Abstract:
:Heterochiral DNA strand-displacement reactions enable sequence-specific interfacing of oligonucleotide enantiomers, making it possible to interface native d-nucleic acids with molecular circuits built using nuclease-resistant l-DNA. To date, all heterochiral reactions have relied on peptide nucleic acid (PNA), which places potential limits on the scope and utility of this approach. Herein, we now report heterochiral strand-displacement in the absence of PNA, instead utilizing chimeric d/l-DNA complexes to interface oligonucleotides of the opposite chirality. We show that these strand-displacement reactions can be easily integrated into multicomponent heterochiral circuits, are compatible with both DNA and RNA inputs, and can be engineered to function in serum-supplemented medium. We anticipate that these new reactions will lead to a wider application of heterochiral strand-displacement, especially in the design of biocompatible nucleic acid circuits that can reliably operate within living systems.
journal_name
ACS Synth Bioljournal_title
ACS synthetic biologyauthors
Young BE,Sczepanski JTdoi
10.1021/acssynbio.9b00335subject
Has Abstractpub_date
2019-12-20 00:00:00pages
2756-2759issue
12issn
2161-5063journal_volume
8pub_type
杂志文章abstract::This paper describes SBOLExplorer, a system that is used to provide intuitive searching within the SynBioHub genetic design repository. SynBioHub stores genetic constructs encoded in the SBOL data format. These constructs can represent genetic parts, circuits, and sequences. These constructs are often numerous, exist ...
journal_title:ACS synthetic biology
pub_type: 杂志文章
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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doi:10.1021/acssynbio.6b00211
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abstract::Design and implementation of synthetic biological circuits highly depends on well-characterized, robust promoters with predictable input-output responses. While great progress has been made with heterotrophic model organisms such as Escherichia coli, the available variety of tunable promoter parts for phototrophic cya...
journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
pub_type: 杂志文章
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journal_title:ACS synthetic biology
pub_type: 杂志文章
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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doi:10.1021/acssynbio.7b00113
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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doi:10.1021/acssynbio.9b00063
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journal_title:ACS synthetic biology
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