当前位置： SCI文献检索 > ACS Synthetic Biology期刊下所有文献 > Metabolic Rewiring Improves the Production of the Fungal Active Targeting Molecule Fusarinine C.

Metabolic Rewiring Improves the Production of the Fungal Active Targeting Molecule Fusarinine C.

Abstract：

:Author: Recently, increasing research in siderophores has been dedicated to their possible medical applications in diagnostics and therapeutics for human pathogenic infections. Fusarinine C (FsC) is a natural hydroxamate siderophore that harbors three amino groups, which allow the easy chemical modification of FsC for the design of novel multifunctional conjugates. However, low production of FsC has hampered its extensive exploitation.Herein, we rewired the FsC biosynthetic pathway in the Aureobasidium melanogenum HN6.2 strain to achieve a self-supplying l-ornithine with component-simplified and enhanced production of extracellular siderophores, for which the FsC accounted for 94%, its final titer being approximately 1.7 g L-1. The convenient acquisition of FsC effectuated our exploitation for its application. We employed in vitro and in vivo assays to show that FsC is an active targeting molecule that acts on the human pathogenic fungi Trichophyton rubrum and Candida albicans; this demonstrates the potential to use FsC for the development of novel antifungal targeting reagents in the future.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Lu Y,Wang H,Wang Z,Cong Y,Zhang P,Liu G,Liu C,Chi Z,Chi Z

doi

10.1021/acssynbio.9b00026

subject

Has Abstract

pub_date

2019-08-16 00:00:00

pages

1755-1765

issue

8

issn

2161-5063

journal_volume

8

pub_type

杂志文章

相关文献

ACS Synthetic Biology文献大全
  • Comparative Dose-Response Analysis of Inducible Promoters in Cyanobacteria.

    abstract：:Design and implementation of synthetic biological circuits highly depends on well-characterized, robust promoters with predictable input-output responses. While great progress has been made with heterotrophic model organisms such as Escherichia coli, the available variety of tunable promoter parts for phototrophic cya...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00505

    authors： Behle A,Saake P,Germann AT,Dienst D,Axmann IM

    更新日期：2020-04-17 00:00:00

  • ShortBOL: A Language for Scripting Designs for Engineered Biological Systems Using Synthetic Biology Open Language (SBOL).

    abstract：:The Synthetic Biology Open Language (SBOL) is an emerging synthetic biology data exchange standard, designed primarily for unambiguous and efficient machine-to-machine communication. However, manual editing of SBOL is generally difficult for nontrivial designs. Here, we describe ShortBOL, a lightweight SBOL scripting ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00470

    authors： Crowther M,Grozinger L,Pocock M,Taylor CPD,McLaughlin JA,Mısırlı G,Bartley BA,Beal J,Goñi-Moreno A,Wipat A

    更新日期：2020-04-17 00:00:00

  • Design and Characterization of an Icosahedral Protein Cage Formed by a Double-Fusion Protein Containing Three Distinct Symmetry Elements.

    abstract：:Exploiting simple types of symmetry common to many natural protein oligomers as a starting point, several recent studies have succeeded in engineering complex self-assembling protein architectures reminiscent but distinct from those evolved in the natural world. Designing symmetric protein cages with a wide range of p...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00392

    authors： Cannon KA,Nguyen VN,Morgan C,Yeates TO

    更新日期：2020-03-20 00:00:00

  • Owl: electronic datasheet generator.

    abstract：:Owl ( www.owlcad.org ) is a biodesign automation tool that generates electronic datasheets for synthetic biological parts using common formatting. Data can be retrieved automatically from existing repositories and modified in the Owl user interface (UI). Owl uses the data to generate an HTML page with standard typeset...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb500053j

    authors： Appleton E,Tao J,Wheatley FC,Desai DH,Lozanoski TM,Shah PD,Awtry JA,Jin SS,Haddock TL,Densmore DM

    更新日期：2014-12-19 00:00:00

  • An AND-Gated Drug and Photoactivatable Cre-loxP System for Spatiotemporal Control in Cell-Based Therapeutics.

    abstract：:While engineered chimeric antigen receptor (CAR) T cells have shown promise in detecting and eradicating cancer cells within patients, it remains difficult to identify a set of truly cancer-specific CAR-targeting cell surface antigens to prevent potentially fatal on-target off-tumor toxicity against other healthy tiss...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00175

    authors： Allen ME,Zhou W,Thangaraj J,Kyriakakis P,Wu Y,Huang Z,Ho P,Pan Y,Limsakul P,Xu X,Wang Y

    更新日期：2019-10-18 00:00:00

  • Engineering Prokaryotic Transcriptional Activator XylR as a Xylose-Inducible Biosensor for Transcription Activation in Yeast.

    abstract：:Biosensors regulated by specific substrates are needed to develop genetic tools to meet the needs of engineering microbial cell factories. Here, a xylose-inducible biosensor (xylbiosensor), comprising the Escherichia coli activation factor XylR, fusion activation domain (AD) VPRH, and a hybrid promoter with operator x...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00122

    authors： Wei W,Shang Y,Zhang P,Liu Y,You D,Yin B,Ye B

    更新日期：2020-05-15 00:00:00

  • Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli.

    abstract：:The structural complexity of nonribosomal peptides (NRPs) impeding economic chemical synthesis and poor cultivability of source organisms limits the development of bioprocesses for novel bioactive compounds. Since nonribosomal peptide synthetases (NRPSs) assemble NRPs from simple amino acid building blocks, heterologo...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb400082j

    authors： Jaitzig J,Li J,Süssmuth RD,Neubauer P

    更新日期：2014-07-18 00:00:00

  • An Orthogonal Multi-input Integration System to Control Gene Expression in Escherichia coli.

    abstract：:In many biotechnological applications, it is useful for gene expression to be regulated by multiple signals, as this allows the programming of complex behavior. Here we implement, in Escherichia coli, a system that compares the concentration of two signal molecules, and tunes GFP expression proportionally to their rel...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.7b00109

    authors： Annunziata F,Matyjaszkiewicz A,Fiore G,Grierson CS,Marucci L,di Bernardo M,Savery NJ

    更新日期：2017-10-20 00:00:00

  • Biophysical, mutational, and functional investigation of the chromophore-binding pocket of light-oxygen-voltage photoreceptors.

    abstract：:As light-regulated actuators, sensory photoreceptors underpin optogenetics and numerous applications in synthetic biology. Protein engineering has been applied to fine-tune the properties of photoreceptors and to generate novel actuators. For the blue-light-sensitive light-oxygen-voltage (LOV) photoreceptors, mutation...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb400205x

    authors： Diensthuber RP,Engelhard C,Lemke N,Gleichmann T,Ohlendorf R,Bittl R,Möglich A

    更新日期：2014-11-21 00:00:00

  • Reversing an Extracellular Electron Transfer Pathway for Electrode-Driven Acetoin Reduction.

    abstract：:Microbial electrosynthesis is an emerging technology with the potential to simultaneously store renewably generated energy, fix carbon dioxide, and produce high-value organic compounds. However, limited understanding of the route of electrons into the cell remains an obstacle to developing a robust microbial electrosy...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00498

    authors： Tefft NM,TerAvest MA

    更新日期：2019-07-19 00:00:00

  • Directed acyclic graph-based technology mapping of genetic circuit models.

    abstract：:As engineering foundations such as standards and abstraction begin to mature within synthetic biology, it is vital that genetic design automation (GDA) tools be developed to enable synthetic biologists to automatically select standardized DNA components from a library to meet the behavioral specification for a genetic...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb400135t

    authors： Roehner N,Myers CJ

    更新日期：2014-08-15 00:00:00

  • Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1.

    abstract：:Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb3000244

    authors： Zhang D,Zhao Y,He Y,Wang Y,Zhao Y,Zheng Y,Wei X,Zhang L,Li Y,Jin T,Wu L,Wang H,Davison PA,Xu J,Huang WE

    更新日期：2012-07-20 00:00:00

  • Single Day Construction of Multigene Circuits with 3G Assembly.

    abstract：:The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting step of the prototyping cycle for synthetic biology. Recently developed DNA assembly methods such as isothermal assembly and type IIS restriction enzyme systems t...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00060

    authors： Halleran AD,Swaminathan A,Murray RM

    更新日期：2018-05-18 00:00:00

  • A Novel Approach for Gene Expression Optimization through Native Promoter and 5' UTR Combinations Based on RNA-seq, Ribo-seq, and TSS-seq of Streptomyces coelicolor.

    abstract：:Streptomycetes are Gram-positive mycelial bacteria, which synthesize a wide range of natural products including over two-thirds of the currently available antibiotics. However, metabolic engineering in Streptomyces species to overproduce a vast of natural products are hampered by a limited number of genetic tools. Her...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.6b00263

    authors： Yi JS,Kim MW,Kim M,Jeong Y,Kim EJ,Cho BK,Kim BG

    更新日期：2017-03-17 00:00:00

  • Lessons from Two Design-Build-Test-Learn Cycles of Dodecanol Production in Escherichia coli Aided by Machine Learning.

    abstract：:The Design-Build-Test-Learn (DBTL) cycle, facilitated by exponentially improving capabilities in synthetic biology, is an increasingly adopted metabolic engineering framework that represents a more systematic and efficient approach to strain development than historical efforts in biofuels and biobased products. Here, ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00020

    authors： Opgenorth P,Costello Z,Okada T,Goyal G,Chen Y,Gin J,Benites V,de Raad M,Northen TR,Deng K,Deutsch S,Baidoo EEK,Petzold CJ,Hillson NJ,Garcia Martin H,Beller HR

    更新日期：2019-06-21 00:00:00

  • A System for Gene Expression Noise Control in Yeast.

    abstract：:Gene expression noise arises from stochastic variation in the synthesis and degradation of mRNA and protein molecules and creates differences in protein numbers across populations of genetically identical cells. Such variability can lead to imprecision and reduced performance of both native and synthetic networks. In ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00279

    authors： Mundt M,Anders A,Murray SM,Sourjik V

    更新日期：2018-11-16 00:00:00

  • Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis.

    abstract：:Cell-free protein synthesis (CFPS) is a widely used research tool in systems and synthetic biology. However, if CFPS is to become a mainstream technology for applications such as point of care manufacturing, we must understand the performance limits and costs of these systems. Toward this question, we used sequence sp...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.7b00465

    authors： Vilkhovoy M,Horvath N,Shih CH,Wayman JA,Calhoun K,Swartz J,Varner JD

    更新日期：2018-08-17 00:00:00

  • Semisupervised Gaussian Process for Automated Enzyme Search.

    abstract：:Synthetic biology is today harnessing the design of novel and greener biosynthesis routes for the production of added-value chemicals and natural products. The design of novel pathways often requires a detailed selection of enzyme sequences to import into the chassis at each of the reaction steps. To address such desi...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.5b00294

    authors： Mellor J,Grigoras I,Carbonell P,Faulon JL

    更新日期：2016-06-17 00:00:00

  • Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins.

    abstract：:In this work we report synthetic adhesins (SAs) enabling the rational design of the adhesion properties of E. coli. SAs have a modular structure comprising a stable β-domain for outer membrane anchoring and surface-exposed immunoglobulin domains with high affinity and specificity that can be selected from large repert...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb500252a

    authors： Piñero-Lambea C,Bodelón G,Fernández-Periáñez R,Cuesta AM,Álvarez-Vallina L,Fernández LÁ

    更新日期：2015-04-17 00:00:00

  • Strategies for Editing Virulent Staphylococcal Phages Using CRISPR-Cas10.

    abstract：:Staphylococci are prevalent skin-dwelling bacteria that are also leading causes of antibiotic-resistant infections. Viruses that infect and lyse these organisms (virulent staphylococcal phages) can be used as alternatives to conventional antibiotics and represent promising tools to eliminate or manipulate specific spe...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.7b00240

    authors： Bari SMN,Walker FC,Cater K,Aslan B,Hatoum-Aslan A

    更新日期：2017-12-15 00:00:00

  • Silencing of antibiotic resistance in E. coli with engineered phage bearing small regulatory RNAs.

    abstract：:In response to emergent antibiotic resistance, new strategies are needed to enhance the effectiveness of existing antibiotics. Here, we describe a phagemid-delivered, RNA-mediated system capable of directly knocking down antibiotic resistance phenotypes. Small regulatory RNAs (sRNAs) were designed to specifically inhi...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb500033d

    authors： Libis VK,Bernheim AG,Basier C,Jaramillo-Riveri S,Deyell M,Aghoghogbe I,Atanaskovic I,Bencherif AC,Benony M,Koutsoubelis N,Löchner AC,Marinkovic ZS,Zahra S,Zegman Y,Lindner AB,Wintermute EH

    更新日期：2014-12-19 00:00:00

  • Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli.

    abstract：:Although Escherichia coli has been a popular tool for plasmid construction, this bacterium was believed to be "unsuitable" for constructing a large plasmid whose size exceeds 500 kilobases. We assumed that traditional plasmid vectors may lack some regulatory DNA elements required for the stable replication and segrega...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00008

    authors： Mukai T,Yoneji T,Yamada K,Fujita H,Nara S,Su'etsugu M

    更新日期：2020-06-19 00:00:00

  • Rational Design of the N-Terminal Coding Sequence for Regulating Enzyme Expression in Bacillus subtilis.

    abstract：:Synonymous mutation of the N-terminal coding sequence (NCS) has been used to regulate gene expression. We here developed a statistical model to predict the effect of the NCSs on protein expression in Bacillus subtilis WB600. First, a synonymous mutation was performed within the first 10 residues of a superfolder green...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00309

    authors： Xu K,Tong Y,Li Y,Tao J,Li J,Zhou J,Liu S

    更新日期：2021-01-19 00:00:00

  • Combined Assembly and Targeted Integration of Multigene for Nitrogenase Biosynthetic Pathway in Saccharomyces cerevisiae.

    abstract：:Biological nitrogen fixation, a process unique to diazotrophic prokaryote, is catalyzed by the nitrogenase complex. There has been a long-standing interest in reconstituting a nitrogenase biosynthetic pathway in a eukaryotic host with the final aim of developing N2-fixing cereal crops. In this study, we report that a ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00060

    authors： Liu X,Wang M,Song Y,Li Y,Liu P,Shi H,Li Y,Hao T,Zhang H,Jiang W,Chen S,Li J

    更新日期：2019-08-16 00:00:00

  • Light-Inducible Recombinases for Bacterial Optogenetics.

    abstract：:Optogenetic tools can provide direct and programmable control of gene expression. Light-inducible recombinases, in particular, offer a powerful method for achieving precise spatiotemporal control of DNA modification. However, to-date this technology has been largely limited to eukaryotic systems. Here, we develop opto...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00395

    authors： Sheets MB,Wong WW,Dunlop MJ

    更新日期：2020-02-21 00:00:00

  • 3D Printing for the Fabrication of Biofilm-Based Functional Living Materials.

    abstract：:Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00192

    authors： Balasubramanian S,Aubin-Tam ME,Meyer AS

    更新日期：2019-07-19 00:00:00

  • Development of an artificial calcium-dependent transcription factor to detect sustained intracellular calcium elevation.

    abstract：:The development of a synthetic transcription factor that responds to intracellular calcium signals enables analyzing cellular events at the single-cell level or "rewiring" the intracellular information networks. In this study, we developed the calcium-dependent transcription factor (CaTF), which was cleaved by calpain...

    journal_title：ACS synthetic biology

    pub_type： 信件

    doi：10.1021/sb500070c

    authors： Suzuki S,Murotomi K,Nakajima Y,Kawai K,Ohta K,Warita K,Miki T,Takeuchi Y

    更新日期：2014-10-17 00:00:00

  • Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins.

    abstract：:Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors that rely on broad host range origins for bacterial replication. Here we present pLX vect...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.6b00354

    authors： Pasin F,Bedoya LC,Bernabé-Orts JM,Gallo A,Simón-Mateo C,Orzaez D,García JA

    更新日期：2017-10-20 00:00:00

  • Exploring Catalysis Specificity of Phytoene Dehydrogenase CrtI in Carotenoid Synthesis.

    abstract：:Carotenoids, a variety of natural products, have significant pharmaceutical and commercial potential. Phytoene dehydrogenase (CrtI) is the rate-limit enzyme for carotenoid synthesis, whose catalysis specificity results in various carotenoids. However, the structural characteristics of CrtI for controlling the catalysi...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00128

    authors： Liang N,Chen C,Wang Y,Ding MZ,Yao MD,Xiao WH,Yuan YJ

    更新日期：2020-07-17 00:00:00

  • Cloning, Stability, and Modification of Mycoplasma hominis Genome in Yeast.

    abstract：:Mycoplasma hominis is a minimal human pathogen that is responsible for genital and neonatal infections. Despite many attempts, there is no efficient genetic tool to manipulate this bacterium, limiting most investigations of its pathogenicity and its uncommon energy metabolism that relies on arginine. The recent clonin...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.6b00379

    authors： Rideau F,Le Roy C,Descamps ECT,Renaudin H,Lartigue C,Bébéar C

    更新日期：2017-05-19 00:00:00