A Genetically Encoded Protein Polymer for Uranyl Binding and Extraction Based on the SpyTag-SpyCatcher Chemistry.


:A defining goal of synthetic biology is to develop biomaterials with superior performance and versatility. Here we introduce a purely genetically encoded and self-assembling biopolymer based on the SpyTag-SpyCatcher chemistry. We show the application of this polymer for highly efficient uranyl binding and extraction from aqueous solutions, by embedding two functional modules-the superuranyl binding protein and the monomeric streptavidin-to the polymer via genetic fusion. We further provide a modeling strategy for predicting the polymer's physical properties, and experimentally demonstrate the autosecretion of component monomers from bacterial cells. The potential of multifunctionalization, in conjunction with the genetic design and production pipeline, underscores the advantage of the SpyTag-SpyCatcher biopolymers for applications beyond trace metal enrichment and environmental remediation.


ACS Synth Biol


ACS synthetic biology


Yang X,Wei J,Wang Y,Yang C,Zhao S,Li C,Dong Y,Bai K,Li Y,Teng H,Wang D,Lyu N,Li J,Chang X,Ning X,Ouyang Q,Zhang Y,Qian L




Has Abstract


2018-10-19 00:00:00










  • Analog Computation by DNA Strand Displacement Circuits.

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Song T,Garg S,Mokhtar R,Bui H,Reif J

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  • Real-time mRNA measurement during an in vitro transcription and translation reaction using binary probes.

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    journal_title:ACS synthetic biology

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    authors: Niederholtmeyer H,Xu L,Maerkl SJ

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    authors: Xue X,Wang T,Jiang P,Shao Y,Zhou M,Zhong L,Wu R,Zhou J,Xia H,Zhao G,Qin Z

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  • Minimization of Elements for Isothermal DNA Replication by an Evolutionary Approach.

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    authors: Okauchi H,Sakatani Y,Otsuka K,Ichihashi N

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    authors: Brady JR,Tan MC,Whittaker CA,Colant NA,Dalvie NC,Love KR,Love JC

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    authors: Nilgiriwala KS,Jiménez J,Rivera PM,Del Vecchio D

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    journal_title:ACS synthetic biology

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    authors: He BB,Bu XL,Zhou T,Li SM,Xu MJ,Xu J

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    journal_title:ACS synthetic biology

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    authors: Xiong X,Zhang Y,Yan J,Jain S,Chee S,Ren B,Zhao H

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    journal_title:ACS synthetic biology

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    authors: Sleight SC,Sauro HM

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Roehner N,Myers CJ

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  • Biosynthetic Routes for Producing Various Fucosyl-Oligosaccharides.

    abstract::Fucosyl-oligosaccharides (FOSs) play physiologically important roles as prebiotics, neuronal growth factors, and inhibitors of enteropathogens. However, challenges in designed synthesis and mass production of FOSs hamper their industrial applications. Here, we report flexible biosynthetic routes to produce various FOS...

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    authors: Yun EJ,Liu JJ,Lee JW,Kwak S,Yu S,Kim KH,Jin YS

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    pub_type: 杂志文章


    authors: Mundt M,Anders A,Murray SM,Sourjik V

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    pub_type: 杂志文章


    authors: Sukovich DJ,Modavi C,de Raad M,Prince RN,Anderson JC

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    authors: Pothoulakis G,Ceroni F,Reeve B,Ellis T

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    authors: Mukai T,Yoneji T,Yamada K,Fujita H,Nara S,Su'etsugu M

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Sauer C,Ver Loren van Themaat E,Boender LGM,Groothuis D,Cruz R,Hamoen LW,Harwood CR,van Rij T

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    authors: Xu K,Tong Y,Li Y,Tao J,Li J,Zhou J,Liu S

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Thompson MG,Costello Z,Hummel NFC,Cruz-Morales P,Blake-Hedges JM,Krishna RN,Skyrud W,Pearson AN,Incha MR,Shih PM,Garcia-Martin H,Keasling JD

    更新日期:2019-10-18 00:00:00

  • Single Day Construction of Multigene Circuits with 3G Assembly.

    abstract::The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting step of the prototyping cycle for synthetic biology. Recently developed DNA assembly methods such as isothermal assembly and type IIS restriction enzyme systems t...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Halleran AD,Swaminathan A,Murray RM

    更新日期:2018-05-18 00:00:00

  • A Novel Approach for Gene Expression Optimization through Native Promoter and 5' UTR Combinations Based on RNA-seq, Ribo-seq, and TSS-seq of Streptomyces coelicolor.

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    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Yi JS,Kim MW,Kim M,Jeong Y,Kim EJ,Cho BK,Kim BG

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    abstract::We have previously engineered green/red and red/far red photoreversible E. coli phytochrome and cyanobacteriochrome (CBCR) two-component systems (TCSs) and utilized them to program tailor-made gene expression signals for gene circuit characterization. Here, we transport the UV-violet/green photoreversible CBCR TCS Uir...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章


    authors: Ramakrishnan P,Tabor JJ

    更新日期:2016-07-15 00:00:00

  • The Fifth Annual Sc2.0 and Synthetic Genomes Conference: Synthetic Genomes in High Gear.

    abstract::The Sc2.0 project is perhaps the largest synthetic biology project in the public domain, and ultimately aims to construct a new version of the humble brewer's yeast, Saccharomyces cerevisiae. Each year, the Sc2.0 consortium gather to discuss progress in this ambitious project and highlight new developments at the fore...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章,评审


    authors: Walker RS,Cai Y

    更新日期:2016-09-16 00:00:00

  • Labeling RNAs in Live Cells Using Malachite Green Aptamer Scaffolds as Fluorescent Probes.

    abstract::RNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamer-fluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA),...

    journal_title:ACS synthetic biology

    pub_type: 信件


    authors: Yerramilli VS,Kim KH

    更新日期:2018-03-16 00:00:00