当前位置： SCI文献检索 > ACS Synthetic Biology期刊下所有文献 > A Lambda Red and FLP/FRT-Mediated Site-Specific Recombination System in Komagataeibacter xylinus and Its Application to Enhance the Productivity of Bacterial Cellulose.

A Lambda Red and FLP/FRT-Mediated Site-Specific Recombination System in Komagataeibacter xylinus and Its Application to Enhance the Productivity of Bacterial Cellulose.

Abstract：

:Komagataeibacter xylinus has received increasing attention as an important microorganism for the conversion of several carbon sources to bacterial cellulose (BC). However, BC productivity has been impeded by the lack of efficient genetic engineering techniques. In this study, a lambda Red and FLP/FRT-mediated site-specific recombination system was successfully established in Komagataeibacter xylinus. Using this system, the membrane bound gene gcd, a gene that encodes glucose dehydrogenase, was knocked out to reduce the modification of glucose to gluconic acid. The engineered strain could not produce any gluconic acid and presented a decreased bacterial cellulose (BC) production due to its restricted glucose utilization. To address this problem, the gene of glucose facilitator protein (glf; ZMO0366) was introduced into the knockout strain coupled with the overexpression of the endogenous glucokinase gene (glk). The BC yield of the resultant strain increased by 63.63-173.68%, thus reducing the production cost.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Liu LP,Yang X,Zhao XJ,Zhang KY,Li WC,Xie YY,Jia SR,Zhong C

doi

10.1021/acssynbio.0c00450

subject

Has Abstract

pub_date

2020-11-20 00:00:00

pages

3171-3180

issue

11

issn

2161-5063

journal_volume

9

pub_type

杂志文章

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