Abstract:
:While inducing agents are often used to redirect resources from growth and proliferation toward product outputs, they can be prohibitively expensive on the industrial scale. Previously, we developed an autonomously guided protein production system based on the rewiring of E. coli's native quorum sensing (QS) signal transduction cascade. Self-secreted autoinducer, AI-2, accumulated over time and actuated recombinant gene expression-its design, co-opting the collective nature of QS-mediated behavior. We recently demonstrated that desynchronization of autoinduced intercellular feedback leads to bimodality in QS activation. In this work, we developed a new QS-enabled system with enhanced feedback to reduce cell heterogeneity. This narrows the population distribution of protein expression, leading to significant per cell and overall increases in productivity. We believe directed engineering of cell populations and/or cell consortia will offer many such opportunities in future bioprocessing applications.
journal_name
ACS Synth Bioljournal_title
ACS synthetic biologyauthors
Zargar A,Quan DN,Bentley WEdoi
10.1021/acssynbio.5b00261subject
Has Abstractpub_date
2016-09-16 00:00:00pages
923-8issue
9issn
2161-5063journal_volume
5pub_type
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