Abstract:
:Enoyl-acyl carrier protein reductase (ENR) catalyzes the last reduction step in the bacterial type II fatty acid biosynthesis cycle. ENRs include FabI, FabL, FabL2, FabK, and FabV. Previously, we reported a unique triclosan (TCL) resistant ENR homolog that was predominant in obligate intracellular pathogenic bacteria and Apicomplexa. Herein, we report the biochemical and structural basis of TCL resistance in this novel ENR. The purified protein revealed NADH-dependent ENR activity and shared similarity to prototypic FabI. Thus, this metagenome-derived ENR was designated FabI2. Unlike other prototypic bacterial ENRs with the YX6K type catalytic domain, FabI2 possessed a unique YX7K type catalytic domain. Computational modeling followed by site-directed mutagenesis revealed that mild resistance (20 µg/ml of minimum inhibitory concentration) of FabI2 to TCL was confined to the relatively less bulky side chain of A128. Substitution of A128 in FabI2 with bulky valine (V128) elevated TCL resistance. Phylogenetic analysis further suggested that the novel FabI2 and prototypical FabI evolved from a common short-chain dehydrogenase reductase family. To our best knowledge, FabI2 is the only known ENR shared by intracellular pathogenic prokaryotes, intracellular pathogenic lower eukaryotes, and a few higher eukaryotes. This suggests that the ENRs of prokaryotes and eukaryotes diverged from a common ancestral ENR of FabI2.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Khan R,Zeb A,Choi K,Lee G,Lee KW,Lee SWdoi
10.1038/s41598-019-51895-2subject
Has Abstractpub_date
2019-10-28 00:00:00pages
15401issue
1issn
2045-2322pii
10.1038/s41598-019-51895-2journal_volume
9pub_type
杂志文章abstract::Faces presented upside-down are harder to recognize than presented right-side up, an effect known as the face inversion effect. With inversion the perceptual processing of the spatial relationship among facial parts is disrupted. Previous literature indicates a face inversion effect in chimpanzees toward familiar and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02504
更新日期:2013-01-01 00:00:00
abstract::It has been reported that the ATM kinase inhibitor KU60019 preferentially radiosensitizes orthotopic high grade gliomas (HGG) driven by established U87 and U1242 cell lines bearing specific TP53 mutations. We wished to determine whether those results could be extended to tumors driven by primary glioma initiating cell...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32578-w
更新日期:2018-09-21 00:00:00
abstract::A novel design of thin-film crystalline silicon solar cell (TF C-Si-SC) is proposed and numerically analyzed. The reported SC has 1.0 µm thickness of C-Si with embedded dielectric silicon dioxide nanowires (NWs). The introduced NWs increase the light scattering in the active layer which improves the optical path lengt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74453-7
更新日期:2020-10-16 00:00:00
abstract::Cathode materials always limit the performance of fuel cells while the commercial platinum-based catalysts hardly meet the requirements of low cost, durable and stable. Here a non-precious metal oxygen reduction reaction (ORR) electocatalyst based on titanium nitride/titanium carbonitride hierarchical structures (TNTC...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06712
更新日期:2014-10-22 00:00:00
abstract::Nano-scale investigations of bainitic structures formed at temperatures below 350 °C have shown that the bainitic ferrite lattice is super-saturated in carbon. A high density of intrinsic defects would be playing a part in the carbon-supersaturation levels detected. In this work, the role of C-vacancy complexes on car...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57469-x
更新日期:2020-01-16 00:00:00
abstract::In this study we describe a new methodology to physically probe individual complexes formed between proteins and DNA. By combining nanoscale, high speed physical force measurement with sensitive fluorescence imaging we investigate the complex formed between the prokaryotic DNA repair protein UvrA2 and DNA. This approa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18486
更新日期:2015-12-22 00:00:00
abstract::Nitrous oxide (N2O) is a potent greenhouse gas. In North China, vegetable fields are amended with high levels of N fertilizer and irrigation water, which causes massive N2O flux. The aim of this study was to determine the contribution of microbial processes to N2O production and characterize isotopic signature effects...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29257
更新日期:2016-07-08 00:00:00
abstract::Tissue-resident memory T (TRM) cells are characterized by their surface expression of CD69 and can be subdivided in CD103+ and CD103- TRM cells. The origin and functional characteristics of TRM cells in the renal allograft are largely unknown. To determine these features we studied TRM cells in transplant nephrectomie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42401-9
更新日期:2019-04-12 00:00:00
abstract::Olive oil pomace (OOP) is a bio-waste rich in highly soluble polyphenols. OOP has been proposed as an additive in ruminant feeding to modulate rumen fermentations. Three groups of ewes were fed the following different diets: a control diet and two diets supplemented with OOP, obtained with a two-phase (OOP2) or three-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26713-w
更新日期:2018-05-31 00:00:00
abstract::Silicene is an exciting two-dimensional material that shares many of graphene's electronic properties, but differs in its structural buckling. This buckling allows opening a bandgap in silicene through the application of a perpendicular electric field. Here we show that this buckling also enables highly effective modu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14815
更新日期:2015-10-06 00:00:00
abstract::We analyze the ferroelectric domain-wall induced negative capacitance (NC) effect in Metal-FE-Insulator-Metal (MFIM) and Metal-FE-Insulator-Semiconductor (MFIS) stacks through phase-field simulations by self-consistently solving time-dependent Ginzburg Landau equation, Poisson's equation and semiconductor charge equat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66313-1
更新日期:2020-06-23 00:00:00
abstract::We organized a crowdsourcing experiment in the form of a snapshot sampling campaign to assess the spatial distribution of nitrogen solutes, namely, nitrate, ammonium and dissolved organic nitrogen (DON), in German surface waters. In particular, we investigated (i) whether crowdsourcing is a reasonable sampling method ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16503
更新日期:2015-11-12 00:00:00
abstract::Traditional diagnostic detection of chronic wasting disease (CWD) relies on immunodetection of misfolded CWD prion protein (PrP(CWD)) by western blotting, ELISA, or immunohistochemistry (IHC). These techniques require separate sample collections (frozen and fixed) which may result in discrepancies due to variation in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25098
更新日期:2016-05-09 00:00:00
abstract::Prior to this study, complete mitochondrial genomes from Order Thysanoptera were restricted to a single family, the Thripidae, resulting in a biased view of their evolution. Here we present the sequences for the mitochondrial genomes of four additional thrips species, adding three extra families and an additional subf...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57705-4
更新日期:2020-01-20 00:00:00
abstract::Knowledge of the direct role humans have had in changing the landscape requires the perspective of historical and archaeological sources, as well as climatic and ecologic processes, when interpreting paleoecological records. People directly impact land at the local scale and land use decisions are strongly influenced ...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-018-20286-4
更新日期:2018-02-01 00:00:00
abstract::The formation of OD (order-disorder) phases of the LPSO (long-period stacking ordered)-type in Mg-Al-RE (RE (rare earth) = Y, La, Ce, Nd, Sm, Dy, Ho, Er and Yb) ternary systems has been investigated for both as-solidified and annealed conditions. The OD phase is found to form in those systems with RE = Y, Nd, Sm, Dy, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12506-0
更新日期:2017-09-25 00:00:00
abstract::The metal-free nitrogen-doped graphitic-carbon@graphene (Ng-C@G) is prepared from a composite of polyaniline and graphene by a facile polymerization following by pyrolysis for electrochemical oxygen reduction reaction (ORR). Pyrolysis creates a sponge-like with ant-cave-architecture in the polyaniline derived nitrogen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68260-3
更新日期:2020-07-24 00:00:00
abstract::Treatment of both-column fractures with posterior wall involvement is still a controversial topic. This type of posterior wall fracture is different from isolated acetabular posterior wall fracture (AO/OTA62-A1). The aim of this study is to compare the morphology of the posterior wall fragments of these two fracture p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56838-5
更新日期:2019-12-27 00:00:00
abstract::Hypoxia is a recognized characteristic of tumors that influences efficacy of radiotherapy (RT). Photoacoustic imaging (PAI) is a relatively new imaging technique that exploits the optical characteristics of hemoglobin to provide information on tissue oxygenation. In the present study, PAI based measures of tumor oxyge...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21237
更新日期:2016-02-17 00:00:00
abstract::Aneurysmal subarachnoid hemorrhage (aSAH)-induced cerebral vasospasm and delayed ischemic neurological deficit (DIND) are the major causes of morbidity and mortality in patients with aSAH. The effects of statins-use for patients with aSAH remain controversial. Here,a total of 249 patients from six randomized controlle...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/srep04573
更新日期:2014-04-25 00:00:00
abstract::Atmosphere-ocean coupling effect on the frequency distribution of tropical cyclones (TCs) and its future change is studied using an atmosphere and ocean coupled general circulation model (AOGCM). In the present climate simulation, the atmosphere-ocean coupling in the AOGCM improves biases in the AGCM such as the polew...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29800
更新日期:2016-07-15 00:00:00
abstract::Gold nanoparticles (GNPs) have been demonstrated as effective radiosensitizing agents in a range of preclinical models using broad field sources of various energies. This study aimed to distinguish between these mechanisms by applying subcellular targeting using a soft X-ray microbeam in combination with GNPs. DNA dam...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44752
更新日期:2017-03-16 00:00:00
abstract::We characterized an anti-cancer fusion protein consisting of anthrax lethal factor (LF) and the catalytic domain of Pseudomonas exotoxin A by (i) mutating the N-terminal amino acids and by (ii) reductive methylation to dimethylate all lysines. Dimethylation of lysines was achieved quantitatively and specifically witho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04754
更新日期:2014-04-23 00:00:00
abstract::In this study, we analyze the role of different structural variants of proteins in the speciation processes. We separate human and mouse proteomes (taken as a reference) into three previously defined variants of disorder: ordered proteins (ORDPs), structured proteins with intrinsically disordered protein regions (IDPR...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61466-5
更新日期:2020-03-11 00:00:00
abstract::This study investigates the relationship between objective cognitive impairment (OCI), subjective cognitive complaints and depressive symptoms in men and women with classical and non-classical Fabry disease (FD). Cognitive functioning was assessed using a neuropsychological test battery, subjective cognitive complaint...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37320-0
更新日期:2019-01-17 00:00:00
abstract::The protection of tropical forests is one of the most urgent issues in conservation biology because of the rapid deforestation that has occurred over the last 50 years. Even in protected forests, the anthropogenic effects from newly expanding villages such as harvesting of medicinal plants, pasturing cattle and forest...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14689
更新日期:2015-10-05 00:00:00
abstract::Individuals with lower limb amputation need a secure suspension system for their prosthetic devices. A new coupling system was developed that is capable of suspending the prosthesis. The system's safety is ensured through an acoustic alarm system. This article explains how the system works and provides an in vivo eval...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02270
更新日期:2013-01-01 00:00:00
abstract::Epidemiological studies have shown that atherosclerotic risk factors accelerate the pathological process underlying Alzheimer's disease (AD) via chronic cerebral hypoperfusion. In this study, we aimed to clarify the mechanisms by which cerebral hypoperfusion may exacerbate AD pathology. We applied bilateral common car...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39494-7
更新日期:2019-02-26 00:00:00
abstract::WD40 proteins belong to a big protein family with members identified in every eukaryotic proteome. However, WD40 proteins were only reported in a few prokaryotic proteomes. Using WDSP ( http://wu.scbb.pkusz.edu.cn/wdsp/ ), a prediction tool, we identified thousands of prokaryotic WD40 proteins, among which few protein...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31140-y
更新日期:2018-08-28 00:00:00
abstract::Remarkable improvements in both structural and optical properties of wafer-scale hexagonal boron nitride (h-BN) films grown by metal-organic chemical vapor deposition (MOCVD) enabled by high-temperature post-growth annealing is presented. The enhanced crystallinity and homogeneity of the MOCVD-grown h-BN films grown a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47093-9
更新日期:2019-07-22 00:00:00