing wounds via macroscopic visualization have remained a challenge. Approach: Double transgenic mice were created by crossbreeding ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2017.0772
更新日期:2018-01-01 00:00:00
abstract::Significance: Biofilms in vivo are small densely packed aggregations of microbes that are highly resistant to host immune responses and treatment. They attach to each other and to nearby surfaces. Biofilms are difficult to study and identify in a clinical setting as their quantification necessitates the use of advance...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2020.1193
更新日期:2021-01-01 00:00:00
abstract:SIGNIFICANCE:Scarring continues to present a significant clinical problem. Wound contraction leads to scarring and is mediated by myofibroblasts and contractile forces across the wound bed. Contracture formation can have a significant impact on the quality of life of the patient, particularly where function and appeara...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0378
更新日期:2013-05-01 00:00:00
abstract::Significance: Chronic skin ulcers, including venous, diabetic, and pressure ulcers, constitute a major health care burden, affecting 2-6 million people in the United States alone, with projected increases in incidence owing to the aging population and rising epidemic of diabetes. The ulcers are often accompanied by pa...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2018.0866
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Oxygen is vital for healing wounds. It is intricately involved in numerous biological processes including cell proliferation, angiogenesis, and protein synthesis, which are required for restoration of tissue function and integrity. Adequate wound tissue oxygenation can trigger healing responses and favorably...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0319
更新日期:2012-12-01 00:00:00
abstract::Significance: Revealing the basic mechanisms in the healing process and then regulating these processes for faster healing or to avoid negative outcomes such as infection or scarring are fundamental to wound research. The normal healing process is basically known, but to thoroughly understand the very complex aspects ...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0502
更新日期:2014-03-01 00:00:00
abstract::Significance: During wound healing of the skin, keratinocytes should move over while still adhering to their underlying matrix. Thus, mechanistic insights into the wound-healing process require an understanding of the forms and functions of keratinocyte matrix adhesions, specifically focal contacts and hemidesmosomes,...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0489
更新日期:2014-03-01 00:00:00
abstract::Significance: Damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) emanate from burn-injured tissue and enter systemic circulation. Locally and systemically, they activate pattern-recognition receptors, including toll-like receptors (TLRs), to stimulate cytokine secretion, wh...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2017.0733
更新日期:2017-10-01 00:00:00
abstract::Objective: Full-thickness burn wounds require immediate coverage, and the primary clinical approaches comprise of skin allografts and autografts. The use of allografts is often temporary due to the antigenicity of allografts. In contrast, the availability of skin autografts may be limited in large burn injuries. In su...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.1108
更新日期:2020-05-28 00:00:00
abstract::Significance: Fibrosis-related events play a part in the pathogenesis or failure of treatment of virtually all the blinding diseases around the world, and also account for over 40% of all deaths. It is well established that the eye and other tissues of some group of patients, for example Afro-Caribbean people, scar wo...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2015.0677
更新日期:2016-09-01 00:00:00
abstract::Introduction: Chronic wounds that are difficult to heal are physical and financial burden to the sufferer and a challenge to the treating physician. Objective: The main purpose of this study was to develop a novel method of using bone marrow (BM) aspirate, either cultured or fresh, as early solution for healing of chr...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2016.0704
更新日期:2017-04-01 00:00:00
abstract::Significance: Advances in field-based trauma care, surgical techniques, and protective equipment have collectively facilitated the survival of a historically large number of service members (SMs) following combat trauma, although many sustained significant composite tissue injuries to the extremities, including limb l...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2016.0714
更新日期:2017-08-01 00:00:00
abstract::Significance: Multispecies microbial biofilms may contribute to wound chronicity by derailing the inherent reparative process of the host tissue. In the biofilm form, bacteria are encased within an extracellular polymeric substance and become recalcitrant to antimicrobials and host defenses. For biofilms of relevance ...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2014.0587
更新日期:2015-07-01 00:00:00
abstract:PROBLEM:Lower extremity ulcers (venous, diabetic) are often unresponsive to standard treatment. Various systemic and local cellular, vascular, and anatomical factors can result in nonhealing wounds that are refractory to normal healing processes and standard care. SOLUTION:Several published wound care guidelines stron...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2011.0290
更新日期:2012-02-01 00:00:00
abstract::Objective: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently a pandemic. Although pulmonary health has been the primary focus of studies during the early days of COVID-19, development of a comprehensive understanding of this emergent disease requi...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2020.1309
更新日期:2021-02-01 00:00:00
abstract::Objective: Epidermal CD34+ stem cells located in the hair follicle (HF) bulge area are capable of inducing HF neogenesis and enhancing wound healing after transplantation. In this study, we observed CD34+ cells derived from blood directly participate in dermal regeneration during full-thickness excisional wound healin...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.0974
更新日期:2020-05-01 00:00:00
abstract:SIGNIFICANCE:Re-establishment of a functional vascular network is a critical component of successful wound repair. One of the most potent pro-angiogenic agents is vascular endothelial growth factor (VEGF), which, from a basic science and pre-clinical perspective, seems ideal for the therapeutic stimulation of blood ves...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0348
更新日期:2013-04-01 00:00:00
abstract::Significance: Broad-spectrum metalloproteinase (MMP) reduction along with inherent aspects of an extracellular matrix (ECM) dressing can bring about improved wound healing outcomes and shorter treatment duration. Initial reports of clinical effectiveness of a new ovine-based collagen extracellular matrix (CECM) dressi...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2015.0660
更新日期:2016-01-01 00:00:00
abstract::Objective: There are no safety or absorption studies to guide topical timolol therapy for treatment of chronic wounds. This study was undertaken to address this gap. Approach: A prospective, observational, cross-sectional comparative study of timolol plasma levels in patients after topical administration to a chronic ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.0970
更新日期:2019-11-01 00:00:00
abstract::Microtubules (MTs) are intracellular polymers that provide structure to the cell, serve as railways for intracellular transport, and regulate many cellular activities, including cell migration. The dynamicity and function of the MT cytoskeleton are determined in large part by its regulatory proteins, including the rec...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0827
更新日期:2019-03-01 00:00:00
abstract:SIGNIFICANCE:Epidermal growth factor (EGF) and EGF receptor (EGFR) play an essential role in wound healing through stimulating epidermal and dermal regeneration. The development of new therapies for enhancing wound healing has included the use of EGF. In addition, EGFR inhibitors (EGFRis) have become a therapeutic opti...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0326
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Many wounds are difficult to heal because of the large, complex community of microbes present within the wound. THE PROBLEM:Classical laboratory culture methods do not provide an accurate picture of the microbial interactions or representation of microorganisms within a wound. There is an inherent bias in d...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0303
更新日期:2012-06-01 00:00:00
abstract::Objective: To inform on the interim results of the Remede d'Or study, which is a prospective, multicenter, single-blind, randomized, controlled clinical study on the safety and efficacy of RMD-G1, a topical carbopol-based hydrogel with a fibronectin matrix whose active pharmaceutical ingredient is erythropoietin (EPO)...
journal_title:Advances in wound care
pub_type: 新闻
doi:10.1089/wound.2018.0808
更新日期:2019-10-01 00:00:00
abstract:SIGNIFICANCE:Proteases and their inhibitors contribute to the balance between extracellular matrix (ECM) degradation and deposition, creating an equilibrium that is essential for the timely and coordinated healing of cutaneous wounds. However, when this balance is disrupted, wounds are led into a state of chronicity ch...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0370
更新日期:2013-10-01 00:00:00
abstract::Significance: With an aging population leading to an increase in diabetes and associated cutaneous wounds, there is a pressing clinical need to improve wound-healing therapies. Recent Advances: Tissue engineering approaches for wound healing and skin regeneration have been developed over the past few decades. A review...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2016.0723
更新日期:2017-06-01 00:00:00
abstract::Objective: Exposure to ultraviolet (UV) light from the sun is known to accelerate the skin aging process and leads to significant alterations in skin biomechanics; however, the molecular mechanisms by which chronic UVB affects biomechanical properties of the skin have not been well described. Approach: A murine model ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.1034
更新日期:2020-03-01 00:00:00
abstract::Objective: Wound healing is a complex process involving the dynamic interplay of various types of cells and bioactive factors. Impaired wound healing is characterized by a loss in synchronization of the process, resulting in non-healing chronic wounds. Human amniotic membrane (AM) has been shown to be effective in the...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0931
更新日期:2019-08-01 00:00:00
abstract::Significance: Angiogenesis is an important phenomenon involved in the healing of chronic wounds, and it is mainly mediated by the release of vascular endothelial growth factor (VEGF) from endothelial cells. Electrical stimulation (ES) is a well-documented treatment used to assist the healing of chronic wounds. Due to ...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0427
更新日期:2014-02-01 00:00:00
abstract::Objective: Activation of epidermal stem cells (EpSCs) from their quiescent niche is an integral component of wound reepithelialization and involves Wnt/β-catenin (β-Cat) signaling and remodeling of the actin cytoskeleton. The aim of this study was to investigate the effect of Flightless I (Flii), a cytoskeletal protei...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0884
更新日期:2020-04-01 00:00:00
abstract::Objective: To investigate the safety, feasibility, and acceptability of a novel treatment, intermittent electrical stimulation (IES), for preventing deep tissue injury (DTI) in different healthcare settings. Approach: Testing was conducted in an acute rehabilitation unit of a general hospital, a tertiary rehabilitatio...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2014.0569
更新日期:2015-03-01 00:00:00