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Homologous Quorum Sensing Regulatory Circuit: A Dual-Input Genetic Controller for Modulating Quorum Sensing-Mediated Protein Expression in E. coli.

Abstract：

:We developed a hybrid synthetic circuit that co-opts the genetic regulation of the native bacterial quorum sensing autoinducer-2 and imposes an extra external controller for maintaining tightly controlled gene expression. This dual-input genetic controller was mathematically modeled and, by design, can be operated in three modes: a constitutive mode that enables consistent and high levels of expression; a tightly repressed mode in which there is very little background expression; and an inducible mode in which concentrations of two signals (arabinose and autoinducer-2) determine the net amplification of the gene(s)-of-interest. We demonstrate the utility of the circuit for the controlled expression of human granulocyte macrophage colony stimulating factor in an engineered probiotic E. coli. This dual-input genetic controller is the first homologous AI-2 quorum sensing circuit that has the ability to be operated in three different modes. We believe it has the potential for wide-ranging biotechnological applications due its versatile features.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Hauk P,Stephens K,Virgile C,VanArsdale E,Pottash AE,Schardt JS,Jay SM,Sintim HO,Bentley WE

doi

10.1021/acssynbio.0c00179

subject

Has Abstract

pub_date

2020-10-16 00:00:00

pages

2692-2702

issue

10

issn

2161-5063

journal_volume

9

pub_type

杂志文章

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