Toward the First Data Acquisition Standard in Synthetic Biology.

abstract：:Fluorescent proteins are widely used as fusion tags to detect protein expression in vivo. To become fluorescent, these proteins must undergo chromophore maturation, a slow process with a half-time of 5 to >30 min that causes delays in real-time detection of protein expression. Here, we engineer a genetically encoded f...

journal_title：ACS synthetic biology

authors： Eason MG,Pandelieva AT,Mayer MM,Khan ST,Garcia HG,Chica RA

更新日期：2020-11-20 00:00:00

abstract：:Directed evolution requires the creation of genetic diversity and subsequent screening or selection for improved variants. For DNA mutagenesis, conventional site-directed methods implicitly utilize the Boolean AND operator (creating all mutations simultaneously), producing a combinatorial explosion in the number of ge...

journal_title：ACS synthetic biology

authors： Currin A,Kwok J,Sadler JC,Bell EL,Swainston N,Ababi M,Day P,Turner NJ,Kell DB

更新日期：2019-06-21 00:00:00

abstract：:The versatility of Ca2+ signals allows it to regulate diverse cellular processes such as migration, apoptosis, motility and exocytosis. In some receptors (e.g., VEGFR2), Ca2+ signals are generated upon binding their ligand(s) (e.g., VEGF-A). Here, we employed a design strategy to engineer proteins that generate a Ca2+...

journal_title：ACS synthetic biology

authors： Qudrat A,Truong K

更新日期：2017-03-17 00:00:00

abstract：:Transform-MinER (Transforming Molecules in Enzyme Reactions) is a web application facilitating the exploration of chemical biosynthetic space, guiding the user toward promising start points for enzyme design projects or directed evolution experiments. Two types of search are possible: Molecule Search allows a user to ...

journal_title：ACS synthetic biology

authors： Tyzack JD,Ribeiro AJM,Borkakoti N,Thornton JM

更新日期：2019-11-15 00:00:00

abstract：:Nanostructures formed by self-assembled peptides have been increasingly exploited as functional materials for a wide variety of applications, from biotechnology to energy. However, it is sometimes challenging to assemble free short peptides into functional supramolecular structures, since not all peptides have the abi...

journal_title：ACS synthetic biology

authors： Abdali Z,Aminzare M,Zhu X,DeBenedictis E,Xie O,Keten S,Dorval Courchesne NM

更新日期：2020-12-18 00:00:00

abstract：:Bacterial microcompartments are subcellular compartments found in many prokaryotes; they consist of a protein shell that encapsulates enzymes that perform a variety of functions. The shell protects the cell from potentially toxic intermediates and colocalizes enzymes for higher efficiency. Accordingly, it is of consid...

journal_title：ACS synthetic biology

authors： Sutter M,McGuire S,Ferlez B,Kerfeld CA

更新日期：2019-04-19 00:00:00

abstract：:The deeply red-colored natural compound prodigiosin is a representative of the prodiginine alkaloid family, which possesses bioactivities as antimicrobial, antitumor, and antimalarial agents. Various bacteria including the opportunistic human pathogen Serratia marcescens and different members of the Streptomycetaceae ...

journal_title：ACS synthetic biology

authors： Klein AS,Domröse A,Bongen P,Brass HUC,Classen T,Loeschcke A,Drepper T,Laraia L,Sievers S,Jaeger KE,Pietruszka J

更新日期：2017-09-15 00:00:00

abstract：:Top-down reduction of the bacterial genome to construct desired chassis cells is important for synthetic biology. However, the current progress in the field of genome reduction is greatly hindered by indispensable life-essential genes that are interspersed throughout the chromosomal loci. Here, we described a new meth...

journal_title：ACS synthetic biology

authors： Xue X,Wang T,Jiang P,Shao Y,Zhou M,Zhong L,Wu R,Zhou J,Xia H,Zhao G,Qin Z

更新日期：2015-06-19 00:00:00

abstract：:Synonymous mutation of the N-terminal coding sequence (NCS) has been used to regulate gene expression. We here developed a statistical model to predict the effect of the NCSs on protein expression in Bacillus subtilis WB600. First, a synonymous mutation was performed within the first 10 residues of a superfolder green...

journal_title：ACS synthetic biology

authors： Xu K,Tong Y,Li Y,Tao J,Li J,Zhou J,Liu S

更新日期：2021-01-19 00:00:00

abstract：:Erythromycin is necessary in medical treatment and known to be biosynthesized with propionyl-CoA as direct precursor. Oversupply of propionyl-CoA induced hyperpropionylation, which was demonstrated as harmful for erythromycin synthesis in Saccharopolyspora erythraea. Herein, we identified three propionyl-CoA synthetas...

journal_title：ACS synthetic biology

authors： You D,Wang MM,Yin BC,Ye BC

更新日期：2019-02-15 00:00:00

abstract：:DNA replication is one of the central functions of the cell. The complexity of modern DNA replication systems raises a question: is it possible to achieve a simpler continuous isothermal DNA replication using fewer proteins? Here, we searched such replication using an evolutionary approach. Through a long-term serial ...

journal_title：ACS synthetic biology

authors： Okauchi H,Sakatani Y,Otsuka K,Ichihashi N

更新日期：2020-07-17 00:00:00

abstract：:While engineered chimeric antigen receptor (CAR) T cells have shown promise in detecting and eradicating cancer cells within patients, it remains difficult to identify a set of truly cancer-specific CAR-targeting cell surface antigens to prevent potentially fatal on-target off-tumor toxicity against other healthy tiss...

journal_title：ACS synthetic biology

authors： Allen ME,Zhou W,Thangaraj J,Kyriakakis P,Wu Y,Huang Z,Ho P,Pan Y,Limsakul P,Xu X,Wang Y

更新日期：2019-10-18 00:00:00

abstract：:Most of the current methods for controlling the formation rate of a key protein or enzyme in cell factories rely on the manipulation of target genes within the pathway. In this article, we present a novel synthetic system for post-translational regulation of protein levels, FENIX, which provides both independent contr...

journal_title：ACS synthetic biology

authors： Durante-Rodríguez G,de Lorenzo V,Nikel PI

更新日期：2018-11-16 00:00:00

abstract：:Noncanonical amino acids form a highly diverse pool of building blocks that can render unique physicochemical properties to peptides and proteins. Here, four methionine analogues with unsaturated and varying side chain lengths were successfully incorporated at four different positions in nisin in Lactococcus lactis th...

journal_title：ACS synthetic biology

authors： Deng J,Viel JH,Chen J,Kuipers OP

更新日期：2020-09-18 00:00:00

abstract：:The availability of different host chassis will greatly expand the range of applications in synthetic biology. Members of the Acetobacteraceae family of Gram-negative bacteria form an attractive class of nonmodel microorganisms that can be exploited to produce industrial chemicals, food and beverage, and biomaterials....

journal_title：ACS synthetic biology

authors： Teh MY,Ooi KH,Danny Teo SX,Bin Mansoor ME,Shaun Lim WZ,Tan MH

更新日期：2019-04-19 00:00:00

abstract：:Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which ...

journal_title：ACS synthetic biology

authors： Moore R,Chandrahas A,Bleris L

更新日期：2014-10-17 00:00:00

abstract：:While predictable design of a genetic circuit's output is a major goal of synthetic biology, it remains a significant challenge because DNA binding sites in the cell affect the concentration of available transcription factors (TF). To mitigate this problem, we propose to use a TF that results from the (reversible) pho...

journal_title：ACS synthetic biology

authors： Nilgiriwala KS,Jiménez J,Rivera PM,Del Vecchio D

更新日期：2015-05-15 00:00:00

abstract：:Biosensors regulated by specific substrates are needed to develop genetic tools to meet the needs of engineering microbial cell factories. Here, a xylose-inducible biosensor (xylbiosensor), comprising the Escherichia coli activation factor XylR, fusion activation domain (AD) VPRH, and a hybrid promoter with operator x...

journal_title：ACS synthetic biology

authors： Wei W,Shang Y,Zhang P,Liu Y,You D,Yin B,Ye B

更新日期：2020-05-15 00:00:00

abstract：:The microbial ability to resist stressful environmental conditions and chemical inhibitors is of great industrial and medical interest. Much of the data related to mutation-based stress resistance, however, is scattered through the academic literature, making it difficult to apply systematic analyses to this wealth of...

journal_title：ACS synthetic biology

authors： Winkler JD,Halweg-Edwards AL,Erickson KE,Choudhury A,Pines G,Gill RT

更新日期：2016-12-16 00:00:00

abstract：:Feedback allows biological systems to control gene expression precisely and reliably, even in the presence of uncertainty, by sensing and processing environmental changes. Taking inspiration from natural architectures, synthetic biologists have engineered feedback loops to tune the dynamics and improve the robustness ...

journal_title：ACS synthetic biology

authors： Agrawal DK,Tang X,Westbrook A,Marshall R,Maxwell CS,Lucks J,Noireaux V,Beisel CL,Dunlop MJ,Franco E

更新日期：2018-05-18 00:00:00

abstract：:In vitro transcription and translation reactions have become popular for a bottom-up approach to synthetic biology. Concentrations of the mRNA intermediate are rarely determined, although knowledge of synthesis and degradation rates could facilitate rational engineering of in vitro systems. We designed binary probes t...

journal_title：ACS synthetic biology

authors： Niederholtmeyer H,Xu L,Maerkl SJ

更新日期：2013-08-16 00:00:00

abstract：:Mycoplasma hominis is a minimal human pathogen that is responsible for genital and neonatal infections. Despite many attempts, there is no efficient genetic tool to manipulate this bacterium, limiting most investigations of its pathogenicity and its uncommon energy metabolism that relies on arginine. The recent clonin...

journal_title：ACS synthetic biology

authors： Rideau F,Le Roy C,Descamps ECT,Renaudin H,Lartigue C,Bébéar C

更新日期：2017-05-19 00:00:00

abstract：:For infectious diseases, rapid and accurate identification of the pathogen is critical for effective management and treatment, but diagnosis remains challenging, particularly in resource-limited areas. Methods that accurately detect pathogen nucleic acids can provide robust, accurate, rapid, and ultrasensitive technol...

journal_title：ACS synthetic biology

authors： Aman R,Mahas A,Mahfouz M

更新日期：2020-06-19 00:00:00

abstract：:Cell-free systems, as part of the synthetic biology field, have become a critical platform in biological studies. However, there is a lack of research into developing a switch for a dynamical control of the transcriptional and translational process. The optogenetic tool has been widely proven as an ideal control switc...

journal_title：ACS synthetic biology

authors： Zhang P,Yang J,Cho E,Lu Y

更新日期：2020-08-21 00:00:00

abstract：:Crossbreeding is a classical yeast hybridization procedure, where the mating of haploid cells of opposite mating-type, MATa and MATα cells, produces a new heterozygous diploid. Here, we describe a method to generate haploid MATa and MATα cells using mating-type conversion caused by expression of the HO gene, which enc...

journal_title：ACS synthetic biology

authors： Fukuda N,Matsukura S,Honda S

更新日期：2013-12-20 00:00:00

abstract：:The cell contents are encapsulated within a compartment, the volume of which is a fundamental physical parameter that may affect intracompartmental reactions. However, there have been few studies to elucidate whether and how volume changes alone can affect the reaction kinetics. It is difficult to address these questi...

journal_title：ACS synthetic biology

authors： Matsuura T,Hosoda K,Kazuta Y,Ichihashi N,Suzuki H,Yomo T

更新日期：2012-09-21 00:00:00

abstract：:Microbial mutualisms play critical roles in a diverse number of ecosystems and have the potential to improve the efficiency of bioproduction for desirable chemicals. We investigate the growth of a photosynthetic cyanobacterium, Synechococcus elongatus PCC 7942, and a diazotroph, Azotobacter vinelandii, in coculture. F...

journal_title：ACS synthetic biology

authors： Smith MJ,Francis MB

更新日期：2016-09-16 00:00:00

abstract：:Sporulated cells have potential as time-delayed expression chassis of proteins for applications such as "on-demand" biologics production, whole cell biosensors, or oral vaccines. However, the desired attributes of high expression rates and low product variances are difficult to maintain from germinated spores. In this...

journal_title：ACS synthetic biology

authors： Tamiev D,Lantz A,Vezeau G,Salis H,Reuel NF

更新日期：2019-10-18 00:00:00

abstract：:Prenylated aromatic compounds are important intermediates in the biosynthesis of bioactive molecules such as 3-chromanols from plants, ubiquinones from prokaryotes and meroterpenoids from sponges. Biosynthesis of prenylated aromatic compounds using prokaryotic microorganisms has attracted increasing attention in the f...

journal_title：ACS synthetic biology

authors： He BB,Bu XL,Zhou T,Li SM,Xu MJ,Xu J

更新日期：2018-09-21 00:00:00

abstract：:Microbial electrosynthesis is an emerging technology with the potential to simultaneously store renewably generated energy, fix carbon dioxide, and produce high-value organic compounds. However, limited understanding of the route of electrons into the cell remains an obstacle to developing a robust microbial electrosy...

journal_title：ACS synthetic biology

authors： Tefft NM,TerAvest MA

更新日期：2019-07-19 00:00:00