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Synthesis and Characterization of Heterodimers and Fluorescent Nisin Species by Incorporation of Methionine Analogues and Subsequent Click Chemistry.

Abstract：

:Noncanonical amino acids form a highly diverse pool of building blocks that can render unique physicochemical properties to peptides and proteins. Here, four methionine analogues with unsaturated and varying side chain lengths were successfully incorporated at four different positions in nisin in Lactococcus lactis through force feeding. This approach allows for residue-specific incorporation of methionine analogues into nisin to expand their structural diversity and alter their activity profiles. Moreover, the insertion of methionine analogues with biorthogonal chemical reactivity, e.g., azidohomoalanine and homopropargylglycine, provides the opportunity for chemical coupling to functional moieties and fluorescent probes as well as for intermolecular coupling of nisin variants. All resulting nisin conjugates retained antimicrobial activity, which substantiates the potential of this method as a tool to further study its localization and mode of action.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Deng J,Viel JH,Chen J,Kuipers OP

doi

10.1021/acssynbio.0c00308

subject

Has Abstract

pub_date

2020-09-18 00:00:00

pages

2525-2536

issue

9

issn

2161-5063

journal_volume

9

pub_type

杂志文章

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