Abstract:
:Significance: Venous leg ulcers (VLUs) are susceptible to microbial invasion, and serious complications can result without the timely control of infection. Diagnosis of wound infection is primarily based on subjective clinical characteristics and patient-reported symptoms, and the treatment with antimicrobials has not consistently shown improvement in healing outcomes. This is a review of studies using bacterial cultures and/or new molecular-based methods associating microbial bioburden with healing outcomes in VLU patients, with the goal of guiding future studies to better determine significant patterns of microbial involvement in chronic wounds. Recent Advances: Studies reviewed here use cultivation-based identification of bacteria and next-generation sequencing of the bacterial 16S rRNA gene to gain insight into microbial bioburden in VLUs. Further application of sophisticated DNA sequencing and bioinformatic analyses has the potential to revolutionize our ability to further discern, with high resolution, complex microbial communities in chronic wounds. Critical Issues: Few previous studies of microbial bioburden in VLUs have incorporated the knowledge of clinical treatments, which includes close monitoring of patients' symptoms and responses to therapy. Thus, wound care practitioners are currently without evidence-based guidance for the diagnosis and treatment of wound infections. Future Directions: Clinically relevant breakthroughs are possible by combining advanced microbial detection techniques with improved study designs that reflect clinical practices. Well-designed longitudinal studies have great potential to lead to better evidence-based diagnosis of chronic wounds. A greater understanding of microbial bioburden in chronic wounds is likely to lead to better therapies that speed healing and prevent wound infection without risking the development of antimicrobial resistance.
journal_name
Adv Wound Care (New Rochelle)journal_title
Advances in wound careauthors
Tuttle MSdoi
10.1089/wound.2014.0535subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1-11issue
1eissn
2162-1918issn
2162-1934pii
10.1089/wound.2014.0535journal_volume
4pub_type
评审abstract::Chronic and hard-to-heal wounds are a tremendous burden on our healthcare system and impair the quality of life for millions of people. An emerging focus of regenerative medicine is the development of natural biomaterials that can stimulate tissue formation or repair by recreating the functional and structural propert...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2015.0652
更新日期:2015-08-01 00:00:00
abstract::Significance: Angiogenesis is an important phenomenon involved in the healing of chronic wounds, and it is mainly mediated by the release of vascular endothelial growth factor (VEGF) from endothelial cells. Electrical stimulation (ES) is a well-documented treatment used to assist the healing of chronic wounds. Due to ...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0427
更新日期:2014-02-01 00:00:00
abstract::Objective: To investigate the caring burden and its determinants of primary informal caregiver of patients with chronic wound. Approach: A prospective cross-sectional study of 132 pairs of chronic wound care recipients and their informal caregivers was included. The characteristics of patients and their informal careg...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0873
更新日期:2019-09-01 00:00:00
abstract:SIGNIFICANCE:Postnatal vasculogenesis mediated via endothelial progenitor cells (EPCs) contributes to re-endothelialization and augments neovascularization after ischemia and tissue injury, providing a novel therapeutic application. However, controversy exists with respect to the origin, identification, and contributio...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0398
更新日期:2013-07-01 00:00:00
abstract::Objective: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently a pandemic. Although pulmonary health has been the primary focus of studies during the early days of COVID-19, development of a comprehensive understanding of this emergent disease requi...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2020.1309
更新日期:2021-02-01 00:00:00
abstract::Significance: Skin tissue damage is a major challenge and a burden on healthcare systems, from burns and other trauma to diabetes and vascular disease. Although the biological complexities are relatively well understood, appropriate repair mechanisms are scarce. Three-dimensional bioprinting is a layer-based approach ...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2017.0752
更新日期:2018-05-01 00:00:00
abstract:: Objective: In recent years, reticulated open-cell foam-based closed-incision negative pressure therapy (ROCF-ciNPT) has shown effectiveness in management of various postoperative incisions. These dressings consist of a skin interface layer that absorbs fluid from the skin ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.0997
更新日期:2020-08-01 00:00:00
abstract::Objective: The use of noncultured autologous stromal vascular fraction or clinical grade adipose-derived regenerative cells (ADRCs) is a promising strategy to promote wound healing and tissue repair. Nevertheless, issues regarding the optimal mode of administration remain unclear. The purpose of this study was to comp...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2015.0672
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Adipose tissue is one of the richest sources of mesenchymal stem cells that exhibit an outstanding ability to regenerate skin. THE PROBLEM:Although the anatomical sites of adipose-derived stem cells (ASCs) in the body are relatively oxygen-deficient (i.e., 1%-5% oxygen content), ASCs are usually cultured un...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0312
更新日期:2012-08-01 00:00:00
abstract:PROBLEM:Lower extremity ulcers (venous, diabetic) are often unresponsive to standard treatment. Various systemic and local cellular, vascular, and anatomical factors can result in nonhealing wounds that are refractory to normal healing processes and standard care. SOLUTION:Several published wound care guidelines stron...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2011.0290
更新日期:2012-02-01 00:00:00
abstract::Significance: For over 30 years, there has been sustained interest in the development of mathematical models for investigating the complex mechanisms underlying each stage of the wound healing process. Despite the immense associated challenges, such models have helped usher in a paradigm shift in wound healing researc...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.1132
更新日期:2020-09-11 00:00:00
abstract::Significance: Regulation of cell adhesions during tissue repair is fundamentally important for cell migration, proliferation, and protein production. All cells interact with extracellular matrix proteins with cell surface integrin receptors that convey signals from the environment into the nucleus, regulating gene exp...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0436
更新日期:2014-12-01 00:00:00
abstract::Significance: Revealing the basic mechanisms in the healing process and then regulating these processes for faster healing or to avoid negative outcomes such as infection or scarring are fundamental to wound research. The normal healing process is basically known, but to thoroughly understand the very complex aspects ...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0502
更新日期:2014-03-01 00:00:00
abstract::Significance: Multispecies microbial biofilms may contribute to wound chronicity by derailing the inherent reparative process of the host tissue. In the biofilm form, bacteria are encased within an extracellular polymeric substance and become recalcitrant to antimicrobials and host defenses. For biofilms of relevance ...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2014.0587
更新日期:2015-07-01 00:00:00
abstract::Significance: During wound healing of the skin, keratinocytes should move over while still adhering to their underlying matrix. Thus, mechanistic insights into the wound-healing process require an understanding of the forms and functions of keratinocyte matrix adhesions, specifically focal contacts and hemidesmosomes,...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2013.0489
更新日期:2014-03-01 00:00:00
abstract::Significance: Bacterial burden is believed to play a significant role in impaired wound healing of chronic wounds and the development of infection-related complications. The standard of care in the clinic relies upon cultivation-dependent methods to identify microorganisms. These assays are biased toward microorganism...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0397
更新日期:2014-07-01 00:00:00
abstract::Significance: Biofilms in vivo are small densely packed aggregations of microbes that are highly resistant to host immune responses and treatment. They attach to each other and to nearby surfaces. Biofilms are difficult to study and identify in a clinical setting as their quantification necessitates the use of advance...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2020.1193
更新日期:2021-01-01 00:00:00
abstract:SIGNIFICANCE:Scarring continues to present a significant clinical problem. Wound contraction leads to scarring and is mediated by myofibroblasts and contractile forces across the wound bed. Contracture formation can have a significant impact on the quality of life of the patient, particularly where function and appeara...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2012.0378
更新日期:2013-05-01 00:00:00
abstract::Rationale: The Tight Skin 2 (Tsk2) mouse model of systemic sclerosis (SSc) has many features of human disease, including tight skin, excessive collagen deposition, alterations in the extracellular matrix (ECM), increased elastic fibers, and occurrence of antinuclear antibodies with age. A tight skin phenotype is obser...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2014.0529
更新日期:2014-09-01 00:00:00
abstract::Objective: To inform on the interim results of the Remede d'Or study, which is a prospective, multicenter, single-blind, randomized, controlled clinical study on the safety and efficacy of RMD-G1, a topical carbopol-based hydrogel with a fibronectin matrix whose active pharmaceutical ingredient is erythropoietin (EPO)...
journal_title:Advances in wound care
pub_type: 新闻
doi:10.1089/wound.2018.0808
更新日期:2019-10-01 00:00:00
abstract::Significance: Damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) emanate from burn-injured tissue and enter systemic circulation. Locally and systemically, they activate pattern-recognition receptors, including toll-like receptors (TLRs), to stimulate cytokine secretion, wh...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2017.0733
更新日期:2017-10-01 00:00:00
abstract::Significance: Advances in field-based trauma care, surgical techniques, and protective equipment have collectively facilitated the survival of a historically large number of service members (SMs) following combat trauma, although many sustained significant composite tissue injuries to the extremities, including limb l...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2016.0714
更新日期:2017-08-01 00:00:00
abstract::Significance: Pressure ulcers (PUs) in newborns and children are remarkably different from those in adults, both in their possible causal factors and in the etiology and biomechanical pathways for tissue damage. Recent Advances: Pediatric muscle and fat tissue structures are overall softer than those of adults, making...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2015.0639
更新日期:2015-10-01 00:00:00
abstract::Objective: To develop a cost-effective and clinically usable therapy to treat full-thickness skin injuries. We accomplished this by preparing a viscoelastic hydrogel using polyethylene glycol (PEG)-modified platelet-free plasma (PEGylated PFP) combined with human adipose-derived stem cells (ASCs). Approach: PEGylated ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0844
更新日期:2019-07-01 00:00:00
abstract::Objective: The contemporary treatment of a full-thickness burn consists of early eschar excision followed by immediate closure of the open wound using autologous skin. However, most animal models study burn wound healing with the persistence of the burn eschar. Our goal is to characterize a murine model of burn eschar...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2014.0570
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:Over the past decade, the development of high-throughput technologies for DNA and protein analysis has revolutionized the ways in which cells can be studied. Within a relatively short time frame, research has changed from studying individual genes and proteins to analyzing entire genomes and proteomes. APPRO...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2012.0379
更新日期:2013-11-01 00:00:00
abstract::Significance: Chronic skin ulcers, including venous, diabetic, and pressure ulcers, constitute a major health care burden, affecting 2-6 million people in the United States alone, with projected increases in incidence owing to the aging population and rising epidemic of diabetes. The ulcers are often accompanied by pa...
journal_title:Advances in wound care
pub_type: 杂志文章,评审
doi:10.1089/wound.2018.0866
更新日期:2019-04-01 00:00:00
abstract:SIGNIFICANCE:Epidermal growth factor (EGF) and EGF receptor (EGFR) play an essential role in wound healing through stimulating epidermal and dermal regeneration. The development of new therapies for enhancing wound healing has included the use of EGF. In addition, EGFR inhibitors (EGFRis) have become a therapeutic opti...
journal_title:Advances in wound care
pub_type: 评审
doi:10.1089/wound.2011.0326
更新日期:2013-02-01 00:00:00
abstract::Objective: There are no safety or absorption studies to guide topical timolol therapy for treatment of chronic wounds. This study was undertaken to address this gap. Approach: A prospective, observational, cross-sectional comparative study of timolol plasma levels in patients after topical administration to a chronic ...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2019.0970
更新日期:2019-11-01 00:00:00
abstract::Objective: Wound healing is a complex process involving the dynamic interplay of various types of cells and bioactive factors. Impaired wound healing is characterized by a loss in synchronization of the process, resulting in non-healing chronic wounds. Human amniotic membrane (AM) has been shown to be effective in the...
journal_title:Advances in wound care
pub_type: 杂志文章
doi:10.1089/wound.2018.0931
更新日期:2019-08-01 00:00:00