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Cellular signaling circuits interfaced with synthetic, post-translational, negating Boolean logic devices.

Abstract：

:A negating functionality is fundamental to information processing of logic circuits within cells and computers. Aiming to adapt unutilized electronic concepts to the interrogation of signaling circuits in cells, we first took a bottom-up strategy whereby we created protein-based devices that perform negating Boolean logic operations such as NOT, NOR, NAND, and N-IMPLY. These devices function in living cells within a minute by precisely commanding the localization of an activator molecule among three subcellular spaces. We networked these synthetic gates to an endogenous signaling circuit and devised a physiological output. In search of logic functions in signal transduction, we next took a top-down approach and computationally screened 108 signaling pathways to identify commonalities and differences between these biological pathways and electronic circuits. This combination of synthetic and systems approaches will guide us in developing foundations for deconstruction of intricate cell signaling, as well as construction of biomolecular computers.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Razavi S,Su S,Inoue T

doi

10.1021/sb500222z

subject

Has Abstract

pub_date

2014-09-19 00:00:00

pages

676-85

issue

9

issn

2161-5063

journal_volume

3

pub_type

杂志文章

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