Design and Characterization of an Icosahedral Protein Cage Formed by a Double-Fusion Protein Containing Three Distinct Symmetry Elements.

abstract：:While inducing agents are often used to redirect resources from growth and proliferation toward product outputs, they can be prohibitively expensive on the industrial scale. Previously, we developed an autonomously guided protein production system based on the rewiring of E. coli's native quorum sensing (QS) signal tr...

journal_title：ACS synthetic biology

authors： Zargar A,Quan DN,Bentley WE

更新日期：2016-09-16 00:00:00

abstract：:The Sc2.0 project is perhaps the largest synthetic biology project in the public domain, and ultimately aims to construct a new version of the humble brewer's yeast, Saccharomyces cerevisiae. Each year, the Sc2.0 consortium gather to discuss progress in this ambitious project and highlight new developments at the fore...

journal_title：ACS synthetic biology

authors： Walker RS,Cai Y

更新日期：2016-09-16 00:00:00

abstract：:Secondary structure formation of mRNA, caused by desynchronization of transcription and translation, is known to impact gene expression in vivo. Yet, inactivation of mRNA by secondary structures in cell-free protein expression is frequently overlooked. Transcription and translation rates are often not highly synchroni...

journal_title：ACS synthetic biology

authors： Hansen MM,Ventosa Rosquelles M,Yelleswarapu M,Maas RJ,van Vugt-Jonker AJ,Heus HA,Huck WT

更新日期：2016-12-16 00:00:00

abstract：:DNA replication is one of the central functions of the cell. The complexity of modern DNA replication systems raises a question: is it possible to achieve a simpler continuous isothermal DNA replication using fewer proteins? Here, we searched such replication using an evolutionary approach. Through a long-term serial ...

journal_title：ACS synthetic biology

authors： Okauchi H,Sakatani Y,Otsuka K,Ichihashi N

更新日期：2020-07-17 00:00:00

abstract：:Most of the current methods for controlling the formation rate of a key protein or enzyme in cell factories rely on the manipulation of target genes within the pathway. In this article, we present a novel synthetic system for post-translational regulation of protein levels, FENIX, which provides both independent contr...

journal_title：ACS synthetic biology

authors： Durante-Rodríguez G,de Lorenzo V,Nikel PI

更新日期：2018-11-16 00:00:00

abstract：:Design and implementation of synthetic biological circuits highly depends on well-characterized, robust promoters with predictable input-output responses. While great progress has been made with heterotrophic model organisms such as Escherichia coli, the available variety of tunable promoter parts for phototrophic cya...

journal_title：ACS synthetic biology

authors： Behle A,Saake P,Germann AT,Dienst D,Axmann IM

更新日期：2020-04-17 00:00:00

abstract：:We present the OpenPlant toolkit, a set of interlinked resources and techniques to develop Marchantia as testbed for bioengineering in plants. Marchantia is a liverwort, a simple plant with an open form of development that allows direct visualization of gene expression and dynamics of cellular growth in living tissues...

journal_title：ACS synthetic biology

authors： Sauret-Güeto S,Frangedakis E,Silvestri L,Rebmann M,Tomaselli M,Markel K,Delmans M,West A,Patron NJ,Haseloff J

更新日期：2020-04-17 00:00:00

abstract：:To elucidate the function of mevalonate-5-pyrophosphate decarboxylase (MVD) and farnesyl pyrophosphate synthase (FPS) in triterpene biosynthesis, the genes governing the expression of these enzymes were transformed into Panax ginseng hairy roots. All the transgenic lines showed higher expression levels of PgMVD and Pg...

journal_title：ACS synthetic biology

authors： Kim YK,Kim YB,Uddin MR,Lee S,Kim SU,Park SU

更新日期：2014-10-17 00:00:00

abstract：:DNA circuits have been widely used to develop biological computing devices because of their high programmability and versatility. Here, we propose an architecture for the systematic construction of DNA circuits for analog computation based on DNA strand displacement. The elementary gates in our architecture include ad...

journal_title：ACS synthetic biology

authors： Song T,Garg S,Mokhtar R,Bui H,Reif J

更新日期：2016-08-19 00:00:00

abstract：:One limitation of gene-directed enzyme prodrug therapy (GDEPT) is the difficulty in selectively and efficiently transducing cancer cells with the gene encoding a prodrug-converting enzyme. To circumvent this issue, we sought to move the selectivity from the gene delivery level to the protein level. We developed fusion...

journal_title：ACS synthetic biology

authors： Warren TD,Patel K,Eshleman JR,Ostermeier M

更新日期：2019-05-17 00:00:00

abstract：:Top-down reduction of the bacterial genome to construct desired chassis cells is important for synthetic biology. However, the current progress in the field of genome reduction is greatly hindered by indispensable life-essential genes that are interspersed throughout the chromosomal loci. Here, we described a new meth...

journal_title：ACS synthetic biology

authors： Xue X,Wang T,Jiang P,Shao Y,Zhou M,Zhong L,Wu R,Zhou J,Xia H,Zhao G,Qin Z

更新日期：2015-06-19 00:00:00

abstract：:In this work we report synthetic adhesins (SAs) enabling the rational design of the adhesion properties of E. coli. SAs have a modular structure comprising a stable β-domain for outer membrane anchoring and surface-exposed immunoglobulin domains with high affinity and specificity that can be selected from large repert...

journal_title：ACS synthetic biology

authors： Piñero-Lambea C,Bodelón G,Fernández-Periáñez R,Cuesta AM,Álvarez-Vallina L,Fernández LÁ

更新日期：2015-04-17 00:00:00

abstract：:The cell contents are encapsulated within a compartment, the volume of which is a fundamental physical parameter that may affect intracompartmental reactions. However, there have been few studies to elucidate whether and how volume changes alone can affect the reaction kinetics. It is difficult to address these questi...

journal_title：ACS synthetic biology

authors： Matsuura T,Hosoda K,Kazuta Y,Ichihashi N,Suzuki H,Yomo T

更新日期：2012-09-21 00:00:00

abstract：:Multiplex Automated Genome Engineering (MAGE) allows simultaneous mutagenesis of multiple target sites in bacterial genomes using short oligonucleotides. However, large-scale mutagenesis requires hundreds to thousands of unique oligos, which are costly to synthesize and impossible to scale-up by traditional phosphoram...

journal_title：ACS synthetic biology

authors： Bonde MT,Kosuri S,Genee HJ,Sarup-Lytzen K,Church GM,Sommer MO,Wang HH

更新日期：2015-01-16 00:00:00

abstract：:Although recent advances in synthetic biology allow us to produce biological designs more efficiently than ever, our ability to predict the end result of these designs is still nascent. Predictive models require large amounts of high-quality data to be parametrized and tested, which are not generally available. Here, ...

journal_title：ACS synthetic biology

authors： Morrell WC,Birkel GW,Forrer M,Lopez T,Backman TWH,Dussault M,Petzold CJ,Baidoo EEK,Costello Z,Ando D,Alonso-Gutierrez J,George KW,Mukhopadhyay A,Vaino I,Keasling JD,Adams PD,Hillson NJ,Garcia Martin H

更新日期：2017-12-15 00:00:00

abstract：:Although Escherichia coli has been a popular tool for plasmid construction, this bacterium was believed to be "unsuitable" for constructing a large plasmid whose size exceeds 500 kilobases. We assumed that traditional plasmid vectors may lack some regulatory DNA elements required for the stable replication and segrega...

journal_title：ACS synthetic biology

authors： Mukai T,Yoneji T,Yamada K,Fujita H,Nara S,Su'etsugu M

更新日期：2020-06-19 00:00:00

abstract：:Robust and precise ratio control of heterogeneous phenotypes within an isogenic population is an essential task, especially in the development and differentiation of a large number of cells such as bacteria, sensory receptors, and blood cells. However, the mechanisms of such ratio control are poorly understood. Here, ...

journal_title：ACS synthetic biology

authors： Menn D,Sochor P,Goetz H,Tian XJ,Wang X

更新日期：2019-06-21 00:00:00

abstract：:The ATLAS of Biochemistry is a repository of both known and novel predicted biochemical reactions between biological compounds listed in the Kyoto Encyclopedia of Genes and Genomes (KEGG). ATLAS was originally compiled based on KEGG 2015, though the number of KEGG reactions has increased by almost 20 percent since the...

journal_title：ACS synthetic biology

authors： Hafner J,MohammadiPeyhani H,Sveshnikova A,Scheidegger A,Hatzimanikatis V

更新日期：2020-06-19 00:00:00

abstract：:Antimycins are a family of natural products generated from a hybrid nonribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) assembly line. Although they possess an array of useful biological activities, their structural complexity makes chemical synthesis challenging, and their biosynthesis has thus far been ...

journal_title：ACS synthetic biology

authors： Liu J,Zhu X,Seipke RF,Zhang W

更新日期：2015-05-15 00:00:00

abstract：:We have previously engineered green/red and red/far red photoreversible E. coli phytochrome and cyanobacteriochrome (CBCR) two-component systems (TCSs) and utilized them to program tailor-made gene expression signals for gene circuit characterization. Here, we transport the UV-violet/green photoreversible CBCR TCS Uir...

journal_title：ACS synthetic biology

authors： Ramakrishnan P,Tabor JJ

更新日期：2016-07-15 00:00:00

abstract：:Bacterial microcompartments are subcellular compartments found in many prokaryotes; they consist of a protein shell that encapsulates enzymes that perform a variety of functions. The shell protects the cell from potentially toxic intermediates and colocalizes enzymes for higher efficiency. Accordingly, it is of consid...

journal_title：ACS synthetic biology

authors： Sutter M,McGuire S,Ferlez B,Kerfeld CA

更新日期：2019-04-19 00:00:00

abstract：:Novel approaches are needed to combat antibiotic resistance. Here, we describe a computational-experimental framework for the discovery of novel cryptic antimicrobial peptides (AMPs). The computational platform, based on previously validated antimicrobial scoring functions, indicated the activation peptide of pepsin A...

journal_title：ACS synthetic biology

authors： Pane K,Cafaro V,Avitabile A,Torres MT,Vollaro A,De Gregorio E,Catania MR,Di Maro A,Bosso A,Gallo G,Zanfardino A,Varcamonti M,Pizzo E,Di Donato A,Lu TK,de la Fuente-Nunez C,Notomista E

更新日期：2018-09-21 00:00:00

abstract：:Scarless genetic manipulation of genomes is an essential tool for biological research. The restriction-modification (R-M) system is a defense system in bacteria that protects against invading genomes on the basis of its ability to distinguish foreign DNA from self DNA. Here, we designed an R-M system-mediated genome e...

journal_title：ACS synthetic biology

authors： Bai H,Deng A,Liu S,Cui D,Qiu Q,Wang L,Yang Z,Wu J,Shang X,Zhang Y,Wen T

更新日期：2018-01-19 00:00:00

abstract：:In the search for enzymatically processed RNA fragments, we found the novel three-way junction motif. The structure prediction suggested the arrangement of helices at acute angle approx. 60°. This allows the design of a trimeric RNA nanoparticle that can be functionalized with multiple regulatory fragments. Such RNA n...

journal_title：ACS synthetic biology

authors： Jedrzejczyk D,Chworos A

更新日期：2019-03-15 00:00:00

abstract：:In vitro transcription and translation reactions have become popular for a bottom-up approach to synthetic biology. Concentrations of the mRNA intermediate are rarely determined, although knowledge of synthesis and degradation rates could facilitate rational engineering of in vitro systems. We designed binary probes t...

journal_title：ACS synthetic biology

authors： Niederholtmeyer H,Xu L,Maerkl SJ

更新日期：2013-08-16 00:00:00

abstract：:RNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamer-fluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA),...

journal_title：ACS synthetic biology

authors： Yerramilli VS,Kim KH

更新日期：2018-03-16 00:00:00

abstract：:Dramatic improvements to computational, robotic, and biological tools have enabled genetic engineers to conduct increasingly sophisticated experiments. Further development of biological tools offers a route to bypass complex or expensive mechanical operations, thereby reducing the time and cost of highly parallelized ...

journal_title：ACS synthetic biology

authors： Kittleson JT,DeLoache W,Cheng HY,Anderson JC

更新日期：2012-12-21 00:00:00

abstract：:Allosteric proteins, which can sense different signals, are interesting biological parts for synthetic biology. In particular, the design of an artificial allosteric enzyme to sense an unnatural signal is both challenging and highly desired, for example, for a precise and dynamical control of fluxes of growth-essentia...

journal_title：ACS synthetic biology

authors： Chen Z,Rappert S,Zeng AP

更新日期：2015-02-20 00:00:00

abstract：:As light-regulated actuators, sensory photoreceptors underpin optogenetics and numerous applications in synthetic biology. Protein engineering has been applied to fine-tune the properties of photoreceptors and to generate novel actuators. For the blue-light-sensitive light-oxygen-voltage (LOV) photoreceptors, mutation...

journal_title：ACS synthetic biology

authors： Diensthuber RP,Engelhard C,Lemke N,Gleichmann T,Ohlendorf R,Bittl R,Möglich A

更新日期：2014-11-21 00:00:00

abstract：:Melatonin is a commercially attractive tryptophan-derived hormone. Here we describe a bioprocess for the production of melatonin using Escherichia coli to high titers. The first engineered strain produced 0.13 g/L of melatonin from tryptophan under fed-batch fermentation conditions. A 4-fold improvement on melatonin t...

journal_title：ACS synthetic biology

authors： Luo H,Schneider K,Christensen U,Lei Y,Herrgard M,Palsson BØ

更新日期：2020-06-19 00:00:00