Transcription activator-like effectors: a toolkit for synthetic biology.

abstract：:Strain engineering for synthetic biology and metabolic engineering applications often requires the expression of foreign proteins that can reduce cellular fitness. In order to quantify and visualize the evolutionary stability dynamics in engineered populations of Escherichia coli , we constructed randomized CMY (cyan-...

journal_title：ACS synthetic biology

authors： Sleight SC,Sauro HM

更新日期：2013-09-20 00:00:00

abstract：:Crossbreeding is a classical yeast hybridization procedure, where the mating of haploid cells of opposite mating-type, MATa and MATα cells, produces a new heterozygous diploid. Here, we describe a method to generate haploid MATa and MATα cells using mating-type conversion caused by expression of the HO gene, which enc...

journal_title：ACS synthetic biology

authors： Fukuda N,Matsukura S,Honda S

更新日期：2013-12-20 00:00:00

abstract：:Allosteric proteins, which can sense different signals, are interesting biological parts for synthetic biology. In particular, the design of an artificial allosteric enzyme to sense an unnatural signal is both challenging and highly desired, for example, for a precise and dynamical control of fluxes of growth-essentia...

journal_title：ACS synthetic biology

authors： Chen Z,Rappert S,Zeng AP

更新日期：2015-02-20 00:00:00

abstract：:Design and implementation of synthetic biological circuits highly depends on well-characterized, robust promoters with predictable input-output responses. While great progress has been made with heterotrophic model organisms such as Escherichia coli, the available variety of tunable promoter parts for phototrophic cya...

journal_title：ACS synthetic biology

authors： Behle A,Saake P,Germann AT,Dienst D,Axmann IM

更新日期：2020-04-17 00:00:00

abstract：:The engineering of bacteria to controllably deliver therapeutics is an attractive application for synthetic biology. While most synthetic gene networks have been explored within microbes, there is a need for further characterization of in vivo circuit behavior in the context of applications where the host microbes are...

journal_title：ACS synthetic biology

authors： Danino T,Lo J,Prindle A,Hasty J,Bhatia SN

更新日期：2012-10-19 00:00:00

abstract：:Type I modular polyketide synthases (PKSs) are polymerases that utilize acyl-CoAs as substrates. Each polyketide elongation reaction is catalyzed by a set of protein domains called a module. Each module usually contains an acyltransferase (AT) domain, which determines the specific acyl-CoA incorporated into each conde...

journal_title：ACS synthetic biology

authors： Yuzawa S,Deng K,Wang G,Baidoo EE,Northen TR,Adams PD,Katz L,Keasling JD

更新日期：2017-01-20 00:00:00

abstract：:Scarless genetic manipulation of genomes is an essential tool for biological research. The restriction-modification (R-M) system is a defense system in bacteria that protects against invading genomes on the basis of its ability to distinguish foreign DNA from self DNA. Here, we designed an R-M system-mediated genome e...

journal_title：ACS synthetic biology

authors： Bai H,Deng A,Liu S,Cui D,Qiu Q,Wang L,Yang Z,Wu J,Shang X,Zhang Y,Wen T

更新日期：2018-01-19 00:00:00

abstract：:To elucidate the function of mevalonate-5-pyrophosphate decarboxylase (MVD) and farnesyl pyrophosphate synthase (FPS) in triterpene biosynthesis, the genes governing the expression of these enzymes were transformed into Panax ginseng hairy roots. All the transgenic lines showed higher expression levels of PgMVD and Pg...

journal_title：ACS synthetic biology

authors： Kim YK,Kim YB,Uddin MR,Lee S,Kim SU,Park SU

更新日期：2014-10-17 00:00:00

abstract：:Engineered genetic systems are commonly unstable; if propagated, they evolve to reverse or modify engineered elements because the elements impair fitness. A goal of synthetic biology is thus to anticipate and avoid detrimental engineering, but little is yet known about which types of elements cause problems in differe...

journal_title：ACS synthetic biology

authors： Springman R,Molineux IJ,Duong C,Bull RJ,Bull JJ

更新日期：2012-09-21 00:00:00

abstract：:In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living ...

journal_title：ACS synthetic biology

authors： Huang Y,Xia A,Yang G,Jin F

更新日期：2018-05-18 00:00:00

abstract：:Trihydroxycinnamoyl spermidines (THCSpd) are plant specialized metabolites with promising pharmacological activities as antifungals, antibacterial, antiviral, and antidepressant drugs. However, their characterization and potential pharmaceutical exploitation are greatly impaired by the sourcing of these compounds, res...

journal_title：ACS synthetic biology

authors： Perrin J,Kulagina N,Unlubayir M,Munsch T,Carqueijeiro I,Dugé de Bernonville T,De Craene JO,Clastre M,St-Pierre B,Giglioli-Guivarc'h N,Gagneul D,Lanoue A,Courdavault V,Besseau S

更新日期：2021-01-15 00:00:00

abstract：:Understanding the molecular mechanisms underlying antibiotic resistance requires concerted efforts in enzymology and medicinal chemistry. Here we describe a new synthetic biology approach to antibiotic development, where the presence of tetracycline antibiotics is linked to a life-death selection in Saccharomyces cere...

journal_title：ACS synthetic biology

authors： Scott LH,Mathews JC,Flematti GR,Filipovska A,Rackham O

更新日期：2018-08-17 00:00:00

abstract：:Erythromycin is necessary in medical treatment and known to be biosynthesized with propionyl-CoA as direct precursor. Oversupply of propionyl-CoA induced hyperpropionylation, which was demonstrated as harmful for erythromycin synthesis in Saccharopolyspora erythraea. Herein, we identified three propionyl-CoA synthetas...

journal_title：ACS synthetic biology

authors： You D,Wang MM,Yin BC,Ye BC

更新日期：2019-02-15 00:00:00

abstract：:As a product of a multistep enzymatic reaction, accumulation of poly(3-hydroxybutyrate) (PHB) in Escherichia coli (E. coli) can be achieved by overexpression of the PHB synthesis pathway from a native producer involving three genes phbC, phbA, and phbB. Pathway optimization by adjusting expression levels of the three ...

journal_title：ACS synthetic biology

authors： Li T,Ye J,Shen R,Zong Y,Zhao X,Lou C,Chen GQ

更新日期：2016-11-18 00:00:00

abstract：:We present the OpenPlant toolkit, a set of interlinked resources and techniques to develop Marchantia as testbed for bioengineering in plants. Marchantia is a liverwort, a simple plant with an open form of development that allows direct visualization of gene expression and dynamics of cellular growth in living tissues...

journal_title：ACS synthetic biology

authors： Sauret-Güeto S,Frangedakis E,Silvestri L,Rebmann M,Tomaselli M,Markel K,Delmans M,West A,Patron NJ,Haseloff J

更新日期：2020-04-17 00:00:00

abstract：:Cell-free systems, as part of the synthetic biology field, have become a critical platform in biological studies. However, there is a lack of research into developing a switch for a dynamical control of the transcriptional and translational process. The optogenetic tool has been widely proven as an ideal control switc...

journal_title：ACS synthetic biology

authors： Zhang P,Yang J,Cho E,Lu Y

更新日期：2020-08-21 00:00:00

abstract：:Site directed RNA editing is an engineered tool for the posttranscriptional manipulation of RNA and proteins. Here, we demonstrate the inclusion of additional N- and C-terminal protein domains in an RNA editing-dependent manner to switch between protein isoforms in mammalian cell culture. By inclusion of localization ...

journal_title：ACS synthetic biology

authors： Vogel P,Hanswillemenke A,Stafforst T

更新日期：2017-09-15 00:00:00

abstract：:Although recent advances in synthetic biology allow us to produce biological designs more efficiently than ever, our ability to predict the end result of these designs is still nascent. Predictive models require large amounts of high-quality data to be parametrized and tested, which are not generally available. Here, ...

journal_title：ACS synthetic biology

authors： Morrell WC,Birkel GW,Forrer M,Lopez T,Backman TWH,Dussault M,Petzold CJ,Baidoo EEK,Costello Z,Ando D,Alonso-Gutierrez J,George KW,Mukhopadhyay A,Vaino I,Keasling JD,Adams PD,Hillson NJ,Garcia Martin H

更新日期：2017-12-15 00:00:00

abstract：:Actinomycetes are important organisms for the biosynthesis of valuable natural products. However, only a limited number of well-characterized native constitutive promoters from actinomycetes are available for the construction and engineering of large biochemical pathways. Here, we report the discovery and characteriza...

journal_title：ACS synthetic biology

authors： Luo Y,Zhang L,Barton KW,Zhao H

更新日期：2015-09-18 00:00:00

abstract：:The measurement of gene expression using fluorescence markers has been a cornerstone of synthetic biology for the past two decades. However, the use of arbitrary units has limited the usefulness of these data for many quantitative purposes. Calibration of fluorescence measurements from flow cytometry and plate reader ...

journal_title：ACS synthetic biology

authors： Fedorec AJH,Robinson CM,Wen KY,Barnes CP

更新日期：2020-09-18 00:00:00

abstract：:Materials synthesized by organisms, such as bones and wood, combine the ability to self-repair with remarkable mechanical properties. This multifunctionality arises from the presence of living cells within the material and hierarchical assembly of different components across nanometer to micron scales. While creating ...

journal_title：ACS synthetic biology

authors： Charrier M,Li D,Mann VR,Yun L,Jani S,Rad B,Cohen BE,Ashby PD,Ryan KR,Ajo-Franklin CM

更新日期：2019-01-18 00:00:00

abstract：:As engineering foundations such as standards and abstraction begin to mature within synthetic biology, it is vital that genetic design automation (GDA) tools be developed to enable synthetic biologists to automatically select standardized DNA components from a library to meet the behavioral specification for a genetic...

journal_title：ACS synthetic biology

authors： Roehner N,Myers CJ

更新日期：2014-08-15 00:00:00

abstract：:Episodic gene expression, with periods of high expression separated by periods of no expression, is a pervasive biological phenomenon. This bursty pattern of expression draws from a finite reservoir of expression machinery in a highly time variant way, i.e., requiring no resources most of the time but drawing heavily ...

journal_title：ACS synthetic biology

authors： Caveney PM,Norred SE,Chin CW,Boreyko JB,Razooky BS,Retterer ST,Collier CP,Simpson ML

更新日期：2017-02-17 00:00:00

abstract：:One limitation of gene-directed enzyme prodrug therapy (GDEPT) is the difficulty in selectively and efficiently transducing cancer cells with the gene encoding a prodrug-converting enzyme. To circumvent this issue, we sought to move the selectivity from the gene delivery level to the protein level. We developed fusion...

journal_title：ACS synthetic biology

authors： Warren TD,Patel K,Eshleman JR,Ostermeier M

更新日期：2019-05-17 00:00:00

abstract：:Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different ...

journal_title：ACS synthetic biology

authors： Zhang D,Zhao Y,He Y,Wang Y,Zhao Y,Zheng Y,Wei X,Zhang L,Li Y,Jin T,Wu L,Wang H,Davison PA,Xu J,Huang WE

更新日期：2012-07-20 00:00:00

abstract：:Agricultural soils are often cocontaminated with multiple pesticides. Unfortunately, microorganisms isolated from natural environments do not possess the ability to simultaneously degrade different classes of pesticides. Currently, we can use the approaches of synthetic biology to create a strain endowed with various ...

journal_title：ACS synthetic biology

authors： Gong T,Liu R,Zuo Z,Che Y,Yu H,Song C,Yang C

更新日期：2016-05-20 00:00:00

abstract：:2-Pyrone-4,6-dicarboxylic acid (PDC) is a pseudoaromatic dicarboxylic acid and is a promising biobased building block chemical that can be used to make diverse polyesters with novel functionalities. In this study, Escherichia coli was metabolically engineered to produce PDC from glucose. First, an efficient biosynthet...

journal_title：ACS synthetic biology

authors： Luo ZW,Kim WJ,Lee SY

更新日期：2018-09-21 00:00:00

abstract：:The engineering of microbial metabolic pathways over the last two decades has led to numerous examples of cell factories used for the production of small molecules. These molecules have an array of utility in commercial industries and as in situ expressed biomarkers or therapeutics in microbial applications. While mos...

journal_title：ACS synthetic biology

authors： Chien T,Jones DR,Danino T

更新日期：2021-01-15 00:00:00

abstract：:Amyloids are highly ordered, hierarchal protein nanoassemblies. Functional amyloids in bacterial biofilms, such as Escherichia coli curli fibers, are formed by the polymerization of monomeric proteins secreted into the extracellular space. Curli is synthesized by living cells, is primarily composed of the major curlin...

journal_title：ACS synthetic biology

authors： Seker UO,Chen AY,Citorik RJ,Lu TK

更新日期：2017-02-17 00:00:00

abstract：:Secondary structure formation of mRNA, caused by desynchronization of transcription and translation, is known to impact gene expression in vivo. Yet, inactivation of mRNA by secondary structures in cell-free protein expression is frequently overlooked. Transcription and translation rates are often not highly synchroni...

journal_title：ACS synthetic biology

authors： Hansen MM,Ventosa Rosquelles M,Yelleswarapu M,Maas RJ,van Vugt-Jonker AJ,Heus HA,Huck WT

更新日期：2016-12-16 00:00:00