Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction.

abstract：:Transcription at individual genes in single cells is often pulsatile and stochastic. A key question emerges regarding how this behaviour contributes to tissue phenotype, but it has been a challenge to quantitatively analyse this in living cells over time, as opposed to studying snap-shots of gene expression state. We ...

journal_title：eLife

authors： Featherstone K,Hey K,Momiji H,McNamara AV,Patist AL,Woodburn J,Spiller DG,Christian HC,McNeilly AS,Mullins JJ,Finkenstädt BF,Rand DA,White MR,Davis JR

更新日期：2016-02-01 00:00:00

abstract：:Brain connectivity maps display a delicate balance between individual variation and stereotypy, suggesting the existence of dedicated mechanisms that simultaneously permit and limit individual variation. We show that during the development of the Drosophila central nervous system, mutual inhibition among groups of nei...

journal_title：eLife

authors： Langen M,Koch M,Yan J,De Geest N,Erfurth ML,Pfeiffer BD,Schmucker D,Moreau Y,Hassan BA

更新日期：2013-03-05 00:00:00

abstract：:Motor cortex (M1) has lateralized outputs, yet neurons can be active during movements of either arm. What is the nature and role of activity across the two hemispheres? We recorded muscles and neurons bilaterally while monkeys cycled with each arm. Most neurons were active during movement of either arm. Responses were...

journal_title：eLife

authors： Ames KC,Churchland MM

更新日期：2019-10-09 00:00:00

abstract：:Coronaries are essential for myocardial growth and heart function. Notch is crucial for mouse embryonic angiogenesis, but its role in coronary development remains uncertain. We show Jag1, Dll4 and activated Notch1 receptor expression in sinus venosus (SV) endocardium. Endocardial Jag1 removal blocks SV capillary sprou...

journal_title：eLife

authors： Travisano SI,Oliveira VL,Prados B,Grego-Bessa J,Piñeiro-Sabarís R,Bou V,Gómez MJ,Sánchez-Cabo F,MacGrogan D,de la Pompa JL

更新日期：2019-12-04 00:00:00

abstract：:In bacteria, multicellular behaviors are regulated by cell-cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imagin...

journal_title：eLife

authors： Ducret A,Fleuchot B,Bergam P,Mignot T

更新日期：2013-07-23 00:00:00

abstract：:Some cortical neurons receive highly selective thalamocortical (TC) input, but others do not. Here, we examine connectivity of single thalamic neurons (lateral geniculate nucleus, LGN) onto putative fast-spike inhibitory interneurons in layer 4 of rabbit visual cortex. We show that three 'rules' regulate this connecti...

journal_title：eLife

authors： Bereshpolova Y,Hei X,Alonso JM,Swadlow HA

更新日期：2020-12-08 00:00:00

abstract：:Centering and decentering of cellular components is essential for internal organization of cells and their ability to perform basic cellular functions such as division and motility. How cells achieve proper localization of their organelles is still not well-understood, especially in large cells such as oocytes. Here, ...

journal_title：eLife

authors： Ierushalmi N,Malik-Garbi M,Manhart A,Abu Shah E,Goode BL,Mogilner A,Keren K

更新日期：2020-04-21 00:00:00

abstract：:Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering of electrically and chemically responsive, contractile human muscle tissues ('myobundles') using primar...

journal_title：eLife

authors： Madden L,Juhas M,Kraus WE,Truskey GA,Bursac N

更新日期：2015-01-09 00:00:00

abstract：:Post-transcriptional control by small regulatory RNA (sRNA) is critical for rapid adaptive processes. sRNAs can directly modulate mRNA degradation in Proteobacteria without interfering with translation. However, Firmicutes have a fundamentally different set of ribonucleases for mRNA degradation and whether sRNAs can r...

journal_title：eLife

authors： Durand S,Braun F,Helfer AC,Romby P,Condon C

更新日期：2017-04-24 00:00:00

abstract：:Bone destruction relies on interactions between bone and immune cells. Bone-resorbing osteoclasts (OCLs) were recently identified as innate immune cells activating T cells toward tolerance or inflammation. Thus, pathological bone destruction not only relies on increased osteoclast differentiation, but also on the pres...

journal_title：eLife

authors： Madel MB,Ibáñez L,Ciucci T,Halper J,Rouleau M,Boutin A,Hue C,Duroux-Richard I,Apparailly F,Garchon HJ,Wakkach A,Blin-Wakkach C

更新日期：2020-05-13 00:00:00

abstract：:Responses of sensory neurons are often modeled using a weighted combination of rectified linear subunits. Since these subunits often cannot be measured directly, a flexible method is needed to infer their properties from the responses of downstream neurons. We present a method for maximum likelihood estimation of subu...

journal_title：eLife

authors： Shah NP,Brackbill N,Rhoades C,Kling A,Goetz G,Litke AM,Sher A,Simoncelli EP,Chichilnisky EJ

更新日期：2020-03-09 00:00:00

abstract：:bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Fu...

journal_title：eLife

authors： Trovisco V,Belaya K,Nashchekin D,Irion U,Sirinakis G,Butler R,Lee JJ,Gavis ER,St Johnston D

更新日期：2016-10-28 00:00:00

abstract：:Though neurotransmitters are essential elements in neuronal signal transduction, techniques for in vivo analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an active sen...

journal_title：eLife

authors： Xie K,Wang N,Lin X,Wang Z,Zhao X,Fang P,Yue H,Kim J,Luo J,Cui S,Yan F,Shi P

更新日期：2020-02-11 00:00:00

abstract：:We determined differential gene expression in response to high glucose in lymphoblastoid cell lines derived from matched individuals with type 1 diabetes with and without retinopathy. Those genes exhibiting the largest difference in glucose response were assessed for association with diabetic retinopathy in a genome-w...

journal_title：eLife

authors： Skol AD,Jung SC,Sokovic AM,Chen S,Fazal S,Sosina O,Borkar PP,Lin A,Sverdlov M,Cao D,Swaroop A,Bebu I,DCCT\/EDIC Study group.,Stranger BE,Grassi MA

更新日期：2020-11-09 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental pattern...

journal_title：eLife

authors： Koromila T,Gao F,Iwasaki Y,He P,Pachter L,Gergen JP,Stathopoulos A

更新日期：2020-07-23 00:00:00

abstract：:Mitochondrial dysfunction is associated with activation of the integrated stress response (ISR) but the underlying triggers remain unclear. We systematically combined acute mitochondrial inhibitors with genetic tools for compartment-specific NADH oxidation to trace mechanisms linking different forms of mitochondrial d...

journal_title：eLife

authors： Mick E,Titov DV,Skinner OS,Sharma R,Jourdain AA,Mootha VK

更新日期：2020-05-28 00:00:00

abstract：:Larval recruitment, the transition of pelagic larvae into reef-associated juveniles, is a critical step for the resilience of marine fish populations but its molecular control is unknown. Here, we investigate whether thyroid-hormones (TH) and their receptors (TR) coordinate the larval recruitment of the coral-reef-fis...

journal_title：eLife

authors： Holzer G,Besson M,Lambert A,François L,Barth P,Gillet B,Hughes S,Piganeau G,Leulier F,Viriot L,Lecchini D,Laudet V

更新日期：2017-10-30 00:00:00

abstract：:Ribonucleotide reductases (RNRs) convert ribonucleotides into deoxyribonucleotides, a reaction essential for DNA replication and repair. Human RNR requires two subunits for activity, the α subunit contains the active site, and the β subunit houses the radical cofactor. Here, we present a 3.3-Å resolution structure by ...

journal_title：eLife

authors： Brignole EJ,Tsai KL,Chittuluru J,Li H,Aye Y,Penczek PA,Stubbe J,Drennan CL,Asturias F

更新日期：2018-02-20 00:00:00

abstract：:Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a ph...

journal_title：eLife

authors： Bayer EA,Stecky RC,Neal L,Katsamba PS,Ahlsen G,Balaji V,Hoppe T,Shapiro L,Oren-Suissa M,Hobert O

更新日期：2020-10-06 00:00:00

abstract：:New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic ent...

journal_title：eLife

authors： Dirks PH,Roberts EM,Hilbert-Wolf H,Kramers JD,Hawks J,Dosseto A,Duval M,Elliott M,Evans M,Grün R,Hellstrom J,Herries AI,Joannes-Boyau R,Makhubela TV,Placzek CJ,Robbins J,Spandler C,Wiersma J,Woodhead J,Berger LR

更新日期：2017-05-09 00:00:00

abstract：:The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing regulation and mediates interactions with components of the U4/U...

journal_title：eLife

authors： Mourão A,Bonnal S,Soni K,Warner L,Bordonné R,Valcárcel J,Sattler M

更新日期：2016-11-29 00:00:00

abstract：:Understanding how genes and experience work in concert to generate phenotypic variability will provide a better understanding of individuality. Here, we considered this in the main olfactory epithelium, a chemosensory structure with over a thousand distinct cell types in mice. We identified a subpopulation of olfactor...

journal_title：eLife

authors： Vihani A,Hu XS,Gundala S,Koyama S,Block E,Matsunami H

更新日期：2020-11-24 00:00:00

abstract：:Glucocorticoid receptors (GR) have diverse functions relevant to maintenance of homeostasis and adaptation to environmental challenges. Understanding the importance of tissue-specific GR function in physiology and behavior has been hampered by near-ubiquitous localization in brain and body. Here we use CRISPR/Cas9 gen...

journal_title：eLife

authors： Scheimann JR,Moloney RD,Mahbod P,Morano RL,Fitzgerald M,Hoskins O,Packard BA,Cotella EM,Hu YC,Herman JP

更新日期：2019-07-22 00:00:00

abstract：:Sensory deprivation during the post-natal 'critical period' leads to structural reorganization of the developing visual cortex. In adulthood, the visual cortex retains some flexibility and adapts to sensory deprivation. Here we show that short-term (2 hr) monocular deprivation in adult humans boosts the BOLD response ...

journal_title：eLife

authors： Binda P,Kurzawski JW,Lunghi C,Biagi L,Tosetti M,Morrone MC

更新日期：2018-11-26 00:00:00

abstract：:The IFNλ family of interferons controls the spread of viruses in the upper respiratory tract and transmission between mice. ...

journal_title：eLife

authors： Wack A

更新日期：2018-05-24 00:00:00

abstract：:Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly con...

journal_title：eLife

authors： Krejčová G,Danielová A,Nedbalová P,Kazek M,Strych L,Chawla G,Tennessen JM,Lieskovská J,Jindra M,Doležal T,Bajgar A

更新日期：2019-10-14 00:00:00

abstract：:Even when we are at rest, our spinal cords show spontaneous, yet well organised, fluctuations of activity that might reflect sensory and motor networks. ...

journal_title：eLife

authors： Eippert F,Tracey I

更新日期：2014-08-05 00:00:00

abstract：:The phylogenetic placement of the morphologically simple placozoans is crucial to understanding the evolution of complex animal traits. Here, we examine the influence of adding new genomes from placozoans to a large dataset designed to study the deepest splits in the animal phylogeny. Using site-heterogeneous substitu...

journal_title：eLife

authors： Laumer CE,Gruber-Vodicka H,Hadfield MG,Pearse VB,Riesgo A,Marioni JC,Giribet G

更新日期：2018-10-30 00:00:00

abstract：:Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the Saccharomyces cerevisiae kinetochore. Cooperation between these two complexes enhances kinetochore-microtubule coupling ...

journal_title：eLife

authors： Kim JO,Zelter A,Umbreit NT,Bollozos A,Riffle M,Johnson R,MacCoss MJ,Asbury CL,Davis TN

更新日期：2017-02-13 00:00:00