Abstract：
:Microorganisms often use specific autoinducers other than common metabolites for quorum sensing (QS). Herein, we demonstrated that Escherichia coli produced sulfide (H2S, HS-, and S2-) with the concentrations proportionally correlated to its cell density. We then designed synthetic gene circuits that used H2S as an autoinducer for quorum sensing. A sulfide/quinone oxidoreductase converted diffusible H2S to indiffusible hydrogen polysulfide (HS n H, n ≥ 2), and a gene regulator CstR sensed the latter to turn on the gene expression. We constructed three element libraries, with which 24 different circuits could be assembled for adjustable sensitivity to cell density. The H2S-mediated gene circuits endowed E. coli cells within the same batch or microcolony with highly synchronous behaviors. Using them we successfully constructed cell factories capable of an autonomous switch from growth phase to production phase. Thus, these circuits provide a new tool-kit for metabolic engineering and synthetic biology.
journal_name
ACS Synth Bioljournal_title
ACS synthetic biologyauthors
Liu H,Fan K,Li H,Wang Q,Yang Y,Li K,Xia Y,Xun Ldoi
10.1021/acssynbio.9b00210subject
Has Abstractpub_date
2019-09-20 00:00:00pages
2113-2120issue
9issn
2161-5063journal_volume
8pub_type
杂志文章abstract：:Ethylene is an industrially important compound, but more sustainable production methods are desirable. Since cellulosomes increase the ability of cellulolytic enzymes by physically linking the relevant enzymes via dockerin-cohesin interactions, in this study, we genetically engineered a chimeric cellulosome-like compl...
journal_title：ACS synthetic biology
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journal_title：ACS synthetic biology
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journal_title：ACS synthetic biology
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journal_title：ACS synthetic biology
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journal_title：ACS synthetic biology
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journal_title：ACS synthetic biology
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