Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function.

abstract：:Phages are biological entities found in every ecosystem. Although much has been learned about them in past decades, significant knowledge gaps remain. Manipulating virulent phage genomes is challenging. To date, no efficient gene-editing tools exist for engineering virulent lactococcal phages. Lactococcus lactis is a ...

journal_title：ACS synthetic biology

authors： Lemay ML,Tremblay DM,Moineau S

更新日期：2017-07-21 00:00:00

abstract：:Strategies to optimize a metabolic pathway often involve building a large collection of strains, each containing different versions of sequences that regulate the expression of pathway genes. Here, we develop reagents and methods to carry out this process at high efficiency in the yeast Saccharomyces cerevisiae. We id...

journal_title：ACS synthetic biology

authors： Cuperus JT,Lo RS,Shumaker L,Proctor J,Fields S

更新日期：2015-07-17 00:00:00

abstract：:The methods to execute biological experiments are evolving. Affordable fluid handling robots and on-demand biology enterprises are making automating entire experiments a reality. Automation offers the benefit of high-throughput experimentation, rapid prototyping, and improved reproducibility of results. However, learn...

journal_title：ACS synthetic biology

authors： Gupta V,Irimia J,Pau I,Rodríguez-Patón A

更新日期：2017-07-21 00:00:00

abstract：:In order to match our ability to conceive of and construct cells with enhanced function, we must concomitantly develop facile, real-time methods for elucidating performance. With these, new designs can be tested in silico and steps in construction incrementally validated. Electrochemical monitoring offers the above ad...

journal_title：ACS synthetic biology

authors： Tschirhart T,Zhou XY,Ueda H,Tsao CY,Kim E,Payne GF,Bentley WE

更新日期：2016-01-15 00:00:00

abstract：:Noncanonical amino acids form a highly diverse pool of building blocks that can render unique physicochemical properties to peptides and proteins. Here, four methionine analogues with unsaturated and varying side chain lengths were successfully incorporated at four different positions in nisin in Lactococcus lactis th...

journal_title：ACS synthetic biology

authors： Deng J,Viel JH,Chen J,Kuipers OP

更新日期：2020-09-18 00:00:00

abstract：:In vitro transcription and translation reactions have become popular for a bottom-up approach to synthetic biology. Concentrations of the mRNA intermediate are rarely determined, although knowledge of synthesis and degradation rates could facilitate rational engineering of in vitro systems. We designed binary probes t...

journal_title：ACS synthetic biology

authors： Niederholtmeyer H,Xu L,Maerkl SJ

更新日期：2013-08-16 00:00:00

abstract：:The Synthetic Biology Open Language (SBOL) is an emerging synthetic biology data exchange standard, designed primarily for unambiguous and efficient machine-to-machine communication. However, manual editing of SBOL is generally difficult for nontrivial designs. Here, we describe ShortBOL, a lightweight SBOL scripting ...

journal_title：ACS synthetic biology

authors： Crowther M,Grozinger L,Pocock M,Taylor CPD,McLaughlin JA,Mısırlı G,Bartley BA,Beal J,Goñi-Moreno A,Wipat A

更新日期：2020-04-17 00:00:00

abstract：:Optogenetic tools can provide direct and programmable control of gene expression. Light-inducible recombinases, in particular, offer a powerful method for achieving precise spatiotemporal control of DNA modification. However, to-date this technology has been largely limited to eukaryotic systems. Here, we develop opto...

journal_title：ACS synthetic biology

authors： Sheets MB,Wong WW,Dunlop MJ

更新日期：2020-02-21 00:00:00

abstract：:The availability of different host chassis will greatly expand the range of applications in synthetic biology. Members of the Acetobacteraceae family of Gram-negative bacteria form an attractive class of nonmodel microorganisms that can be exploited to produce industrial chemicals, food and beverage, and biomaterials....

journal_title：ACS synthetic biology

authors： Teh MY,Ooi KH,Danny Teo SX,Bin Mansoor ME,Shaun Lim WZ,Tan MH

更新日期：2019-04-19 00:00:00

abstract：:The versatility of Ca2+ signals allows it to regulate diverse cellular processes such as migration, apoptosis, motility and exocytosis. In some receptors (e.g., VEGFR2), Ca2+ signals are generated upon binding their ligand(s) (e.g., VEGF-A). Here, we employed a design strategy to engineer proteins that generate a Ca2+...

journal_title：ACS synthetic biology

authors： Qudrat A,Truong K

更新日期：2017-03-17 00:00:00

abstract：:Author: Recently, increasing research in siderophores has been dedicated to their possible medical applications in diagnostics and therapeutics for human pathogenic infections. Fusarinine C (FsC) is a natural hydroxamate siderophore that harbors three amino groups, which allow the easy chemical modification of FsC for...

journal_title：ACS synthetic biology

authors： Lu Y,Wang H,Wang Z,Cong Y,Zhang P,Liu G,Liu C,Chi Z,Chi Z

更新日期：2019-08-16 00:00:00

abstract：:Engineered genetic systems are commonly unstable; if propagated, they evolve to reverse or modify engineered elements because the elements impair fitness. A goal of synthetic biology is thus to anticipate and avoid detrimental engineering, but little is yet known about which types of elements cause problems in differe...

journal_title：ACS synthetic biology

authors： Springman R,Molineux IJ,Duong C,Bull RJ,Bull JJ

更新日期：2012-09-21 00:00:00

abstract：:Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different ...

journal_title：ACS synthetic biology

authors： Zhang D,Zhao Y,He Y,Wang Y,Zhao Y,Zheng Y,Wei X,Zhang L,Li Y,Jin T,Wu L,Wang H,Davison PA,Xu J,Huang WE

更新日期：2012-07-20 00:00:00

abstract：:Biological nitrogen fixation, a process unique to diazotrophic prokaryote, is catalyzed by the nitrogenase complex. There has been a long-standing interest in reconstituting a nitrogenase biosynthetic pathway in a eukaryotic host with the final aim of developing N2-fixing cereal crops. In this study, we report that a ...

journal_title：ACS synthetic biology

authors： Liu X,Wang M,Song Y,Li Y,Liu P,Shi H,Li Y,Hao T,Zhang H,Jiang W,Chen S,Li J

更新日期：2019-08-16 00:00:00

abstract：:Lactams are an important class of commodity chemicals used in the manufacture of nylons, with millions of tons produced every year. Biological production of lactams could be greatly improved by high-throughput sensors for lactam biosynthesis. To identify biosensors of lactams, we applied a chemoinformatic approach ins...

journal_title：ACS synthetic biology

authors： Zhang J,Barajas JF,Burdu M,Ruegg TL,Dias B,Keasling JD

更新日期：2017-03-17 00:00:00

abstract：:As engineering foundations such as standards and abstraction begin to mature within synthetic biology, it is vital that genetic design automation (GDA) tools be developed to enable synthetic biologists to automatically select standardized DNA components from a library to meet the behavioral specification for a genetic...

journal_title：ACS synthetic biology

authors： Roehner N,Myers CJ

更新日期：2014-08-15 00:00:00

abstract：:The measurement of gene expression using fluorescence markers has been a cornerstone of synthetic biology for the past two decades. However, the use of arbitrary units has limited the usefulness of these data for many quantitative purposes. Calibration of fluorescence measurements from flow cytometry and plate reader ...

journal_title：ACS synthetic biology

authors： Fedorec AJH,Robinson CM,Wen KY,Barnes CP

更新日期：2020-09-18 00:00:00

abstract：:The photoreceptor cryptochrome 2 (CRY2) has become a powerful optogenetic tool that allows light-inducible manipulation of various signaling pathways and cellular processes in mammalian cells with high spatiotemporal precision and ease of application. However, it has also been shown that the behavior of CRY2 under blu...

journal_title：ACS synthetic biology

authors： Che DL,Duan L,Zhang K,Cui B

更新日期：2015-10-16 00:00:00

abstract：:The engineering of bacteria to controllably deliver therapeutics is an attractive application for synthetic biology. While most synthetic gene networks have been explored within microbes, there is a need for further characterization of in vivo circuit behavior in the context of applications where the host microbes are...

journal_title：ACS synthetic biology

authors： Danino T,Lo J,Prindle A,Hasty J,Bhatia SN

更新日期：2012-10-19 00:00:00

abstract：:The microbial ability to resist stressful environmental conditions and chemical inhibitors is of great industrial and medical interest. Much of the data related to mutation-based stress resistance, however, is scattered through the academic literature, making it difficult to apply systematic analyses to this wealth of...

journal_title：ACS synthetic biology

authors： Winkler JD,Halweg-Edwards AL,Erickson KE,Choudhury A,Pines G,Gill RT

更新日期：2016-12-16 00:00:00

abstract：:A major force shaping form and patterns in biology is based in the presence of amplification mechanisms able to generate ordered, large-scale spatial structures out of local interactions and random initial conditions. Turing patterns are one of the best known candidates for such ordering dynamics, and their existence ...

journal_title：ACS synthetic biology

authors： Duran-Nebreda S,Solé RV

更新日期：2016-07-15 00:00:00

abstract：:Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors that rely on broad host range origins for bacterial replication. Here we present pLX vect...

journal_title：ACS synthetic biology

authors： Pasin F,Bedoya LC,Bernabé-Orts JM,Gallo A,Simón-Mateo C,Orzaez D,García JA

更新日期：2017-10-20 00:00:00

abstract：:Menthol isomers are high-value monoterpenoid commodity chemicals, produced naturally by mint plants, Mentha spp. Alternative clean biosynthetic routes to these compounds are commercially attractive. Optimization strategies for biocatalytic terpenoid production are mainly focused on metabolic engineering of the biosynt...

journal_title：ACS synthetic biology

authors： Toogood HS,Ní Cheallaigh A,Tait S,Mansell DJ,Jervis A,Lygidakis A,Humphreys L,Takano E,Gardiner JM,Scrutton NS

更新日期：2015-10-16 00:00:00

abstract：:Site directed RNA editing is an engineered tool for the posttranscriptional manipulation of RNA and proteins. Here, we demonstrate the inclusion of additional N- and C-terminal protein domains in an RNA editing-dependent manner to switch between protein isoforms in mammalian cell culture. By inclusion of localization ...

journal_title：ACS synthetic biology

authors： Vogel P,Hanswillemenke A,Stafforst T

更新日期：2017-09-15 00:00:00

abstract：:Eukaryotic transcriptional factors (TFs) typically recognize short genomic sequences alone or together with other proteins to modulate gene expression. Mapping of TF-DNA interactions in the genome is crucial for understanding the gene regulatory programs in cells. While chromatin immunoprecipitation followed by sequen...

journal_title：ACS synthetic biology

authors： Xiong X,Zhang Y,Yan J,Jain S,Chee S,Ren B,Zhao H

更新日期：2017-06-16 00:00:00

abstract：:Actinomycetes are important organisms for the biosynthesis of valuable natural products. However, only a limited number of well-characterized native constitutive promoters from actinomycetes are available for the construction and engineering of large biochemical pathways. Here, we report the discovery and characteriza...

journal_title：ACS synthetic biology

authors： Luo Y,Zhang L,Barton KW,Zhao H

更新日期：2015-09-18 00:00:00

abstract：:Microbial electrosynthesis is an emerging technology with the potential to simultaneously store renewably generated energy, fix carbon dioxide, and produce high-value organic compounds. However, limited understanding of the route of electrons into the cell remains an obstacle to developing a robust microbial electrosy...

journal_title：ACS synthetic biology

authors： Tefft NM,TerAvest MA

更新日期：2019-07-19 00:00:00

abstract：:A gene-directed chemical communication pathway between synthetic protocell signaling transmitters (lipid vesicles) and receivers (proteinosomes) was designed, built and tested using a bottom-up modular approach comprising small molecule transcriptional control, cell-free gene expression, porin-directed efflux, substra...

journal_title：ACS synthetic biology

authors： Tang TD,Cecchi D,Fracasso G,Accardi D,Coutable-Pennarun A,Mansy SS,Perriman AW,Anderson JLR,Mann S

更新日期：2018-02-16 00:00:00

abstract：:Microorganisms often use specific autoinducers other than common metabolites for quorum sensing (QS). Herein, we demonstrated that Escherichia coli produced sulfide (H2S, HS-, and S2-) with the concentrations proportionally correlated to its cell density. We then designed synthetic gene circuits that used H2S as an au...

journal_title：ACS synthetic biology

authors： Liu H,Fan K,Li H,Wang Q,Yang Y,Li K,Xia Y,Xun L

更新日期：2019-09-20 00:00:00

abstract：:Komagataeibacter xylinus has received increasing attention as an important microorganism for the conversion of several carbon sources to bacterial cellulose (BC). However, BC productivity has been impeded by the lack of efficient genetic engineering techniques. In this study, a lambda Red and FLP/FRT-mediated site-spe...

journal_title：ACS synthetic biology

authors： Liu LP,Yang X,Zhao XJ,Zhang KY,Li WC,Xie YY,Jia SR,Zhong C

更新日期：2020-11-20 00:00:00