当前位置： SCI文献检索 > ACS Synthetic Biology期刊下所有文献 > Engineered Biosensors from Dimeric Ligand-Binding Domains.

Engineered Biosensors from Dimeric Ligand-Binding Domains.

Abstract：

:Biosensors are important components of many synthetic biology and metabolic engineering applications. Here, we report a second generation of Saccharomyces cerevisiae digoxigenin and progesterone biosensors based on destabilized dimeric ligand-binding domains that undergo ligand-induced stabilization. The biosensors, comprising one ligand-binding domain monomer fused to a DNA-binding domain and another fused to a transcriptional activation domain, activate reporter gene expression in response to steroid binding and receptor dimerization. The introduction of a destabilizing mutation to the dimer interface increased biosensor dynamic range by an order of magnitude. Computational redesign of the dimer interface and functional selections were used to create heterodimeric pairs with further improved dynamic range. A heterodimeric biosensor built from the digoxigenin and progesterone ligand-binding domains functioned as a synthetic "AND"-gate, with 20-fold stronger response to the two ligands in combination than to either one alone. We also identified mutations that increase the sensitivity or selectivity of the biosensors to chemically similar ligands. These dimerizing biosensors provide additional flexibility for the construction of logic gates and other applications.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Jester BW,Tinberg CE,Rich MS,Baker D,Fields S

doi

10.1021/acssynbio.8b00242

subject

Has Abstract

pub_date

2018-10-19 00:00:00

pages

2457-2467

issue

10

issn

2161-5063

journal_volume

7

pub_type

杂志文章

相关文献

ACS Synthetic Biology文献大全
  • Circumvention of Learning Increases Intoxication Efficacy of Nematicidal Engineered Bacteria.

    abstract：:Synthetic biology holds promise to engineer systems to treat diseases. One critical, yet underexplored, facet of designing such systems is the interplay between the system and the pathogen. Understanding this interplay may be critical to increasing efficacy and overcoming resistance against the system. Using the princ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.5b00192

    authors： Bracho OR,Manchery C,Haskell EC,Blanar CA,Smith RP

    更新日期：2016-03-18 00:00:00

  • An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae.

    abstract：:The availability of different host chassis will greatly expand the range of applications in synthetic biology. Members of the Acetobacteraceae family of Gram-negative bacteria form an attractive class of nonmodel microorganisms that can be exploited to produce industrial chemicals, food and beverage, and biomaterials....

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00168

    authors： Teh MY,Ooi KH,Danny Teo SX,Bin Mansoor ME,Shaun Lim WZ,Tan MH

    更新日期：2019-04-19 00:00:00

  • A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis.

    abstract：:Eukaryotic transcriptional factors (TFs) typically recognize short genomic sequences alone or together with other proteins to modulate gene expression. Mapping of TF-DNA interactions in the genome is crucial for understanding the gene regulatory programs in cells. While chromatin immunoprecipitation followed by sequen...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.6b00358

    authors： Xiong X,Zhang Y,Yan J,Jain S,Chee S,Ren B,Zhao H

    更新日期：2017-06-16 00:00:00

  • Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production.

    abstract：:Type I modular polyketide synthases (PKSs) are polymerases that utilize acyl-CoAs as substrates. Each polyketide elongation reaction is catalyzed by a set of protein domains called a module. Each module usually contains an acyltransferase (AT) domain, which determines the specific acyl-CoA incorporated into each conde...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.6b00176

    authors： Yuzawa S,Deng K,Wang G,Baidoo EE,Northen TR,Adams PD,Katz L,Keasling JD

    更新日期：2017-01-20 00:00:00

  • Identification of Novel Cryptic Multifunctional Antimicrobial Peptides from the Human Stomach Enabled by a Computational-Experimental Platform.

    abstract：:Novel approaches are needed to combat antibiotic resistance. Here, we describe a computational-experimental framework for the discovery of novel cryptic antimicrobial peptides (AMPs). The computational platform, based on previously validated antimicrobial scoring functions, indicated the activation peptide of pepsin A...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00084

    authors： Pane K,Cafaro V,Avitabile A,Torres MT,Vollaro A,De Gregorio E,Catania MR,Di Maro A,Bosso A,Gallo G,Zanfardino A,Varcamonti M,Pizzo E,Di Donato A,Lu TK,de la Fuente-Nunez C,Notomista E

    更新日期：2018-09-21 00:00:00

  • Bioprinting Living Biofilms through Optogenetic Manipulation.

    abstract：:In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00003

    authors： Huang Y,Xia A,Yang G,Jin F

    更新日期：2018-05-18 00:00:00

  • Controlling Heterogeneity and Increasing Titer from Riboswitch-Regulated Bacillus subtilis Spores for Time-Delayed Protein Expression Applications.

    abstract：:Sporulated cells have potential as time-delayed expression chassis of proteins for applications such as "on-demand" biologics production, whole cell biosensors, or oral vaccines. However, the desired attributes of high expression rates and low product variances are difficult to maintain from germinated spores. In this...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00163

    authors： Tamiev D,Lantz A,Vezeau G,Salis H,Reuel NF

    更新日期：2019-10-18 00:00:00

  • Direct mutagenesis of thousands of genomic targets using microarray-derived oligonucleotides.

    abstract：:Multiplex Automated Genome Engineering (MAGE) allows simultaneous mutagenesis of multiple target sites in bacterial genomes using short oligonucleotides. However, large-scale mutagenesis requires hundreds to thousands of unique oligos, which are costly to synthesize and impossible to scale-up by traditional phosphoram...

    journal_title：ACS synthetic biology

    pub_type： 信件

    doi：10.1021/sb5001565

    authors： Bonde MT,Kosuri S,Genee HJ,Sarup-Lytzen K,Church GM,Sommer MO,Wang HH

    更新日期：2015-01-16 00:00:00

  • 3D Printing for the Fabrication of Biofilm-Based Functional Living Materials.

    abstract：:Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00192

    authors： Balasubramanian S,Aubin-Tam ME,Meyer AS

    更新日期：2019-07-19 00:00:00

  • GeneORator: An Effective Strategy for Navigating Protein Sequence Space More Efficiently through Boolean OR-Type DNA Libraries.

    abstract：:Directed evolution requires the creation of genetic diversity and subsequent screening or selection for improved variants. For DNA mutagenesis, conventional site-directed methods implicitly utilize the Boolean AND operator (creating all mutations simultaneously), producing a combinatorial explosion in the number of ge...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00063

    authors： Currin A,Kwok J,Sadler JC,Bell EL,Swainston N,Ababi M,Day P,Turner NJ,Kell DB

    更新日期：2019-06-21 00:00:00

  • Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli.

    abstract：:Although Escherichia coli has been a popular tool for plasmid construction, this bacterium was believed to be "unsuitable" for constructing a large plasmid whose size exceeds 500 kilobases. We assumed that traditional plasmid vectors may lack some regulatory DNA elements required for the stable replication and segrega...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00008

    authors： Mukai T,Yoneji T,Yamada K,Fujita H,Nara S,Su'etsugu M

    更新日期：2020-06-19 00:00:00

  • Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies.

    abstract：:Bacterial microcompartments are subcellular compartments found in many prokaryotes; they consist of a protein shell that encapsulates enzymes that perform a variety of functions. The shell protects the cell from potentially toxic intermediates and colocalizes enzymes for higher efficiency. Accordingly, it is of consid...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00011

    authors： Sutter M,McGuire S,Ferlez B,Kerfeld CA

    更新日期：2019-04-19 00:00:00

  • Engineered Bacterial Production of Volatile Methyl Salicylate.

    abstract：:The engineering of microbial metabolic pathways over the last two decades has led to numerous examples of cell factories used for the production of small molecules. These molecules have an array of utility in commercial industries and as in situ expressed biomarkers or therapeutics in microbial applications. While mos...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00497

    authors： Chien T,Jones DR,Danino T

    更新日期：2021-01-15 00:00:00

  • Curli-Mediated Self-Assembly of a Fibrous Protein Scaffold for Hydroxyapatite Mineralization.

    abstract：:Nanostructures formed by self-assembled peptides have been increasingly exploited as functional materials for a wide variety of applications, from biotechnology to energy. However, it is sometimes challenging to assemble free short peptides into functional supramolecular structures, since not all peptides have the abi...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00415

    authors： Abdali Z,Aminzare M,Zhu X,DeBenedictis E,Xie O,Keten S,Dorval Courchesne NM

    更新日期：2020-12-18 00:00:00

  • Self-Assembling RNA Nanoparticle for Gene Expression Regulation in a Model System.

    abstract：:In the search for enzymatically processed RNA fragments, we found the novel three-way junction motif. The structure prediction suggested the arrangement of helices at acute angle approx. 60°. This allows the design of a trimeric RNA nanoparticle that can be functionalized with multiple regulatory fragments. Such RNA n...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00319

    authors： Jedrzejczyk D,Chworos A

    更新日期：2019-03-15 00:00:00

  • An Orthogonal Multi-input Integration System to Control Gene Expression in Escherichia coli.

    abstract：:In many biotechnological applications, it is useful for gene expression to be regulated by multiple signals, as this allows the programming of complex behavior. Here we implement, in Escherichia coli, a system that compares the concentration of two signal molecules, and tunes GFP expression proportionally to their rel...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.7b00109

    authors： Annunziata F,Matyjaszkiewicz A,Fiore G,Grierson CS,Marucci L,di Bernardo M,Savery NJ

    更新日期：2017-10-20 00:00:00

  • Exploiting Spermidine N-Hydroxycinnamoyltransferase Diversity and Substrate Promiscuity to Produce Various Trihydroxycinnamoyl Spermidines and Analogues in Engineered Yeast.

    abstract：:Trihydroxycinnamoyl spermidines (THCSpd) are plant specialized metabolites with promising pharmacological activities as antifungals, antibacterial, antiviral, and antidepressant drugs. However, their characterization and potential pharmaceutical exploitation are greatly impaired by the sourcing of these compounds, res...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.0c00391

    authors： Perrin J,Kulagina N,Unlubayir M,Munsch T,Carqueijeiro I,Dugé de Bernonville T,De Craene JO,Clastre M,St-Pierre B,Giglioli-Guivarc'h N,Gagneul D,Lanoue A,Courdavault V,Besseau S

    更新日期：2021-01-15 00:00:00

  • Synthetic tunable amplifying buffer circuit in E. coli.

    abstract：:While predictable design of a genetic circuit's output is a major goal of synthetic biology, it remains a significant challenge because DNA binding sites in the cell affect the concentration of available transcription factors (TF). To mitigate this problem, we propose to use a TF that results from the (reversible) pho...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb5002533

    authors： Nilgiriwala KS,Jiménez J,Rivera PM,Del Vecchio D

    更新日期：2015-05-15 00:00:00

  • An in Vivo Binding Assay for RNA-Binding Proteins Based on Repression of a Reporter Gene.

    abstract：:We study translation repression in bacteria by engineering a regulatory circuit that functions as a binding assay for RNA binding proteins (RBP) in vivo. We do so by inducing expression of a fluorescent protein-RBP chimera, together with encoding its binding site at various positions within the ribosomal initiation re...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00378

    authors： Katz N,Cohen R,Solomon O,Kaufmann B,Atar O,Yakhini Z,Goldberg S,Amit R

    更新日期：2018-12-21 00:00:00

  • Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1.

    abstract：:Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb3000244

    authors： Zhang D,Zhao Y,He Y,Wang Y,Zhao Y,Zheng Y,Wei X,Zhang L,Li Y,Jin T,Wu L,Wang H,Davison PA,Xu J,Huang WE

    更新日期：2012-07-20 00:00:00

  • Design and Characterization of an Icosahedral Protein Cage Formed by a Double-Fusion Protein Containing Three Distinct Symmetry Elements.

    abstract：:Exploiting simple types of symmetry common to many natural protein oligomers as a starting point, several recent studies have succeeded in engineering complex self-assembling protein architectures reminiscent but distinct from those evolved in the natural world. Designing symmetric protein cages with a wide range of p...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00392

    authors： Cannon KA,Nguyen VN,Morgan C,Yeates TO

    更新日期：2020-03-20 00:00:00

  • Transcription activator-like effectors: a toolkit for synthetic biology.

    abstract：:Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which ...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章,评审

    doi：10.1021/sb400137b

    authors： Moore R,Chandrahas A,Bleris L

    更新日期：2014-10-17 00:00:00

  • Labeling RNAs in Live Cells Using Malachite Green Aptamer Scaffolds as Fluorescent Probes.

    abstract：:RNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamer-fluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA),...

    journal_title：ACS synthetic biology

    pub_type： 信件

    doi：10.1021/acssynbio.7b00237

    authors： Yerramilli VS,Kim KH

    更新日期：2018-03-16 00:00:00

  • Enzymatic Menthol Production: One-Pot Approach Using Engineered Escherichia coli.

    abstract：:Menthol isomers are high-value monoterpenoid commodity chemicals, produced naturally by mint plants, Mentha spp. Alternative clean biosynthetic routes to these compounds are commercially attractive. Optimization strategies for biocatalytic terpenoid production are mainly focused on metabolic engineering of the biosynt...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.5b00092

    authors： Toogood HS,Ní Cheallaigh A,Tait S,Mansell DJ,Jervis A,Lygidakis A,Humphreys L,Takano E,Gardiner JM,Scrutton NS

    更新日期：2015-10-16 00:00:00

  • Refactoring and optimization of light-switchable Escherichia coli two-component systems.

    abstract：:Light-switchable proteins enable unparalleled control of molecular biological processes in live organisms. Previously, we have engineered red/far-red and green/red photoreversible two-component signal transduction systems (TCSs) with transcriptional outputs in E. coli and used them to characterize and control syntheti...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb500273n

    authors： Schmidl SR,Sheth RU,Wu A,Tabor JJ

    更新日期：2014-11-21 00:00:00

  • Cloning, Stability, and Modification of Mycoplasma hominis Genome in Yeast.

    abstract：:Mycoplasma hominis is a minimal human pathogen that is responsible for genital and neonatal infections. Despite many attempts, there is no efficient genetic tool to manipulate this bacterium, limiting most investigations of its pathogenicity and its uncommon energy metabolism that relies on arginine. The recent clonin...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.6b00379

    authors： Rideau F,Le Roy C,Descamps ECT,Renaudin H,Lartigue C,Bébéar C

    更新日期：2017-05-19 00:00:00

  • Directed acyclic graph-based technology mapping of genetic circuit models.

    abstract：:As engineering foundations such as standards and abstraction begin to mature within synthetic biology, it is vital that genetic design automation (GDA) tools be developed to enable synthetic biologists to automatically select standardized DNA components from a library to meet the behavioral specification for a genetic...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb400135t

    authors： Roehner N,Myers CJ

    更新日期：2014-08-15 00:00:00

  • Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly.

    abstract：:Synthetic biology relies on the manufacture of large and complex DNA constructs from libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent DNA assembly methods enable rapid construction of genes and operons through one-pot, multifragment assembly, with the ordering of parts determined...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.8b00333

    authors： Potapov V,Ong JL,Kucera RB,Langhorst BW,Bilotti K,Pryor JM,Cantor EJ,Canton B,Knight TF,Evans TC Jr,Lohman GJS

    更新日期：2018-11-16 00:00:00

  • Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B12 Biosensor.

    abstract：:Cell-free systems provide a versatile platform for the development of low-cost, easy-to-use sensors for diverse analytes. However, sensor affinity dictates response sensitivity, and improving binding affinity can be challenging. Here, we describe efforts to address this problem while developing a biosensor for vitamin...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/acssynbio.9b00429

    authors： McNerney MP,Piorino F,Michel CL,Styczynski MP

    更新日期：2020-02-21 00:00:00

  • Enhanced triterpene accumulation in Panax ginseng hairy roots overexpressing mevalonate-5-pyrophosphate decarboxylase and farnesyl pyrophosphate synthase.

    abstract：:To elucidate the function of mevalonate-5-pyrophosphate decarboxylase (MVD) and farnesyl pyrophosphate synthase (FPS) in triterpene biosynthesis, the genes governing the expression of these enzymes were transformed into Panax ginseng hairy roots. All the transgenic lines showed higher expression levels of PgMVD and Pg...

    journal_title：ACS synthetic biology

    pub_type： 杂志文章

    doi：10.1021/sb400194g

    authors： Kim YK,Kim YB,Uddin MR,Lee S,Kim SU,Park SU

    更新日期：2014-10-17 00:00:00