Abstract:
:Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors that rely on broad host range origins for bacterial replication. Here we present pLX vectors, a set of mini binary T-DNA plasmids suitable for Type IIS restriction endonuclease- and overlap-based assembly methods. pLX vectors include replicons from compatible broad host range plasmids. Simultaneous usage of pBBR1- and RK2-based pLX vectors in a two-plasmid/one-Agrobacterium strain strategy allowed multigene delivery to plants. Adoption of pLX vectors will facilitate routine plant transformations and targeted mutagenesis, as well as complex part and circuit characterization.
journal_name
ACS Synth Bioljournal_title
ACS synthetic biologyauthors
Pasin F,Bedoya LC,Bernabé-Orts JM,Gallo A,Simón-Mateo C,Orzaez D,García JAdoi
10.1021/acssynbio.6b00354subject
Has Abstractpub_date
2017-10-20 00:00:00pages
1962-1968issue
10issn
2161-5063journal_volume
6pub_type
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
pub_type: 杂志文章
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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