Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus.

abstract：:Cell-free protein synthesis (CFPS) is a widely used research tool in systems and synthetic biology. However, if CFPS is to become a mainstream technology for applications such as point of care manufacturing, we must understand the performance limits and costs of these systems. Toward this question, we used sequence sp...

journal_title：ACS synthetic biology

authors： Vilkhovoy M,Horvath N,Shih CH,Wayman JA,Calhoun K,Swartz J,Varner JD

更新日期：2018-08-17 00:00:00

abstract：:Modern bioprocessing depends on superior cellular traits, many stemming from unknown genes and gene interactions. Tolerance to toxic chemicals is such an industrially important complex trait, which frequently limits the economic feasibility of producing commodity chemicals and biofuels. Chemical tolerance encompasses ...

journal_title：ACS synthetic biology

authors： Zingaro KA,Nicolaou SA,Yuan Y,Papoutsakis ET

更新日期：2014-07-18 00:00:00

abstract：:A major force shaping form and patterns in biology is based in the presence of amplification mechanisms able to generate ordered, large-scale spatial structures out of local interactions and random initial conditions. Turing patterns are one of the best known candidates for such ordering dynamics, and their existence ...

journal_title：ACS synthetic biology

authors： Duran-Nebreda S,Solé RV

更新日期：2016-07-15 00:00:00

abstract：:The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting step of the prototyping cycle for synthetic biology. Recently developed DNA assembly methods such as isothermal assembly and type IIS restriction enzyme systems t...

journal_title：ACS synthetic biology

authors： Halleran AD,Swaminathan A,Murray RM

更新日期：2018-05-18 00:00:00

abstract：:Bacteria of the order Actinomycetales are one of the most important sources of pharmacologically active and industrially relevant secondary metabolites. Unfortunately, many of them are still recalcitrant to genetic manipulation, which is a bottleneck for systematic metabolic engineering. To facilitate the genetic mani...

journal_title：ACS synthetic biology

authors： Tong Y,Charusanti P,Zhang L,Weber T,Lee SY

更新日期：2015-09-18 00:00:00

abstract：:For infectious diseases, rapid and accurate identification of the pathogen is critical for effective management and treatment, but diagnosis remains challenging, particularly in resource-limited areas. Methods that accurately detect pathogen nucleic acids can provide robust, accurate, rapid, and ultrasensitive technol...

journal_title：ACS synthetic biology

authors： Aman R,Mahas A,Mahfouz M

更新日期：2020-06-19 00:00:00

abstract：:Multiplex Automated Genome Engineering (MAGE) allows simultaneous mutagenesis of multiple target sites in bacterial genomes using short oligonucleotides. However, large-scale mutagenesis requires hundreds to thousands of unique oligos, which are costly to synthesize and impossible to scale-up by traditional phosphoram...

journal_title：ACS synthetic biology

authors： Bonde MT,Kosuri S,Genee HJ,Sarup-Lytzen K,Church GM,Sommer MO,Wang HH

更新日期：2015-01-16 00:00:00

abstract：:Staphylococci are prevalent skin-dwelling bacteria that are also leading causes of antibiotic-resistant infections. Viruses that infect and lyse these organisms (virulent staphylococcal phages) can be used as alternatives to conventional antibiotics and represent promising tools to eliminate or manipulate specific spe...

journal_title：ACS synthetic biology

authors： Bari SMN,Walker FC,Cater K,Aslan B,Hatoum-Aslan A

更新日期：2017-12-15 00:00:00

abstract：:Phenazine-1-carboxamide (PCN) produced by multifarious Pseudomonas strains represents a promising candidate as a new metabolite pesticide due to its broad-spectrum antifungal activity and capacity to induce systemic resistance in plants. The rice rhizosphere Pseudomonas strain PA1201 contains two reiterated gene clust...

journal_title：ACS synthetic biology

authors： Jin ZJ,Zhou L,Sun S,Cui Y,Song K,Zhang X,He YW

更新日期：2020-07-17 00:00:00

abstract：:A negating functionality is fundamental to information processing of logic circuits within cells and computers. Aiming to adapt unutilized electronic concepts to the interrogation of signaling circuits in cells, we first took a bottom-up strategy whereby we created protein-based devices that perform negating Boolean l...

journal_title：ACS synthetic biology

authors： Razavi S,Su S,Inoue T

更新日期：2014-09-19 00:00:00

abstract：:Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which ...

journal_title：ACS synthetic biology

authors： Moore R,Chandrahas A,Bleris L

更新日期：2014-10-17 00:00:00

abstract：:Synthetic biology is today harnessing the design of novel and greener biosynthesis routes for the production of added-value chemicals and natural products. The design of novel pathways often requires a detailed selection of enzyme sequences to import into the chassis at each of the reaction steps. To address such desi...

journal_title：ACS synthetic biology

authors： Mellor J,Grigoras I,Carbonell P,Faulon JL

更新日期：2016-06-17 00:00:00

abstract：:In vitro transcription and translation reactions have become popular for a bottom-up approach to synthetic biology. Concentrations of the mRNA intermediate are rarely determined, although knowledge of synthesis and degradation rates could facilitate rational engineering of in vitro systems. We designed binary probes t...

journal_title：ACS synthetic biology

authors： Niederholtmeyer H,Xu L,Maerkl SJ

更新日期：2013-08-16 00:00:00

abstract：:We study translation repression in bacteria by engineering a regulatory circuit that functions as a binding assay for RNA binding proteins (RBP) in vivo. We do so by inducing expression of a fluorescent protein-RBP chimera, together with encoding its binding site at various positions within the ribosomal initiation re...

journal_title：ACS synthetic biology

authors： Katz N,Cohen R,Solomon O,Kaufmann B,Atar O,Yakhini Z,Goldberg S,Amit R

更新日期：2018-12-21 00:00:00

abstract：:In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living ...

journal_title：ACS synthetic biology

authors： Huang Y,Xia A,Yang G,Jin F

更新日期：2018-05-18 00:00:00

abstract：:Top-down reduction of the bacterial genome to construct desired chassis cells is important for synthetic biology. However, the current progress in the field of genome reduction is greatly hindered by indispensable life-essential genes that are interspersed throughout the chromosomal loci. Here, we described a new meth...

journal_title：ACS synthetic biology

authors： Xue X,Wang T,Jiang P,Shao Y,Zhou M,Zhong L,Wu R,Zhou J,Xia H,Zhao G,Qin Z

更新日期：2015-06-19 00:00:00

abstract：:Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a practical, reproducib...

journal_title：ACS synthetic biology

authors： Didovyk A,Tonooka T,Tsimring L,Hasty J

更新日期：2017-12-15 00:00:00

abstract：:Quantification of biology's central dogma (transcription and translation) is pursued by a variety of methods. Direct, immediate, and ongoing quantification of these events is difficult to achieve. Common practice is to use fluorescent or luminescent proteins to report indirectly on prior cellular events, such as turni...

journal_title：ACS synthetic biology

authors： Wick S,Walsh DI 3rd,Bobrow J,Hamad-Schifferli K,Kong DS,Thorsen T,Mroszczyk K,Carr PA

更新日期：2019-05-17 00:00:00

abstract：:Synthetic biology holds promise to engineer systems to treat diseases. One critical, yet underexplored, facet of designing such systems is the interplay between the system and the pathogen. Understanding this interplay may be critical to increasing efficacy and overcoming resistance against the system. Using the princ...

journal_title：ACS synthetic biology

authors： Bracho OR,Manchery C,Haskell EC,Blanar CA,Smith RP

更新日期：2016-03-18 00:00:00

abstract：:Bacterial microcompartments are subcellular compartments found in many prokaryotes; they consist of a protein shell that encapsulates enzymes that perform a variety of functions. The shell protects the cell from potentially toxic intermediates and colocalizes enzymes for higher efficiency. Accordingly, it is of consid...

journal_title：ACS synthetic biology

authors： Sutter M,McGuire S,Ferlez B,Kerfeld CA

更新日期：2019-04-19 00:00:00

abstract：:Agricultural soils are often cocontaminated with multiple pesticides. Unfortunately, microorganisms isolated from natural environments do not possess the ability to simultaneously degrade different classes of pesticides. Currently, we can use the approaches of synthetic biology to create a strain endowed with various ...

journal_title：ACS synthetic biology

authors： Gong T,Liu R,Zuo Z,Che Y,Yu H,Song C,Yang C

更新日期：2016-05-20 00:00:00

abstract：:DNA circuits have been widely used to develop biological computing devices because of their high programmability and versatility. Here, we propose an architecture for the systematic construction of DNA circuits for analog computation based on DNA strand displacement. The elementary gates in our architecture include ad...

journal_title：ACS synthetic biology

authors： Song T,Garg S,Mokhtar R,Bui H,Reif J

更新日期：2016-08-19 00:00:00

abstract：:The extracellular expression of recombinant proteins using laboratory strains of Escherichia coli is now routinely achieved using naturally secreted substrates, such as YebF or the osmotically inducible protein Y (OsmY), as carrier molecules. However, secretion efficiency through these pathways needs to be improved fo...

journal_title：ACS synthetic biology

authors： Natarajan A,Haitjema CH,Lee R,Boock JT,DeLisa MP

更新日期：2017-05-19 00:00:00

abstract：:Biosensors are important components of many synthetic biology and metabolic engineering applications. Here, we report a second generation of Saccharomyces cerevisiae digoxigenin and progesterone biosensors based on destabilized dimeric ligand-binding domains that undergo ligand-induced stabilization. The biosensors, c...

journal_title：ACS synthetic biology

authors： Jester BW,Tinberg CE,Rich MS,Baker D,Fields S

更新日期：2018-10-19 00:00:00

abstract：:Robust and precise ratio control of heterogeneous phenotypes within an isogenic population is an essential task, especially in the development and differentiation of a large number of cells such as bacteria, sensory receptors, and blood cells. However, the mechanisms of such ratio control are poorly understood. Here, ...

journal_title：ACS synthetic biology

authors： Menn D,Sochor P,Goetz H,Tian XJ,Wang X

更新日期：2019-06-21 00:00:00

abstract：:While inducing agents are often used to redirect resources from growth and proliferation toward product outputs, they can be prohibitively expensive on the industrial scale. Previously, we developed an autonomously guided protein production system based on the rewiring of E. coli's native quorum sensing (QS) signal tr...

journal_title：ACS synthetic biology

authors： Zargar A,Quan DN,Bentley WE

更新日期：2016-09-16 00:00:00

abstract：:Most of the current methods for controlling the formation rate of a key protein or enzyme in cell factories rely on the manipulation of target genes within the pathway. In this article, we present a novel synthetic system for post-translational regulation of protein levels, FENIX, which provides both independent contr...

journal_title：ACS synthetic biology

authors： Durante-Rodríguez G,de Lorenzo V,Nikel PI

更新日期：2018-11-16 00:00:00

abstract：:Melatonin is a commercially attractive tryptophan-derived hormone. Here we describe a bioprocess for the production of melatonin using Escherichia coli to high titers. The first engineered strain produced 0.13 g/L of melatonin from tryptophan under fed-batch fermentation conditions. A 4-fold improvement on melatonin t...

journal_title：ACS synthetic biology

authors： Luo H,Schneider K,Christensen U,Lei Y,Herrgard M,Palsson BØ

更新日期：2020-06-19 00:00:00

abstract：:Antimycins are a family of natural products generated from a hybrid nonribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) assembly line. Although they possess an array of useful biological activities, their structural complexity makes chemical synthesis challenging, and their biosynthesis has thus far been ...

journal_title：ACS synthetic biology

authors： Liu J,Zhu X,Seipke RF,Zhang W

更新日期：2015-05-15 00:00:00

abstract：:Movement of molecules across membranes in response to a stimulus is a key component of cellular programming. Here, we characterize and manipulate the response of a protein-based piston capable of puncturing membranes in a pH-dependent manner. Our protein actuator consists of modified R bodies found in a bacterial endo...

journal_title：ACS synthetic biology

authors： Polka JK,Silver PA

更新日期：2016-04-15 00:00:00