Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies.

abstract：:Feedback control allows cells to dynamically sense and respond to environmental changes. However, synthetic controller designs can be challenging because of implementation issues, such as determining optimal expression levels for circuit components within a feedback loop. Here, we addressed this by coupling rational d...

journal_title：ACS synthetic biology

authors： Siu Y,Fenno J,Lindle JM,Dunlop MJ

更新日期：2018-01-19 00:00:00

abstract：:Streptomycetes are Gram-positive mycelial bacteria, which synthesize a wide range of natural products including over two-thirds of the currently available antibiotics. However, metabolic engineering in Streptomyces species to overproduce a vast of natural products are hampered by a limited number of genetic tools. Her...

journal_title：ACS synthetic biology

authors： Yi JS,Kim MW,Kim M,Jeong Y,Kim EJ,Cho BK,Kim BG

更新日期：2017-03-17 00:00:00

abstract：:A significant bottleneck in synthetic biology involves screening large genetically encoded libraries for desirable phenotypes such as chemical production. However, transcription factor-based biosensors can be leveraged to screen thousands of genetic designs for optimal chemical production in engineered microbes. In th...

journal_title：ACS synthetic biology

authors： Thompson MG,Costello Z,Hummel NFC,Cruz-Morales P,Blake-Hedges JM,Krishna RN,Skyrud W,Pearson AN,Incha MR,Shih PM,Garcia-Martin H,Keasling JD

更新日期：2019-10-18 00:00:00

abstract：:The design of synthetic cells requires a detailed understanding of the relevance of genes and gene networks underlying complex cellular phenotypes. Transposon-sequencing (Tn-seq) and constraint-based metabolic modeling can be used to probe the core genetic and metabolic networks underlying a biological process. Integr...

journal_title：ACS synthetic biology

authors： diCenzo GC,Mengoni A,Fondi M

更新日期：2019-01-18 00:00:00

abstract：:Design and implementation of synthetic biological circuits highly depends on well-characterized, robust promoters with predictable input-output responses. While great progress has been made with heterotrophic model organisms such as Escherichia coli, the available variety of tunable promoter parts for phototrophic cya...

journal_title：ACS synthetic biology

authors： Behle A,Saake P,Germann AT,Dienst D,Axmann IM

更新日期：2020-04-17 00:00:00

abstract：:In many biotechnological applications, it is useful for gene expression to be regulated by multiple signals, as this allows the programming of complex behavior. Here we implement, in Escherichia coli, a system that compares the concentration of two signal molecules, and tunes GFP expression proportionally to their rel...

journal_title：ACS synthetic biology

authors： Annunziata F,Matyjaszkiewicz A,Fiore G,Grierson CS,Marucci L,di Bernardo M,Savery NJ

更新日期：2017-10-20 00:00:00

abstract：:The availability of different host chassis will greatly expand the range of applications in synthetic biology. Members of the Acetobacteraceae family of Gram-negative bacteria form an attractive class of nonmodel microorganisms that can be exploited to produce industrial chemicals, food and beverage, and biomaterials....

journal_title：ACS synthetic biology

authors： Teh MY,Ooi KH,Danny Teo SX,Bin Mansoor ME,Shaun Lim WZ,Tan MH

更新日期：2019-04-19 00:00:00

abstract：:Many Gram-negative pathogens encode type 3 secretion systems, sophisticated nanomachines that deliver proteins directly into the cytoplasm of mammalian cells. These systems present attractive opportunities for therapeutic protein delivery applications; however, their utility has been limited by their inherent pathogen...

journal_title：ACS synthetic biology

authors： Reeves AZ,Spears WE,Du J,Tan KY,Wagers AJ,Lesser CF

更新日期：2015-05-15 00:00:00

abstract：:Glycolate is an important α-hydroxy acid with a wide range of industrial applications. The current industrial production of glycolate mainly depends on chemical synthesis, but biochemical production from renewable resources using engineered microorganisms is increasingly viewed as an attractive alternative. Crude glyc...

journal_title：ACS synthetic biology

authors： Zhan T,Chen Q,Zhang C,Bi C,Zhang X

更新日期：2020-09-18 00:00:00

abstract：:Prenylated aromatic compounds are important intermediates in the biosynthesis of bioactive molecules such as 3-chromanols from plants, ubiquinones from prokaryotes and meroterpenoids from sponges. Biosynthesis of prenylated aromatic compounds using prokaryotic microorganisms has attracted increasing attention in the f...

journal_title：ACS synthetic biology

authors： He BB,Bu XL,Zhou T,Li SM,Xu MJ,Xu J

更新日期：2018-09-21 00:00:00

abstract：:The measurement of gene expression using fluorescence markers has been a cornerstone of synthetic biology for the past two decades. However, the use of arbitrary units has limited the usefulness of these data for many quantitative purposes. Calibration of fluorescence measurements from flow cytometry and plate reader ...

journal_title：ACS synthetic biology

authors： Fedorec AJH,Robinson CM,Wen KY,Barnes CP

更新日期：2020-09-18 00:00:00

abstract：:Understanding the molecular mechanisms underlying antibiotic resistance requires concerted efforts in enzymology and medicinal chemistry. Here we describe a new synthetic biology approach to antibiotic development, where the presence of tetracycline antibiotics is linked to a life-death selection in Saccharomyces cere...

journal_title：ACS synthetic biology

authors： Scott LH,Mathews JC,Flematti GR,Filipovska A,Rackham O

更新日期：2018-08-17 00:00:00

abstract：:Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to...

journal_title：ACS synthetic biology

authors： Balasubramanian S,Aubin-Tam ME,Meyer AS

更新日期：2019-07-19 00:00:00

abstract：:The cellular response to hypoxia is orchestrated by HIF-1, a heterodimeric transcription factor composed of an α and a β subunit that enables cell survival under low oxygen conditions by altering the transcription of over 300 genes. There is significant evidence that inhibition of HIF-1 would be beneficial for cancer ...

journal_title：ACS synthetic biology

authors： Mistry IN,Tavassoli A

更新日期：2017-03-17 00:00:00

abstract：:One limitation of gene-directed enzyme prodrug therapy (GDEPT) is the difficulty in selectively and efficiently transducing cancer cells with the gene encoding a prodrug-converting enzyme. To circumvent this issue, we sought to move the selectivity from the gene delivery level to the protein level. We developed fusion...

journal_title：ACS synthetic biology

authors： Warren TD,Patel K,Eshleman JR,Ostermeier M

更新日期：2019-05-17 00:00:00

abstract：:Agricultural soils are often cocontaminated with multiple pesticides. Unfortunately, microorganisms isolated from natural environments do not possess the ability to simultaneously degrade different classes of pesticides. Currently, we can use the approaches of synthetic biology to create a strain endowed with various ...

journal_title：ACS synthetic biology

authors： Gong T,Liu R,Zuo Z,Che Y,Yu H,Song C,Yang C

更新日期：2016-05-20 00:00:00

abstract：:Synthetic biology is today harnessing the design of novel and greener biosynthesis routes for the production of added-value chemicals and natural products. The design of novel pathways often requires a detailed selection of enzyme sequences to import into the chassis at each of the reaction steps. To address such desi...

journal_title：ACS synthetic biology

authors： Mellor J,Grigoras I,Carbonell P,Faulon JL

更新日期：2016-06-17 00:00:00

abstract：:Multidrug resistance (MDR) is a globally relevant problem that requires novel approaches. Two-component systems are a promising, yet untapped target for novel antibacterials. They are prevalent in bacteria and absent in mammals, and their activity can be modulated upon perception of various stimuli. Screening pre-exis...

journal_title：ACS synthetic biology

authors： Lehning CE,Heidelberger JB,Reinhard J,Nørholm MHH,Draheim RR

更新日期：2017-07-21 00:00:00

abstract：:There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples and cells, one can both electronically obtain a redox "portrait" of a biological system and, conversely, program gene expression. Here, we have created a cell-based synth...

journal_title：ACS synthetic biology

authors： VanArsdale E,Hörnström D,Sjöberg G,Järbur I,Pitzer J,Payne GF,van Maris AJA,Bentley WE

更新日期：2020-05-15 00:00:00

abstract：:We recently developed a technique for rapidly and reversibly inhibiting protein function through light-inducible sequestration of proteins away from their normal sites of action. Here, we adapt this method for inducible inactivation of Bem1, a scaffold protein involved in budding yeast polarity. We find that acute inh...

journal_title：ACS synthetic biology

authors： Jost AP,Weiner OD

更新日期：2015-10-16 00:00:00

abstract：:Amyloids are highly ordered, hierarchal protein nanoassemblies. Functional amyloids in bacterial biofilms, such as Escherichia coli curli fibers, are formed by the polymerization of monomeric proteins secreted into the extracellular space. Curli is synthesized by living cells, is primarily composed of the major curlin...

journal_title：ACS synthetic biology

authors： Seker UO,Chen AY,Citorik RJ,Lu TK

更新日期：2017-02-17 00:00:00

abstract：:Biological nitrogen fixation, a process unique to diazotrophic prokaryote, is catalyzed by the nitrogenase complex. There has been a long-standing interest in reconstituting a nitrogenase biosynthetic pathway in a eukaryotic host with the final aim of developing N2-fixing cereal crops. In this study, we report that a ...

journal_title：ACS synthetic biology

authors： Liu X,Wang M,Song Y,Li Y,Liu P,Shi H,Li Y,Hao T,Zhang H,Jiang W,Chen S,Li J

更新日期：2019-08-16 00:00:00

abstract：:Increasing protein expression levels is a key step in the commercial production of enzymes. Predicting promoter activity and translation initiation efficiency based solely on consensus sequences have so far met with mixed results. Here, we addressed this challenge using a "brute-force" approach by designing and synthe...

journal_title：ACS synthetic biology

authors： Sauer C,Ver Loren van Themaat E,Boender LGM,Groothuis D,Cruz R,Hamoen LW,Harwood CR,van Rij T

更新日期：2018-07-20 00:00:00

abstract：:This paper describes the development of a new data acquisition standard for synthetic biology. This comprises the creation of a methodology that is designed to capture all the data, metadata, and protocol information associated with biopart characterization experiments. The new standard, called DICOM-SB, is based on t...

journal_title：ACS synthetic biology

authors： Sainz de Murieta I,Bultelle M,Kitney RI

更新日期：2016-08-19 00:00:00

abstract：:The extracellular expression of recombinant proteins using laboratory strains of Escherichia coli is now routinely achieved using naturally secreted substrates, such as YebF or the osmotically inducible protein Y (OsmY), as carrier molecules. However, secretion efficiency through these pathways needs to be improved fo...

journal_title：ACS synthetic biology

authors： Natarajan A,Haitjema CH,Lee R,Boock JT,DeLisa MP

更新日期：2017-05-19 00:00:00

abstract：:To facilitate the construction of cell-free genetic devices, we evaluated the ability of 17 different fluorescent proteins to give easily detectable fluorescence signals in real-time from in vitro transcription-translation reactions with a minimal system consisting of T7 RNA polymerase and E. coli translation machiner...

journal_title：ACS synthetic biology

authors： Lentini R,Forlin M,Martini L,Del Bianco C,Spencer AC,Torino D,Mansy SS

更新日期：2013-09-20 00:00:00

abstract：:While inducing agents are often used to redirect resources from growth and proliferation toward product outputs, they can be prohibitively expensive on the industrial scale. Previously, we developed an autonomously guided protein production system based on the rewiring of E. coli's native quorum sensing (QS) signal tr...

journal_title：ACS synthetic biology

authors： Zargar A,Quan DN,Bentley WE

更新日期：2016-09-16 00:00:00

abstract：:RNA parts often serve as critical components in genetic engineering. Here we report a design of translational activators which is composed of an RNA endoribonuclease (Csy4) and two exchangeable RNA modules. Csy4, a member of Cas endoribonuclease, cleaves at a specific recognition site; this cleavage releases a cis-rep...

journal_title：ACS synthetic biology

authors： Du P,Miao C,Lou Q,Wang Z,Lou C

更新日期：2016-01-15 00:00:00

abstract：:In the search for enzymatically processed RNA fragments, we found the novel three-way junction motif. The structure prediction suggested the arrangement of helices at acute angle approx. 60°. This allows the design of a trimeric RNA nanoparticle that can be functionalized with multiple regulatory fragments. Such RNA n...

journal_title：ACS synthetic biology

authors： Jedrzejczyk D,Chworos A

更新日期：2019-03-15 00:00:00

abstract：:Carotenoids, a variety of natural products, have significant pharmaceutical and commercial potential. Phytoene dehydrogenase (CrtI) is the rate-limit enzyme for carotenoid synthesis, whose catalysis specificity results in various carotenoids. However, the structural characteristics of CrtI for controlling the catalysi...

journal_title：ACS synthetic biology

authors： Liang N,Chen C,Wang Y,Ding MZ,Yao MD,Xiao WH,Yuan YJ

更新日期：2020-07-17 00:00:00