3D Printing for the Fabrication of Biofilm-Based Functional Living Materials.


:Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to degradation of toxic chemicals. Biofilms exhibit high mechanical stiffness and are inherently tolerant to adverse conditions including the presence of antibiotics, pollutants, detergents, high temperature, changes in pH, etc. These features make biofilms resilient, which is beneficial for applications in dynamic environments such as bioleaching, bioremediation, materials production, and wastewater purification. We have recently described an easy and cost-effective method for 3D printing of bacteria and have extended this technology for 3D printing of genetically engineered Escherichia coli biofilms. Our 3D printing platform exploits simple alginate chemistry for printing of a bacteria-alginate bioink mixture onto calcium-containing agar surfaces, resulting in the formation of bacteria-encapsulating hydrogels with varying geometries. Bacteria in these hydrogels remain intact, spatially patterned, and viable for several days. Printing of engineered bacteria to produce inducible biofilms leads to formation of multilayered three-dimensional structures that can tolerate harsh chemical treatments. Synthetic biology and material science approaches provide the opportunity to append a wide range of useful functionalities to these 3D-printed biofilms. In this article, we describe the wide range of future applications possible for applying functional 3D-printed biofilms to the construction of living biofilm-derived materials in a large-scale and environmentally stable manner.


ACS Synth Biol


ACS synthetic biology


Balasubramanian S,Aubin-Tam ME,Meyer AS




Has Abstract


2019-07-19 00:00:00










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  • Exploring Chemical Biosynthetic Design Space with Transform-MinER.

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    authors: Mundt M,Anders A,Murray SM,Sourjik V

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  • Exploring the heterologous genomic space for building, stepwise, complex, multicomponent tolerance to toxic chemicals.

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    authors: Zingaro KA,Nicolaou SA,Yuan Y,Papoutsakis ET

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  • The Formal Language and Design Principles of Autonomous DNA Walker Circuits.

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    authors: Chomvong K,Lin E,Blaisse M,Gillespie AE,Cate JH

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


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  • Directed acyclic graph-based technology mapping of genetic circuit models.

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Roehner N,Myers CJ

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  • Analog Computation by DNA Strand Displacement Circuits.

    abstract::DNA circuits have been widely used to develop biological computing devices because of their high programmability and versatility. Here, we propose an architecture for the systematic construction of DNA circuits for analog computation based on DNA strand displacement. The elementary gates in our architecture include ad...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Song T,Garg S,Mokhtar R,Bui H,Reif J

    更新日期:2016-08-19 00:00:00

  • RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids.

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Horning DP,Bala S,Chaput JC,Joyce GF

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Durante-Rodríguez G,de Lorenzo V,Nikel PI

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    abstract::Light-switchable proteins enable unparalleled control of molecular biological processes in live organisms. Previously, we have engineered red/far-red and green/red photoreversible two-component signal transduction systems (TCSs) with transcriptional outputs in E. coli and used them to characterize and control syntheti...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Schmidl SR,Sheth RU,Wu A,Tabor JJ

    更新日期:2014-11-21 00:00:00

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Li T,Ye J,Shen R,Zong Y,Zhao X,Lou C,Chen GQ

    更新日期:2016-11-18 00:00:00

  • A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis.

    abstract::Eukaryotic transcriptional factors (TFs) typically recognize short genomic sequences alone or together with other proteins to modulate gene expression. Mapping of TF-DNA interactions in the genome is crucial for understanding the gene regulatory programs in cells. While chromatin immunoprecipitation followed by sequen...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Xiong X,Zhang Y,Yan J,Jain S,Chee S,Ren B,Zhao H

    更新日期:2017-06-16 00:00:00