Abstract:
:Owl ( www.owlcad.org ) is a biodesign automation tool that generates electronic datasheets for synthetic biological parts using common formatting. Data can be retrieved automatically from existing repositories and modified in the Owl user interface (UI). Owl uses the data to generate an HTML page with standard typesetting that can be saved as a PDF file. Here we present the Owl software tool in its alpha version, its current UI, its description of input data for generating a datasheet, its example datasheets, and the vision of the tool's role in biodesign automation.
journal_name
ACS Synth Bioljournal_title
ACS synthetic biologyauthors
Appleton E,Tao J,Wheatley FC,Desai DH,Lozanoski TM,Shah PD,Awtry JA,Jin SS,Haddock TL,Densmore DMdoi
10.1021/sb500053jsubject
Has Abstractpub_date
2014-12-19 00:00:00pages
966-8issue
12issn
2161-5063journal_volume
3pub_type
杂志文章abstract::Cell-free protein synthesis (CFPS) is a widely used research tool in systems and synthetic biology. However, if CFPS is to become a mainstream technology for applications such as point of care manufacturing, we must understand the performance limits and costs of these systems. Toward this question, we used sequence sp...
journal_title:ACS synthetic biology
pub_type: 杂志文章
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更新日期:2018-08-17 00:00:00
abstract::Light-switchable proteins enable unparalleled control of molecular biological processes in live organisms. Previously, we have engineered red/far-red and green/red photoreversible two-component signal transduction systems (TCSs) with transcriptional outputs in E. coli and used them to characterize and control syntheti...
journal_title:ACS synthetic biology
pub_type: 杂志文章
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abstract::To elucidate the function of mevalonate-5-pyrophosphate decarboxylase (MVD) and farnesyl pyrophosphate synthase (FPS) in triterpene biosynthesis, the genes governing the expression of these enzymes were transformed into Panax ginseng hairy roots. All the transgenic lines showed higher expression levels of PgMVD and Pg...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/sb400194g
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.6b00189
更新日期:2017-02-17 00:00:00
abstract::Streptomycetes are Gram-positive mycelial bacteria, which synthesize a wide range of natural products including over two-thirds of the currently available antibiotics. However, metabolic engineering in Streptomyces species to overproduce a vast of natural products are hampered by a limited number of genetic tools. Her...
journal_title:ACS synthetic biology
pub_type: 杂志文章
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.8b00041
更新日期:2018-07-20 00:00:00
abstract::Simple computation can be performed using the interactions between single-stranded molecules of DNA. These interactions are typically toehold-mediated strand displacement reactions in a well-mixed solution. We demonstrate that a DNA circuit with tethered reactants is a distributed system and show how it can be describ...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.5b00275
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abstract::Movement of molecules across membranes in response to a stimulus is a key component of cellular programming. Here, we characterize and manipulate the response of a protein-based piston capable of puncturing membranes in a pH-dependent manner. Our protein actuator consists of modified R bodies found in a bacterial endo...
journal_title:ACS synthetic biology
pub_type: 信件
doi:10.1021/acssynbio.5b00237
更新日期:2016-04-15 00:00:00
abstract::Dissimilatory metal-reducing bacteria, particularly those from the genus Shewanella, are of importance for bioremediation of metal contaminated sites and sustainable energy production. However, studies on this species have suffered from a lack of effective genetic tools for precise and high throughput genome manipulat...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.9b00188
更新日期:2019-08-16 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.7b00240
更新日期:2017-12-15 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/sb400016j
更新日期:2013-12-20 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.6b00166
更新日期:2017-02-17 00:00:00
abstract::We study translation repression in bacteria by engineering a regulatory circuit that functions as a binding assay for RNA binding proteins (RBP) in vivo. We do so by inducing expression of a fluorescent protein-RBP chimera, together with encoding its binding site at various positions within the ribosomal initiation re...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.8b00378
更新日期:2018-12-21 00:00:00
abstract::Lactams are an important class of commodity chemicals used in the manufacture of nylons, with millions of tons produced every year. Biological production of lactams could be greatly improved by high-throughput sensors for lactam biosynthesis. To identify biosensors of lactams, we applied a chemoinformatic approach ins...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.6b00136
更新日期:2017-03-17 00:00:00
abstract::Living organisms employ endogenous negative feedback loops to maintain homeostasis despite environmental fluctuations. A pressing open challenge in Synthetic Biology is to design and implement synthetic circuits to control host cells' behavior, in order to regulate and maintain desired conditions. To cope with the hig...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.6b00220
更新日期:2017-03-17 00:00:00
abstract::In response to emergent antibiotic resistance, new strategies are needed to enhance the effectiveness of existing antibiotics. Here, we describe a phagemid-delivered, RNA-mediated system capable of directly knocking down antibiotic resistance phenotypes. Small regulatory RNAs (sRNAs) were designed to specifically inhi...
journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/sb500033d
更新日期:2014-12-19 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.6b00261
更新日期:2017-04-21 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.7b00113
更新日期:2017-09-15 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.6b00150
更新日期:2016-12-16 00:00:00
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journal_title:ACS synthetic biology
pub_type: 杂志文章
doi:10.1021/acssynbio.0c00415
更新日期:2020-12-18 00:00:00
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doi:10.1021/acssynbio.0c00497
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
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journal_title:ACS synthetic biology
pub_type: 信件
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journal_title:ACS synthetic biology
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pub_type: 杂志文章
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