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Self-Assembling RNA Nanoparticle for Gene Expression Regulation in a Model System.

Abstract：

:In the search for enzymatically processed RNA fragments, we found the novel three-way junction motif. The structure prediction suggested the arrangement of helices at acute angle approx. 60°. This allows the design of a trimeric RNA nanoparticle that can be functionalized with multiple regulatory fragments. Such RNA nano-object of equilateral triangular shape was applied for gene expression regulation studies in two independent cellular systems. Biochemical and functional studies confirmed the predicted shape and structure of the nanoparticle. The regulatory siRNA fragments incorporated into the nanoparticle were effectively released and triggered gene silencing. The regulatory effect was prolonged when induced with structuralized RNA compared to unstructured siRNAs. In these studies, the enzymatic processing of the motif was utilized for function release from the nanoparticle, enabling simultaneous delivery of different regulatory functions. This methodology of sequence search, RNA structural prediction, and application for rational design opens a new way for creating enzymatically processed RNA nanoparticles.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Jedrzejczyk D,Chworos A

doi

10.1021/acssynbio.8b00319

subject

Has Abstract

pub_date

2019-03-15 00:00:00

pages

491-497

issue

3

issn

2161-5063

journal_volume

8

pub_type

杂志文章

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